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The map label for this gene is nqo13 [H]

Identifier: 222525388

GI number: 222525388

Start: 2710560

End: 2712224

Strand: Reverse

Name: nqo13 [H]

Synonym: Chy400_2133

Alternate gene names: 222525388

Gene position: 2712224-2710560 (Counterclockwise)

Preceding gene: 222525389

Following gene: 222525387

Centisome position: 51.48

GC content: 59.58

Gene sequence:

>1665_bases
ATGAATCTGCCAGAGTATCTTATTCCTGCTGCTGATGGTATTCCGTGGCTCACCCTGTTGGTGCTGGCGCCATTGGCCGG
TATTGCGCTCATCGGGTTGGCGTGGCTGCTCAAGTTCAATGAGCAGACGGTGAAAGCTGGGGTGCTGGCCTGGACGGGTG
TGCCGTTGTTGCTGGCCGGCATGGTCTGGGCACGTTTCGATCCGCTGGCCGTGGCGAGCGGGCAGGGTGCAGTGCAATTG
GTTGAGCGTGTCCCCTGGATTCAGGCGATCCGGGTTGATTACTTTCTCGGCGTGGATGGGCTGAGCCTGCCGCTCGTGAT
CCTGACGGCAGTTATGACGCCGTTGGCCGTGATTGCCAGTTGGGGTGTACGTGAACGGTTGCATGCGCACCTAGCGTTGC
TGCTCTTACTCGAAGCGGCGATGCTCGGTTATTTTGTCGCGCTCGATTTCTTCTTCTTCTTCATCTTCTGGGAATTCAGC
CTCGTGCCGGCCTTCTTCCTGATCCAGGGCTGGGGGCGCGAACAACGCCGCTATGCCGCTTTCAAGTTTTTTGTTTACAC
GATGGCCGGCTCGTTGGGCATGCTCCTGCTCTTCCAGGTGATCTATCTGGCGATGCGCCAGGTCGGCATGCCTACGTTCG
ATCTGATTGCCCTGGGACGGCTGGGGCAGGGATTGCCGGTAGCCGGTGTCGAAGGCACCTTGCGCGATATTCTCTTTGCC
TATCTTGAGCAGATCGGTGTAACCAATGCCCTCGGTCGCTATCCCCTGCTCTACAGCAGTATTGCAATGTGGGCGATTTT
CATAGCGTTCGCGATCAAACTGGCGGTCTGGCCATTCCATACCTGGCTCCCCGACGCCTACGCCGAAGGGCCAACTGCGG
CGAGTATCCTGCTCTCGGCAGTGATGAGCAAGATGGGGGCGTATGGCATGCTCCGCCTCCTGCTGCCCTTTACGCCCGAC
GCCGCCCAATACTTTGCCCCGGTGCTGGCTGCGCTGGCCGTCGTCGGCGTCGTTGCCGGTGCATTTGGCGCCCTTGGTCA
GGTCAATGGTGACATCAAGCGCCTGATTGGGTATACCTCGATCAACCACATGGGCTATGTAATGCTGGCAATTGCCGGTG
CGGCGGCGGCAGGCGAAGCCGGGATCGATGCGCGGGCCAGTGCAATTAACGGTGCCCTGGTGCAAATGGTTGCTCATGGC
CTCAGCACAGGGGCGCTCTTCTACCTGGCCGGGGTGTTGTATGAGCGCACCGGGCGTTGGGAACTCAGTGGTTTAGGTGG
ACTGCGCGCCAGTGCGCCAACGTTTGCCGGCGTGATGGGCATTGCACTCTTCGCGAATCTGGGTTTGCCTGGACTGGCCG
GCTTTGTGGGTGAATTCTTCATCTTCCGTGGGGCGTGGGCAACCTTACCCTTCTTTACGGCGCTGGCCGTGATCGGACTG
GTGGTAACTGCGCTGGCGCTGTTGCTCATGTTCCAGCGCATTTTTCTGGGGCCGGCAACCGGCACACCGCGTTCTATTCC
CGATCTACGCCCACAAGAATTCTGGGCCATCGTGCCGATTCTGGTGCTGTCGTTGCTGATTGGTGTCTATCCCGGCCCGC
TGATTGGCCTGGGCAATGCCGCTGCCGGACAACTGGTCGCTATTTTTGTTCGTGCCCTGAGTTAG

Upstream 100 bases:

>100_bases
CCGTTTACAAACCGGCAAGGCACAGGATTATCTGATCTACGTCTTTGGTGGCTTGCTCGCACTGGCGATGTTCTGGCTGT
ATGTAGTTGGGCGGTAGCGT

Downstream 100 bases:

