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The map label for this gene is pyrB

Identifier: 222151019

GI number: 222151019

Start: 829255

End: 830136

Strand: Direct

Name: pyrB

Synonym: MCCL_0770

Alternate gene names: 222151019

Gene position: 829255-830136 (Clockwise)

Preceding gene: 222151018

Following gene: 222151020

Centisome position: 39.44

GC content: 40.59

Gene sequence:

>882_bases
ATGAAGCATCTGCATTCGATTACTGAACTATCTAATGATGAAATTATGCACACGATTGAACGTGCAATAATGATTAAGAA
TGGTGAAATAAAACGCTATGAAAATATTTATACGGCAAATCTCTTCTTTGAGAACTCAACGCGCACAAAATGCTCATTCG
AGATGGCAGAGAGAAAGCTCGGCCTTCAAGTCATACCGTTTGAAACGAGTACTTCAAGTGTAACGAAAGGTGAGAGTCTG
TATGATACTTGTAAGACGCTTGAGAGTATCGGTTGCGATGTACTGGTAATCAGACATCCTGAGAATAACTATTACGAGCA
GTTACACGAGCTTAACATACCGGTAGTCAACGGAGGAGATGGCAGTGGCCAGCATCCGACACAATGTCTACTGGATTTGA
TGACCATCTATGAAGAATATGGGAAGTTTGAAGGATTGAAGATAATCATCTGTGGAGATATCTTAAATTCTCGGGTAGCA
AGAAGTAATTATCATGCGCTGACGAGCCTGGGAGCACAAGTTAAATTTGTTGCACCTGAAATTTGGCAGGATCATTCATT
GGATGCTGAATATGTCAGTATTGATGATGCGATTGAAGAGGTTGATGTTTGTATGCTGCTACGTGTGCAGCATGAACGTC
ATGATGAAGATGCTGCAAACTTTTCTCGAGTCAACTATAACAGGATGTATGGGTTAACAAGAGAAAGGTATGATAGGTTG
AAAGAGGAAGCTATTATATTACACCCGGCCCCGGTGAATCGTGATGTCGAGATTGATAGTGAGCTTGTCGAAGCGCCGAA
GTCACGTATTTTTAAGCAGATGGAAAACGGTGTCTACACACGTATGAGTATTTTAAGTCAAGTCATTGAAAATAATCGAT
AG

Upstream 100 bases:

>100_bases
TAAATATCGAAGGTATGGCACTCGCAGCACTGGCTGGAATATTACTTAATGCGATATTGCCTGAATCAAAAGTACATGAA
GACTATTAAGGGGTGGAACA

Downstream 100 bases:

>100_bases
GAGTGTTGAATATGTCAACTTTATTAAGAGGCGGAAAAGTATTAGTTAATGATGAACTTGTTTCAAAGGATATTCGAATA
GAAGACGGAAAAATAGTAGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKHLHSITELSNDEIMHTIERAIMIKNGEIKRYENIYTANLFFENSTRTKCSFEMAERKLGLQVIPFETSTSSVTKGESL
YDTCKTLESIGCDVLVIRHPENNYYEQLHELNIPVVNGGDGSGQHPTQCLLDLMTIYEEYGKFEGLKIIICGDILNSRVA
RSNYHALTSLGAQVKFVAPEIWQDHSLDAEYVSIDDAIEEVDVCMLLRVQHERHDEDAANFSRVNYNRMYGLTRERYDRL
KEEAIILHPAPVNRDVEIDSELVEAPKSRIFKQMENGVYTRMSILSQVIENNR

Sequences:

>Translated_293_residues
MKHLHSITELSNDEIMHTIERAIMIKNGEIKRYENIYTANLFFENSTRTKCSFEMAERKLGLQVIPFETSTSSVTKGESL
YDTCKTLESIGCDVLVIRHPENNYYEQLHELNIPVVNGGDGSGQHPTQCLLDLMTIYEEYGKFEGLKIIICGDILNSRVA
RSNYHALTSLGAQVKFVAPEIWQDHSLDAEYVSIDDAIEEVDVCMLLRVQHERHDEDAANFSRVNYNRMYGLTRERYDRL
KEEAIILHPAPVNRDVEIDSELVEAPKSRIFKQMENGVYTRMSILSQVIENNR
>Mature_293_residues
MKHLHSITELSNDEIMHTIERAIMIKNGEIKRYENIYTANLFFENSTRTKCSFEMAERKLGLQVIPFETSTSSVTKGESL
YDTCKTLESIGCDVLVIRHPENNYYEQLHELNIPVVNGGDGSGQHPTQCLLDLMTIYEEYGKFEGLKIIICGDILNSRVA
RSNYHALTSLGAQVKFVAPEIWQDHSLDAEYVSIDDAIEEVDVCMLLRVQHERHDEDAANFSRVNYNRMYGLTRERYDRL
KEEAIILHPAPVNRDVEIDSELVEAPKSRIFKQMENGVYTRMSILSQVIENNR

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=310, Percent_Identity=34.1935483870968, Blast_Score=165, Evalue=5e-41,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=33.7620578778135, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1786469, Length=170, Percent_Identity=34.1176470588235, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790703, Length=156, Percent_Identity=33.974358974359, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=318, Percent_Identity=35.5345911949685, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=285, Percent_Identity=31.5789473684211, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24642586, Length=268, Percent_Identity=37.6865671641791, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_MACCJ (B9EB66)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560173.1
- GeneID:   7389868
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0770
- OMA:   MNRGLEI
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33711; Mature: 33711

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLHSITELSNDEIMHTIERAIMIKNGEIKRYENIYTANLFFENSTRTKCSFEMAERKL
CCHHHHHHHCCCHHHHHHHHHHHEEECCCCCHHHCEEEEEEEEECCCCCCHHHHHHHHHC
GLQVIPFETSTSSVTKGESLYDTCKTLESIGCDVLVIRHPENNYYEQLHELNIPVVNGGD
CCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEECCC
GSGQHPTQCLLDLMTIYEEYGKFEGLKIIICGDILNSRVARSNYHALTSLGAQVKFVAPE
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCHHHHHHCCCEEEEECHH
IWQDHSLDAEYVSIDDAIEEVDVCMLLRVQHERHDEDAANFSRVNYNRMYGLTRERYDRL
HCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHCCCHHHHHHH
KEEAIILHPAPVNRDVEIDSELVEAPKSRIFKQMENGVYTRMSILSQVIENNR
HHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHLHSITELSNDEIMHTIERAIMIKNGEIKRYENIYTANLFFENSTRTKCSFEMAERKL
CCHHHHHHHCCCHHHHHHHHHHHEEECCCCCHHHCEEEEEEEEECCCCCCHHHHHHHHHC
GLQVIPFETSTSSVTKGESLYDTCKTLESIGCDVLVIRHPENNYYEQLHELNIPVVNGGD
CCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEECCC
GSGQHPTQCLLDLMTIYEEYGKFEGLKIIICGDILNSRVARSNYHALTSLGAQVKFVAPE
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCHHHHHHCCCEEEEECHH
IWQDHSLDAEYVSIDDAIEEVDVCMLLRVQHERHDEDAANFSRVNYNRMYGLTRERYDRL
HCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHCCCHHHHHHH
KEEAIILHPAPVNRDVEIDSELVEAPKSRIFKQMENGVYTRMSILSQVIENNR
HHCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA