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The map label for this gene is ndhD2 [H]

Identifier: 222525387

GI number: 222525387

Start: 2709004

End: 2710515

Strand: Reverse

Name: ndhD2 [H]

Synonym: Chy400_2132

Alternate gene names: 222525387

Gene position: 2710515-2709004 (Counterclockwise)

Preceding gene: 222525388

Following gene: 222525386

Centisome position: 51.44

GC content: 56.94

Gene sequence:

>1512_bases
ATGGGGTTGATAACGCTTTCCGCCGACATACCCTGGTTGAGCCTGATCTGGCTCAGTATGCTGGTGCCGGCCATCATTAT
TGCGTTTATTCCTGATCGTTACCCCGATCTGATGCGTTGGGTCGGGACAGGGTTTGCGCTCATCTCGCTTATCCTGTCGC
TCCTGGTCTTCCTGGCTTACGATCCGGTGCGCGGCGGTTTTCAGTTTGTCGAGTATCTCGACTGGATACCGCAGCTTGGG
ATCAGTTACCTGTTAGGGGCGGACGGCATCAATCTGCCGATGCTGCTCCTCAACGGTATCGTGATCTTTACCGGTGCCCT
GATGAGCTGGAATATTGAAGAGCGCATCAAAGAATATTGGGTCTGGTTGTTACTGCTGACAACCGGCGTCTATGGGGTGT
TTAGTGCGCTCGACCTCTTCCTCTTCTTCGTGTTCTACGAGCTGGCAGTGCTGCCGATGTACCTGCTGATCGGCATCTGG
GGGAGCACGCGCAAAGAGTACGGAGCAATGAAGCTGACCCTGTTCCTGATGGCGGGCAGTGCGCTGGTGATTATCGGCAT
GATCGGTCTCTATTTCGGCAGTGGCCTGCGCACCTTCAATATGCTGGTGCTGGCCGAACAAGGCTTCATTGCCGGCGATC
TGCAACGCCTGCTCTTCCCCGCGCTCTTCGTTGGCTTCGGAGTACTGGCCGGCATGTTCCCCTTTCACACCTGGAGTCCA
ACTGGACACGTGGCAGCACCGACGGCAGTCAGCATGTTGCACGCCGGCGTACTGATGAAACTGGGAGCCTACGGATGTCT
GCGGGCAGCTATGTGGTTGATGCCGATTGGTGCGCGCGATTGGCTGCCAATCGTCGTGATCCTGACCATCCTCAACGTGG
TGTACGGTGCCAGTATTGCGATGGTTCAGCGCGACGCGAAGTTCATCATCGGCTATTCATCGGTAAGCCACATGGGTCTG
GTGGTAATGGGGTTGGCTGCCGGAACGCAAATAGCAGTGATGGGCGCAGTCTTACAAATGTTTGCGCATGGCATTATGAC
GGCGCTCTTCTTTGCCGTGGTCGGGCGCATGATCTACGACCGAACCCATACCCGGCAGTTGCCTGAATTAGGTGGCCTGG
GCCGGGTGATGCCCTTTGCGGCTGCGATGTTTATTCTGGGTGGTCTCTCGTCAATGGGCATGCCGGGACTGGCCGGCTTC
TGGGCCGAGTTCAACATCTTCATCGGTGTCTGGGAACGCTACCCGCTGGTCGCAGTGATTGCGGCGTTGAGCATCCCGAT
CACCGGTGCCTATATTCTGCGGGCAGTCTGGGCCGTGTTCTACGACGAAGTCAAGAACCCCGAATTCTTGCATCTCCCCA
AACTCACCTGGCAAGAGTACAGCGGGGCACTGATCCTGGCACTGGTGCTGGTAGGGTTGGGCCTGTATCCGGCGTGGATG
ACAGAGTTGATTGATACCGGCGTGCGACCTTTAGTAGCGCGTCTCGGTGAAGTTATGGTGGCGATGCGCTGA

Upstream 100 bases:

>100_bases
TGGGCAATGCCGCTGCCGGACAACTGGTCGCTATTTTTGTTCGTGCCCTGAGTTAGACGCGAAGATTGTCATCTGCGGAT
GAATTGCACAAAGGTGACCC

Downstream 100 bases:

