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The map label for this gene is fruB(HI) [H]

Identifier: 222524151

GI number: 222524151

Start: 1096455

End: 1098917

Strand: Direct

Name: fruB(HI) [H]

Synonym: Chy400_0867

Alternate gene names: 222524151

Gene position: 1096455-1098917 (Clockwise)

Preceding gene: 222524146

Following gene: 222524152

Centisome position: 20.81

GC content: 62.08

Gene sequence:

>2463_bases
ATGGTAAAGCTAACCGCCGCGAGTGTGCGCATCGGCGCGACCGCGACCTCGAAAGCAGAGGCGATTCGTCAGGTTGGGCA
GGTGCTGGTCGAGGCCGGCCATATTCAACCTGCGTATATCGAGAGTATGCTGGCCCGTGAAGCGCTGGCCAATACCTTCC
TCGGTAATGGAATTGCTATTCCGCACGGCAAACCGGAAGACCGCGACCTCATCCTCGAAACCGGCATTGCTGTCTTGCAA
ATTCCCAACGGCGTCACGTGGAATGCGGGTGAGACGGCTCGTTTGATTGTCGGCATTGCCGCCCGTTCCGACGAGCATAT
TGACATCTTACGCCGCCTGACCCGTGTGCTCGGTGATGCGGCGCTGGTCGAACGGTTATCCCAGACTCGTGATCCTGCCG
ATATTGTCGAAGCGCTGACCGGTAGCCGTCCCCTGCCCGCTTCCCCATCCCCCGGTGACTACGATCACGCGATTCAGGTG
GTGATTCACAATCCAACCGGCCTGCACGCCCGCCCGGCAACCGCCTTTGTCGAAACGGCCAGACGCTTTCAGGCCGCGGT
GCGCGTCCGCTATGGCGATGCGGTGGCCGATGGCAAGAGCCTCTTGAGTCTGTTGCAATTGGGGGTGAGTAGTGGGGCCA
GCGTTACCATTTCGGCTCAGGGTACCGACGCTCCGGCGGCCTTAGCCGCGCTCAAGGCCCTGGTTGAGAGTGGTATGGGC
GAGGAACCGGTTGCCGAAGACACGGCCAATCGTCCGAAAGTGCCCTACCGCGGGTGGGTGCCACAGCACGTTGCTACGAC
CATTAATGGCATCGCTGCCGCCGAGGGGCTGGCGGTTGGGCCGATCCGCCACTACCGCCGGGCACCGCTGGTTGTCAGCG
ATACTCCCGGTGACCGGATGAGCGAAGCCACCGCTCTCGAACGGGCCATTGCCGAGGCGCGCAGCGAGCTTGAGCTGGTG
GCCGGTGAAGTCAGCCGTCGGCTTGGTTCATCTCGCGCCGCCATTTTCCGTGCCCATGCCGAATTGCTGACCGACCCGGC
GCTGATGCGTGAAACTGTCAGCCGGATTTTCGACGGCCACAGCGCGGCCTGGGCCTGGCAACAGACCATCGCGGCCCGTG
TTGCTCAGCTTGAAAAGCTTGATGACCCGGTACTGGCCGGGCGCGCTGTCGATCTGAGCGATGTCGGCCAGCGGGTGTTG
CGCCACTTGCTCGGCCTGGGTGAGATGCCGTTCATCAGTCTGGCCGAACCGGCAATTCTGGTAGCCGATGACCTGACCCC
TTCTGACACGGCCACGCTCGATCCCGACAGTGTGCTGGGGCTTTGTACAGCGCTCGGTGGCCCAACGTCGCACACCGCGA
TTATCGCCCGTTCACTCGGCCTGCCGGCCATCGTTGCCGCCGGTACTGCCGTGCTCGATATTGAAGACGGTACGCCGGCA
ATTCTCGATGGCTACAGTGGCCGGCTCTATCTTCGACCATCAGCCGCCGATGTTGAAGCAGCGCGTGCCTTGCGCGCCGG
TCTCGATCAGGAACAGGCGGTGGCATTCGCTACCCGTCATCAGCCGGCCATCACCCGCGATGGTGTCCATATCGAAGTTG
CTGCCAACGTCAACCGGGTAGCTGATGTTGTGCGTGCCCTTGAGAACGGTGCCGACGGGGTTGGCTTAATGCGTACCGAG
TTTCTGTTCCTCGAACGCGACAGTGCTCCCGACGAAGAGGAACAGTATCAGGCGTACCGGGCGATGGTTGAGGCGATGAA
TGGGCGTCCGCTGATCATTCGCACCCTCGACATTGGGGGCGATAAAGAGGCACCCTATCTCAATATTGCCCGCGAAGACA
ATTCGTTCCTGGGTATTCGTGGCTTACGGCTCTGCCTGCGCCGACCCGATCTGTTCGAGCCGCAATTACGCGCCATCTAC
CGCGCCGCTATGCACGGCCCGCTCAAAATGATGTTTCCGATGGTCGCTACGCTGGAAGAGGTGCAACAGGCCAAAGCGAT
TGCCGAGCGCATCCGTGCCGAACTCAATGCGCCACCGGTCGAGATCGGGATTATGGTCGAGGTACCATCGGCAGCCATGC
TGGCCGACGTGCTGGCGCAAGAGGTCGATTTCTTCTCCATCGGCACCAACGATTTGACGCAGTACGTCCTGGCGATGGAT
CGCCTGCATCCAGACCTGGCCCGCCAGGCCGATAGTCTGCACCCGGCTGTGCTCCGCACAATTGCCCGCACCGTCGAAGG
CGCAGCCAGTGCCGGACGCTGGGTCGGTGTCTGTGGTGGTATTGCCAGTGATCCGCTCGGCGCAGCGATTCTGGTCGGTT
TAGGCGTCCAAGAGCTGAGCGTCAGTATTCCCAGTGTCGCGACCATCAAAGCGCACCTGCGCGGTCTGAGTGCCGCCGAT
CTGCGTGATCTGGCGCGACGAGCACTGGCGTGTCGTAATGCAGCGGAGGTGCGCGCACTATGA

Upstream 100 bases:

>100_bases
ACCCTCTGCCCTCCTTGCGCGACAGGTTGTCGATTTACCGCAACGAAGCGAAGAACAGGTTCGGGCAGCCAGGCAGGCTG
CTGCACAGGGGAGTGCTCTT

Downstream 100 bases:

>100_bases
ACATCGCTACCATAACCCTCAATCCGGCCATTGATCAGACGGTCTTCGTCGATCATTTTCAATTGAACACCGTGAACCGG
GCACGCGCTATGCAACGCGA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 820; Mature: 820

Protein sequence:

>820_residues
MVKLTAASVRIGATATSKAEAIRQVGQVLVEAGHIQPAYIESMLAREALANTFLGNGIAIPHGKPEDRDLILETGIAVLQ
IPNGVTWNAGETARLIVGIAARSDEHIDILRRLTRVLGDAALVERLSQTRDPADIVEALTGSRPLPASPSPGDYDHAIQV
VIHNPTGLHARPATAFVETARRFQAAVRVRYGDAVADGKSLLSLLQLGVSSGASVTISAQGTDAPAALAALKALVESGMG
EEPVAEDTANRPKVPYRGWVPQHVATTINGIAAAEGLAVGPIRHYRRAPLVVSDTPGDRMSEATALERAIAEARSELELV
AGEVSRRLGSSRAAIFRAHAELLTDPALMRETVSRIFDGHSAAWAWQQTIAARVAQLEKLDDPVLAGRAVDLSDVGQRVL
RHLLGLGEMPFISLAEPAILVADDLTPSDTATLDPDSVLGLCTALGGPTSHTAIIARSLGLPAIVAAGTAVLDIEDGTPA
ILDGYSGRLYLRPSAADVEAARALRAGLDQEQAVAFATRHQPAITRDGVHIEVAANVNRVADVVRALENGADGVGLMRTE
FLFLERDSAPDEEEQYQAYRAMVEAMNGRPLIIRTLDIGGDKEAPYLNIAREDNSFLGIRGLRLCLRRPDLFEPQLRAIY
RAAMHGPLKMMFPMVATLEEVQQAKAIAERIRAELNAPPVEIGIMVEVPSAAMLADVLAQEVDFFSIGTNDLTQYVLAMD
RLHPDLARQADSLHPAVLRTIARTVEGAASAGRWVGVCGGIASDPLGAAILVGLGVQELSVSIPSVATIKAHLRGLSAAD
LRDLARRALACRNAAEVRAL

Sequences:

>Translated_820_residues
MVKLTAASVRIGATATSKAEAIRQVGQVLVEAGHIQPAYIESMLAREALANTFLGNGIAIPHGKPEDRDLILETGIAVLQ
IPNGVTWNAGETARLIVGIAARSDEHIDILRRLTRVLGDAALVERLSQTRDPADIVEALTGSRPLPASPSPGDYDHAIQV
VIHNPTGLHARPATAFVETARRFQAAVRVRYGDAVADGKSLLSLLQLGVSSGASVTISAQGTDAPAALAALKALVESGMG
EEPVAEDTANRPKVPYRGWVPQHVATTINGIAAAEGLAVGPIRHYRRAPLVVSDTPGDRMSEATALERAIAEARSELELV
AGEVSRRLGSSRAAIFRAHAELLTDPALMRETVSRIFDGHSAAWAWQQTIAARVAQLEKLDDPVLAGRAVDLSDVGQRVL
RHLLGLGEMPFISLAEPAILVADDLTPSDTATLDPDSVLGLCTALGGPTSHTAIIARSLGLPAIVAAGTAVLDIEDGTPA
ILDGYSGRLYLRPSAADVEAARALRAGLDQEQAVAFATRHQPAITRDGVHIEVAANVNRVADVVRALENGADGVGLMRTE
FLFLERDSAPDEEEQYQAYRAMVEAMNGRPLIIRTLDIGGDKEAPYLNIAREDNSFLGIRGLRLCLRRPDLFEPQLRAIY
RAAMHGPLKMMFPMVATLEEVQQAKAIAERIRAELNAPPVEIGIMVEVPSAAMLADVLAQEVDFFSIGTNDLTQYVLAMD
RLHPDLARQADSLHPAVLRTIARTVEGAASAGRWVGVCGGIASDPLGAAILVGLGVQELSVSIPSVATIKAHLRGLSAAD
LRDLARRALACRNAAEVRAL
>Mature_820_residues
MVKLTAASVRIGATATSKAEAIRQVGQVLVEAGHIQPAYIESMLAREALANTFLGNGIAIPHGKPEDRDLILETGIAVLQ
IPNGVTWNAGETARLIVGIAARSDEHIDILRRLTRVLGDAALVERLSQTRDPADIVEALTGSRPLPASPSPGDYDHAIQV
VIHNPTGLHARPATAFVETARRFQAAVRVRYGDAVADGKSLLSLLQLGVSSGASVTISAQGTDAPAALAALKALVESGMG
EEPVAEDTANRPKVPYRGWVPQHVATTINGIAAAEGLAVGPIRHYRRAPLVVSDTPGDRMSEATALERAIAEARSELELV
AGEVSRRLGSSRAAIFRAHAELLTDPALMRETVSRIFDGHSAAWAWQQTIAARVAQLEKLDDPVLAGRAVDLSDVGQRVL
RHLLGLGEMPFISLAEPAILVADDLTPSDTATLDPDSVLGLCTALGGPTSHTAIIARSLGLPAIVAAGTAVLDIEDGTPA
ILDGYSGRLYLRPSAADVEAARALRAGLDQEQAVAFATRHQPAITRDGVHIEVAANVNRVADVVRALENGADGVGLMRTE
FLFLERDSAPDEEEQYQAYRAMVEAMNGRPLIIRTLDIGGDKEAPYLNIAREDNSFLGIRGLRLCLRRPDLFEPQLRAIY
RAAMHGPLKMMFPMVATLEEVQQAKAIAERIRAELNAPPVEIGIMVEVPSAAMLADVLAQEVDFFSIGTNDLTQYVLAMD
RLHPDLARQADSLHPAVLRTIARTVEGAASAGRWVGVCGGIASDPLGAAILVGLGVQELSVSIPSVATIKAHLRGLSAAD
LRDLARRALACRNAAEVRAL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=565, Percent_Identity=40.5309734513274, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1788726, Length=685, Percent_Identity=34.3065693430657, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI48994992, Length=685, Percent_Identity=33.4306569343066, Blast_Score=323, Evalue=3e-89,
Organism=Escherichia coli, GI1789193, Length=567, Percent_Identity=36.331569664903, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1788494, Length=143, Percent_Identity=46.1538461538462, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=42.0689655172414, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI226510935, Length=350, Percent_Identity=30.2857142857143, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1787994, Length=445, Percent_Identity=27.1910112359551, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 87026; Mature: 87026

