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The map label for this gene is ndhF [H]

Identifier: 222525389

GI number: 222525389

Start: 2712228

End: 2714426

Strand: Reverse

Name: ndhF [H]

Synonym: Chy400_2134

Alternate gene names: 222525389

Gene position: 2714426-2712228 (Counterclockwise)

Preceding gene: 222525390

Following gene: 222525388

Centisome position: 51.52

GC content: 58.89

Gene sequence:

>2199_bases
ATGGCGTTTTTTCTGCAATATGCGTGGCTGATTCCATTGCTGCCGTTACTGGCCAGCGCGCTCATTACGCTGACCCCGCT
GCGTCGGGCTGCGCTGGCAAGTGCGTGGCTGGCAACCGGGCTGATGATGGGGTCAACGGTTCTGGCCCTGGGTCTTCTGG
CCGCAGTTGCCGGCGGATGGGCGCTGCACGACGGACAACTGGTGCAACTTGCCGGCCATCACGGCGAACACGGTGCCTTC
ACCTTTCCCGAACCGAATGTCGTGCAGCGGTTCGTGTGGGCGCCAACCGGTGATACCGCATTACAGATGGGTATCTACAT
CGATGGCGCTGCTGCCGCAATGCTGGCGATGGTCACGATTGCCTCGACCTGCATTCATCTCTTTTCGATAGGCTACATGG
CGTCTCACCCGCGGCAGAGCCGCTTCTTCAGCTTTATCGCTCTCTTTACGGCGGCGATGTTGCTGATGAGCATGGCCGAT
AATCTGCTGCTCTTCTTCATGGCCTGGGAGATCATGGGTCTCTGTTCATACCTGTTGATCGGCTTTCTCTACGAGCGACC
CAGTGCAGCGCGGGCAGCGGTGAAGGCTTTTATCACTACCCGCATCGGGGATGTGCTGCTGCTGCTGGGTATTGTCTACC
TCTATGCCCAGGCAGGTGGGATTGGGTTTGGCAACGATGAAGGCGGTATCTTCTTTCCGCCACTGCTGGAACGATTGGCA
AGCGAAACCGGTGCCCTGGGCTTAAGTCACGCTGCCGGAATTGCACTGCTCCTGTTTGGCGGTACGGTAGGGAAATCGGC
CCAATTCCCGCTCCACGTCTGGTTGCCTGACGCAATGGAAGGCCCGACCCCGGTATCCGCACTCATTCACGCTGCAACGA
TGGTAGCGGCAGGTGTGTTCCTGGTGGCACGAACCTTCCCCATCTTTACTGCCGATCCGACAGCGCTGGGCGTGGTGGCC
TTTATTGGTGCCTTTACTGCTCTGTTTGCCGCCACCATCGCTATTGCCCAGTTCGACATCAAGCGCATTCTGGCCTACAG
CACGCTCTCGCAACTCGGCTTTATGGTGGCCGCACTCGGCGTAGGAGGATGGGTGGCGGCGATGTTCCATCTAATCACCC
ACGCCTTCTTTAAAGCGCTGCTCTTCCTCGGTTCAGGTTCGATCATTCACGCCATGGAACAACAGCCTGCGATCCGGCAG
ATTCACCATCACGACGAGTATGCTGCTCAGCAGACTGCCCAGGACATTCGCAATATGGGTGGCCTGCGCACGCGCCTGCC
GTGGACGTTCTGGACGTATACAGCCGGTTATCTGGCCCTGGCGGGGATCGTGCCGTTCGCCGGCTTTTGGAGCAAGGATG
AGATTCTGCTCGATGCGTTCAAGCACAATCTGGCAGTGTACCTGACGCTCAGCGCGGCTGCATTTCTGACTGCGGTGTAT
ATGACCCGCCAGTGGTGGCTGGTCTTTTTCGGGGAGTACCGTGGTGCGCAGCCGGTGGTGTTCGTTAATCCCGATGCACC
GGCATCGGCCTCTGACCACGATCATCACCACGACGACCATCAGCATGCCCACGACCACGGTGAGCACTGGCATGAAGACC
CACGGATGATCGCGCCGCTGGTGATACTGGCAAGTTTTGCCCTGACCGCCGGCGCACTCAATCTGCCCTTCGATCTCCCC
TTCGGTCACTGGCTGGCCGATGTGTGGGGGCAAGAGGCAGCGAAGCTCGATCCGCTGGTGGCCGGTCTCTCACTGCTGAT
TGCCATAGCCGGGATCGCCGCCGGCTGGTTGAGCTATCGGCACGCCTTCACCTCTGCCGATGACACCGATCCGCTGGCAG
CCCGCTTCCCAGCGTTATTTGCAGTTCTCCATGCCAAATACCGCATCGACGAATTGTATGCTGCAACCTTCGGCAGACTG
ACCCGTAGCCTGGCCGTGGCCTGGTCGTTGATCGACCGCCAGCTCATTGATCGCCTGATTGACGGTATGGGTCGCTTTAC
CTTCGGTTTGGGCAAAGTAAACTTCATCATCGATGACACGCTGTTGAACGATGGGCCAGACGGTTTGGCAAGTGGCACCC
AGGGCAGTGGCCGACAGGCGCGCCGTTTACAAACCGGCAAGGCACAGGATTATCTGATCTACGTCTTTGGTGGCTTGCTC
GCACTGGCGATGTTCTGGCTGTATGTAGTTGGGCGGTAG

Upstream 100 bases:

>100_bases
CAAACCGGTACTGATACGCACAGAACATTCAGAACAGAATTGCAGTTGGGTGGCAGGCTCGTACACTCGTGGTACGAGTT
GAGACGACGGGAATCGTACT

Downstream 100 bases:

>100_bases
CGTATGAATCTGCCAGAGTATCTTATTCCTGCTGCTGATGGTATTCCGTGGCTCACCCTGTTGGTGCTGGCGCCATTGGC
CGGTATTGCGCTCATCGGGT

Product: proton-translocating NADH-quinone oxidoreductase subunit L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MAFFLQYAWLIPLLPLLASALITLTPLRRAALASAWLATGLMMGSTVLALGLLAAVAGGWALHDGQLVQLAGHHGEHGAF
TFPEPNVVQRFVWAPTGDTALQMGIYIDGAAAAMLAMVTIASTCIHLFSIGYMASHPRQSRFFSFIALFTAAMLLMSMAD
NLLLFFMAWEIMGLCSYLLIGFLYERPSAARAAVKAFITTRIGDVLLLLGIVYLYAQAGGIGFGNDEGGIFFPPLLERLA
SETGALGLSHAAGIALLLFGGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVARTFPIFTADPTALGVVA
FIGAFTALFAATIAIAQFDIKRILAYSTLSQLGFMVAALGVGGWVAAMFHLITHAFFKALLFLGSGSIIHAMEQQPAIRQ
IHHHDEYAAQQTAQDIRNMGGLRTRLPWTFWTYTAGYLALAGIVPFAGFWSKDEILLDAFKHNLAVYLTLSAAAFLTAVY
MTRQWWLVFFGEYRGAQPVVFVNPDAPASASDHDHHHDDHQHAHDHGEHWHEDPRMIAPLVILASFALTAGALNLPFDLP
FGHWLADVWGQEAAKLDPLVAGLSLLIAIAGIAAGWLSYRHAFTSADDTDPLAARFPALFAVLHAKYRIDELYAATFGRL
TRSLAVAWSLIDRQLIDRLIDGMGRFTFGLGKVNFIIDDTLLNDGPDGLASGTQGSGRQARRLQTGKAQDYLIYVFGGLL
ALAMFWLYVVGR

Sequences:

