Click here to switch to the map view.

The map label for this gene is pyrP [H]

Identifier: 222151018

GI number: 222151018

Start: 827933

End: 829243

Strand: Direct

Name: pyrP [H]

Synonym: MCCL_0769

Alternate gene names: 222151018

Gene position: 827933-829243 (Clockwise)

Preceding gene: 222151017

Following gene: 222151019

Centisome position: 39.38

GC content: 41.27

Gene sequence:

>1311_bases
ATGACAAACGAAGAAATTTATGAACGAACAGTTGAACCAATTTTAGATGTACATGAGAAACCGAAAGCAAGTGAGTGGTT
ACTCTTAAGTTCTCAGCACTTATTTGCAATGTTTGGTGCAACTGTACTTGTACCTTTTCTGACGGGGCTACCTGTATCAG
CAGCGCTTATCGCTTCAGGGTTAGGAACTTTGCTCTATATATTGATTACTAAAGGAAAGATTCCTGCATATCTTGGCTCA
AGCTTTGCATTTATCACACCGATTATAGTGGGATTAAAAACACATAGTCTAGGTGAAATGTTGATGGCGCTTTTTATGAG
TGGTCTGATGTATGTGATTATCGGAATAGTCATCAAGTTTGTAGGTGTGAACTGGCTGCTTAGATTACTGCCACCTGTTG
TTGTCGGACCTGTTATTATGGTCATCGGACTTGGACTAGCACCTGTAGCTGTCAACATGGCAATGTATACAAATAGTGGG
ACAATGGAAGGATATAGCATTAAATATTTATTTATTGCATTCGTAACACTCGTTACAGTGATTATCTTCTCGGTGCTCGT
TCGAGGCTTCCTGTCAATCATTCCAGTACTCATTGGCATAATTGTCGGATATATCACAGCAATAGTTCTTGGTGTCGTAG
ATCTAAGCGGCATTCAAAAAGCAAAATGGTTCCAGCTTCCAGATGTACATGTTCCGTTCGTAAGTTATACACCGAGCATT
GACTGGATGTTTGTCCTTATTATGCTGCCGATCGTCTTCGTAACAGTGAGTGAACACATCGGACACCAAGTTGTGATTAA
TAAGATTGTCGGACGTAACTTCTTTAAAGATCCGGGATTACATCGCAGCTTAATTGGAGACGGTGTTTCAACGATGCTCG
CTTCAATCATCGGTGGACCCCCGAGCACGACATATGGTGAGAACATCGGGGTGCTTGCGATTACACGTATCTATAGTATA
TGGGTTATCGGAGGAGCAGCGGTACTTGCTGTGATTCTTGGATTTGTAGGTAAGTTTACAGCTCTCGTATCAAGTATTCC
GACACCGGTTATGGGTGGTGTCTCAATATTATTATTCGGGATTATCGCTTCAAGTGGCTTAAGGATGCTCGTCGAATCCA
AAGTGGATTTTGGGGATAAACGCAATTTAGTGATTGCTTCAGTCATATTAGTGCTGGGTATCGGTAAAGCACATCTTGAC
TTCAGTGTCGGTCAAATTAACTTAAATATCGAAGGTATGGCACTCGCAGCACTGGCTGGAATATTACTTAATGCGATATT
GCCTGAATCAAAAGTACATGAAGACTATTAA

Upstream 100 bases:

>100_bases
TAAATTGGTACGAGAGACCAGCAAAGGGTTTAAGTGATAACAGACACTTAAGTCTCTTTGCGTACGCAAAGAGACTTTTT
TTATTATATGGAGGGACATT

Downstream 100 bases:

>100_bases
GGGGTGGAACAATGAAGCATCTGCATTCGATTACTGAACTATCTAATGATGAAATTATGCACACGATTGAACGTGCAATA
ATGATTAAGAATGGTGAAAT

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTNEEIYERTVEPILDVHEKPKASEWLLLSSQHLFAMFGATVLVPFLTGLPVSAALIASGLGTLLYILITKGKIPAYLGS
SFAFITPIIVGLKTHSLGEMLMALFMSGLMYVIIGIVIKFVGVNWLLRLLPPVVVGPVIMVIGLGLAPVAVNMAMYTNSG
TMEGYSIKYLFIAFVTLVTVIIFSVLVRGFLSIIPVLIGIIVGYITAIVLGVVDLSGIQKAKWFQLPDVHVPFVSYTPSI
DWMFVLIMLPIVFVTVSEHIGHQVVINKIVGRNFFKDPGLHRSLIGDGVSTMLASIIGGPPSTTYGENIGVLAITRIYSI
WVIGGAAVLAVILGFVGKFTALVSSIPTPVMGGVSILLFGIIASSGLRMLVESKVDFGDKRNLVIASVILVLGIGKAHLD
FSVGQINLNIEGMALAALAGILLNAILPESKVHEDY