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00369 PTS_HPR_HIS ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLTAASVRIGATATSKAEAIRQVGQVLVEAGHIQPAYIESMLAREALANTFLGNGIAI
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEC
PHGKPEDRDLILETGIAVLQIPNGVTWNAGETARLIVGIAARSDEHIDILRRLTRVLGDA
CCCCCCCCCHHHHCCEEEEECCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHCHH
ALVERLSQTRDPADIVEALTGSRPLPASPSPGDYDHAIQVVIHNPTGLHARPATAFVETA
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
RRFQAAVRVRYGDAVADGKSLLSLLQLGVSSGASVTISAQGTDAPAALAALKALVESGMG
HHHHHHHHEEECCHHHCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
EEPVAEDTANRPKVPYRGWVPQHVATTINGIAAAEGLAVGPIRHYRRAPLVVSDTPGDRM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEEECCCCCHH
SEATALERAIAEARSELELVAGEVSRRLGSSRAAIFRAHAELLTDPALMRETVSRIFDGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
SAAWAWQQTIAARVAQLEKLDDPVLAGRAVDLSDVGQRVLRHLLGLGEMPFISLAEPAIL
CHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCEE
VADDLTPSDTATLDPDSVLGLCTALGGPTSHTAIIARSLGLPAIVAAGTAVLDIEDGTPA
EECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCEEEEEECCCCCC
ILDGYSGRLYLRPSAADVEAARALRAGLDQEQAVAFATRHQPAITRDGVHIEVAANVNRV
EECCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEECCCEEEEEECCHHHH
ADVVRALENGADGVGLMRTEFLFLERDSAPDEEEQYQAYRAMVEAMNGRPLIIRTLDIGG
HHHHHHHHCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
DKEAPYLNIAREDNSFLGIRGLRLCLRRPDLFEPQLRAIYRAAMHGPLKMMFPMVATLEE
CCCCCEEEEEECCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VQQAKAIAERIRAELNAPPVEIGIMVEVPSAAMLADVLAQEVDFFSIGTNDLTQYVLAMD
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEECCHHHHHHHHHHHH
RLHPDLARQADSLHPAVLRTIARTVEGAASAGRWVGVCGGIASDPLGAAILVGLGVQELS
HHCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCHHHHH
VSIPSVATIKAHLRGLSAADLRDLARRALACRNAAEVRAL
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MVKLTAASVRIGATATSKAEAIRQVGQVLVEAGHIQPAYIESMLAREALANTFLGNGIAI
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEC
PHGKPEDRDLILETGIAVLQIPNGVTWNAGETARLIVGIAARSDEHIDILRRLTRVLGDA
CCCCCCCCCHHHHCCEEEEECCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHCHH
ALVERLSQTRDPADIVEALTGSRPLPASPSPGDYDHAIQVVIHNPTGLHARPATAFVETA
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
RRFQAAVRVRYGDAVADGKSLLSLLQLGVSSGASVTISAQGTDAPAALAALKALVESGMG
HHHHHHHHEEECCHHHCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
EEPVAEDTANRPKVPYRGWVPQHVATTINGIAAAEGLAVGPIRHYRRAPLVVSDTPGDRM
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEEECCCCCHH
SEATALERAIAEARSELELVAGEVSRRLGSSRAAIFRAHAELLTDPALMRETVSRIFDGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
SAAWAWQQTIAARVAQLEKLDDPVLAGRAVDLSDVGQRVLRHLLGLGEMPFISLAEPAIL
CHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCEE
VADDLTPSDTATLDPDSVLGLCTALGGPTSHTAIIARSLGLPAIVAAGTAVLDIEDGTPA
EECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCEEEEEECCCCCC
ILDGYSGRLYLRPSAADVEAARALRAGLDQEQAVAFATRHQPAITRDGVHIEVAANVNRV
EECCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEECCCEEEEEECCHHHH
ADVVRALENGADGVGLMRTEFLFLERDSAPDEEEQYQAYRAMVEAMNGRPLIIRTLDIGG
HHHHHHHHCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
DKEAPYLNIAREDNSFLGIRGLRLCLRRPDLFEPQLRAIYRAAMHGPLKMMFPMVATLEE
CCCCCEEEEEECCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
VQQAKAIAERIRAELNAPPVEIGIMVEVPSAAMLADVLAQEVDFFSIGTNDLTQYVLAMD
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEECCHHHHHHHHHHHH
RLHPDLARQADSLHPAVLRTIARTVEGAASAGRWVGVCGGIASDPLGAAILVGLGVQELS
HHCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCHHHHH
VSIPSVATIKAHLRGLSAADLRDLARRALACRNAAEVRAL
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193161 [H]