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The map label for this gene is argT [H]

Identifier: 222102716

GI number: 222102716

Start: 367608

End: 368393

Strand: Direct

Name: argT [H]

Synonym: Avi_7393

Alternate gene names: 222102716

Gene position: 367608-368393 (Clockwise)

Preceding gene: 222102715

Following gene: 222102717

Centisome position: 58.19

GC content: 54.96

Gene sequence:

>786_bases
ATGAAAAAGATCGCAATTGTACTGTCCGTCATCGCCGCAACATTCGGCATGACCAAGCCGTCCTCCGCTGCTGACGATGT
CATTCGTTTCGGCGTGGCCGCTGAGCCTTACGCACCGTTCTCAGTAAAAGATGCCAGCGGCAAATGGCAGGGTTGGGAAA
TCGACCTCATGGACGCCGTTTGCAATCAGATGAAGGCCAAGTGCGAGATCGTTGATGTCGCATGGGACGGCATCATCCCG
GCTCTGCTTGCCAAGAAGTTCGACGTTATCTGGAGTTCCATGTCGATTACCGACGAGCGCAAGAAGGTCATCGACTTCAC
GGACAAGTACTACTACTCTCCCAACGTGCTCGTTGGCGCAAAGGCTGATACACGCAGCTTCGATGTCGCCAAGCCCGAGA
CCTTCAAGGGCATTACCGTTGCAGCGCAAAGCTCCAGCCTGCAGGCCACCTACATGCAGCAGAAATTCAACGGTATGTCT
GACCTCAAGCTCTATCCGACCCTTGATGATGAGATTGCCGACATACAGGCAGGCCGCGTCGACCTCATGGCCGCAGCTGG
CATCCAGATCCAGGACTTCCTGAGCAAGCCTGAGGGGCAGGCTTACGAAATCAAAGTGACATTGCCTCACGAAACGATGT
TCGGTTATGGCGACGGCGGCGGCCTACGCAAGGAAGATACCGCACTGAAGGACAAGTTGAATGCCGCTCTCAAGGCGGTG
CGCGCCAGCGGTGAATACGATGCGATCACCAAGCGCTATTTCAACTTCGACATCTATGGCGAATGA

Upstream 100 bases:

>100_bases
CACGGAAAACTATCTGTTTTCCGTCTGCGAAGCTTTGAGCCGACTGGAGACCGGCTCGACCTGTTAAATCTGCCATCAAC
AAAAAAAGGGGAATTAAAAC

Downstream 100 bases:

>100_bases
TGATCGAGTGAAGGGTTGGAGCCGGTCCTTCATGGCCGGCTTTTCTTCAAACTCGACGAATTGATGCGGCATGGATGCAA
ATATGGCCTGGTTCACTTTA

Product: ABC transporter substrate binding protein (amino acid)

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKKIAIVLSVIAATFGMTKPSSAADDVIRFGVAAEPYAPFSVKDASGKWQGWEIDLMDAVCNQMKAKCEIVDVAWDGIIP
ALLAKKFDVIWSSMSITDERKKVIDFTDKYYYSPNVLVGAKADTRSFDVAKPETFKGITVAAQSSSLQATYMQQKFNGMS
DLKLYPTLDDEIADIQAGRVDLMAAAGIQIQDFLSKPEGQAYEIKVTLPHETMFGYGDGGGLRKEDTALKDKLNAALKAV
RASGEYDAITKRYFNFDIYGE

Sequences:

>Translated_261_residues
MKKIAIVLSVIAATFGMTKPSSAADDVIRFGVAAEPYAPFSVKDASGKWQGWEIDLMDAVCNQMKAKCEIVDVAWDGIIP
ALLAKKFDVIWSSMSITDERKKVIDFTDKYYYSPNVLVGAKADTRSFDVAKPETFKGITVAAQSSSLQATYMQQKFNGMS
DLKLYPTLDDEIADIQAGRVDLMAAAGIQIQDFLSKPEGQAYEIKVTLPHETMFGYGDGGGLRKEDTALKDKLNAALKAV
RASGEYDAITKRYFNFDIYGE
>Mature_261_residues
MKKIAIVLSVIAATFGMTKPSSAADDVIRFGVAAEPYAPFSVKDASGKWQGWEIDLMDAVCNQMKAKCEIVDVAWDGIIP
ALLAKKFDVIWSSMSITDERKKVIDFTDKYYYSPNVLVGAKADTRSFDVAKPETFKGITVAAQSSSLQATYMQQKFNGMS
DLKLYPTLDDEIADIQAGRVDLMAAAGIQIQDFLSKPEGQAYEIKVTLPHETMFGYGDGGGLRKEDTALKDKLNAALKAV
RASGEYDAITKRYFNFDIYGE

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=266, Percent_Identity=34.5864661654135, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788648, Length=238, Percent_Identity=36.9747899159664, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787085, Length=255, Percent_Identity=31.3725490196078, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787088, Length=255, Percent_Identity=30.1960784313725, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=31.1023622047244, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=23.4234234234234, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28710; Mature: 28710

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIVLSVIAATFGMTKPSSAADDVIRFGVAAEPYAPFSVKDASGKWQGWEIDLMDAV
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEHHHHH
CNQMKAKCEIVDVAWDGIIPALLAKKFDVIWSSMSITDERKKVIDFTDKYYYSPNVLVGA
HHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCEEEEC
KADTRSFDVAKPETFKGITVAAQSSSLQATYMQQKFNGMSDLKLYPTLDDEIADIQAGRV
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCE
DLMAAAGIQIQDFLSKPEGQAYEIKVTLPHETMFGYGDGGGLRKEDTALKDKLNAALKAV
EEEEECCCCHHHHHCCCCCCEEEEEEEECCHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
RASGEYDAITKRYFNFDIYGE
HCCCCHHHHHHHEEEEEEECC
>Mature Secondary Structure
MKKIAIVLSVIAATFGMTKPSSAADDVIRFGVAAEPYAPFSVKDASGKWQGWEIDLMDAV
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEHHHHH
CNQMKAKCEIVDVAWDGIIPALLAKKFDVIWSSMSITDERKKVIDFTDKYYYSPNVLVGA
HHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCEEEEC
KADTRSFDVAKPETFKGITVAAQSSSLQATYMQQKFNGMSDLKLYPTLDDEIADIQAGRV
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCE
DLMAAAGIQIQDFLSKPEGQAYEIKVTLPHETMFGYGDGGGLRKEDTALKDKLNAALKAV
EEEEECCCCHHHHHCCCCCCEEEEEEEECCHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
RASGEYDAITKRYFNFDIYGE
HCCCCHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]