Sequences:

>Translated_436_residues
MTNEEIYERTVEPILDVHEKPKASEWLLLSSQHLFAMFGATVLVPFLTGLPVSAALIASGLGTLLYILITKGKIPAYLGS
SFAFITPIIVGLKTHSLGEMLMALFMSGLMYVIIGIVIKFVGVNWLLRLLPPVVVGPVIMVIGLGLAPVAVNMAMYTNSG
TMEGYSIKYLFIAFVTLVTVIIFSVLVRGFLSIIPVLIGIIVGYITAIVLGVVDLSGIQKAKWFQLPDVHVPFVSYTPSI
DWMFVLIMLPIVFVTVSEHIGHQVVINKIVGRNFFKDPGLHRSLIGDGVSTMLASIIGGPPSTTYGENIGVLAITRIYSI
WVIGGAAVLAVILGFVGKFTALVSSIPTPVMGGVSILLFGIIASSGLRMLVESKVDFGDKRNLVIASVILVLGIGKAHLD
FSVGQINLNIEGMALAALAGILLNAILPESKVHEDY
>Mature_435_residues
TNEEIYERTVEPILDVHEKPKASEWLLLSSQHLFAMFGATVLVPFLTGLPVSAALIASGLGTLLYILITKGKIPAYLGSS
FAFITPIIVGLKTHSLGEMLMALFMSGLMYVIIGIVIKFVGVNWLLRLLPPVVVGPVIMVIGLGLAPVAVNMAMYTNSGT
MEGYSIKYLFIAFVTLVTVIIFSVLVRGFLSIIPVLIGIIVGYITAIVLGVVDLSGIQKAKWFQLPDVHVPFVSYTPSID
WMFVLIMLPIVFVTVSEHIGHQVVINKIVGRNFFKDPGLHRSLIGDGVSTMLASIIGGPPSTTYGENIGVLAITRIYSIW
VIGGAAVLAVILGFVGKFTALVSSIPTPVMGGVSILLFGIIASSGLRMLVESKVDFGDKRNLVIASVILVLGIGKAHLDF
SVGQINLNIEGMALAALAGILLNAILPESKVHEDY

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=463, Percent_Identity=24.622030237581, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI40316845, Length=463, Percent_Identity=24.622030237581, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI44680145, Length=479, Percent_Identity=24.6346555323591, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI44680143, Length=480, Percent_Identity=24.375, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788843, Length=414, Percent_Identity=47.1014492753623, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI87081818, Length=439, Percent_Identity=36.6742596810934, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI87082181, Length=452, Percent_Identity=28.0973451327434, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790087, Length=459, Percent_Identity=27.2331154684096, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87082178, Length=465, Percent_Identity=27.5268817204301, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17541904, Length=440, Percent_Identity=24.5454545454545, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17542262, Length=385, Percent_Identity=24.4155844155844, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71993493, Length=390, Percent_Identity=22.3076923076923, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17542260, Length=237, Percent_Identity=24.4725738396624, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558856, Length=176, Percent_Identity=26.7045454545455, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21356175, Length=444, Percent_Identity=26.5765765765766, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 46656; Mature: 46525

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TNEEIYERTVEPILDVHEKPKASEWLLLSSQHLFAMFGATVLVPFLTGLPVSAALIASG
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LGTLLYILITKGKIPAYLGSSFAFITPIIVGLKTHSLGEMLMALFMSGLMYVIIGIVIKF
HHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVNWLLRLLPPVVVGPVIMVIGLGLAPVAVNMAMYTNSGTMEGYSIKYLFIAFVTLVTV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHH
IIFSVLVRGFLSIIPVLIGIIVGYITAIVLGVVDLSGIQKAKWFQLPDVHVPFVSYTPSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEECCCCH
DWMFVLIMLPIVFVTVSEHIGHQVVINKIVGRNFFKDPGLHRSLIGDGVSTMLASIIGGP
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHCCC
PSTTYGENIGVLAITRIYSIWVIGGAAVLAVILGFVGKFTALVSSIPTPVMGGVSILLFG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IIASSGLRMLVESKVDFGDKRNLVIASVILVLGIGKAHLDFSVGQINLNIEGMALAALAG
HHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCEEEEEEEEEECHHHHHHHHH
ILLNAILPESKVHEDY
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8206848 [H]