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The map label for this gene is purN [H]

Identifier: 222150945

GI number: 222150945

Start: 747762

End: 748328

Strand: Direct

Name: purN [H]

Synonym: MCCL_0695

Alternate gene names: 222150945

Gene position: 747762-748328 (Clockwise)

Preceding gene: 222150944

Following gene: 222150946

Centisome position: 35.57

GC content: 44.62

Gene sequence:

>567_bases
ATGATTAAAGTCGCGATATTTGCTTCAGGTAATGGGTCGAATTACGAGAAGATTATGGAGCATATTCAGGCAGGATTCCT
GGATCATATTGAAGTGACAGGCCTCTATACAGATAAACGTAGCGCGTTCGCAATTGAGCGCGCACGTCGTTTTGATACGC
CTGTACACGTATTTGAACTAAAAACGTTTAACGATAAAACAGCATATGAAACAGCGATACTGAAACAATTAAAGCAGGAC
GGTGTGGAATGGGTCATTCTTGCCGGCTATATGAAGCTTGTTGGCAGGACGCTGCTCGATGCGTATGAAGGAAAGATGAT
CAACATCCATCCGTCGATATTACCAAGCTTTCCTGGTGTTAATGCTGTTGGACAGGCGCTGGATTATGGCTGCCGTGTCA
GTGGTGCTACTGTCCATTACGTTGATAGCGGCATGGATACAGGTAAGATCATCGACCAGATGAGCTGTCCGATCTACGAA
GAAGATACAGAGGAGACATTGCAGCTCCGAATTCAGAATCTAGAATACGAACTCTACCCGCGCGTCATCAAGAAAATTAT
TCGATAA

Upstream 100 bases:

>100_bases
GAAGCGGTAGAGAAGGTACGCTCAGCAGTGAACGAAGAAGTATTTGAAATCGGTCGTGTCGTACGCGGTGACAAAGCAAT
CGACATTAAAGGTGTGTAAT

Downstream 100 bases:

>100_bases
GGAGTCTACTTATGAGAAAAGCATTATTAAGTGTATCAGATAAAACAGGTATTATCCCATTTGCCGAACGTTTAACAGAA
CTTGACTTTGAACTTTATTC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MIKVAIFASGNGSNYEKIMEHIQAGFLDHIEVTGLYTDKRSAFAIERARRFDTPVHVFELKTFNDKTAYETAILKQLKQD
GVEWVILAGYMKLVGRTLLDAYEGKMINIHPSILPSFPGVNAVGQALDYGCRVSGATVHYVDSGMDTGKIIDQMSCPIYE
EDTEETLQLRIQNLEYELYPRVIKKIIR

Sequences:

>Translated_188_residues
MIKVAIFASGNGSNYEKIMEHIQAGFLDHIEVTGLYTDKRSAFAIERARRFDTPVHVFELKTFNDKTAYETAILKQLKQD
GVEWVILAGYMKLVGRTLLDAYEGKMINIHPSILPSFPGVNAVGQALDYGCRVSGATVHYVDSGMDTGKIIDQMSCPIYE
EDTEETLQLRIQNLEYELYPRVIKKIIR
>Mature_188_residues
MIKVAIFASGNGSNYEKIMEHIQAGFLDHIEVTGLYTDKRSAFAIERARRFDTPVHVFELKTFNDKTAYETAILKQLKQD
GVEWVILAGYMKLVGRTLLDAYEGKMINIHPSILPSFPGVNAVGQALDYGCRVSGATVHYVDSGMDTGKIIDQMSCPIYE
EDTEETLQLRIQNLEYELYPRVIKKIIR

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=32.0652173913043, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=32.0652173913043, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=32.0652173913043, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1788846, Length=183, Percent_Identity=31.1475409836066, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787483, Length=143, Percent_Identity=31.4685314685315, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=182, Percent_Identity=32.967032967033, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21282; Mature: 21282

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVAIFASGNGSNYEKIMEHIQAGFLDHIEVTGLYTDKRSAFAIERARRFDTPVHVFEL
CEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEEEE
KTFNDKTAYETAILKQLKQDGVEWVILAGYMKLVGRTLLDAYEGKMINIHPSILPSFPGV
EECCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCH
NAVGQALDYGCRVSGATVHYVDSGMDTGKIIDQMSCPIYEEDTEETLQLRIQNLEYELYP
HHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
RVIKKIIR
HHHHHHHC
>Mature Secondary Structure
MIKVAIFASGNGSNYEKIMEHIQAGFLDHIEVTGLYTDKRSAFAIERARRFDTPVHVFEL
CEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEEEE
KTFNDKTAYETAILKQLKQDGVEWVILAGYMKLVGRTLLDAYEGKMINIHPSILPSFPGV
EECCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCH
NAVGQALDYGCRVSGATVHYVDSGMDTGKIIDQMSCPIYEEDTEETLQLRIQNLEYELYP
HHHHHHHHCCCEECCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
RVIKKIIR
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA