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The map label for this gene is phaA [H]

Identifier: 222109363

GI number: 222109363

Start: 159702

End: 160910

Strand: Direct

Name: phaA [H]

Synonym: Dtpsy_0142

Alternate gene names: 222109363

Gene position: 159702-160910 (Clockwise)

Preceding gene: 222109362

Following gene: 222109364

Centisome position: 4.21

GC content: 71.22

Gene sequence:

>1209_bases
ATGCGCAGAGCCGCCATCGTCACCCCCGTCCGCACCCCCGTCGGCACCTTTGGCGGCAGCCTGCGGCCCGTGCCCGTCGA
GGAGCTGGCCGCCACCACGGTGCGCGCCGTGGTCGAGAGAAGCGGCATCGACCCCGCGCGCATCGACGACGTGGTCATGG
CCCAGTCGTACGCCAACAGCGAGGTGCCCTGCGTGGGCCGCTGGGCCGCGCTGCAGGCGGGCCTGCCGGTGTCGGTGCCG
GGCATGCAGTTGGACCGGCGCTGCGGCGGCGGCCTGCAGGCCATCGTCACCGCGTCGATGATGGTGCAGAGCGGCGCCGC
CGACGTGGTGATCGCCGGCGGCGTGGAGAGCATGAGCAACATCGAGTACTACAGCACCGACATGCGCTGGGGCGCGCGCT
CGGGCAACGTGCGCTTCTACGACCGCCTGGACCGCGGGCGCGAGCGCTCGCAGCCGGTGGAGCGCTTCGGCAAGATCTCC
GGAATGATCGAGACGGCGGAGAACCTGGCGCGCGACTATGGCATTACGCGCGAGGAGGCCGATGCCTTCGCGCTGCGCAG
CCACCAGCGTGCGGCCGCCGCCTGGGAGGCGGGCCGCTTTGCCGACGAAATCGTTCCCGTGCAGGTGCCGCAGCGCAAGG
GCGAGCCGGTCACGTTTGCCCGCGATGAGGGCTTTCGCCCCGACGTCACGCCCGAGAGCCTGGCCAAGCTGCGCGTGCTG
ATGCCGAACGGCACCGTGACCGCCGGCAATGCCAGCCAGCAAAACGATGCCTCGGCCGCCTGCCTGATCGTGGCCGAGGA
CAAGTTGGCCGAGCTGGGCCTCACGCCCATGGCCACGCTGGTGGGCTGGGCGGCCGCGGGCTGCGAGCCCTCGCACATGG
GCATCGGCCCGGTGCCGGCCGTGAAGAAGCTGCTCGCGCGGCTGAACCTGCGCATCGACGACATGGATCTGGTGGAGCTC
AACGAGGCCTTCGCCTGCCAGGTGCTGGCCGTGCTCAAGGGCTGGGAATGGCGGGACCAGGATGCCATCGCGCACAAGCT
CAACGTCAACGGCTCGGGCATCTCGCTGGGCCACCCCATCGGCGCCACGGGCGTGCGCATCCTGGCGACGCTGCTGCACG
AGCTGCAGCGTCGCAAGGGCCGCTACGGCCTGGAGACCATGTGCATCGGCGGCGGCCAGGGCATCGCCGCGGTGTTCGAG
CGCGTCTGA

Upstream 100 bases:

>100_bases
GCTGCGCCGCTGTGGCGCGGTATTTCAAATTTGATAGCTATTAGCGCATGTCAGATAAGCGCTAGCGGCCATTTTCATTC
ATATTTCAAGGAGCAACACC

Downstream 100 bases:

>100_bases
CGCGCGGCGCTTGCACTGAGCGGTGGATCTGCGCCGCCGCAGATCGCCGTGTGTGACTGCGTGACCTGACGCCTTGCACG
GCTGCGGTGCAAGGCGTGCG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MRRAAIVTPVRTPVGTFGGSLRPVPVEELAATTVRAVVERSGIDPARIDDVVMAQSYANSEVPCVGRWAALQAGLPVSVP
GMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSNIEYYSTDMRWGARSGNVRFYDRLDRGRERSQPVERFGKIS
GMIETAENLARDYGITREEADAFALRSHQRAAAAWEAGRFADEIVPVQVPQRKGEPVTFARDEGFRPDVTPESLAKLRVL
MPNGTVTAGNASQQNDASAACLIVAEDKLAELGLTPMATLVGWAAAGCEPSHMGIGPVPAVKKLLARLNLRIDDMDLVEL
NEAFACQVLAVLKGWEWRDQDAIAHKLNVNGSGISLGHPIGATGVRILATLLHELQRRKGRYGLETMCIGGGQGIAAVFE
RV

Sequences:

>Translated_402_residues
MRRAAIVTPVRTPVGTFGGSLRPVPVEELAATTVRAVVERSGIDPARIDDVVMAQSYANSEVPCVGRWAALQAGLPVSVP
GMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSNIEYYSTDMRWGARSGNVRFYDRLDRGRERSQPVERFGKIS
GMIETAENLARDYGITREEADAFALRSHQRAAAAWEAGRFADEIVPVQVPQRKGEPVTFARDEGFRPDVTPESLAKLRVL
MPNGTVTAGNASQQNDASAACLIVAEDKLAELGLTPMATLVGWAAAGCEPSHMGIGPVPAVKKLLARLNLRIDDMDLVEL
NEAFACQVLAVLKGWEWRDQDAIAHKLNVNGSGISLGHPIGATGVRILATLLHELQRRKGRYGLETMCIGGGQGIAAVFE
RV
>Mature_402_residues
MRRAAIVTPVRTPVGTFGGSLRPVPVEELAATTVRAVVERSGIDPARIDDVVMAQSYANSEVPCVGRWAALQAGLPVSVP
GMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSNIEYYSTDMRWGARSGNVRFYDRLDRGRERSQPVERFGKIS
GMIETAENLARDYGITREEADAFALRSHQRAAAAWEAGRFADEIVPVQVPQRKGEPVTFARDEGFRPDVTPESLAKLRVL
MPNGTVTAGNASQQNDASAACLIVAEDKLAELGLTPMATLVGWAAAGCEPSHMGIGPVPAVKKLLARLNLRIDDMDLVEL
NEAFACQVLAVLKGWEWRDQDAIAHKLNVNGSGISLGHPIGATGVRILATLLHELQRRKGRYGLETMCIGGGQGIAAVFE
RV

Specific function: Involved in the production of polyhydroxyalkonic acids (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3- hydroxyvaleric acid (3HV) [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=403, Percent_Identity=41.1910669975186, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI4557237, Length=405, Percent_Identity=37.037037037037, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=36.75, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI4501853, Length=391, Percent_Identity=36.0613810741688, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.3817330210773, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI194353979, Length=383, Percent_Identity=26.6318537859008, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=42.5373134328358, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1787663, Length=420, Percent_Identity=43.8095238095238, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=43.5643564356436, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI48994986, Length=414, Percent_Identity=40.3381642512077, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788683, Length=417, Percent_Identity=31.89448441247, Blast_Score=182, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=40.5, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.0024813895782, Blast_Score=220, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=32.1782178217822, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=30.6976744186047, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=25.8064516129032, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=34.0796019900498, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=34.2431761786601, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24655093, Length=408, Percent_Identity=40.4411764705882, Blast_Score=297, Evalue=9e-81,
Organism=Drosophila melanogaster, GI17648125, Length=401, Percent_Identity=39.4014962593516, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24640423, Length=400, Percent_Identity=35, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17137578, Length=423, Percent_Identity=33.096926713948, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 43082; Mature: 43082

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAAIVTPVRTPVGTFGGSLRPVPVEELAATTVRAVVERSGIDPARIDDVVMAQSYANS
CCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EVPCVGRWAALQAGLPVSVPGMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSN
CCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHC
IEYYSTDMRWGARSGNVRFYDRLDRGRERSQPVERFGKISGMIETAENLARDYGITREEA
CEEEECCCCCCCCCCCEEEHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DAFALRSHQRAAAAWEAGRFADEIVPVQVPQRKGEPVTFARDEGFRPDVTPESLAKLRVL
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCCCCCCHHHHHEEEEE
MPNGTVTAGNASQQNDASAACLIVAEDKLAELGLTPMATLVGWAAAGCEPSHMGIGPVPA
CCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
VKKLLARLNLRIDDMDLVELNEAFACQVLAVLKGWEWRDQDAIAHKLNVNGSGISLGHPI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCEECCCCC
GATGVRILATLLHELQRRKGRYGLETMCIGGGQGIAAVFERV
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MRRAAIVTPVRTPVGTFGGSLRPVPVEELAATTVRAVVERSGIDPARIDDVVMAQSYANS
CCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
EVPCVGRWAALQAGLPVSVPGMQLDRRCGGGLQAIVTASMMVQSGAADVVIAGGVESMSN
CCCCCCHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHC
IEYYSTDMRWGARSGNVRFYDRLDRGRERSQPVERFGKISGMIETAENLARDYGITREEA
CEEEECCCCCCCCCCCEEEHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DAFALRSHQRAAAAWEAGRFADEIVPVQVPQRKGEPVTFARDEGFRPDVTPESLAKLRVL
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCCCCCCHHHHHEEEEE
MPNGTVTAGNASQQNDASAACLIVAEDKLAELGLTPMATLVGWAAAGCEPSHMGIGPVPA
CCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
VKKLLARLNLRIDDMDLVELNEAFACQVLAVLKGWEWRDQDAIAHKLNVNGSGISLGHPI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCEECCCCC
GATGVRILATLLHELQRRKGRYGLETMCIGGGQGIAAVFERV
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782; 10427049 [H]