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The map label for this gene is gatA [H]

Identifier: 222102715

GI number: 222102715

Start: 366219

End: 367574

Strand: Direct

Name: gatA [H]

Synonym: Avi_7392

Alternate gene names: 222102715

Gene position: 366219-367574 (Clockwise)

Preceding gene: 222102714

Following gene: 222102716

Centisome position: 57.97

GC content: 57.67

Gene sequence:

>1356_bases
GTGAACCTGGTGGCGAAACCCTTTCCTCAGACGATTACCCAAGCTCAAGGCCAGTTTCTGGAAGGATTGTCGCCCCTCTC
GGTCGTCAAGACGTGCCTTGAGCGTATCCAGGAACAGAACGATAAACTTGGGGCGATGATCTTCATAGCGCGTGCGGAGG
CGCTTGAAGAAGCGCAAAAAGCGGAAGCGGCAATTCGAGCTGGAAAACGAATTGGCCCGCTGCACGGTATTCCCGTTGCG
ATCAAAGACATGATCGATGTTCGTGGCTGGCAAACGACCGGTGGATCTCACCTGCTCCATGGGGACCATGCAGTGAACGA
CGCCCGTTGTGTGGCAAACCTGAGATCGGCTGGCGCGATCATTATTGGCAAAAGCAATCTGCACGAACTCACGGCAGGCA
ACCACGATAATCCCTGGTACGGAAAGGTTGTCAATCCGCTGGACGCCGAAAGAGGCACCGGCGGAACGAGCAGCGGTTCG
GCGGCAGCGGTAGCAGCGGGCTTTTGTATCGCCGCCATCGGAACCGACACCGGTGGATCGAACCGCTCAACCGCCGCAGC
GACCGGTCTCGTGGGGTTCAAGCCAACCAATGGTGTCATTGACCCGACAGGCACGATGCCCACCGCGCCGACCTTCGATA
CAATCGGTCCAATCGCTAGAAACGTGGCGGATGCCCGTCTGCTTCATTATGCCATGGCAGGAATCGATACGCCGGAGAAG
AGACGTGTGTCGGTTGCGGGAATGCGAATTGGTCTGTGCCCCGATTTATATGCAGCAGCGGTTGACCCCGCAATTGCAAC
CGCACATGACGCGTTTCTGGCCTTAGCACGAAGGGCGGGCGCGCAAATCCAGGTCCTCCCATTCCGGTTTGCCCGCGAGG
TTCGCGAAGCAGGGCTGACAATCCTGGTGTATGAGTTCACGGCTTTCTATGGCCCGCTTGTCGAAGCGCATCCCGGGCGG
GTTGGGGCAGCGGTTCTCGCGTTTCTATCGAATGGAGCCTTGATTTCAGAAGCCGCCTATCGATCAGCGCTCGCGTACCA
AGCGCAAATCCAAGGTGAATTTCACAGACTGTTGTCAGAAGTCGATGTTCTGCTGACGCCAGTTGTGCCGGGACTTGCAC
CACGGCTGTCGGACGAGCAGACGGCAGTGGGAAACGACTTCATGCCTTATGGCCTGGCCGGAGGTCACTTTCGCCGCTGG
GCAAACTTTTTCGGTGTACCAGCGCTTGCAATGCCGGTGCCCATGGCGGGTGCGCTTCCAGCATCCATCCAGATCACAAC
GCCTAGAAACACGGAAAACTATCTGTTTTCCGTCTGCGAAGCTTTGAGCCGACTGGAGACCGGCTCGACCTGTTAA

Upstream 100 bases:

>100_bases
CGTTATAACTATTGGTAATCAACCCACGGCCTGCAGGAATTAGGAAGTCTGAAATCGACCTGTTAGCGTCATTTTGACTA
AAGCCAAGACCTGAGAGTGA

Downstream 100 bases:

>100_bases
ATCTGCCATCAACAAAAAAAGGGGAATTAAAACATGAAAAAGATCGCAATTGTACTGTCCGTCATCGCCGCAACATTCGG
CATGACCAAGCCGTCCTCCG

Product: glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNLVAKPFPQTITQAQGQFLEGLSPLSVVKTCLERIQEQNDKLGAMIFIARAEALEEAQKAEAAIRAGKRIGPLHGIPVA
IKDMIDVRGWQTTGGSHLLHGDHAVNDARCVANLRSAGAIIIGKSNLHELTAGNHDNPWYGKVVNPLDAERGTGGTSSGS
AAAVAAGFCIAAIGTDTGGSNRSTAAATGLVGFKPTNGVIDPTGTMPTAPTFDTIGPIARNVADARLLHYAMAGIDTPEK
RRVSVAGMRIGLCPDLYAAAVDPAIATAHDAFLALARRAGAQIQVLPFRFAREVREAGLTILVYEFTAFYGPLVEAHPGR
VGAAVLAFLSNGALISEAAYRSALAYQAQIQGEFHRLLSEVDVLLTPVVPGLAPRLSDEQTAVGNDFMPYGLAGGHFRRW
ANFFGVPALAMPVPMAGALPASIQITTPRNTENYLFSVCEALSRLETGSTC

Sequences:

>Translated_451_residues
MNLVAKPFPQTITQAQGQFLEGLSPLSVVKTCLERIQEQNDKLGAMIFIARAEALEEAQKAEAAIRAGKRIGPLHGIPVA
IKDMIDVRGWQTTGGSHLLHGDHAVNDARCVANLRSAGAIIIGKSNLHELTAGNHDNPWYGKVVNPLDAERGTGGTSSGS
AAAVAAGFCIAAIGTDTGGSNRSTAAATGLVGFKPTNGVIDPTGTMPTAPTFDTIGPIARNVADARLLHYAMAGIDTPEK
RRVSVAGMRIGLCPDLYAAAVDPAIATAHDAFLALARRAGAQIQVLPFRFAREVREAGLTILVYEFTAFYGPLVEAHPGR
VGAAVLAFLSNGALISEAAYRSALAYQAQIQGEFHRLLSEVDVLLTPVVPGLAPRLSDEQTAVGNDFMPYGLAGGHFRRW
ANFFGVPALAMPVPMAGALPASIQITTPRNTENYLFSVCEALSRLETGSTC
>Mature_451_residues
MNLVAKPFPQTITQAQGQFLEGLSPLSVVKTCLERIQEQNDKLGAMIFIARAEALEEAQKAEAAIRAGKRIGPLHGIPVA
IKDMIDVRGWQTTGGSHLLHGDHAVNDARCVANLRSAGAIIIGKSNLHELTAGNHDNPWYGKVVNPLDAERGTGGTSSGS
AAAVAAGFCIAAIGTDTGGSNRSTAAATGLVGFKPTNGVIDPTGTMPTAPTFDTIGPIARNVADARLLHYAMAGIDTPEK
RRVSVAGMRIGLCPDLYAAAVDPAIATAHDAFLALARRAGAQIQVLPFRFAREVREAGLTILVYEFTAFYGPLVEAHPGR
VGAAVLAFLSNGALISEAAYRSALAYQAQIQGEFHRLLSEVDVLLTPVVPGLAPRLSDEQTAVGNDFMPYGLAGGHFRRW
ANFFGVPALAMPVPMAGALPASIQITTPRNTENYLFSVCEALSRLETGSTC

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=242, Percent_Identity=35.9504132231405, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI195972892, Length=473, Percent_Identity=24.1014799154334, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=447, Percent_Identity=28.1879194630872, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=30.2521008403361, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=229, Percent_Identity=30.1310043668122, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=228, Percent_Identity=30.2631578947368, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=229, Percent_Identity=31.0043668122271, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=387, Percent_Identity=30.2325581395349, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=176, Percent_Identity=34.6590909090909, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=27.4193548387097, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24652985, Length=468, Percent_Identity=27.3504273504274, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19922090, Length=468, Percent_Identity=27.3504273504274, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24652981, Length=468, Percent_Identity=27.3504273504274, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24652983, Length=468, Percent_Identity=27.3504273504274, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648435, Length=239, Percent_Identity=33.0543933054393, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648437, Length=239, Percent_Identity=33.0543933054393, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550774, Length=239, Percent_Identity=33.0543933054393, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356731, Length=209, Percent_Identity=33.9712918660287, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648441, Length=196, Percent_Identity=33.1632653061224, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648439, Length=196, Percent_Identity=33.1632653061224, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24644968, Length=330, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161078093, Length=474, Percent_Identity=25.9493670886076, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47427; Mature: 47427

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVAKPFPQTITQAQGQFLEGLSPLSVVKTCLERIQEQNDKLGAMIFIARAEALEEAQK
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHH
AEAAIRAGKRIGPLHGIPVAIKDMIDVRGWQTTGGSHLLHGDHAVNDARCVANLRSAGAI
HHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCEE
IIGKSNLHELTAGNHDNPWYGKVVNPLDAERGTGGTSSGSAAAVAAGFCIAAIGTDTGGS
EEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCCC
NRSTAAATGLVGFKPTNGVIDPTGTMPTAPTFDTIGPIARNVADARLLHYAMAGIDTPEK
CCCCHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RRVSVAGMRIGLCPDLYAAAVDPAIATAHDAFLALARRAGAQIQVLPFRFAREVREAGLT
HEEEECCEEECCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCE
ILVYEFTAFYGPLVEAHPGRVGAAVLAFLSNGALISEAAYRSALAYQAQIQGEFHRLLSE
EEEEEHHHHHCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDVLLTPVVPGLAPRLSDEQTAVGNDFMPYGLAGGHFRRWANFFGVPALAMPVPMAGALP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCC
ASIQITTPRNTENYLFSVCEALSRLETGSTC
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLVAKPFPQTITQAQGQFLEGLSPLSVVKTCLERIQEQNDKLGAMIFIARAEALEEAQK
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHH
AEAAIRAGKRIGPLHGIPVAIKDMIDVRGWQTTGGSHLLHGDHAVNDARCVANLRSAGAI
HHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCEE
IIGKSNLHELTAGNHDNPWYGKVVNPLDAERGTGGTSSGSAAAVAAGFCIAAIGTDTGGS
EEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCCCC
NRSTAAATGLVGFKPTNGVIDPTGTMPTAPTFDTIGPIARNVADARLLHYAMAGIDTPEK
CCCCHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RRVSVAGMRIGLCPDLYAAAVDPAIATAHDAFLALARRAGAQIQVLPFRFAREVREAGLT
HEEEECCEEECCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCE
ILVYEFTAFYGPLVEAHPGRVGAAVLAFLSNGALISEAAYRSALAYQAQIQGEFHRLLSE
EEEEEHHHHHCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDVLLTPVVPGLAPRLSDEQTAVGNDFMPYGLAGGHFRRWANFFGVPALAMPVPMAGALP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCC
ASIQITTPRNTENYLFSVCEALSRLETGSTC
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA