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The map label for this gene is rhtB [H]

Identifier: 222110105

GI number: 222110105

Start: 914838

End: 915467

Strand: Direct

Name: rhtB [H]

Synonym: Dtpsy_0891

Alternate gene names: 222110105

Gene position: 914838-915467 (Clockwise)

Preceding gene: 222110104

Following gene: 222110109

Centisome position: 24.1

GC content: 67.3

Gene sequence:

>630_bases
GTGGAAGCCCTGCTGTTTGCCCCCGCCGCCGTTGCCATTGCGCTCACGCCCGGCCCCAACAATTTCTGCGGGCTCAACAA
CGGCATCCGCGCCGGTGTCGCGGCCGCGCTGATCGCGACCGTGGGGCGGGTGGCCGCATTCGCCATTTTCCTCAGTGTCT
CGGCCGTGGGATTGGGGGCCATGCTGCTGGCTTCGGAGGCCGCATTCACGGCCGTCAAATGGGCCGGCGCCGTTTATCTG
TTCTGGATTGGCTGGCGCGCATGGCGCAGCCGGGAGTTCGGTGGCGCGGCGCTGGTCGAAGGAGATGAGGCACCCGCACC
CGCGCGCGCCTCGGTGCGCGCCCTGATGGCACAGGAGTTCCTGCTGGGCATCACCAACCCCAAGGCCATCATCCTCTTTG
CGGCCATCTTCCCGCAGTTCATCGATCCGGCACAGCCCGCGGCGCGGCAGTTCTTGGTGCTGGGCAGCATCTACCTGATG
GCGGAATTCGTGGCCACAGCGGTGTATGCCACGTGCGGGCGGCAGATCCGCCGCGTGATTCGCACGCAGCGCGGCGTGGT
GCGGCTGAACCGTGCCACAGGTGGCTTCTTCATGGGAGCAGGCGGCTTGTTGCTGACGGCCAAGCGCTAA

Upstream 100 bases:

>100_bases
CATTACGATGCGTTGGGCGATCTGCCCTGGCGCCACCGGGTGGTACTGCCGGAATAAACTGGCGCACCTTTTTCCGCGCC
TGCTGGCGCAAGGAGTTGTC

Downstream 100 bases:

>100_bases
AAAAAAGCCGGCCCAGGGGCCGGCTTGCGCGGTGTGCCGCAGGCGCGGGGCCTGCGCAGGAGTATCAGACCACGCCTTGC
GCCAGCATGGCGTCGGCCAC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MEALLFAPAAVAIALTPGPNNFCGLNNGIRAGVAAALIATVGRVAAFAIFLSVSAVGLGAMLLASEAAFTAVKWAGAVYL
FWIGWRAWRSREFGGAALVEGDEAPAPARASVRALMAQEFLLGITNPKAIILFAAIFPQFIDPAQPAARQFLVLGSIYLM
AEFVATAVYATCGRQIRRVIRTQRGVVRLNRATGGFFMGAGGLLLTAKR

Sequences:

>Translated_209_residues
MEALLFAPAAVAIALTPGPNNFCGLNNGIRAGVAAALIATVGRVAAFAIFLSVSAVGLGAMLLASEAAFTAVKWAGAVYL
FWIGWRAWRSREFGGAALVEGDEAPAPARASVRALMAQEFLLGITNPKAIILFAAIFPQFIDPAQPAARQFLVLGSIYLM
AEFVATAVYATCGRQIRRVIRTQRGVVRLNRATGGFFMGAGGLLLTAKR
>Mature_209_residues
MEALLFAPAAVAIALTPGPNNFCGLNNGIRAGVAAALIATVGRVAAFAIFLSVSAVGLGAMLLASEAAFTAVKWAGAVYL
FWIGWRAWRSREFGGAALVEGDEAPAPARASVRALMAQEFLLGITNPKAIILFAAIFPQFIDPAQPAARQFLVLGSIYLM
AEFVATAVYATCGRQIRRVIRTQRGVVRLNRATGGFFMGAGGLLLTAKR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=200, Percent_Identity=32.5, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1788099, Length=192, Percent_Identity=27.0833333333333, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21972; Mature: 21972

Theoretical pI: Translated: 11.54; Mature: 11.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALLFAPAAVAIALTPGPNNFCGLNNGIRAGVAAALIATVGRVAAFAIFLSVSAVGLGA
CCCEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLASEAAFTAVKWAGAVYLFWIGWRAWRSREFGGAALVEGDEAPAPARASVRALMAQEF
HHHHHHHHHHHHHHHHHEEEEEHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
LLGITNPKAIILFAAIFPQFIDPAQPAARQFLVLGSIYLMAEFVATAVYATCGRQIRRVI
HHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTQRGVVRLNRATGGFFMGAGGLLLTAKR
HHHCCEEEEECCCCCEEEECCCEEEEECC
>Mature Secondary Structure
MEALLFAPAAVAIALTPGPNNFCGLNNGIRAGVAAALIATVGRVAAFAIFLSVSAVGLGA
CCCEEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLASEAAFTAVKWAGAVYLFWIGWRAWRSREFGGAALVEGDEAPAPARASVRALMAQEF
HHHHHHHHHHHHHHHHHEEEEEHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
LLGITNPKAIILFAAIFPQFIDPAQPAARQFLVLGSIYLMAEFVATAVYATCGRQIRRVI
HHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTQRGVVRLNRATGGFFMGAGGLLLTAKR
HHHCCEEEEECCCCCEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]