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The map label for this gene is glyS

Identifier: 222151464

GI number: 222151464

Start: 1320159

End: 1321547

Strand: Direct

Name: glyS

Synonym: MCCL_1217

Alternate gene names: 222151464

Gene position: 1320159-1321547 (Clockwise)

Preceding gene: 222151456

Following gene: 222151510

Centisome position: 62.8

GC content: 39.02

Gene sequence:

>1389_bases
ATGGCAGAAAAAAACATGGAAACAATTGTTAATCTTGCAAAGCACAGAGGATTTGTCTTTCCTGGTAGTGAAATCTACGG
CGGACTTGCAAACACTTGGGACTACGGTCCGTTAGGTGTGGAGCTTAAAAACAATGTAAAACGTGCATGGTGGCAAAAGT
TCGTTCAGCAATCACCTTATAATGTAGGTCTCGATGCTGCGATTCTGATGAACCCAAAAACTTGGGAAGCAAGTGGTCAC
TTATCTAACTTTAACGACCCTATGATCGATAACAAAGATTCTAAGATTCGTTACCGTGCAGATAAATTAATTGAAGATTA
TATGCACGCACAAGGTGATGAACACTTTATCGCTGACGGTATGAGCTTTGATGAAATGAAACAGTTCATTGATGAAAAAG
GTATCGTTTGTCCTGTTTCAGGTACAGCAAACTGGACTGAAATTCGTCAATTCAATTTAATGTTCAAAACATTCCAGGGT
GTTACTGAATCATCTACAAATGAAATTTTCCTGCGTCCTGAAACAGCACAAGGTATATTTGTAAACTATAAGAATGTTCA
ACGCTCAATGCGTAAGAAACTCCCATTCGGTATTGCACAAGTAGGTAAATCATTCCGTAATGAAATCACACCAGGTAACT
TTATCTTCAGAACGCGAGAATTCGAACAGATGGAATTAGAATTCTTCTGTAAACCAGGGACAGAAATTGAATGGCAGGAA
TACTGGAAGAATTTTGCCGCGAAATGGCTGAAAGATTTAGGCATTAAAGAAGAGAACACAAAACTTCGTGACCATGATGA
AGACGAATTATCTCACTACTCTAATGCCACAACAGATATCGAATATAAATTTCCATTTGGCTGGGGTGAATTATGGGGTA
TTGCAAGCCGTACAGACTTCGACTTAAAAGCGCATATGGCAGCAAGCGGAGATGACTTTAGCTATCATGATCAGGAAACG
AACGAAAAATATGTACCTTACTGTATCGAACCATCACTTGGTGCAGATCGTGTGACATTAGCATTTTTATGTGATGCTTA
TGAAGAAGAAACACTTGAAGGCGGAGATTCTCGTACAGTGATGCGTTTCCACCCGGCACTCGCACCTTACAAAGCTGCAA
TCCTGCCGTTAAGTAAAAAGCTGTCACCAGATGCGATGAAAGTTTATGAAGCATTAAGTGCTGACTTTGCTATTGATTTT
GATGAAAGTCAGTCAATTGGTAAACGTTACAGAAGACAAGACGAAATCGGTACACCATTCTGTATCACATTTGACTTCGA
TTCGCTAGAAGATAATCAAGTTACTGTTCGTGACCGTGACACGATGGAACAAGTACGTATGCCAATTAGCGAAGTAAAAG
CATTTTTAGATGAAAAAATTAAATTTTAA

Upstream 100 bases:

>100_bases
AAACACTAATTTGGGTGGAACCGCGGGTTTATATACTCGTCCCAAGGCTTAATTGCCTTGGGACTTTTTTGTTTACTCTA
AATTAATAGGAGGTCATATT

Downstream 100 bases:

>100_bases
AATGATAGAAGCGGGTTCATTTTCATGAACTCGCTTCTTTATTTATAATTATCCAGTCTTCTCATCTGCTCCAGAATCTT
CCTCGACTTGATAAAGACAC

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAEKNMETIVNLAKHRGFVFPGSEIYGGLANTWDYGPLGVELKNNVKRAWWQKFVQQSPYNVGLDAAILMNPKTWEASGH
LSNFNDPMIDNKDSKIRYRADKLIEDYMHAQGDEHFIADGMSFDEMKQFIDEKGIVCPVSGTANWTEIRQFNLMFKTFQG
VTESSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIAQVGKSFRNEITPGNFIFRTREFEQMELEFFCKPGTEIEWQE
YWKNFAAKWLKDLGIKEENTKLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLKAHMAASGDDFSYHDQET
NEKYVPYCIEPSLGADRVTLAFLCDAYEEETLEGGDSRTVMRFHPALAPYKAAILPLSKKLSPDAMKVYEALSADFAIDF
DESQSIGKRYRRQDEIGTPFCITFDFDSLEDNQVTVRDRDTMEQVRMPISEVKAFLDEKIKF

Sequences:

>Translated_462_residues
MAEKNMETIVNLAKHRGFVFPGSEIYGGLANTWDYGPLGVELKNNVKRAWWQKFVQQSPYNVGLDAAILMNPKTWEASGH
LSNFNDPMIDNKDSKIRYRADKLIEDYMHAQGDEHFIADGMSFDEMKQFIDEKGIVCPVSGTANWTEIRQFNLMFKTFQG
VTESSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIAQVGKSFRNEITPGNFIFRTREFEQMELEFFCKPGTEIEWQE
YWKNFAAKWLKDLGIKEENTKLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLKAHMAASGDDFSYHDQET
NEKYVPYCIEPSLGADRVTLAFLCDAYEEETLEGGDSRTVMRFHPALAPYKAAILPLSKKLSPDAMKVYEALSADFAIDF
DESQSIGKRYRRQDEIGTPFCITFDFDSLEDNQVTVRDRDTMEQVRMPISEVKAFLDEKIKF
>Mature_461_residues
AEKNMETIVNLAKHRGFVFPGSEIYGGLANTWDYGPLGVELKNNVKRAWWQKFVQQSPYNVGLDAAILMNPKTWEASGHL
SNFNDPMIDNKDSKIRYRADKLIEDYMHAQGDEHFIADGMSFDEMKQFIDEKGIVCPVSGTANWTEIRQFNLMFKTFQGV
TESSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIAQVGKSFRNEITPGNFIFRTREFEQMELEFFCKPGTEIEWQEY
WKNFAAKWLKDLGIKEENTKLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLKAHMAASGDDFSYHDQETN
EKYVPYCIEPSLGADRVTLAFLCDAYEEETLEGGDSRTVMRFHPALAPYKAAILPLSKKLSPDAMKVYEALSADFAIDFD
ESQSIGKRYRRQDEIGTPFCITFDFDSLEDNQVTVRDRDTMEQVRMPISEVKAFLDEKIKF

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=352, Percent_Identity=29.5454545454545, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI70887790, Length=311, Percent_Identity=25.08038585209, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32564541, Length=397, Percent_Identity=28.2115869017632, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17554996, Length=397, Percent_Identity=28.2115869017632, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6325338, Length=372, Percent_Identity=29.0322580645161, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319597, Length=391, Percent_Identity=27.1099744245524, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI21357965, Length=356, Percent_Identity=31.7415730337079, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24664462, Length=356, Percent_Identity=31.7415730337079, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_MACCJ (B9E6V6)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560620.1
- GeneID:   7389168
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_1217
- OMA:   GSEWGEL
- ProtClustDB:   PRK04173
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 53383; Mature: 53251

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 99-99 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKNMETIVNLAKHRGFVFPGSEIYGGLANTWDYGPLGVELKNNVKRAWWQKFVQQSPY
CCCCHHHHHHHHHHHCCEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
NVGLDAAILMNPKTWEASGHLSNFNDPMIDNKDSKIRYRADKLIEDYMHAQGDEHFIADG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
MSFDEMKQFIDEKGIVCPVSGTANWTEIRQFNLMFKTFQGVTESSTNEIFLRPETAQGIF
CCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEE
VNYKNVQRSMRKKLPFGIAQVGKSFRNEITPGNFIFRTREFEQMELEFFCKPGTEIEWQE
EEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCHHEEEEEEECCCCCCCHHH
YWKNFAAKWLKDLGIKEENTKLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCC
DLKAHMAASGDDFSYHDQETNEKYVPYCIEPSLGADRVTLAFLCDAYEEETLEGGDSRTV
CEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
MRFHPALAPYKAAILPLSKKLSPDAMKVYEALSADFAIDFDESQSIGKRYRRQDEIGTPF
EEECCCCCCHHHHHCCHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCE
CITFDFDSLEDNQVTVRDRDTMEQVRMPISEVKAFLDEKIKF
EEEEECCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEKNMETIVNLAKHRGFVFPGSEIYGGLANTWDYGPLGVELKNNVKRAWWQKFVQQSPY
CCCHHHHHHHHHHHCCEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
NVGLDAAILMNPKTWEASGHLSNFNDPMIDNKDSKIRYRADKLIEDYMHAQGDEHFIADG
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
MSFDEMKQFIDEKGIVCPVSGTANWTEIRQFNLMFKTFQGVTESSTNEIFLRPETAQGIF
CCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEE
VNYKNVQRSMRKKLPFGIAQVGKSFRNEITPGNFIFRTREFEQMELEFFCKPGTEIEWQE
EEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCCHHEEEEEEECCCCCCCHHH
YWKNFAAKWLKDLGIKEENTKLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCC
DLKAHMAASGDDFSYHDQETNEKYVPYCIEPSLGADRVTLAFLCDAYEEETLEGGDSRTV
CEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
MRFHPALAPYKAAILPLSKKLSPDAMKVYEALSADFAIDFDESQSIGKRYRRQDEIGTPF
EEECCCCCCHHHHHCCHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCE
CITFDFDSLEDNQVTVRDRDTMEQVRMPISEVKAFLDEKIKF
EEEEECCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA