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The map label for this gene is pgk

Identifier: 222150772

GI number: 222150772

Start: 554478

End: 555668

Strand: Direct

Name: pgk

Synonym: MCCL_0522

Alternate gene names: 222150772

Gene position: 554478-555668 (Clockwise)

Preceding gene: 222150771

Following gene: 222150773

Centisome position: 26.37

GC content: 39.13

Gene sequence:

>1191_bases
ATGGTTAAAAAGGTTGTTTCAGATTTAGAGTTAAAAGATAAAAAAGTACTCGTGCGTGCAGATTTCAATGTACCGATGAA
AGATGGGAACATTACGAACGATAATCGTATCGTTGAAGCATTACCTACTATTAAATATATTATCGAGCAAGGCGGGAAAG
TGATATTATTCTCTCACCTTGGTAAAGTGAAAACTGAAGAAGATAAAGCGAATTTATCACTTCAACCTGTAGCAAACCGT
TTATCAGAATTACTGGATAAAGAGGTTCAGTTTGTAGCTGAAACACGCGGAGAAAAGTTAGAGTCAGCAATTAACGCATT
ACAACCAGGTGACGTATTATTATTCGAAAACACACGTTTCGAAGATGTTGATGGTAAGAAAGAAAGTAAGAATGATGCTG
AACTTGGGAAGTACTGGGCATCACTTGGCGATGTATTTGTTAATGACGCTTTCGGTACAGCTCACCGTGAGCATGCATCG
AATGTCGGTGTTGCGACGCATCTAGATACTGCTGCAGGATTCTTGATGGAAAAAGAAATTAAGTTTATCGGTGGGGTCGT
TGAAAATCCAGAACGTCCTTTCGTTGCAATTTTAGGTGGTGCGAAAGTATCAGATAAAATCGGTGTTATCGAGAACTTAT
TAGAAGTGGCTGATAAAGTATTGATCGGTGGCGGCATGGCGTACACATTCTTGAAAGCACAAGGTAAAGAAATCGGTCAT
TCATTATTAGAGGAAGATAAAATTGACTTAGCGAAAGATTTAATGGAACGCGGTAAAGATAAACTTGTTCTACCTATTGA
TGCGAAAGTGACAAAAGAGTTCTCAAATGATGGAGAAATCGAAGCAGTAGCAATCGATCACATTCCAGCTGACTTACAAT
CTTTAGATATCGGTCCTAAAACAGTCGAATTATTCGGCAAAGAACTTGAAGGTGCTAAAACTGTTGTATGGAACGGACCG
ATGGGTGTGTTCGAAATGAGTAACTTTGCGAAAGGTACTGTAGGAGTATGTAAAGCAATTGCTGAACTGAAAGGTGCTAC
TACAATTATTGGTGGTGGAGATTCAGCAGCAGCAGCAATGGACCTTGGATTCGCTGATAAGTTTACACATATCTCAACAG
GCGGTGGCGCTTCGTTAGAGTACTTAGAAGGTAAAGAACTACCAGGTATTAAATCAATTTCTGACAAATAA

Upstream 100 bases:

>100_bases
ATTCACGCGTAAGCGGGGGAAGATTTCGCTTCTCCTGCTTTTTTTAATAAGTTATTTGGTATAATTATATTGTAAGAATA
TATTATTAGGAGGCCATAAC

Downstream 100 bases:

>100_bases
AAAGGAGTTTTATAAATGAGAAAACCAATTATCGCAGGAAACTGGAAAATGAACAAAACGGTAAAAGAAGCGAAAGCATT
TGTTTCAAGCTTACCAGAGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MVKKVVSDLELKDKKVLVRADFNVPMKDGNITNDNRIVEALPTIKYIIEQGGKVILFSHLGKVKTEEDKANLSLQPVANR
LSELLDKEVQFVAETRGEKLESAINALQPGDVLLFENTRFEDVDGKKESKNDAELGKYWASLGDVFVNDAFGTAHREHAS
NVGVATHLDTAAGFLMEKEIKFIGGVVENPERPFVAILGGAKVSDKIGVIENLLEVADKVLIGGGMAYTFLKAQGKEIGH
SLLEEDKIDLAKDLMERGKDKLVLPIDAKVTKEFSNDGEIEAVAIDHIPADLQSLDIGPKTVELFGKELEGAKTVVWNGP
MGVFEMSNFAKGTVGVCKAIAELKGATTIIGGGDSAAAAMDLGFADKFTHISTGGGASLEYLEGKELPGIKSISDK

Sequences:

>Translated_396_residues
MVKKVVSDLELKDKKVLVRADFNVPMKDGNITNDNRIVEALPTIKYIIEQGGKVILFSHLGKVKTEEDKANLSLQPVANR
LSELLDKEVQFVAETRGEKLESAINALQPGDVLLFENTRFEDVDGKKESKNDAELGKYWASLGDVFVNDAFGTAHREHAS
NVGVATHLDTAAGFLMEKEIKFIGGVVENPERPFVAILGGAKVSDKIGVIENLLEVADKVLIGGGMAYTFLKAQGKEIGH
SLLEEDKIDLAKDLMERGKDKLVLPIDAKVTKEFSNDGEIEAVAIDHIPADLQSLDIGPKTVELFGKELEGAKTVVWNGP
MGVFEMSNFAKGTVGVCKAIAELKGATTIIGGGDSAAAAMDLGFADKFTHISTGGGASLEYLEGKELPGIKSISDK
>Mature_396_residues
MVKKVVSDLELKDKKVLVRADFNVPMKDGNITNDNRIVEALPTIKYIIEQGGKVILFSHLGKVKTEEDKANLSLQPVANR
LSELLDKEVQFVAETRGEKLESAINALQPGDVLLFENTRFEDVDGKKESKNDAELGKYWASLGDVFVNDAFGTAHREHAS
NVGVATHLDTAAGFLMEKEIKFIGGVVENPERPFVAILGGAKVSDKIGVIENLLEVADKVLIGGGMAYTFLKAQGKEIGH
SLLEEDKIDLAKDLMERGKDKLVLPIDAKVTKEFSNDGEIEAVAIDHIPADLQSLDIGPKTVELFGKELEGAKTVVWNGP
MGVFEMSNFAKGTVGVCKAIAELKGATTIIGGGDSAAAAMDLGFADKFTHISTGGGASLEYLEGKELPGIKSISDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=43.9320388349515, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI31543397, Length=415, Percent_Identity=43.3734939759036, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=43.1122448979592, Blast_Score=298, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=45.873786407767, Blast_Score=336, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6319857, Length=409, Percent_Identity=47.921760391198, Blast_Score=346, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=44.0963855421687, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=43.1372549019608, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_MACCJ (B9EAG8)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559925.1
- GeneID:   7390585
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0522
- OMA:   DCIGDEV
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42593; Mature: 42593

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 119-119 BINDING 156-156 BINDING 206-206 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKVVSDLELKDKKVLVRADFNVPMKDGNITNDNRIVEALPTIKYIIEQGGKVILFSHL
CCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
GKVKTEEDKANLSLQPVANRLSELLDKEVQFVAETRGEKLESAINALQPGDVLLFENTRF
CCCCCCCHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
EDVDGKKESKNDAELGKYWASLGDVFVNDAFGTAHREHASNVGVATHLDTAAGFLMEKEI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHH
KFIGGVVENPERPFVAILGGAKVSDKIGVIENLLEVADKVLIGGGMAYTFLKAQGKEIGH
HHHCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCHHEEHHHHCCHHHHH
SLLEEDKIDLAKDLMERGKDKLVLPIDAKVTKEFSNDGEIEAVAIDHIPADLQSLDIGPK
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCEEEEEECCCCCCHHHCCCCCH
TVELFGKELEGAKTVVWNGPMGVFEMSNFAKGTVGVCKAIAELKGATTIIGGGDSAAAAM
HHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
DLGFADKFTHISTGGGASLEYLEGKELPGIKSISDK
HCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MVKKVVSDLELKDKKVLVRADFNVPMKDGNITNDNRIVEALPTIKYIIEQGGKVILFSHL
CCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
GKVKTEEDKANLSLQPVANRLSELLDKEVQFVAETRGEKLESAINALQPGDVLLFENTRF
CCCCCCCHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
EDVDGKKESKNDAELGKYWASLGDVFVNDAFGTAHREHASNVGVATHLDTAAGFLMEKEI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHH
KFIGGVVENPERPFVAILGGAKVSDKIGVIENLLEVADKVLIGGGMAYTFLKAQGKEIGH
HHHCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCHHEEHHHHCCHHHHH
SLLEEDKIDLAKDLMERGKDKLVLPIDAKVTKEFSNDGEIEAVAIDHIPADLQSLDIGPK
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCEEEEEECCCCCCHHHCCCCCH
TVELFGKELEGAKTVVWNGPMGVFEMSNFAKGTVGVCKAIAELKGATTIIGGGDSAAAAM
HHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
DLGFADKFTHISTGGGASLEYLEGKELPGIKSISDK
HCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA