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The map label for this gene is ntrX [H]

Identifier: 222525284

GI number: 222525284

Start: 2564590

End: 2565219

Strand: Direct

Name: ntrX [H]

Synonym: Chy400_2025

Alternate gene names: 222525284

Gene position: 2564590-2565219 (Clockwise)

Preceding gene: 222525283

Following gene: 222525286

Centisome position: 48.67

GC content: 57.14

Gene sequence:

>630_bases
GTGCAAGAACCAATTCTCCTGGTGGAAGACGATCAAATCCTGCAACTCACCCTCACCACAATGCTGCGGCGCGACGGTTA
TGCCGTAGATACTGCACGCAACATTGCCGAAGCTCGTCTGGCGATCCTCTCGCGTCGGCCAAGAATCATTCTGCTTGACC
TCGGCTTACCCGATGGCAGTGGGTTTGATATTCTCAACTGGTTAAACCTGGAACCAGATCGGCCACTCATTATCGTCACG
ACTGCAAACGATTCGCCGAAAGCGGCGCTTGAAGCCCTGGGACTCGGTGCCTTCGACTACCTGACAAAACCGATCAACCA
CGAAACCCTCCGGTACACGCTCCGTCGTGCGGTCAGCTACGATCAGCTTCGGCAGCAAGTCCGTAACTACGAAGAATTAC
GCAGCGAAGTGGATGAAGCGCGTCTAACCGTGCGCAGCGCAGCTCACCATATCAGCCAGGCCTTAACGGTGATCATGGGA
GAAGCGCAACTGGTACGCGAAGAGATATCTGATCCAGCCCTCCGGGTCAGCCTGGATCGGATCGTGCGCATGGCCGAGGA
AGCAGCCCAGACCCTCGCTACACTGCGCACGAACCGCCTGCTTGCACCGCGCGAGGGGAGTGTTGAGTGA

Upstream 100 bases:

>100_bases
TAAGTGTGTACAATACACACAGGTTGAAGATTGGTTTAAGAATTACCAGGGCCGGCTCTCCTGTGGCCGGCGGCTGCCAC
TCACATGATGGGGTAATCCG

Downstream 100 bases:

>100_bases
GAGTCTGCTGCAAGACGTGCGCACCGTACAAGAACAATCATATGTCGCCAGTAGAAGTAAGGGCACGGCATGCCGTGCCC
CTACTGGTGTCCGGTGATCA

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MQEPILLVEDDQILQLTLTTMLRRDGYAVDTARNIAEARLAILSRRPRIILLDLGLPDGSGFDILNWLNLEPDRPLIIVT
TANDSPKAALEALGLGAFDYLTKPINHETLRYTLRRAVSYDQLRQQVRNYEELRSEVDEARLTVRSAAHHISQALTVIMG
EAQLVREEISDPALRVSLDRIVRMAEEAAQTLATLRTNRLLAPREGSVE

Sequences:

>Translated_209_residues
MQEPILLVEDDQILQLTLTTMLRRDGYAVDTARNIAEARLAILSRRPRIILLDLGLPDGSGFDILNWLNLEPDRPLIIVT
TANDSPKAALEALGLGAFDYLTKPINHETLRYTLRRAVSYDQLRQQVRNYEELRSEVDEARLTVRSAAHHISQALTVIMG
EAQLVREEISDPALRVSLDRIVRMAEEAAQTLATLRTNRLLAPREGSVE
>Mature_209_residues
MQEPILLVEDDQILQLTLTTMLRRDGYAVDTARNIAEARLAILSRRPRIILLDLGLPDGSGFDILNWLNLEPDRPLIIVT
TANDSPKAALEALGLGAFDYLTKPINHETLRYTLRRAVSYDQLRQQVRNYEELRSEVDEARLTVRSAAHHISQALTVIMG
EAQLVREEISDPALRVSLDRIVRMAEEAAQTLATLRTNRLLAPREGSVE

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1786911, Length=114, Percent_Identity=37.719298245614, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=177, Percent_Identity=25.9887005649718, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=33.59375, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787375, Length=105, Percent_Identity=36.1904761904762, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1786838, Length=114, Percent_Identity=37.719298245614, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87082012, Length=164, Percent_Identity=30.4878048780488, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 23546; Mature: 23546

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPILLVEDDQILQLTLTTMLRRDGYAVDTARNIAEARLAILSRRPRIILLDLGLPDGS
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
GFDILNWLNLEPDRPLIIVTTANDSPKAALEALGLGAFDYLTKPINHETLRYTLRRAVSY
CCCHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCH
DQLRQQVRNYEELRSEVDEARLTVRSAAHHISQALTVIMGEAQLVREEISDPALRVSLDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IVRMAEEAAQTLATLRTNRLLAPREGSVE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQEPILLVEDDQILQLTLTTMLRRDGYAVDTARNIAEARLAILSRRPRIILLDLGLPDGS
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
GFDILNWLNLEPDRPLIIVTTANDSPKAALEALGLGAFDYLTKPINHETLRYTLRRAVSY
CCCHHHCCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCH
DQLRQQVRNYEELRSEVDEARLTVRSAAHHISQALTVIMGEAQLVREEISDPALRVSLDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IVRMAEEAAQTLATLRTNRLLAPREGSVE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]