>100_bases
TTGGGAGAGACAAAGAGGGTCGTATGTTTCAACTGACCGACATCCCGCGCCTGTTACCTGAAATTTTGCTACTGGTGCTG
GCGCTGCTGGTACTGGGATC

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 2; NDH-1, chain 4 2 [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MGLITLSADIPWLSLIWLSMLVPAIIIAFIPDRYPDLMRWVGTGFALISLILSLLVFLAYDPVRGGFQFVEYLDWIPQLG
ISYLLGADGINLPMLLLNGIVIFTGALMSWNIEERIKEYWVWLLLLTTGVYGVFSALDLFLFFVFYELAVLPMYLLIGIW
GSTRKEYGAMKLTLFLMAGSALVIIGMIGLYFGSGLRTFNMLVLAEQGFIAGDLQRLLFPALFVGFGVLAGMFPFHTWSP
TGHVAAPTAVSMLHAGVLMKLGAYGCLRAAMWLMPIGARDWLPIVVILTILNVVYGASIAMVQRDAKFIIGYSSVSHMGL
VVMGLAAGTQIAVMGAVLQMFAHGIMTALFFAVVGRMIYDRTHTRQLPELGGLGRVMPFAAAMFILGGLSSMGMPGLAGF
WAEFNIFIGVWERYPLVAVIAALSIPITGAYILRAVWAVFYDEVKNPEFLHLPKLTWQEYSGALILALVLVGLGLYPAWM
TELIDTGVRPLVARLGEVMVAMR

Sequences:

>Translated_503_residues
MGLITLSADIPWLSLIWLSMLVPAIIIAFIPDRYPDLMRWVGTGFALISLILSLLVFLAYDPVRGGFQFVEYLDWIPQLG
ISYLLGADGINLPMLLLNGIVIFTGALMSWNIEERIKEYWVWLLLLTTGVYGVFSALDLFLFFVFYELAVLPMYLLIGIW
GSTRKEYGAMKLTLFLMAGSALVIIGMIGLYFGSGLRTFNMLVLAEQGFIAGDLQRLLFPALFVGFGVLAGMFPFHTWSP
TGHVAAPTAVSMLHAGVLMKLGAYGCLRAAMWLMPIGARDWLPIVVILTILNVVYGASIAMVQRDAKFIIGYSSVSHMGL
VVMGLAAGTQIAVMGAVLQMFAHGIMTALFFAVVGRMIYDRTHTRQLPELGGLGRVMPFAAAMFILGGLSSMGMPGLAGF
WAEFNIFIGVWERYPLVAVIAALSIPITGAYILRAVWAVFYDEVKNPEFLHLPKLTWQEYSGALILALVLVGLGLYPAWM
TELIDTGVRPLVARLGEVMVAMR
>Mature_502_residues
GLITLSADIPWLSLIWLSMLVPAIIIAFIPDRYPDLMRWVGTGFALISLILSLLVFLAYDPVRGGFQFVEYLDWIPQLGI
SYLLGADGINLPMLLLNGIVIFTGALMSWNIEERIKEYWVWLLLLTTGVYGVFSALDLFLFFVFYELAVLPMYLLIGIWG
STRKEYGAMKLTLFLMAGSALVIIGMIGLYFGSGLRTFNMLVLAEQGFIAGDLQRLLFPALFVGFGVLAGMFPFHTWSPT
GHVAAPTAVSMLHAGVLMKLGAYGCLRAAMWLMPIGARDWLPIVVILTILNVVYGASIAMVQRDAKFIIGYSSVSHMGLV
VMGLAAGTQIAVMGAVLQMFAHGIMTALFFAVVGRMIYDRTHTRQLPELGGLGRVMPFAAAMFILGGLSSMGMPGLAGFW
AEFNIFIGVWERYPLVAVIAALSIPITGAYILRAVWAVFYDEVKNPEFLHLPKLTWQEYSGALILALVLVGLGLYPAWMT
ELIDTGVRPLVARLGEVMVAMR

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=362, Percent_Identity=29.0055248618785, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI251831117, Length=176, Percent_Identity=26.1363636363636, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788613, Length=502, Percent_Identity=32.4701195219124, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788831, Length=353, Percent_Identity=28.0453257790368, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788827, Length=310, Percent_Identity=28.7096774193548, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788829, Length=317, Percent_Identity=26.4984227129338, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788614, Length=475, Percent_Identity=25.2631578947368, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693160, Length=400, Percent_Identity=26, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI2367154, Length=258, Percent_Identity=23.6434108527132, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 55282; Mature: 55151

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLITLSADIPWLSLIWLSMLVPAIIIAFIPDRYPDLMRWVGTGFALISLILSLLVFLAY
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC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HHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA