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The map label for this gene is bioB

Identifier: 222111048

GI number: 222111048

Start: 1976418

End: 1977470

Strand: Direct

Name: bioB

Synonym: Dtpsy_1855

Alternate gene names: 222111048

Gene position: 1976418-1977470 (Clockwise)

Preceding gene: 222111047

Following gene: 222111054

Centisome position: 52.06

GC content: 64.1

Gene sequence:

>1053_bases
ATGACCTCAGCCACGACAGCCCCGCATACAACCCAGACCCTGCAGTTTCATCCTCGCGTTGCCGAGAGTGTTGCGACGCA
GCGAGGGGAGGGCTGGAGCATTCAAGCGGTACAGGAGCTCCTCGATCTTCCATTCATGGAACTGCTCTGGCGCGCGCAGG
CCACGCACCGCGCACATTGGCCCCAGGGCGACATCGAATTGGCCACGCTGCTGTCCGTGAAAACAGGCGGCTGTCCGGAA
AACTGCGGCTACTGCCCCCAGTCGGCGGAGTTCGATACCGGCGTGAAGGCCGAAAAACTCATGAGCGTGCAGGAGGTAAC
CCAGGCAGCGCAAGCCGCCAAGGACGCCGGCGCGACGCGCTTTTGCATGGGCGCCGCCTGGCGAGCACCGAAGGACCGCG
ACATCGAGAAGATGAGCGAGCTGATCACCGCCGTGAAGGACCTCGGCCTGCAGACCTGCGCCACGCTGGGCATGTTGCAG
TCGCACCAGGCCCTGGCGCTGAAGGATGCCGGACTGGACTACTACAACCACAACCTGGACACGGCCCCCGAGTACTACAG
CGACGTGGTCAGCACGCGCCAGTACCAGGACCGGCTGGATACGCTGCGCCATGTGCGCGACGCAGGCATCAACGTGTGCT
GCGGCGGCATCGTTGGCATGGGCGAAGCGCCGGTGCACCGCGCGGGTCTCATCGCGCAGTTGGCCAACCTAAACCCGTAT
CCGGAGTCGGTGCCCATCAACAGCCTGGTGCGTGTTGCCGGAACACCGTTGGCCAACAGCGAGCCGGTGGACCCGCTGGA
CTTCGTGCGCGTGATCGCGGTGGCCCGCATCACCATGCCCAAGGCTCGCGTACGCTTGTCCGCCGGCCGCCAGCAGCTTG
GCGACGCGGTGCAGGCTTTGTGCTTCTTGGCGGGTGCCAATTCCATCTTCTATGGCGACAAGCTGCTCGTGACGAGCAAC
CCGGATGTAGAGGCCGACACCCAACTGCTGGCCAAACTGGGGTTGAAGGGTACGCCCAACCAAACCACCACAGAAACTGC
TTGCGGTGCTTAA

Upstream 100 bases:

>100_bases
CATCCTGCTTTTTGAACGCTAAACCTTCACAGACCACTCAAGCCTTTACACAATCAGGAGCAGCGCCCAGCAGTGGCGAC
CGCAGAACCGGAGAAATTTG

Downstream 100 bases:

>100_bases
TCTGCATGCGCTGTTTTGTGCAGAGGATGCGCGCTTTGAAGAATCAGGGTGCCACCAGAGCTGCGTGCGGTTTTTCGGTA
CGCCATCTGTAGGGGCCACA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTSATTAPHTTQTLQFHPRVAESVATQRGEGWSIQAVQELLDLPFMELLWRAQATHRAHWPQGDIELATLLSVKTGGCPE
NCGYCPQSAEFDTGVKAEKLMSVQEVTQAAQAAKDAGATRFCMGAAWRAPKDRDIEKMSELITAVKDLGLQTCATLGMLQ
SHQALALKDAGLDYYNHNLDTAPEYYSDVVSTRQYQDRLDTLRHVRDAGINVCCGGIVGMGEAPVHRAGLIAQLANLNPY
PESVPINSLVRVAGTPLANSEPVDPLDFVRVIAVARITMPKARVRLSAGRQQLGDAVQALCFLAGANSIFYGDKLLVTSN
PDVEADTQLLAKLGLKGTPNQTTTETACGA

Sequences:

>Translated_350_residues
MTSATTAPHTTQTLQFHPRVAESVATQRGEGWSIQAVQELLDLPFMELLWRAQATHRAHWPQGDIELATLLSVKTGGCPE
NCGYCPQSAEFDTGVKAEKLMSVQEVTQAAQAAKDAGATRFCMGAAWRAPKDRDIEKMSELITAVKDLGLQTCATLGMLQ
SHQALALKDAGLDYYNHNLDTAPEYYSDVVSTRQYQDRLDTLRHVRDAGINVCCGGIVGMGEAPVHRAGLIAQLANLNPY
PESVPINSLVRVAGTPLANSEPVDPLDFVRVIAVARITMPKARVRLSAGRQQLGDAVQALCFLAGANSIFYGDKLLVTSN
PDVEADTQLLAKLGLKGTPNQTTTETACGA
>Mature_349_residues
TSATTAPHTTQTLQFHPRVAESVATQRGEGWSIQAVQELLDLPFMELLWRAQATHRAHWPQGDIELATLLSVKTGGCPEN
CGYCPQSAEFDTGVKAEKLMSVQEVTQAAQAAKDAGATRFCMGAAWRAPKDRDIEKMSELITAVKDLGLQTCATLGMLQS
HQALALKDAGLDYYNHNLDTAPEYYSDVVSTRQYQDRLDTLRHVRDAGINVCCGGIVGMGEAPVHRAGLIAQLANLNPYP
ESVPINSLVRVAGTPLANSEPVDPLDFVRVIAVARITMPKARVRLSAGRQQLGDAVQALCFLAGANSIFYGDKLLVTSNP
DVEADTQLLAKLGLKGTPNQTTTETACGA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=311, Percent_Identity=57.2347266881029, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321725, Length=344, Percent_Identity=45.3488372093023, Blast_Score=308, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ACIET (B9MJH4)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002553312.1
- GeneID:   7382216
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1855
- ProtClustDB:   CLSK2321419
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37658; Mature: 37527

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATTAPHTTQTLQFHPRVAESVATQRGEGWSIQAVQELLDLPFMELLWRAQATHRAHW
CCCCCCCCCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
PQGDIELATLLSVKTGGCPENCGYCPQSAEFDTGVKAEKLMSVQEVTQAAQAAKDAGATR
CCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
FCMGAAWRAPKDRDIEKMSELITAVKDLGLQTCATLGMLQSHQALALKDAGLDYYNHNLD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHCCCCC
TAPEYYSDVVSTRQYQDRLDTLRHVRDAGINVCCGGIVGMGEAPVHRAGLIAQLANLNPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECCCCCCHHHHHHHHHHHCCCCC
PESVPINSLVRVAGTPLANSEPVDPLDFVRVIAVARITMPKARVRLSAGRQQLGDAVQAL
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
CFLAGANSIFYGDKLLVTSNPDVEADTQLLAKLGLKGTPNQTTTETACGA
HHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSATTAPHTTQTLQFHPRVAESVATQRGEGWSIQAVQELLDLPFMELLWRAQATHRAHW
CCCCCCCCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCC
PQGDIELATLLSVKTGGCPENCGYCPQSAEFDTGVKAEKLMSVQEVTQAAQAAKDAGATR
CCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
FCMGAAWRAPKDRDIEKMSELITAVKDLGLQTCATLGMLQSHQALALKDAGLDYYNHNLD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHCCCCC
TAPEYYSDVVSTRQYQDRLDTLRHVRDAGINVCCGGIVGMGEAPVHRAGLIAQLANLNPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECCCCCCHHHHHHHHHHHCCCCC
PESVPINSLVRVAGTPLANSEPVDPLDFVRVIAVARITMPKARVRLSAGRQQLGDAVQAL
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
CFLAGANSIFYGDKLLVTSNPDVEADTQLLAKLGLKGTPNQTTTETACGA
HHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA