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The map label for this gene is topB [H]

Identifier: 222150439

GI number: 222150439

Start: 196521

End: 198647

Strand: Direct

Name: topB [H]

Synonym: MCCL_0189

Alternate gene names: 222150439

Gene position: 196521-198647 (Clockwise)

Preceding gene: 222150435

Following gene: 222150440

Centisome position: 9.35

GC content: 38.27

Gene sequence:

>2127_bases
ATGAAATCAGTAGTGTTAGCAGAGAAACCTTCAGTCGCACGTGATATTGCACGTGTACTTAAGTGTAATCAAAAGAAGAA
TGGATATATGGAAAACGACAAATATATCGTCACTTGGGCATTAGGACATTTAGTTACAAATGCTGATCCCGAGCAATATG
ATGCAAAGTATAAACAGTGGGATCTAAATATGCTGCCTATTATACCCGAACGTATGAAGACGGTCGTTATTGGTAAGACC
CGTAAACAATTTAATACAGTGAAAGCACAGATTGAACGTCAAGATGTAAATGAGATTATTATTGCAACTGACGCAGGACG
TGAAGGAGAACTTGTAGCACGCCTTATTATCGAGAAATGTAAAGGGAATAAACCGATGAAGCGTCTCTGGATATCCTCGG
TTACAGACAAAGCAATACTAGACGGCTTTAAACATTTGAAACCAGCGCAGGCGTATCAAGGATTATATGAAGCAGCTATG
GCACGAAGCGAGGCGGATTGGATTGTTGGGATTAATGCAACAAGAGCACTAACAACGAAATATGATGCACAACTTTCTAC
AGGTCGTGTGCAGACGCCGACGCTACAGATGGTCAAGATTAGACAAGATGCTGTGCAGAAATTTCAACCGAAAACATATT
ATAGTATTGAGCTTGTAAGCGGAAGTGATAAGTTCAAGTGGCAAAGTGGGAGTCAGATATTTGATGAATCAAAAGTAGAT
GGACTATTAAAAGACATACAAGGAAAGCAAGCTGTTGTTCAAGATGTAGTCTCTAAAGAAAAGAAACGCTACCCTGGAAA
ACTCTATGATTTAACAAGTTTGCAGCAGGATGCTTATAAGCGTTTCAAATATTCAGCGAAAGAAACATTGAACGCGATGC
AAAGTCTATACGAGACCCATAAATATGTAACATATCCAAGAACAGATTCAAATTACTTAACGACAGATATGGAAAGCTCA
CTTAAGGAACGTGTCGCATCCCTTGGTGCTACACCATATAAAGATCAAGTTGTGAAATTACTAAAGACACACATTAAAGG
TGGTAGTCACTTTATTAACAATGCGAAAGTATCAGATCACCATGCCATTGTTCCGACTGAGATTAGAGCGAACTTAGAAG
CACTTGGAGCAAGAGAAAAGAATATATATTTACTTATTGCAGAACGTTTCCTGAGCAATTTAATGGAGCCCCATATATAT
AATGAAGAAACAATTACTGTACAGATTGGAAAAGAAATATTTACGCTTAAGACTGAGACGACTGTGAAAGCAGGATTTAA
AGCATTGTATGAACAGTCATCCATTGAGCGCCATAAGGTATATCATAAAGGAGAAGTGTTAAGTTCTGTCAGCCTGAAGA
AGACGACCGGTGAAACACAACCCCCAGCTTATTTAAATGAGGGGACATTGCTTAAAGCGATGGAGAACCCGGCGTCTATC
TTTAATATTGATAAGGAGAGTACGACGACATTAAAGTCTGCAGGTGGTATTGGAACAGTGGCTACACGAGCAGATATTAT
TGAAAAGCTTTATAACTTAAATGCGATAGAAAACAATAACGGCAATATTAAAGTGACGAACAAAGGACGTCAGTTATTAG
AACTTGCACCTGATGCACTCAAGTCACCAGAACTTACAGCAGACTGGGAACATAAACTAACACGCATTGAAAAAAATAAG
TATGGAAGAAAGCAATTTATGCGTGAGATGATAGATTTCACAAAAGAAATCATTGATGAAATTAAGTCGAGTGACGAGAA
ATTTAAACATGATAACTTAACGAGTACAGAATGCCCGACTTGCGGTAAGTTTATGTTAGAGAAAAAAACGCGTAACGGCA
AGATGCTTGTATGTCAAGATCCGACTTGCGGTACGAAGAAGAATGTTCAGCGTAAAACAAATGTGCGTTGCCCAGAATGT
AAGAAAAAGCTGACGCTACATGGTAAAGGGCCAAAAGCAATGTACAGTTGTGTATGTGGATTTAGAGAAACGCAGGAACA
GATGGACAAACGCTTCAATTCGAAGAAGACAGGAAAGGTATCGAAAAAAGAAATCAATAAATATATGAAGAAAGAAGAGC
CAATCAATAATCCTTTTGCCGATGCACTCAAAAACTTAAATTTATAA

Upstream 100 bases:

>100_bases
CTTAAGTAAAGCATACAAGATAGTACCTAAAATCTCAATTTAATCATATGAAATATATTCTGTTATAATAAAGATTATTA
AAGAATAGAGGACGGATATT

Downstream 100 bases:

>100_bases
GTAGACTAAATATCTATTGTTCAATACTTCACAAATTGTTAAAATAGAAGTGAATAAAATAGGGGGTGTTTCTATGGAAA
ACGTAAAACAAAAGAAGAAT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MKSVVLAEKPSVARDIARVLKCNQKKNGYMENDKYIVTWALGHLVTNADPEQYDAKYKQWDLNMLPIIPERMKTVVIGKT
RKQFNTVKAQIERQDVNEIIIATDAGREGELVARLIIEKCKGNKPMKRLWISSVTDKAILDGFKHLKPAQAYQGLYEAAM
ARSEADWIVGINATRALTTKYDAQLSTGRVQTPTLQMVKIRQDAVQKFQPKTYYSIELVSGSDKFKWQSGSQIFDESKVD
GLLKDIQGKQAVVQDVVSKEKKRYPGKLYDLTSLQQDAYKRFKYSAKETLNAMQSLYETHKYVTYPRTDSNYLTTDMESS
LKERVASLGATPYKDQVVKLLKTHIKGGSHFINNAKVSDHHAIVPTEIRANLEALGAREKNIYLLIAERFLSNLMEPHIY
NEETITVQIGKEIFTLKTETTVKAGFKALYEQSSIERHKVYHKGEVLSSVSLKKTTGETQPPAYLNEGTLLKAMENPASI
FNIDKESTTTLKSAGGIGTVATRADIIEKLYNLNAIENNNGNIKVTNKGRQLLELAPDALKSPELTADWEHKLTRIEKNK
YGRKQFMREMIDFTKEIIDEIKSSDEKFKHDNLTSTECPTCGKFMLEKKTRNGKMLVCQDPTCGTKKNVQRKTNVRCPEC
KKKLTLHGKGPKAMYSCVCGFRETQEQMDKRFNSKKTGKVSKKEINKYMKKEEPINNPFADALKNLNL

Sequences:

>Translated_708_residues
MKSVVLAEKPSVARDIARVLKCNQKKNGYMENDKYIVTWALGHLVTNADPEQYDAKYKQWDLNMLPIIPERMKTVVIGKT
RKQFNTVKAQIERQDVNEIIIATDAGREGELVARLIIEKCKGNKPMKRLWISSVTDKAILDGFKHLKPAQAYQGLYEAAM
ARSEADWIVGINATRALTTKYDAQLSTGRVQTPTLQMVKIRQDAVQKFQPKTYYSIELVSGSDKFKWQSGSQIFDESKVD
GLLKDIQGKQAVVQDVVSKEKKRYPGKLYDLTSLQQDAYKRFKYSAKETLNAMQSLYETHKYVTYPRTDSNYLTTDMESS
LKERVASLGATPYKDQVVKLLKTHIKGGSHFINNAKVSDHHAIVPTEIRANLEALGAREKNIYLLIAERFLSNLMEPHIY
NEETITVQIGKEIFTLKTETTVKAGFKALYEQSSIERHKVYHKGEVLSSVSLKKTTGETQPPAYLNEGTLLKAMENPASI
FNIDKESTTTLKSAGGIGTVATRADIIEKLYNLNAIENNNGNIKVTNKGRQLLELAPDALKSPELTADWEHKLTRIEKNK
YGRKQFMREMIDFTKEIIDEIKSSDEKFKHDNLTSTECPTCGKFMLEKKTRNGKMLVCQDPTCGTKKNVQRKTNVRCPEC
KKKLTLHGKGPKAMYSCVCGFRETQEQMDKRFNSKKTGKVSKKEINKYMKKEEPINNPFADALKNLNL
>Mature_708_residues
MKSVVLAEKPSVARDIARVLKCNQKKNGYMENDKYIVTWALGHLVTNADPEQYDAKYKQWDLNMLPIIPERMKTVVIGKT
RKQFNTVKAQIERQDVNEIIIATDAGREGELVARLIIEKCKGNKPMKRLWISSVTDKAILDGFKHLKPAQAYQGLYEAAM
ARSEADWIVGINATRALTTKYDAQLSTGRVQTPTLQMVKIRQDAVQKFQPKTYYSIELVSGSDKFKWQSGSQIFDESKVD
GLLKDIQGKQAVVQDVVSKEKKRYPGKLYDLTSLQQDAYKRFKYSAKETLNAMQSLYETHKYVTYPRTDSNYLTTDMESS
LKERVASLGATPYKDQVVKLLKTHIKGGSHFINNAKVSDHHAIVPTEIRANLEALGAREKNIYLLIAERFLSNLMEPHIY
NEETITVQIGKEIFTLKTETTVKAGFKALYEQSSIERHKVYHKGEVLSSVSLKKTTGETQPPAYLNEGTLLKAMENPASI
FNIDKESTTTLKSAGGIGTVATRADIIEKLYNLNAIENNNGNIKVTNKGRQLLELAPDALKSPELTADWEHKLTRIEKNK
YGRKQFMREMIDFTKEIIDEIKSSDEKFKHDNLTSTECPTCGKFMLEKKTRNGKMLVCQDPTCGTKKNVQRKTNVRCPEC
KKKLTLHGKGPKAMYSCVCGFRETQEQMDKRFNSKKTGKVSKKEINKYMKKEEPINNPFADALKNLNL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=671, Percent_Identity=25.4843517138599, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI10835218, Length=803, Percent_Identity=23.5367372353674, Blast_Score=134, Evalue=4e-31,
Organism=Escherichia coli, GI1788061, Length=620, Percent_Identity=30, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1787529, Length=745, Percent_Identity=24.5637583892617, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32563869, Length=681, Percent_Identity=23.3480176211454, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=635, Percent_Identity=23.9370078740157, Blast_Score=106, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=588, Percent_Identity=23.469387755102, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24585251, Length=736, Percent_Identity=23.9130434782609, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24640096, Length=701, Percent_Identity=22.1112696148359, Blast_Score=109, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80424; Mature: 80424

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVVLAEKPSVARDIARVLKCNQKKNGYMENDKYIVTWALGHLVTNADPEQYDAKYKQW
CCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHCCCCCHHHCCHHHHC
DLNMLPIIPERMKTVVIGKTRKQFNTVKAQIERQDVNEIIIATDAGREGELVARLIIEKC
CCCCCCCCHHHHHEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KGNKPMKRLWISSVTDKAILDGFKHLKPAQAYQGLYEAAMARSEADWIVGINATRALTTK
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHEEEH
YDAQLSTGRVQTPTLQMVKIRQDAVQKFQPKTYYSIELVSGSDKFKWQSGSQIFDESKVD
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
GLLKDIQGKQAVVQDVVSKEKKRYPGKLYDLTSLQQDAYKRFKYSAKETLNAMQSLYETH
HHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYVTYPRTDSNYLTTDMESSLKERVASLGATPYKDQVVKLLKTHIKGGSHFINNAKVSDH
CEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCC
HAIVPTEIRANLEALGAREKNIYLLIAERFLSNLMEPHIYNEETITVQIGKEIFTLKTET
CCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECCH
TVKAGFKALYEQSSIERHKVYHKGEVLSSVSLKKTTGETQPPAYLNEGTLLKAMENPASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEHHHCCCHHE
FNIDKESTTTLKSAGGIGTVATRADIIEKLYNLNAIENNNGNIKVTNKGRQLLELAPDAL
EECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEECCCHHHHHHCHHHH
KSPELTADWEHKLTRIEKNKYGRKQFMREMIDFTKEIIDEIKSSDEKFKHDNLTSTECPT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
CGKFMLEKKTRNGKMLVCQDPTCGTKKNVQRKTNVRCPECKKKLTLHGKGPKAMYSCVCG
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHCCCCCCCHHCCEEECCCCHHHHHHHHHC
FRETQEQMDKRFNSKKTGKVSKKEINKYMKKEEPINNPFADALKNLNL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKSVVLAEKPSVARDIARVLKCNQKKNGYMENDKYIVTWALGHLVTNADPEQYDAKYKQW
CCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHCCCCCHHHCCHHHHC
DLNMLPIIPERMKTVVIGKTRKQFNTVKAQIERQDVNEIIIATDAGREGELVARLIIEKC
CCCCCCCCHHHHHEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
KGNKPMKRLWISSVTDKAILDGFKHLKPAQAYQGLYEAAMARSEADWIVGINATRALTTK
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHEEEH
YDAQLSTGRVQTPTLQMVKIRQDAVQKFQPKTYYSIELVSGSDKFKWQSGSQIFDESKVD
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
GLLKDIQGKQAVVQDVVSKEKKRYPGKLYDLTSLQQDAYKRFKYSAKETLNAMQSLYETH
HHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYVTYPRTDSNYLTTDMESSLKERVASLGATPYKDQVVKLLKTHIKGGSHFINNAKVSDH
CEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCC
HAIVPTEIRANLEALGAREKNIYLLIAERFLSNLMEPHIYNEETITVQIGKEIFTLKTET
CCCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECCH
TVKAGFKALYEQSSIERHKVYHKGEVLSSVSLKKTTGETQPPAYLNEGTLLKAMENPASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEHHHCCCHHE
FNIDKESTTTLKSAGGIGTVATRADIIEKLYNLNAIENNNGNIKVTNKGRQLLELAPDAL
EECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEECCCHHHHHHCHHHH
KSPELTADWEHKLTRIEKNKYGRKQFMREMIDFTKEIIDEIKSSDEKFKHDNLTSTECPT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
CGKFMLEKKTRNGKMLVCQDPTCGTKKNVQRKTNVRCPECKKKLTLHGKGPKAMYSCVCG
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHCCCCCCCHHCCEEECCCCHHHHHHHHHC
FRETQEQMDKRFNSKKTGKVSKKEINKYMKKEEPINNPFADALKNLNL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA