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The map label for this gene is purD [H]

Identifier: 222150947

GI number: 222150947

Start: 749828

End: 751063

Strand: Direct

Name: purD [H]

Synonym: MCCL_0697

Alternate gene names: 222150947

Gene position: 749828-751063 (Clockwise)

Preceding gene: 222150946

Following gene: 222150948

Centisome position: 35.67

GC content: 44.01

Gene sequence:

>1236_bases
ATGAATATATTAGTAATCGGTAGTGGGGGTCGTGAGCATGCACTGGCAAGAAAGCTCGCACAGTCACCACGTGTCACGGA
AGTGTTCGTTATTAAAGGGAATGATGCGATGGCCCGCGAAGCAACGATAGTAGATATCGATGAAGATGATCATGATGCTA
TCGTACAGTTTGCGCAAGATAACGCCATCGAACTTGTCGTTGTCGGCCCTGAACAGCCATTGATCGATGGCTTAAGCGAT
CGATTAGATGAAGCAGGCATTAAAGTGTTTGGTCCGAACAAGAAGGCTGCCCAGATGGAAGGTTCGAAAGACTTTGCAAA
AGCATTGATGAAAAGATATGACATCCCAACTGCAAGCTATGAGACGATTGATAACAAAACAGATGCACTTGAATATCTAG
ATGAAAAAGGGACTCCGATTGTCATCAAATACGACGGTCTTGCTGCCGGTAAAGGTGTGGTTGTTGCGATGGATCGTGAT
ATGGCAGTTGATGCTATCAATGATTTCTATGCTGAAGCAGGTGCGAAAGTCGTCTTTGAAGAATATCTTGAAGGGGAAGA
ATATTCACTCATGGTGCTGGTGAATGATGATTTCTGTATCCCGTTTGATACGATCGCACAAGACCATAAACGTGCTTTTG
ATCATGATGAAGGTCCGAACACTGGCGGTATGGGAGCATACTGTCCAGTAAATCATATCTCTGATGATGTCCTTAAGACG
ACATATGATACAATCGTCTATCCGACAGTGCGTGCAATGCACGAAGAAGGGCTGAACTATTTCGGTGTCCTATATGTCGG
TGCGATTCTTACAGCTGATGGACCAAAAGTCATTGAATTTAACGCACGTTTCGGTGATCCAGAAGCACAAGTGCTATTAA
GACGTCTGGAAACAGATCTCGTAGCGCTGATTGAAGCAGCATATCATAAAACAGAATTAGACTTAGTATGGCGTAACAAA
TCAATATGTGGTGTCGTACTGGCATCGAATGGTTACCCAGGGAGTTACGCTAAAGGTGCAGTCGTAGAAGGCTATGATTA
TCAGGATGACTATATTGTGAGTGCATTAAAGCGCGTTGATGGCACGTGGGTAACGAGCGGAGGACGTGTGATGCTGGCAT
TCGGTGAAGGTGATAATCTTCAGCAGGCACAGCAGGCAGCATATGAAAATGTCAAGAAAATTAAGAGTGAGGGTCTATTC
TACAGAACAGATATCGGAAATAAAGGTCTGAAATAA

Upstream 100 bases:

>100_bases
GGGCTCTATCAAGGATCAGGATTCGATCGATAAAGCGAATGAATACGGCATCGCAATGGTAATGACGGATGTCAGACACT
TTAAACATTAGGAGGCAGCT

Downstream 100 bases:

>100_bases
AATACACAATAGAGGCTGGGACATTTGTCCCAGCCTCTATTGTATTAAACGGCAGAACACATTTGTCCCAGCCTCTATTT
ATTCAAATGTTTTTTGAACG

Product: phosphoribosylamine-glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNILVIGSGGREHALARKLAQSPRVTEVFVIKGNDAMAREATIVDIDEDDHDAIVQFAQDNAIELVVVGPEQPLIDGLSD
RLDEAGIKVFGPNKKAAQMEGSKDFAKALMKRYDIPTASYETIDNKTDALEYLDEKGTPIVIKYDGLAAGKGVVVAMDRD
MAVDAINDFYAEAGAKVVFEEYLEGEEYSLMVLVNDDFCIPFDTIAQDHKRAFDHDEGPNTGGMGAYCPVNHISDDVLKT
TYDTIVYPTVRAMHEEGLNYFGVLYVGAILTADGPKVIEFNARFGDPEAQVLLRRLETDLVALIEAAYHKTELDLVWRNK
SICGVVLASNGYPGSYAKGAVVEGYDYQDDYIVSALKRVDGTWVTSGGRVMLAFGEGDNLQQAQQAAYENVKKIKSEGLF
YRTDIGNKGLK

Sequences:

>Translated_411_residues
MNILVIGSGGREHALARKLAQSPRVTEVFVIKGNDAMAREATIVDIDEDDHDAIVQFAQDNAIELVVVGPEQPLIDGLSD
RLDEAGIKVFGPNKKAAQMEGSKDFAKALMKRYDIPTASYETIDNKTDALEYLDEKGTPIVIKYDGLAAGKGVVVAMDRD
MAVDAINDFYAEAGAKVVFEEYLEGEEYSLMVLVNDDFCIPFDTIAQDHKRAFDHDEGPNTGGMGAYCPVNHISDDVLKT
TYDTIVYPTVRAMHEEGLNYFGVLYVGAILTADGPKVIEFNARFGDPEAQVLLRRLETDLVALIEAAYHKTELDLVWRNK
SICGVVLASNGYPGSYAKGAVVEGYDYQDDYIVSALKRVDGTWVTSGGRVMLAFGEGDNLQQAQQAAYENVKKIKSEGLF
YRTDIGNKGLK
>Mature_411_residues
MNILVIGSGGREHALARKLAQSPRVTEVFVIKGNDAMAREATIVDIDEDDHDAIVQFAQDNAIELVVVGPEQPLIDGLSD
RLDEAGIKVFGPNKKAAQMEGSKDFAKALMKRYDIPTASYETIDNKTDALEYLDEKGTPIVIKYDGLAAGKGVVVAMDRD
MAVDAINDFYAEAGAKVVFEEYLEGEEYSLMVLVNDDFCIPFDTIAQDHKRAFDHDEGPNTGGMGAYCPVNHISDDVLKT
TYDTIVYPTVRAMHEEGLNYFGVLYVGAILTADGPKVIEFNARFGDPEAQVLLRRLETDLVALIEAAYHKTELDLVWRNK
SICGVVLASNGYPGSYAKGAVVEGYDYQDDYIVSALKRVDGTWVTSGGRVMLAFGEGDNLQQAQQAAYENVKKIKSEGLF
YRTDIGNKGLK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=42.7230046948357, Blast_Score=325, Evalue=3e-89,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=42.7230046948357, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=42.7230046948357, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=42.7230046948357, Blast_Score=325, Evalue=5e-89,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=45.5399061032864, Blast_Score=347, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=38.8625592417062, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=40.4494382022472, Blast_Score=283, Evalue=5e-77,
Organism=Drosophila melanogaster, GI62471619, Length=435, Percent_Identity=41.8390804597701, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24582400, Length=435, Percent_Identity=41.8390804597701, Blast_Score=290, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45081; Mature: 45081

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVIGSGGREHALARKLAQSPRVTEVFVIKGNDAMAREATIVDIDEDDHDAIVQFAQD
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHEEECC
NAIELVVVGPEQPLIDGLSDRLDEAGIKVFGPNKKAAQMEGSKDFAKALMKRYDIPTASY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHCCCCHHHHHHHHHHCCCCCCCH
ETIDNKTDALEYLDEKGTPIVIKYDGLAAGKGVVVAMDRDMAVDAINDFYAEAGAKVVFE
HHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHH
EYLEGEEYSLMVLVNDDFCIPFDTIAQDHKRAFDHDEGPNTGGMGAYCPVNHISDDVLKT
HHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TYDTIVYPTVRAMHEEGLNYFGVLYVGAILTADGPKVIEFNARFGDPEAQVLLRRLETDL
HHHHHHHHHHHHHHHCCCCEEEHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
VALIEAAYHKTELDLVWRNKSICGVVLASNGYPGSYAKGAVVEGYDYQDDYIVSALKRVD
HHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHCC
GTWVTSGGRVMLAFGEGDNLQQAQQAAYENVKKIKSEGLFYRTDIGNKGLK
CCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MNILVIGSGGREHALARKLAQSPRVTEVFVIKGNDAMAREATIVDIDEDDHDAIVQFAQD
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHEEECC
NAIELVVVGPEQPLIDGLSDRLDEAGIKVFGPNKKAAQMEGSKDFAKALMKRYDIPTASY
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHCCCCHHHHHHHHHHCCCCCCCH
ETIDNKTDALEYLDEKGTPIVIKYDGLAAGKGVVVAMDRDMAVDAINDFYAEAGAKVVFE
HHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHH
EYLEGEEYSLMVLVNDDFCIPFDTIAQDHKRAFDHDEGPNTGGMGAYCPVNHISDDVLKT
HHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TYDTIVYPTVRAMHEEGLNYFGVLYVGAILTADGPKVIEFNARFGDPEAQVLLRRLETDL
HHHHHHHHHHHHHHHCCCCEEEHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
VALIEAAYHKTELDLVWRNKSICGVVLASNGYPGSYAKGAVVEGYDYQDDYIVSALKRVD
HHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHCC
GTWVTSGGRVMLAFGEGDNLQQAQQAAYENVKKIKSEGLFYRTDIGNKGLK
CCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA