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The map label for this gene is nupG [H]

Identifier: 222150411

GI number: 222150411

Start: 165191

End: 166396

Strand: Direct

Name: nupG [H]

Synonym: MCCL_0161

Alternate gene names: 222150411

Gene position: 165191-166396 (Clockwise)

Preceding gene: 222150409

Following gene: 222150412

Centisome position: 7.86

GC content: 35.82

Gene sequence:

>1206_bases
ATGAATACTTATTTAATATTGAACATTATTGGTATCTTAGTTTTTATTCTTGTTGCATTCTTGTTCTCTCGCGATAGAAA
GAACATCAACTGGAAATCAGTCGTAATTATGTTAGTACTTAATTTATTTTTAGCTTGGTTCTTTACGCAATTCCCATACG
GCAGAATTGGCGTTCAAAAAGTAGCGGATGCTTTTAGTTGGATGTTGCAAGCTGCATATGTTGGTATCGGAATGCCCTTT
GCAAGCTTCACGGCACCTGGTCCTGGTAAGATGGATATGGCTGTTTCAGCTTTACTACCTATTCTTTTCGTAGTTCCTCT
GTTTGATATTTTAATGTATATCGGAGTATTACCATTTATCATTAAATGGATGGGCTGGGTAATCGGTAAGGTTACAGGAC
AACCGAAATTTGAGTCGTTTTATTCTATTGAAATGATGGTTTTAGGAAATACAGAAGCATTAGCGGTATCGAATGCGCAA
GTAAAGCAAATGAACGAAGCGCGTGTCCTTACCATTGCATTAATGTCTATGAGCTGTATCTCAGGATCTATCGTTGGTGC
ATATATTTCTATGATGCCTGGGGAATTGGTATTAACTGCAATTCCATTAAATATTATCAATGCTATTATTATCGCAACAA
TCTTAAATCCTGTTAAGTTAACACCTGAAGAAGACTATGTTGTTGAAATTAAAGCTGAGACACGTCAGCCATTCTTCTCA
TTCTTAGGAGATTCAGTACTTGGTGCAGGAAAGTTAATCTTAATTATTATTGCATTTTTAATCGCATTTGTCTCATTGGC
ACATTTTATTGACATGATATTAGGACTATTCCCGGGTAATTTAACATTAAGTATTATCTTAGGATGGATAATGGCACCAT
TTGCCATGCTTTTAGGCTTACCTTGGGCAGAAGCGAAAGTTGTCGGTGGTCATATGGCAACAAAGGTTATCACTAATGAA
TTTGTTGTTATGCTGGGAGTTAAAGATCAACTGAAATCTTTCTCGCCGCATATGACAGCAGTACTTGTAACATTCTTGAT
TTCATTCGCTAACTTCTCAACTATTGGTATGATTATTGGGACGTTAAAAGGAATTGTGAACGAAAAAACTTCTGATTTTG
TAAGTAAATATGTACCGATGATGTTATTGTCAGGTATTCTAGTATCGTTACTATCTGCAGCGTTTGTTGGATTATTTACT
TGGTAA

Upstream 100 bases:

>100_bases
TTAATGAACAAATGTTCGTTTTTTATTGGAAAAAAGTGTGCTTAAAACAAAAAAACCATGGTATAATCATGAATGTAAAA
TTTAATTAAAGGTGGAAAAG

Downstream 100 bases:

>100_bases
GAAAATTTAGGGCTGAGACATTAAGTCTCAGTCCTTTTTTTATTTGTTTCTTTATGTTTAAGGATCTAATTATCGTAAAT
ATATAAAGTAAAGCTTCTGC

Product: pyrimidine nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNTYLILNIIGILVFILVAFLFSRDRKNINWKSVVIMLVLNLFLAWFFTQFPYGRIGVQKVADAFSWMLQAAYVGIGMPF
ASFTAPGPGKMDMAVSALLPILFVVPLFDILMYIGVLPFIIKWMGWVIGKVTGQPKFESFYSIEMMVLGNTEALAVSNAQ
VKQMNEARVLTIALMSMSCISGSIVGAYISMMPGELVLTAIPLNIINAIIIATILNPVKLTPEEDYVVEIKAETRQPFFS
FLGDSVLGAGKLILIIIAFLIAFVSLAHFIDMILGLFPGNLTLSIILGWIMAPFAMLLGLPWAEAKVVGGHMATKVITNE
FVVMLGVKDQLKSFSPHMTAVLVTFLISFANFSTIGMIIGTLKGIVNEKTSDFVSKYVPMMLLSGILVSLLSAAFVGLFT
W

Sequences:

>Translated_401_residues
MNTYLILNIIGILVFILVAFLFSRDRKNINWKSVVIMLVLNLFLAWFFTQFPYGRIGVQKVADAFSWMLQAAYVGIGMPF
ASFTAPGPGKMDMAVSALLPILFVVPLFDILMYIGVLPFIIKWMGWVIGKVTGQPKFESFYSIEMMVLGNTEALAVSNAQ
VKQMNEARVLTIALMSMSCISGSIVGAYISMMPGELVLTAIPLNIINAIIIATILNPVKLTPEEDYVVEIKAETRQPFFS
FLGDSVLGAGKLILIIIAFLIAFVSLAHFIDMILGLFPGNLTLSIILGWIMAPFAMLLGLPWAEAKVVGGHMATKVITNE
FVVMLGVKDQLKSFSPHMTAVLVTFLISFANFSTIGMIIGTLKGIVNEKTSDFVSKYVPMMLLSGILVSLLSAAFVGLFT
W
>Mature_401_residues
MNTYLILNIIGILVFILVAFLFSRDRKNINWKSVVIMLVLNLFLAWFFTQFPYGRIGVQKVADAFSWMLQAAYVGIGMPF
ASFTAPGPGKMDMAVSALLPILFVVPLFDILMYIGVLPFIIKWMGWVIGKVTGQPKFESFYSIEMMVLGNTEALAVSNAQ
VKQMNEARVLTIALMSMSCISGSIVGAYISMMPGELVLTAIPLNIINAIIIATILNPVKLTPEEDYVVEIKAETRQPFFS
FLGDSVLGAGKLILIIIAFLIAFVSLAHFIDMILGLFPGNLTLSIILGWIMAPFAMLLGLPWAEAKVVGGHMATKVITNE
FVVMLGVKDQLKSFSPHMTAVLVTFLISFANFSTIGMIIGTLKGIVNEKTSDFVSKYVPMMLLSGILVSLLSAAFVGLFT
W

Specific function: Involved in the uptake of the purine ribonucleosides inosine and guanosine [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=426, Percent_Identity=25.1173708920188, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI11545853, Length=421, Percent_Identity=24.7030878859857, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI227116277, Length=425, Percent_Identity=23.5294117647059, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788737, Length=394, Percent_Identity=34.010152284264, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1788488, Length=420, Percent_Identity=27.6190476190476, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788485, Length=420, Percent_Identity=27.1428571428571, Blast_Score=121, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71991794, Length=398, Percent_Identity=24.3718592964824, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560276, Length=394, Percent_Identity=24.1116751269036, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281360430, Length=413, Percent_Identity=23.9709443099274, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45552517, Length=407, Percent_Identity=21.8673218673219, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921868, Length=407, Percent_Identity=21.8673218673219, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45552519, Length=407, Percent_Identity=21.8673218673219, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43957; Mature: 43957

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTYLILNIIGILVFILVAFLFSRDRKNINWKSVVIMLVLNLFLAWFFTQFPYGRIGVQK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
VADAFSWMLQAAYVGIGMPFASFTAPGPGKMDMAVSALLPILFVVPLFDILMYIGVLPFI
HHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKWMGWVIGKVTGQPKFESFYSIEMMVLGNTEALAVSNAQVKQMNEARVLTIALMSMSCI
HHHHHHHHHHCCCCCCCCHHEEEEEEEECCCCEEEECCHHHHHHCCHHHHHHHHHHHHHH
SGSIVGAYISMMPGELVLTAIPLNIINAIIIATILNPVKLTPEEDYVVEIKAETRQPFFS
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
FLGDSVLGAGKLILIIIAFLIAFVSLAHFIDMILGLFPGNLTLSIILGWIMAPFAMLLGL
HHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
PWAEAKVVGGHMATKVITNEFVVMLGVKDQLKSFSPHMTAVLVTFLISFANFSTIGMIIG
CCHHHHHHCHHHHHHHHHHHHEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLKGIVNEKTSDFVSKYVPMMLLSGILVSLLSAAFVGLFTW
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTYLILNIIGILVFILVAFLFSRDRKNINWKSVVIMLVLNLFLAWFFTQFPYGRIGVQK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
VADAFSWMLQAAYVGIGMPFASFTAPGPGKMDMAVSALLPILFVVPLFDILMYIGVLPFI
HHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKWMGWVIGKVTGQPKFESFYSIEMMVLGNTEALAVSNAQVKQMNEARVLTIALMSMSCI
HHHHHHHHHHCCCCCCCCHHEEEEEEEECCCCEEEECCHHHHHHCCHHHHHHHHHHHHHH
SGSIVGAYISMMPGELVLTAIPLNIINAIIIATILNPVKLTPEEDYVVEIKAETRQPFFS
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
FLGDSVLGAGKLILIIIAFLIAFVSLAHFIDMILGLFPGNLTLSIILGWIMAPFAMLLGL
HHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
PWAEAKVVGGHMATKVITNEFVVMLGVKDQLKSFSPHMTAVLVTFLISFANFSTIGMIIG
CCHHHHHHCHHHHHHHHHHHHEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLKGIVNEKTSDFVSKYVPMMLLSGILVSLLSAAFVGLFTW
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]