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The map label for this gene is thiED [H]

Identifier: 222524146

GI number: 222524146

Start: 1089390

End: 1090046

Strand: Direct

Name: thiED [H]

Synonym: Chy400_0862

Alternate gene names: 222524146

Gene position: 1089390-1090046 (Clockwise)

Preceding gene: 222524145

Following gene: 222524151

Centisome position: 20.68

GC content: 57.23

Gene sequence:

>657_bases
ATGACACGCTTTACCGACACCCTTTGGTCATCAATTACCGACATCTATCAGGCCATTATCTATCACCCGTTCAACGAGGA
GCTGGCGCGGGGAATTTTGCCGCGCGAGAAGTTCGCCTTCTACATGCAGCAGGATGCGCTGTACCTGGCCGATTTTGCCC
GCGCACTGGCGATAATGGCCGGACGGGCACCTGATGAAGCGGCGATTATTCAGTTTGTGCGCTTTGCTGAAGGGGTGGCG
GTGGTTGAGCGCGCTCTCCATCACACCTATTTTCGTGAATTTGGGATTGAGACGCCAACGCGGCAGCCGGCTCCGGCCTG
TTTTACCTACACCAACTTCTTGCTGGCAACCGCGGCCTGTCGCAGTTACGAAGAGGGGATGGCGGCGCTGTTGCCGTGCT
TCTGGATTTATCGTGAGGTGGGACACGACATCTATCGGCGTGCAGCGCCCAACAATCCCTACCAGAAGTGGATTGACACC
TACGCCGGCCAGGAGTTTGATGAGGTGGTGACGCAGGCAATTGCGCTGACCGACACCATCGCTGAACAGGCATCGGCGAC
GCAGCAACAACGTATGCGGGACGCCTTCATCTATTCGGCTCGCCTGGAGTGGATGTTCTGGGATAGTGCGTATCGGCTGG
AGCAGTGGTTACCCTGA

Upstream 100 bases:

>100_bases
CACGATGTTCGGCCAGTCTCCTGAGTTCGTTCTCGCTTCCCCTCTCAGTCGTCTGCTCTCTGCGGATACGGTATCCGGCT
TTTGGACGCAGGGAGGATGT

Downstream 100 bases:

>100_bases
TGATGCTCTCATCATCCCGGCATCTCTTGGGATGTCAAGGCGGACAGAACCTGCGAGTGCGTTCGCGCGATATATCCTGG
CGATGCGCTGAACGCCCTCA

Product: transcriptional activator, TenA family

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTRFTDTLWSSITDIYQAIIYHPFNEELARGILPREKFAFYMQQDALYLADFARALAIMAGRAPDEAAIIQFVRFAEGVA
VVERALHHTYFREFGIETPTRQPAPACFTYTNFLLATAACRSYEEGMAALLPCFWIYREVGHDIYRRAAPNNPYQKWIDT
YAGQEFDEVVTQAIALTDTIAEQASATQQQRMRDAFIYSARLEWMFWDSAYRLEQWLP

Sequences:

>Translated_218_residues
MTRFTDTLWSSITDIYQAIIYHPFNEELARGILPREKFAFYMQQDALYLADFARALAIMAGRAPDEAAIIQFVRFAEGVA
VVERALHHTYFREFGIETPTRQPAPACFTYTNFLLATAACRSYEEGMAALLPCFWIYREVGHDIYRRAAPNNPYQKWIDT
YAGQEFDEVVTQAIALTDTIAEQASATQQQRMRDAFIYSARLEWMFWDSAYRLEQWLP
>Mature_217_residues
TRFTDTLWSSITDIYQAIIYHPFNEELARGILPREKFAFYMQQDALYLADFARALAIMAGRAPDEAAIIQFVRFAEGVAV
VERALHHTYFREFGIETPTRQPAPACFTYTNFLLATAACRSYEEGMAALLPCFWIYREVGHDIYRRAAPNNPYQKWIDTY
AGQEFDEVVTQAIALTDTIAEQASATQQQRMRDAFIYSARLEWMFWDSAYRLEQWLP

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=208, Percent_Identity=27.4038461538462, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325378, Length=206, Percent_Identity=27.1844660194175, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324997, Length=207, Percent_Identity=27.536231884058, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 25319; Mature: 25188

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFTDTLWSSITDIYQAIIYHPFNEELARGILPREKFAFYMQQDALYLADFARALAIMA
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAPDEAAIIQFVRFAEGVAVVERALHHTYFREFGIETPTRQPAPACFTYTNFLLATAAC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
RSYEEGMAALLPCFWIYREVGHDIYRRAAPNNPYQKWIDTYAGQEFDEVVTQAIALTDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AEQASATQQQRMRDAFIYSARLEWMFWDSAYRLEQWLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
TRFTDTLWSSITDIYQAIIYHPFNEELARGILPREKFAFYMQQDALYLADFARALAIMA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAPDEAAIIQFVRFAEGVAVVERALHHTYFREFGIETPTRQPAPACFTYTNFLLATAAC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
RSYEEGMAALLPCFWIYREVGHDIYRRAAPNNPYQKWIDTYAGQEFDEVVTQAIALTDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AEQASATQQQRMRDAFIYSARLEWMFWDSAYRLEQWLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]