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The map label for this gene is cysU [H]

Identifier: 222110717

GI number: 222110717

Start: 1602346

End: 1603197

Strand: Direct

Name: cysU [H]

Synonym: Dtpsy_1520

Alternate gene names: 222110717

Gene position: 1602346-1603197 (Clockwise)

Preceding gene: 222110716

Following gene: 222110718

Centisome position: 42.21

GC content: 63.62

Gene sequence:

>852_bases
ATGAGTGGCAAGAAAAGCGCCCCCAGGCGAGCCAAGAGGGTATTGCCCGGCTTTGGCCTCACGCTGGGCTACACGCTGTT
TTACCTGAGCATCATCGTGCTGATCCCGCTGCTGGCGCTGCTGCTCAAGACGTTCACCATGACCTGGCCGCAGTTCTGGG
AAGCGGTGGCGTCGCCGCGCGTGCTGGCGTCGTACCGCCTCACGTTCGGTGCCTCGTTCATCGCCGCCTGCGTGAACTTG
GTGTTCGGCCTGCTCATCGCCTGGGTGCTGGTGCGCTACAAGTTCCCGGGCAAGAAGATCGTGGATGCACTGGTGGACCT
GCCGTTCGCGCTGCCCACAGCGGTCGCCGGTATTTCGCTCACCGCACTGCTCGCGGGCAATGGCTGGGTGGGGCAGTACC
TGGAGCCGCTGGGCATCCAGCTCGCATTCAACCCCAACGGCATTGTGATTGCGCTGATTTTCATCGGCCTGCCATTCGTG
GTGCGCACGGTGCAGCCAGTGCTGGAAGACAGCGAGAAGGAGTTGGAAGAAGCGGCCACCAGCCTGGGCGCCACGCGCTG
GCAGATCTTTCGCCACGTGATCCTGCCGGCCATCATGCCCGCGCTGCTCACCGGCTTTGCCATGGCGTTCGCGCGTGCCG
TGGGCGAGTACGGCTCCGTCATCTTCATCGCCGGCAACATGCCCATGGTGTCTGAAATCACGCCGCTGATCATCATCGGC
AAGCTGGAGCAGTACGACTACGCAGGCGCCACTGCCGTGGCGGTGGTGATGCTGGTGATCTCTTTCGTCCTTCTGCTGGT
CATCAACGCCTTGCAGGCCTGGCAGCGCCGCCACGCAGGAGCACCCGCATGA

Upstream 100 bases:

>100_bases
CTGCCCGGGTAGGGTGGCGGTGCCCGCGCATGCAAGCGCGGTGCCTGCGGGCGTGGGCCGCACCGGCTTTTGACTGGCGG
CGAATAACGGATAACTCCAT

Downstream 100 bases:

>100_bases
GCGCCGCGAACACCCGTACCGTGCGGCGCGCGCAGGCCGGAACGACCGAGGCGCGCTGGATCCAGTGGGCGCTGATTGGC
CTTGCCATGGTGTTCCTGCT

Product: sulfate ABC transporter inner membrane subunit cyst

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSGKKSAPRRAKRVLPGFGLTLGYTLFYLSIIVLIPLLALLLKTFTMTWPQFWEAVASPRVLASYRLTFGASFIAACVNL
VFGLLIAWVLVRYKFPGKKIVDALVDLPFALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFNPNGIVIALIFIGLPFV
VRTVQPVLEDSEKELEEAATSLGATRWQIFRHVILPAIMPALLTGFAMAFARAVGEYGSVIFIAGNMPMVSEITPLIIIG
KLEQYDYAGATAVAVVMLVISFVLLLVINALQAWQRRHAGAPA

Sequences:

>Translated_283_residues
MSGKKSAPRRAKRVLPGFGLTLGYTLFYLSIIVLIPLLALLLKTFTMTWPQFWEAVASPRVLASYRLTFGASFIAACVNL
VFGLLIAWVLVRYKFPGKKIVDALVDLPFALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFNPNGIVIALIFIGLPFV
VRTVQPVLEDSEKELEEAATSLGATRWQIFRHVILPAIMPALLTGFAMAFARAVGEYGSVIFIAGNMPMVSEITPLIIIG
KLEQYDYAGATAVAVVMLVISFVLLLVINALQAWQRRHAGAPA
>Mature_282_residues
SGKKSAPRRAKRVLPGFGLTLGYTLFYLSIIVLIPLLALLLKTFTMTWPQFWEAVASPRVLASYRLTFGASFIAACVNLV
FGLLIAWVLVRYKFPGKKIVDALVDLPFALPTAVAGISLTALLAGNGWVGQYLEPLGIQLAFNPNGIVIALIFIGLPFVV
RTVQPVLEDSEKELEEAATSLGATRWQIFRHVILPAIMPALLTGFAMAFARAVGEYGSVIFIAGNMPMVSEITPLIIIGK
LEQYDYAGATAVAVVMLVISFVLLLVINALQAWQRRHAGAPA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=271, Percent_Identity=49.8154981549816, Blast_Score=254, Evalue=4e-69,
Organism=Escherichia coli, GI87082099, Length=251, Percent_Identity=33.8645418326693, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786980, Length=216, Percent_Identity=32.8703703703704, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786254, Length=209, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=29.0322580645161, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787368, Length=222, Percent_Identity=32.4324324324324, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30643; Mature: 30512

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKKSAPRRAKRVLPGFGLTLGYTLFYLSIIVLIPLLALLLKTFTMTWPQFWEAVASPR
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCH
VLASYRLTFGASFIAACVNLVFGLLIAWVLVRYKFPGKKIVDALVDLPFALPTAVAGISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHH
TALLAGNGWVGQYLEPLGIQLAFNPNGIVIALIFIGLPFVVRTVQPVLEDSEKELEEAAT
HHHHCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SLGATRWQIFRHVILPAIMPALLTGFAMAFARAVGEYGSVIFIAGNMPMVSEITPLIIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCEEEEE
KLEQYDYAGATAVAVVMLVISFVLLLVINALQAWQRRHAGAPA
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGKKSAPRRAKRVLPGFGLTLGYTLFYLSIIVLIPLLALLLKTFTMTWPQFWEAVASPR
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCH
VLASYRLTFGASFIAACVNLVFGLLIAWVLVRYKFPGKKIVDALVDLPFALPTAVAGISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHH
TALLAGNGWVGQYLEPLGIQLAFNPNGIVIALIFIGLPFVVRTVQPVLEDSEKELEEAAT
HHHHCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SLGATRWQIFRHVILPAIMPALLTGFAMAFARAVGEYGSVIFIAGNMPMVSEITPLIIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCEEEEE
KLEQYDYAGATAVAVVMLVISFVLLLVINALQAWQRRHAGAPA
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]