>100_bases
ACGCGAAGATTGTCATCTGCGGATGAATTGCACAAAGGTGACCCATGGGGTTGATAACGCTTTCCGCCGACATACCCTGG
TTGAGCCTGATCTGGCTCAG

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MNLPEYLIPAADGIPWLTLLVLAPLAGIALIGLAWLLKFNEQTVKAGVLAWTGVPLLLAGMVWARFDPLAVASGQGAVQL
VERVPWIQAIRVDYFLGVDGLSLPLVILTAVMTPLAVIASWGVRERLHAHLALLLLLEAAMLGYFVALDFFFFFIFWEFS
LVPAFFLIQGWGREQRRYAAFKFFVYTMAGSLGMLLLFQVIYLAMRQVGMPTFDLIALGRLGQGLPVAGVEGTLRDILFA
YLEQIGVTNALGRYPLLYSSIAMWAIFIAFAIKLAVWPFHTWLPDAYAEGPTAASILLSAVMSKMGAYGMLRLLLPFTPD
AAQYFAPVLAALAVVGVVAGAFGALGQVNGDIKRLIGYTSINHMGYVMLAIAGAAAAGEAGIDARASAINGALVQMVAHG
LSTGALFYLAGVLYERTGRWELSGLGGLRASAPTFAGVMGIALFANLGLPGLAGFVGEFFIFRGAWATLPFFTALAVIGL
VVTALALLLMFQRIFLGPATGTPRSIPDLRPQEFWAIVPILVLSLLIGVYPGPLIGLGNAAAGQLVAIFVRALS

Sequences:

>Translated_554_residues
MNLPEYLIPAADGIPWLTLLVLAPLAGIALIGLAWLLKFNEQTVKAGVLAWTGVPLLLAGMVWARFDPLAVASGQGAVQL
VERVPWIQAIRVDYFLGVDGLSLPLVILTAVMTPLAVIASWGVRERLHAHLALLLLLEAAMLGYFVALDFFFFFIFWEFS
LVPAFFLIQGWGREQRRYAAFKFFVYTMAGSLGMLLLFQVIYLAMRQVGMPTFDLIALGRLGQGLPVAGVEGTLRDILFA
YLEQIGVTNALGRYPLLYSSIAMWAIFIAFAIKLAVWPFHTWLPDAYAEGPTAASILLSAVMSKMGAYGMLRLLLPFTPD
AAQYFAPVLAALAVVGVVAGAFGALGQVNGDIKRLIGYTSINHMGYVMLAIAGAAAAGEAGIDARASAINGALVQMVAHG
LSTGALFYLAGVLYERTGRWELSGLGGLRASAPTFAGVMGIALFANLGLPGLAGFVGEFFIFRGAWATLPFFTALAVIGL
VVTALALLLMFQRIFLGPATGTPRSIPDLRPQEFWAIVPILVLSLLIGVYPGPLIGLGNAAAGQLVAIFVRALS
>Mature_554_residues
MNLPEYLIPAADGIPWLTLLVLAPLAGIALIGLAWLLKFNEQTVKAGVLAWTGVPLLLAGMVWARFDPLAVASGQGAVQL
VERVPWIQAIRVDYFLGVDGLSLPLVILTAVMTPLAVIASWGVRERLHAHLALLLLLEAAMLGYFVALDFFFFFIFWEFS
LVPAFFLIQGWGREQRRYAAFKFFVYTMAGSLGMLLLFQVIYLAMRQVGMPTFDLIALGRLGQGLPVAGVEGTLRDILFA
YLEQIGVTNALGRYPLLYSSIAMWAIFIAFAIKLAVWPFHTWLPDAYAEGPTAASILLSAVMSKMGAYGMLRLLLPFTPD
AAQYFAPVLAALAVVGVVAGAFGALGQVNGDIKRLIGYTSINHMGYVMLAIAGAAAAGEAGIDARASAINGALVQMVAHG
LSTGALFYLAGVLYERTGRWELSGLGGLRASAPTFAGVMGIALFANLGLPGLAGFVGEFFIFRGAWATLPFFTALAVIGL
VVTALALLLMFQRIFLGPATGTPRSIPDLRPQEFWAIVPILVLSLLIGVYPGPLIGLGNAAAGQLVAIFVRALS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=413, Percent_Identity=30.9927360774818, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1788613, Length=481, Percent_Identity=35.7588357588358, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1788827, Length=386, Percent_Identity=24.0932642487047, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI145693160, Length=276, Percent_Identity=28.9855072463768, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788831, Length=195, Percent_Identity=30.2564102564103, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI2367154, Length=201, Percent_Identity=29.3532338308458, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 59170; Mature: 59170

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHC
>Mature Secondary Structure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HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]