>Translated_732_residues
MAFFLQYAWLIPLLPLLASALITLTPLRRAALASAWLATGLMMGSTVLALGLLAAVAGGWALHDGQLVQLAGHHGEHGAF
TFPEPNVVQRFVWAPTGDTALQMGIYIDGAAAAMLAMVTIASTCIHLFSIGYMASHPRQSRFFSFIALFTAAMLLMSMAD
NLLLFFMAWEIMGLCSYLLIGFLYERPSAARAAVKAFITTRIGDVLLLLGIVYLYAQAGGIGFGNDEGGIFFPPLLERLA
SETGALGLSHAAGIALLLFGGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVARTFPIFTADPTALGVVA
FIGAFTALFAATIAIAQFDIKRILAYSTLSQLGFMVAALGVGGWVAAMFHLITHAFFKALLFLGSGSIIHAMEQQPAIRQ
IHHHDEYAAQQTAQDIRNMGGLRTRLPWTFWTYTAGYLALAGIVPFAGFWSKDEILLDAFKHNLAVYLTLSAAAFLTAVY
MTRQWWLVFFGEYRGAQPVVFVNPDAPASASDHDHHHDDHQHAHDHGEHWHEDPRMIAPLVILASFALTAGALNLPFDLP
FGHWLADVWGQEAAKLDPLVAGLSLLIAIAGIAAGWLSYRHAFTSADDTDPLAARFPALFAVLHAKYRIDELYAATFGRL
TRSLAVAWSLIDRQLIDRLIDGMGRFTFGLGKVNFIIDDTLLNDGPDGLASGTQGSGRQARRLQTGKAQDYLIYVFGGLL
ALAMFWLYVVGR
>Mature_731_residues
AFFLQYAWLIPLLPLLASALITLTPLRRAALASAWLATGLMMGSTVLALGLLAAVAGGWALHDGQLVQLAGHHGEHGAFT
FPEPNVVQRFVWAPTGDTALQMGIYIDGAAAAMLAMVTIASTCIHLFSIGYMASHPRQSRFFSFIALFTAAMLLMSMADN
LLLFFMAWEIMGLCSYLLIGFLYERPSAARAAVKAFITTRIGDVLLLLGIVYLYAQAGGIGFGNDEGGIFFPPLLERLAS
ETGALGLSHAAGIALLLFGGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVARTFPIFTADPTALGVVAF
IGAFTALFAATIAIAQFDIKRILAYSTLSQLGFMVAALGVGGWVAAMFHLITHAFFKALLFLGSGSIIHAMEQQPAIRQI
HHHDEYAAQQTAQDIRNMGGLRTRLPWTFWTYTAGYLALAGIVPFAGFWSKDEILLDAFKHNLAVYLTLSAAAFLTAVYM
TRQWWLVFFGEYRGAQPVVFVNPDAPASASDHDHHHDDHQHAHDHGEHWHEDPRMIAPLVILASFALTAGALNLPFDLPF
GHWLADVWGQEAAKLDPLVAGLSLLIAIAGIAAGWLSYRHAFTSADDTDPLAARFPALFAVLHAKYRIDELYAATFGRLT
RSLAVAWSLIDRQLIDRLIDGMGRFTFGLGKVNFIIDDTLLNDGPDGLASGTQGSGRQARRLQTGKAQDYLIYVFGGLLA
LAMFWLYVVGR

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=425, Percent_Identity=35.7647058823529, Blast_Score=233, Evalue=4e-61,
Organism=Escherichia coli, GI1788614, Length=420, Percent_Identity=44.047619047619, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788829, Length=522, Percent_Identity=28.1609195402299, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788831, Length=364, Percent_Identity=31.5934065934066, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788827, Length=388, Percent_Identity=29.3814432989691, Blast_Score=123, Evalue=5e-29,
Organism=Escherichia coli, GI145693160, Length=265, Percent_Identity=29.811320754717, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1788613, Length=435, Percent_Identity=26.2068965517241, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI2367154, Length=380, Percent_Identity=24.2105263157895, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 79231; Mature: 79100

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFFLQYAWLIPLLPLLASALITLTPLRRAALASAWLATGLMMGSTVLALGLLAAVAGGW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALHDGQLVQLAGHHGEHGAFTFPEPNVVQRFVWAPTGDTALQMGIYIDGAAAAMLAMVTI
EECCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHH
ASTCIHLFSIGYMASHPRQSRFFSFIALFTAAMLLMSMADNLLLFFMAWEIMGLCSYLLI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFLYERPSAARAAVKAFITTRIGDVLLLLGIVYLYAQAGGIGFGNDEGGIFFPPLLERLA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHH
SETGALGLSHAAGIALLLFGGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVF
HHHCCCCHHHHHHHHHHEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LVARTFPIFTADPTALGVVAFIGAFTALFAATIAIAQFDIKRILAYSTLSQLGFMVAALG
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGWVAAMFHLITHAFFKALLFLGSGSIIHAMEQQPAIRQIHHHDEYAAQQTAQDIRNMG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
GLRTRLPWTFWTYTAGYLALAGIVPFAGFWSKDEILLDAFKHNLAVYLTLSAAAFLTAVY
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHH
MTRQWWLVFFGEYRGAQPVVFVNPDAPASASDHDHHHDDHQHAHDHGEHWHEDPRMIAPL
HHHHHHHHEEECCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHH
VILASFALTAGALNLPFDLPFGHWLADVWGQEAAKLDPLVAGLSLLIAIAGIAAGWLSYR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFTSADDTDPLAARFPALFAVLHAKYRIDELYAATFGRLTRSLAVAWSLIDRQLIDRLI
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGMGRFTFGLGKVNFIIDDTLLNDGPDGLASGTQGSGRQARRLQTGKAQDYLIYVFGGLL
HHCHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
ALAMFWLYVVGR
HHHHHHHHHHCC
>Mature Secondary Structure 
AFFLQYAWLIPLLPLLASALITLTPLRRAALASAWLATGLMMGSTVLALGLLAAVAGGW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALHDGQLVQLAGHHGEHGAFTFPEPNVVQRFVWAPTGDTALQMGIYIDGAAAAMLAMVTI
EECCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHH
ASTCIHLFSIGYMASHPRQSRFFSFIALFTAAMLLMSMADNLLLFFMAWEIMGLCSYLLI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFLYERPSAARAAVKAFITTRIGDVLLLLGIVYLYAQAGGIGFGNDEGGIFFPPLLERLA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHH
SETGALGLSHAAGIALLLFGGTVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVF
HHHCCCCHHHHHHHHHHEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LVARTFPIFTADPTALGVVAFIGAFTALFAATIAIAQFDIKRILAYSTLSQLGFMVAALG
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGGWVAAMFHLITHAFFKALLFLGSGSIIHAMEQQPAIRQIHHHDEYAAQQTAQDIRNMG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
GLRTRLPWTFWTYTAGYLALAGIVPFAGFWSKDEILLDAFKHNLAVYLTLSAAAFLTAVY
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHH
MTRQWWLVFFGEYRGAQPVVFVNPDAPASASDHDHHHDDHQHAHDHGEHWHEDPRMIAPL
HHHHHHHHEEECCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHH
VILASFALTAGALNLPFDLPFGHWLADVWGQEAAKLDPLVAGLSLLIAIAGIAAGWLSYR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFTSADDTDPLAARFPALFAVLHAKYRIDELYAATFGRLTRSLAVAWSLIDRQLIDRLI
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGMGRFTFGLGKVNFIIDDTLLNDGPDGLASGTQGSGRQARRLQTGKAQDYLIYVFGGLL
HHCHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
ALAMFWLYVVGR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]