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The map label for this gene is thiD [H]

Identifier: 222152087

GI number: 222152087

Start: 1978654

End: 1979484

Strand: Direct

Name: thiD [H]

Synonym: MCCL_1844

Alternate gene names: 222152087

Gene position: 1978654-1979484 (Clockwise)

Preceding gene: 222152082

Following gene: 222152088

Centisome position: 94.12

GC content: 38.87

Gene sequence:

>831_bases
ATGAGCTTAAAAAAGATTTTAACAATTGCTGGTTCTGATACAAGTGCTGGAGCTGGTATGCAAGCTGACTTAAAAACTTT
CCAGGAATATAACACATACGGTATGGTGGCTTTAACCGCTGTAGTCGCAATGGATCCAGAAACTTGGGCCCATAATGTTA
CACCGCTGCCAATCGAATTATTAGATAAACAAATTAAAACTGCATTATCTATCGGCCCTGATGCCATTAAAACAGGTATG
TTAGGAACTGAAGAAATCATCCAGCGTGCGGCCAAAGCTTTTGAAGATTCTGATGCTGCGTATTTCGTAGTGGACCCTGT
TATGGTATGCAAAGGGGAAGATGAAGTGCTTAATCCTGGCAACACGGATGCTATGATTAAATATTTATTACCACTTGCTA
CAATTGTGACACCGAACTTGTTCGAAGCAGGTCAATTATCTGGTTTAGGTAAACTAACAACAATGGAGCAAATGAAAGAA
GCTGCTGAAATCATTCACCAACGTGGTGCAAAACACGTTATCATCAAAGGCGGAAAAGCTTTAGAATCTGATGAAGCATT
AGATTTATATTATGATGGCGATACTTATACATTACTGACAACTGATAAATTCCAATCATCATATAATCATGGTGCAGGTT
GTACATTTGCTGCTGCTACAACAGCAAACTTAGCATTAGGTAAATCTCCACGTGAAGCGGTAATTGCTGCTAAAGGATTT
GTCGCATCAGCAATTAAACACGGATGGAAAATGAACGAATTTGTAGGACCAGTACAGCATGGTGCTTACAATAATGTTGA
GAAGATTGAAGTAACAACGAAAGAAGTGTAA

Upstream 100 bases:

>100_bases
TCCATATTATCCACCTCATATATATTATAAATATATCATTTTGAATTAAACGTATTCATGATATGATATTAACAAACAAA
CAAGAATAGGGGAAATGAAA

Downstream 100 bases:

>100_bases
TAAAAAGGTTGTGACATGAGTAATATTACGAAAAGATGGCAGGAAATGTTCTTTGTTTCCAGTCATCTTTTTTGATTTAA
CTCGTGTCTGCTCGTGAAAC

Product: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSLKKILTIAGSDTSAGAGMQADLKTFQEYNTYGMVALTAVVAMDPETWAHNVTPLPIELLDKQIKTALSIGPDAIKTGM
LGTEEIIQRAAKAFEDSDAAYFVVDPVMVCKGEDEVLNPGNTDAMIKYLLPLATIVTPNLFEAGQLSGLGKLTTMEQMKE
AAEIIHQRGAKHVIIKGGKALESDEALDLYYDGDTYTLLTTDKFQSSYNHGAGCTFAAATTANLALGKSPREAVIAAKGF
VASAIKHGWKMNEFVGPVQHGAYNNVEKIEVTTKEV

Sequences:

>Translated_276_residues
MSLKKILTIAGSDTSAGAGMQADLKTFQEYNTYGMVALTAVVAMDPETWAHNVTPLPIELLDKQIKTALSIGPDAIKTGM
LGTEEIIQRAAKAFEDSDAAYFVVDPVMVCKGEDEVLNPGNTDAMIKYLLPLATIVTPNLFEAGQLSGLGKLTTMEQMKE
AAEIIHQRGAKHVIIKGGKALESDEALDLYYDGDTYTLLTTDKFQSSYNHGAGCTFAAATTANLALGKSPREAVIAAKGF
VASAIKHGWKMNEFVGPVQHGAYNNVEKIEVTTKEV
>Mature_275_residues
SLKKILTIAGSDTSAGAGMQADLKTFQEYNTYGMVALTAVVAMDPETWAHNVTPLPIELLDKQIKTALSIGPDAIKTGML
GTEEIIQRAAKAFEDSDAAYFVVDPVMVCKGEDEVLNPGNTDAMIKYLLPLATIVTPNLFEAGQLSGLGKLTTMEQMKEA
AEIIHQRGAKHVIIKGGKALESDEALDLYYDGDTYTLLTTDKFQSSYNHGAGCTFAAATTANLALGKSPREAVIAAKGFV
ASAIKHGWKMNEFVGPVQHGAYNNVEKIEVTTKEV

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=30.7984790874525, Blast_Score=117, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=29.7101449275362, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324997, Length=280, Percent_Identity=30, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=27.2058823529412, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 29591; Mature: 29460

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKKILTIAGSDTSAGAGMQADLKTFQEYNTYGMVALTAVVAMDPETWAHNVTPLPIEL
CCHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCHHH
LDKQIKTALSIGPDAIKTGMLGTEEIIQRAAKAFEDSDAAYFVVDPVMVCKGEDEVLNPG
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCC
NTDAMIKYLLPLATIVTPNLFEAGQLSGLGKLTTMEQMKEAAEIIHQRGAKHVIIKGGKA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LESDEALDLYYDGDTYTLLTTDKFQSSYNHGAGCTFAAATTANLALGKSPREAVIAAKGF
CCCCCCEEEEECCCEEEEEEEHHHHHCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHH
VASAIKHGWKMNEFVGPVQHGAYNNVEKIEVTTKEV
HHHHHHCCCCHHHHCCCHHCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
SLKKILTIAGSDTSAGAGMQADLKTFQEYNTYGMVALTAVVAMDPETWAHNVTPLPIEL
CHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCHHH
LDKQIKTALSIGPDAIKTGMLGTEEIIQRAAKAFEDSDAAYFVVDPVMVCKGEDEVLNPG
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCC
NTDAMIKYLLPLATIVTPNLFEAGQLSGLGKLTTMEQMKEAAEIIHQRGAKHVIIKGGKA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LESDEALDLYYDGDTYTLLTTDKFQSSYNHGAGCTFAAATTANLALGKSPREAVIAAKGF
CCCCCCEEEEECCCEEEEEEEHHHHHCCCCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHH
VASAIKHGWKMNEFVGPVQHGAYNNVEKIEVTTKEV
HHHHHHCCCCHHHHCCCHHCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA