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The map label for this gene is purH

Identifier: 222150946

GI number: 222150946

Start: 748340

End: 749818

Strand: Direct

Name: purH

Synonym: MCCL_0696

Alternate gene names: 222150946

Gene position: 748340-749818 (Clockwise)

Preceding gene: 222150945

Following gene: 222150947

Centisome position: 35.6

GC content: 44.62

Gene sequence:

>1479_bases
ATGAGAAAAGCATTATTAAGTGTATCAGATAAAACAGGTATTATCCCATTTGCCGAACGTTTAACAGAACTTGACTTTGA
ACTTTATTCAACAGGTGGTACGAAAAAGGCATTAGAAGACAATGATATTGCGGTAAAGTCAGTCAGTGAACTAACAAACT
TTCCTGAAATCATGGACGGACGTGTGAAGACGTTACACCCGAACATTCACGGTGGGATATTAGCGGACCGCAACAATCCT
GAGCACATAAAGGCAATGGAGACACACGGTATTCAGCCGTTAGATCTAGTGGTCGTGAATCTCTATCCGTTTGAAGCGAC
AGTTGCCAACCCTGATGTTACTGAAATGGATGCCATCGAGAATATCGATATCGGTGGACCGACAATGCTACGTTCATCAG
CAAAGAACTTCAGGCATGTCATCACTGTTGTAGACCCGGCAGATTATGATGAAGTCATCGAAAAATTAAAAACCGATACT
CTAGACGAGACATTCCGTAAACAGCTGATGATCAAAGTGTTCACGCATACGAACAAATATGACGCTGCAATCGTCAATTT
CTTCAGCGATAATACATCAACGCTGCGTTACGGAGAAAACCCGCATCAGAAGGCGCGTTTCATCAAGACAGACGCAAAAC
CGAACACGTTAGCTGGTGCACGCGTTCTGCACGGCAAACCGCTGAGCTTTAACAATATTAAAGATGCAGATGCAACGCTG
TTCTTAGTGAAGCAGTTCGATATGCCTGCTGCAGTTGCAGTGAAACATATGAACCCTTGTGGTGTCGGAACAGGTGAAGT
CATCAGCGAAGCTTTCCAGAATGCCTATGATGCAGATAGCCAGAGTATCTTCGGAGGTATCGTCGCATTAAACCGTGAAG
TGGATAAAGCAACTGCTGAAAAGATGCATGCCATCTTCCTTGAAGTGATCATCGCGCCGAAGTTCAGCGACGAGGCACTT
GAAATCTTAACGGCGAAGAAGAATATTCGTCTTCTTGAAATCGATATGGATATGGACAAAGATGAAGAAGAGTTTGTATC
TGTCAGTGGTGGTTATCTCGTTCAGGATAAAGATCTGCTGAACGTAACGCGCGAAGACATGCGTGTCGTGACGAACACTG
AACCGACAGAAGCACAATGGAAAGCAATTGAGCTCGGATGGAAAGTCGTGAAAAGCGTGAAAAGTAATGCCATCGTCCTT
GCAAATGATAAGCAGACAGTAGGAATTGGTGCAGGTCAGATGAACCGTGTCGGTGCAGCGAAGATTGCCATCGAACGTGC
AATTGAAATGAACGACAATGTTGTCCTTGCAAGTGACGGCTTCTTCCCAATGTCAGATACGGTCGAAACGGCGTATGCTG
CAGGTATTAAATGTATCGTTCAGCCAGGGGGCTCTATCAAGGATCAGGATTCGATCGATAAAGCGAATGAATACGGCATC
GCAATGGTAATGACGGATGTCAGACACTTTAAACATTAG

Upstream 100 bases:

>100_bases
AAGAAGATACAGAGGAGACATTGCAGCTCCGAATTCAGAATCTAGAATACGAACTCTACCCGCGCGTCATCAAGAAAATT
ATTCGATAAGGAGTCTACTT

Downstream 100 bases:

>100_bases
GAGGCAGCTATGAATATATTAGTAATCGGTAGTGGGGGTCGTGAGCATGCACTGGCAAGAAAGCTCGCACAGTCACCACG
TGTCACGGAAGTGTTCGTTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MRKALLSVSDKTGIIPFAERLTELDFELYSTGGTKKALEDNDIAVKSVSELTNFPEIMDGRVKTLHPNIHGGILADRNNP
EHIKAMETHGIQPLDLVVVNLYPFEATVANPDVTEMDAIENIDIGGPTMLRSSAKNFRHVITVVDPADYDEVIEKLKTDT
LDETFRKQLMIKVFTHTNKYDAAIVNFFSDNTSTLRYGENPHQKARFIKTDAKPNTLAGARVLHGKPLSFNNIKDADATL
FLVKQFDMPAAVAVKHMNPCGVGTGEVISEAFQNAYDADSQSIFGGIVALNREVDKATAEKMHAIFLEVIIAPKFSDEAL
EILTAKKNIRLLEIDMDMDKDEEEFVSVSGGYLVQDKDLLNVTREDMRVVTNTEPTEAQWKAIELGWKVVKSVKSNAIVL
ANDKQTVGIGAGQMNRVGAAKIAIERAIEMNDNVVLASDGFFPMSDTVETAYAAGIKCIVQPGGSIKDQDSIDKANEYGI
AMVMTDVRHFKH

Sequences:

>Translated_492_residues
MRKALLSVSDKTGIIPFAERLTELDFELYSTGGTKKALEDNDIAVKSVSELTNFPEIMDGRVKTLHPNIHGGILADRNNP
EHIKAMETHGIQPLDLVVVNLYPFEATVANPDVTEMDAIENIDIGGPTMLRSSAKNFRHVITVVDPADYDEVIEKLKTDT
LDETFRKQLMIKVFTHTNKYDAAIVNFFSDNTSTLRYGENPHQKARFIKTDAKPNTLAGARVLHGKPLSFNNIKDADATL
FLVKQFDMPAAVAVKHMNPCGVGTGEVISEAFQNAYDADSQSIFGGIVALNREVDKATAEKMHAIFLEVIIAPKFSDEAL
EILTAKKNIRLLEIDMDMDKDEEEFVSVSGGYLVQDKDLLNVTREDMRVVTNTEPTEAQWKAIELGWKVVKSVKSNAIVL
ANDKQTVGIGAGQMNRVGAAKIAIERAIEMNDNVVLASDGFFPMSDTVETAYAAGIKCIVQPGGSIKDQDSIDKANEYGI
AMVMTDVRHFKH
>Mature_492_residues
MRKALLSVSDKTGIIPFAERLTELDFELYSTGGTKKALEDNDIAVKSVSELTNFPEIMDGRVKTLHPNIHGGILADRNNP
EHIKAMETHGIQPLDLVVVNLYPFEATVANPDVTEMDAIENIDIGGPTMLRSSAKNFRHVITVVDPADYDEVIEKLKTDT
LDETFRKQLMIKVFTHTNKYDAAIVNFFSDNTSTLRYGENPHQKARFIKTDAKPNTLAGARVLHGKPLSFNNIKDADATL
FLVKQFDMPAAVAVKHMNPCGVGTGEVISEAFQNAYDADSQSIFGGIVALNREVDKATAEKMHAIFLEVIIAPKFSDEAL
EILTAKKNIRLLEIDMDMDKDEEEFVSVSGGYLVQDKDLLNVTREDMRVVTNTEPTEAQWKAIELGWKVVKSVKSNAIVL
ANDKQTVGIGAGQMNRVGAAKIAIERAIEMNDNVVLASDGFFPMSDTVETAYAAGIKCIVQPGGSIKDQDSIDKANEYGI
AMVMTDVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=465, Percent_Identity=39.3548387096774, Blast_Score=271, Evalue=9e-73,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=48, Blast_Score=458, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71985564, Length=466, Percent_Identity=36.2660944206009, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI71985574, Length=298, Percent_Identity=29.5302013422819, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323768, Length=493, Percent_Identity=34.8884381338742, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323056, Length=459, Percent_Identity=35.5119825708061, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI24649832, Length=469, Percent_Identity=37.1002132196162, Blast_Score=258, Evalue=7e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_MACCJ (B9EAZ2)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560099.1
- GeneID:   7389855
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0696
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 54248; Mature: 54248

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKALLSVSDKTGIIPFAERLTELDFELYSTGGTKKALEDNDIAVKSVSELTNFPEIMDG
CCCCEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHCC
RVKTLHPNIHGGILADRNNPEHIKAMETHGIQPLDLVVVNLYPFEATVANPDVTEMDAIE
CEEEECCCCCCCEEECCCCCHHEEHHHHCCCCEEEEEEEEEECCEEEECCCCCHHHHHHC
NIDIGGPTMLRSSAKNFRHVITVVDPADYDEVIEKLKTDTLDETFRKQLMIKVFTHTNKY
CCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHEEHEEECCCCC
DAAIVNFFSDNTSTLRYGENPHQKARFIKTDAKPNTLAGARVLHGKPLSFNNIKDADATL
CEEEEEEECCCCCEEEECCCHHHHHHEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEE
FLVKQFDMPAAVAVKHMNPCGVGTGEVISEAFQNAYDADSQSIFGGIVALNREVDKATAE
EEEEECCCCHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHEEECCHHHHHHHH
KMHAIFLEVIIAPKFSDEALEILTAKKNIRLLEIDMDMDKDEEEFVSVSGGYLVQDKDLL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHEEECCCEEEECCHHH
NVTREDMRVVTNTEPTEAQWKAIELGWKVVKSVKSNAIVLANDKQTVGIGAGQMNRVGAA
HHCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCHH
KIAIERAIEMNDNVVLASDGFFPMSDTVETAYAAGIKCIVQPGGSIKDQDSIDKANEYGI
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHCCCE
AMVMTDVRHFKH
EEEEEHHHHHCC
>Mature Secondary Structure
MRKALLSVSDKTGIIPFAERLTELDFELYSTGGTKKALEDNDIAVKSVSELTNFPEIMDG
CCCCEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHCC
RVKTLHPNIHGGILADRNNPEHIKAMETHGIQPLDLVVVNLYPFEATVANPDVTEMDAIE
CEEEECCCCCCCEEECCCCCHHEEHHHHCCCCEEEEEEEEEECCEEEECCCCCHHHHHHC
NIDIGGPTMLRSSAKNFRHVITVVDPADYDEVIEKLKTDTLDETFRKQLMIKVFTHTNKY
CCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHEEHEEECCCCC
DAAIVNFFSDNTSTLRYGENPHQKARFIKTDAKPNTLAGARVLHGKPLSFNNIKDADATL
CEEEEEEECCCCCEEEECCCHHHHHHEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEE
FLVKQFDMPAAVAVKHMNPCGVGTGEVISEAFQNAYDADSQSIFGGIVALNREVDKATAE
EEEEECCCCHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHEEECCHHHHHHHH
KMHAIFLEVIIAPKFSDEALEILTAKKNIRLLEIDMDMDKDEEEFVSVSGGYLVQDKDLL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHEEECCCEEEECCHHH
NVTREDMRVVTNTEPTEAQWKAIELGWKVVKSVKSNAIVLANDKQTVGIGAGQMNRVGAA
HHCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCHH
KIAIERAIEMNDNVVLASDGFFPMSDTVETAYAAGIKCIVQPGGSIKDQDSIDKANEYGI
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHCCCE
AMVMTDVRHFKH
EEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA