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The map label for this gene is lhgO [H]

Identifier: 222110840

GI number: 222110840

Start: 1737333

End: 1738448

Strand: Direct

Name: lhgO [H]

Synonym: Dtpsy_1646

Alternate gene names: 222110840

Gene position: 1737333-1738448 (Clockwise)

Preceding gene: 222110839

Following gene: 222110841

Centisome position: 45.76

GC content: 65.95

Gene sequence:

>1116_bases
ATGAGTGAGATGGTCGATTGCGTCGTGGTGGGCGCGGGGGTGGTGGGGCTGGCGGTGGCGCGCGCATTGGCGCTGCAAGG
GCGCGAGGTCTGGCTGCTGGAAGCGGCCAATGCCATTGGCACCGAGACGAGTTCGCGCAACAGCGAGGTGATCCACGCCG
GCATCTATTACCCGCAGGGTTCGCTCAAGGCGCGCTTGTGCGTCCAAGGCAAGGCCATGCTGTACGACTACTGCAGCGAG
CGCGGCGTGCCCCATAGTCGCTGCGGCAAGCTCATCGTCGCCACGTCGACCGAACAGCAGAACGCGCTGACGGGCATCAG
AGCGCGCGCGCAGGCCAATGGCGTGGATGACCTGCAATGGCTCTCGCGGGAGGAAGCACGTGCGCTGGAGCCCGAACTGG
AATGTGTGGCAGCGCTGCTCTCGCCCAGTACCGGCATCGTGGACAGCCATGCCCTCATGCTGGCGTTGCTGGGCGACCTG
GAGAACGCGGGTGGTGTTCTGGCATTGAATTCGCCTCTGGCCAAGGCCCATTGCGCGCCACAAGCTATCCATTTGGAAGC
AATGGATGGCACGTGCATCAGTGCGCGCACGGTGGTCAACGCGGCCGGTTTGCATGCGCCAGTCCTCGCGCGCCAATTCG
AGGGGTTGTCCGCGGTGCATGTACCGCAGGCGGCCTATGCCAAAGGCAACTATTTCACGTTGTCCGGCCGGGCACCCTTC
AGCCATCTCATCTATCCCGTCCCGGAGGCGGCGGGGCTGGGCGTGCACCTGACGCTCGACCTGGGGGGGCAGGCCAAGTT
CGGCCCCGACGTGCAATGGGTGGAAGGCCCCGAGGACCTGGTGGTGGATGCGAGCCGCTCCGAGGCCTTTTATGCCGAGG
TGCGCAAGTACTGGCCCGGCCTGCGCGACGGTGCATTGGCGGCGGGCTACGCCGGCATACGCCCGAAGATCCACGGCCCC
CATGAGCCCGCGGCGGACTTCCTGATCCAGGGGCCGCGCGATCACGGAGTCTCGGGGCTGGTCAACCTGTTTGGCATCGA
ATCACCTGGGCTGACCAGCGCGTTGGCAATCGGTGCGCACGTGGTCGGTCTGCTGCACCAAGACGCCGTGTCTTGA

Upstream 100 bases:

>100_bases
TGCTGGTGCTGGAAGGCGGCTACGCAGCGGCGGCGCTGGGAACGAATGCGGTGAATGTGCTCGAGGGTTTCGAGCAGGCA
TGACAACGGAGGCATGAGCG

Downstream 100 bases:

>100_bases
TGCAACGCGGTCATTTTGTGATAGCGGCCATGCCCTGCGGCGCGAGCACTGGGCTAAGATGAGATACCCGCCTACATGGT
TTGCCATGTACGGCGTATTA

Product: 2-hydroxyglutarate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSEMVDCVVVGAGVVGLAVARALALQGREVWLLEAANAIGTETSSRNSEVIHAGIYYPQGSLKARLCVQGKAMLYDYCSE
RGVPHSRCGKLIVATSTEQQNALTGIRARAQANGVDDLQWLSREEARALEPELECVAALLSPSTGIVDSHALMLALLGDL
ENAGGVLALNSPLAKAHCAPQAIHLEAMDGTCISARTVVNAAGLHAPVLARQFEGLSAVHVPQAAYAKGNYFTLSGRAPF
SHLIYPVPEAAGLGVHLTLDLGGQAKFGPDVQWVEGPEDLVVDASRSEAFYAEVRKYWPGLRDGALAAGYAGIRPKIHGP
HEPAADFLIQGPRDHGVSGLVNLFGIESPGLTSALAIGAHVVGLLHQDAVS

Sequences:

>Translated_371_residues
MSEMVDCVVVGAGVVGLAVARALALQGREVWLLEAANAIGTETSSRNSEVIHAGIYYPQGSLKARLCVQGKAMLYDYCSE
RGVPHSRCGKLIVATSTEQQNALTGIRARAQANGVDDLQWLSREEARALEPELECVAALLSPSTGIVDSHALMLALLGDL
ENAGGVLALNSPLAKAHCAPQAIHLEAMDGTCISARTVVNAAGLHAPVLARQFEGLSAVHVPQAAYAKGNYFTLSGRAPF
SHLIYPVPEAAGLGVHLTLDLGGQAKFGPDVQWVEGPEDLVVDASRSEAFYAEVRKYWPGLRDGALAAGYAGIRPKIHGP
HEPAADFLIQGPRDHGVSGLVNLFGIESPGLTSALAIGAHVVGLLHQDAVS
>Mature_370_residues
SEMVDCVVVGAGVVGLAVARALALQGREVWLLEAANAIGTETSSRNSEVIHAGIYYPQGSLKARLCVQGKAMLYDYCSER
GVPHSRCGKLIVATSTEQQNALTGIRARAQANGVDDLQWLSREEARALEPELECVAALLSPSTGIVDSHALMLALLGDLE
NAGGVLALNSPLAKAHCAPQAIHLEAMDGTCISARTVVNAAGLHAPVLARQFEGLSAVHVPQAAYAKGNYFTLSGRAPFS
HLIYPVPEAAGLGVHLTLDLGGQAKFGPDVQWVEGPEDLVVDASRSEAFYAEVRKYWPGLRDGALAAGYAGIRPKIHGPH
EPAADFLIQGPRDHGVSGLVNLFGIESPGLTSALAIGAHVVGLLHQDAVS

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=271, Percent_Identity=32.8413284132841, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI87082148, Length=376, Percent_Identity=28.7234042553192, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17565764, Length=278, Percent_Identity=34.8920863309352, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24585081, Length=267, Percent_Identity=34.4569288389513, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 38877; Mature: 38746

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMVDCVVVGAGVVGLAVARALALQGREVWLLEAANAIGTETSSRNSEVIHAGIYYPQG
CCCHHEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHCCCCCCCCCCEEEEEEECCCC
SLKARLCVQGKAMLYDYCSERGVPHSRCGKLIVATSTEQQNALTGIRARAQANGVDDLQW
CCEEEEEECCCHHHHHHHHHCCCCHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHH
LSREEARALEPELECVAALLSPSTGIVDSHALMLALLGDLENAGGVLALNSPLAKAHCAP
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCC
QAIHLEAMDGTCISARTVVNAAGLHAPVLARQFEGLSAVHVPQAAYAKGNYFTLSGRAPF
CEEEEEECCCCEEEHHHHHHHCCCHHHHHHHHHCCCCEEECCCHHHCCCCEEEEECCCCH
SHLIYPVPEAAGLGVHLTLDLGGQAKFGPDVQWVEGPEDLVVDASRSEAFYAEVRKYWPG
HHCEEECCCCCCCCEEEEEEECCCCCCCCCCEEECCCHHEEEECCCCHHHHHHHHHHCCC
LRDGALAAGYAGIRPKIHGPHEPAADFLIQGPRDHGVSGLVNLFGIESPGLTSALAIGAH
CCCCCHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
VVGLLHQDAVS
HHHHHHHHCCC
>Mature Secondary Structure 
SEMVDCVVVGAGVVGLAVARALALQGREVWLLEAANAIGTETSSRNSEVIHAGIYYPQG
CCHHEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHCCCCCCCCCCEEEEEEECCCC
SLKARLCVQGKAMLYDYCSERGVPHSRCGKLIVATSTEQQNALTGIRARAQANGVDDLQW
CCEEEEEECCCHHHHHHHHHCCCCHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHH
LSREEARALEPELECVAALLSPSTGIVDSHALMLALLGDLENAGGVLALNSPLAKAHCAP
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCC
QAIHLEAMDGTCISARTVVNAAGLHAPVLARQFEGLSAVHVPQAAYAKGNYFTLSGRAPF
CEEEEEECCCCEEEHHHHHHHCCCHHHHHHHHHCCCCEEECCCHHHCCCCEEEEECCCCH
SHLIYPVPEAAGLGVHLTLDLGGQAKFGPDVQWVEGPEDLVVDASRSEAFYAEVRKYWPG
HHCEEECCCCCCCCEEEEEEECCCCCCCCCCEEECCCHHEEEECCCCHHHHHHHHHHCCC
LRDGALAAGYAGIRPKIHGPHEPAADFLIQGPRDHGVSGLVNLFGIESPGLTSALAIGAH
CCCCCHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
VVGLLHQDAVS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]