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The map label for this gene is ispH

Identifier: 222151456

GI number: 222151456

Start: 1312249

End: 1313217

Strand: Direct

Name: ispH

Synonym: MCCL_1209

Alternate gene names: 222151456

Gene position: 1312249-1313217 (Clockwise)

Preceding gene: 222151450

Following gene: 222151464

Centisome position: 62.42

GC content: 39.63

Gene sequence:

>969_bases
ATGGAAATCATTAAAATTACACCTCGTGGCTATTGTTATGGCGTAGTTGATGCAATGGTCATCGCACGTAACGCATCCTT
AGACAAGACCCTACCACGTCCTATTTATATACTTGGTATGATCGTTCATAATAAACATGTCACAGATGCATTTGAAAGTG
ACGGCATTATTACATTGGATGGTCCTAACCGACTTGAAATCTTAGAACAGATTGAAACTGGAACAGTTATCTTCACAGCA
CATGGTGTAAGTCCAGAAGTAAAACGTCGTGCAAAAGAAAAAGGCCTTGTCTGTATCGATGCCACTTGCCCAGATGTTGA
AAATACACATACATTGATTCGTCGTAAGAAAGCTGACGGCTATCATGTCATCTATATCGGTAAGAAAGGTCACCCTGAAC
CTGAAGGAGCAGTGGGTGTTGCTCCAGATATCGTTCACCTTGTAGAAAACAAGCAGGATATCGAACAATTACCAGAATCA
TTAAATGATCAGAAACTCATTGTAACAAACCAAACAACAATGAGTCAGTGGGATGTTAAACATCTGATGGAAGACTTAGA
AGAGAAGTTTCCGCACATTGAAGTGCATAAAGAAATATGTCTTGCAACACAAGTAAGACAGGAAGCCGTTGCTAATCAGG
CTCCAAGTGCGGATGTTCTGATCGTTGTCGGTGACCCTAAAAGTAATAACTCTAACAGACTCGCACAAGTTTCAAAAGAA
ATCGCACATACTGAAGCCTATCGTATTTCAGATATCAGTGAATTAAAGCTAGAGTGGCTTGAGGGAAAATCAACGATTGC
AGTTACAGCAGGAGCTTCCACACCTACACCGATTGTTAAAGAAGTGATCGACTATATTAAAGGCTATGATCCACTAAATA
TTACTCCTATACCTGAAACGTCTGGTGTACCAGTTGACAAGATTTTACCTAAAATTAAAAACGCAGCCCCTGTCAAAATA
TTAGATTAA

Upstream 100 bases:

>100_bases
TATTATATAGGAAACATTACTAAAAAGAAAGTAAACATTTTTAAAACCCTATTATAAATGGTATGATGATAGTACTGATT
CATGAATTGGAGGCACAATA

Downstream 100 bases:

>100_bases
AAAAAGTGCCATCGTCAGTAGACGATGGCACTTTTGTTTATATTTCATTAAACGGATTAGTATTTGTCGTTGATGCGACA
ACTGTAATTTCAGGCATCAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MEIIKITPRGYCYGVVDAMVIARNASLDKTLPRPIYILGMIVHNKHVTDAFESDGIITLDGPNRLEILEQIETGTVIFTA
HGVSPEVKRRAKEKGLVCIDATCPDVENTHTLIRRKKADGYHVIYIGKKGHPEPEGAVGVAPDIVHLVENKQDIEQLPES
LNDQKLIVTNQTTMSQWDVKHLMEDLEEKFPHIEVHKEICLATQVRQEAVANQAPSADVLIVVGDPKSNNSNRLAQVSKE
IAHTEAYRISDISELKLEWLEGKSTIAVTAGASTPTPIVKEVIDYIKGYDPLNITPIPETSGVPVDKILPKIKNAAPVKI
LD

Sequences:

>Translated_322_residues
MEIIKITPRGYCYGVVDAMVIARNASLDKTLPRPIYILGMIVHNKHVTDAFESDGIITLDGPNRLEILEQIETGTVIFTA
HGVSPEVKRRAKEKGLVCIDATCPDVENTHTLIRRKKADGYHVIYIGKKGHPEPEGAVGVAPDIVHLVENKQDIEQLPES
LNDQKLIVTNQTTMSQWDVKHLMEDLEEKFPHIEVHKEICLATQVRQEAVANQAPSADVLIVVGDPKSNNSNRLAQVSKE
IAHTEAYRISDISELKLEWLEGKSTIAVTAGASTPTPIVKEVIDYIKGYDPLNITPIPETSGVPVDKILPKIKNAAPVKI
LD
>Mature_322_residues
MEIIKITPRGYCYGVVDAMVIARNASLDKTLPRPIYILGMIVHNKHVTDAFESDGIITLDGPNRLEILEQIETGTVIFTA
HGVSPEVKRRAKEKGLVCIDATCPDVENTHTLIRRKKADGYHVIYIGKKGHPEPEGAVGVAPDIVHLVENKQDIEQLPES
LNDQKLIVTNQTTMSQWDVKHLMEDLEEKFPHIEVHKEICLATQVRQEAVANQAPSADVLIVVGDPKSNNSNRLAQVSKE
IAHTEAYRISDISELKLEWLEGKSTIAVTAGASTPTPIVKEVIDYIKGYDPLNITPIPETSGVPVDKILPKIKNAAPVKI
LD

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=292, Percent_Identity=36.6438356164384, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_MACCJ (B9E6U8)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560612.1
- GeneID:   7389162
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_1209
- OMA:   WGAPVYV
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 35528; Mature: 35528

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIKITPRGYCYGVVDAMVIARNASLDKTLPRPIYILGMIVHNKHVTDAFESDGIITLD
CCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCEEEEC
GPNRLEILEQIETGTVIFTAHGVSPEVKRRAKEKGLVCIDATCPDVENTHTLIRRKKADG
CCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCC
YHVIYIGKKGHPEPEGAVGVAPDIVHLVENKQDIEQLPESLNDQKLIVTNQTTMSQWDVK
EEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
HLMEDLEEKFPHIEVHKEICLATQVRQEAVANQAPSADVLIVVGDPKSNNSNRLAQVSKE
HHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHH
IAHTEAYRISDISELKLEWLEGKSTIAVTAGASTPTPIVKEVIDYIKGYDPLNITPIPET
HHHHHHHCCCCHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEECCCC
SGVPVDKILPKIKNAAPVKILD
CCCCHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MEIIKITPRGYCYGVVDAMVIARNASLDKTLPRPIYILGMIVHNKHVTDAFESDGIITLD
CCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCEEEEC
GPNRLEILEQIETGTVIFTAHGVSPEVKRRAKEKGLVCIDATCPDVENTHTLIRRKKADG
CCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCC
YHVIYIGKKGHPEPEGAVGVAPDIVHLVENKQDIEQLPESLNDQKLIVTNQTTMSQWDVK
EEEEEECCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
HLMEDLEEKFPHIEVHKEICLATQVRQEAVANQAPSADVLIVVGDPKSNNSNRLAQVSKE
HHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHH
IAHTEAYRISDISELKLEWLEGKSTIAVTAGASTPTPIVKEVIDYIKGYDPLNITPIPET
HHHHHHHCCCCHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEECCCC
SGVPVDKILPKIKNAAPVKILD
CCCCHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA