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The map label for this gene is nepI [H]

Identifier: 222102707

GI number: 222102707

Start: 356732

End: 357940

Strand: Reverse

Name: nepI [H]

Synonym: Avi_7381

Alternate gene names: 222102707

Gene position: 357940-356732 (Counterclockwise)

Preceding gene: 222102708

Following gene: 222102704

Centisome position: 56.66

GC content: 60.63

Gene sequence:

>1209_bases
ATGACGGACAGAACATCACAATTTAACAGCGCAGCTATAGACCTCGATACCGCTGAGCCGGTTGCGTGGAGCCCTGCCAC
CTGGTTTGCCGTCCTTTCGATGGCGGCCACCAGTTTTGCGCTGGTGTCGGCTGAGTTCCTGCCAGCAGGCTTGCTGACGC
CTATGGCCCGTGATCTTGGGATCAGCGAGGGAACAGCCGGACAGGTCGTGACTGCCACGGCTTTCGTGGGCGCCATCACG
GCCCTGTTGAGCAATGTCCTGATCGGCAGATTAAACCGCAAGACAGTACTGGTCGGCCTCAGTGGCTTGGCAATCGGCTC
CAATATTCTTGCAGCGGTGGCGACGGACTTTTGGCTATTGATGCTCGGTCGGGCAGGCCTCGGCATTGCCCTCAGCGGCT
TCTGGGCGCTTTCGGTTGCCGTCGTCGCCAGGTTGGTTGGGGCCAATGCAACAGGACGGGGCATGGCGATCGTCACGCTC
GGCGTCTCACTCGCAACGATAGCCGCGCCATCGATAGGCGCTTTGATCAGCGATTGGCTGGGCTGGCGCAGCGCCATGTC
GATGACCGCGGGACTTGCTGCAATTGCCATGTTGCTACAGGTAATCTGCTTGCCGACGCTGCCTGCAAGCACAAGCAACA
GTCTCTCTGATGTTTTCCGGCTGACGCGGCGGCGTGGCGTTCAACTTGGAATGTTGGCCATCCTTTTGTTGATGACGGGA
CATTTTGCCGGCTCGGTTTATGTCCGTCCTTTCCTTGAACAGGTGACCCTGCTTGAAACCGGACCGATTGCCCTGACACT
TCTCGGGTTTGGCGTTGCCTCGGTGATCGGTAACGTTGCGGGTGGCCGGATGGCGGACGCCAGTATTCATATGGCACTCG
CTGTCACCGCCGCGCTGATGGCATCTGCGGCGCTCGCCTTGGTGCTTTGGGGCAGTTATGTCGGCGTTGCCTTCAGCCTG
GTAACGCTTTGGGGCTTTGCCTTCGGCATGGCACCCGTGGTGCTGCCGACCAACCTATCGCGCAGCGCACCTGACGCCCT
GGAGGCAGCGGGCAGCCTGATGGTCGTGTCTTTCCAGGTCGCGATCACGATTGGCGCGGTTGTCGGCGGCTATGTCGTCG
ATCATTATGGCGCTGCGGGACCCTTGACCCTGACAGCCGCCCTGGCCGCATTGACTGTTGTCTTGGCGCTGACGCAACCG
CGCAGCTGA

Upstream 100 bases:

>100_bases
CACAGTTTCCGGACGAACGGACATGGTTTTCGGATTTTAAGCTACATATAATCCCAAATCTTTCGTCTATGAAGTGCTTC
TGGATTCAGGAGATACGTCA

Downstream 100 bases:

>100_bases
TCTTCCCTTTCGGGTCAGCCGCTGTTTCAGCTTTGAGCCTCGACGTGAACATGAACAACCAGTTGATGCTCCACGACGAG
ATCAGCGAAGCTGCTGCCCC

Product: permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTDRTSQFNSAAIDLDTAEPVAWSPATWFAVLSMAATSFALVSAEFLPAGLLTPMARDLGISEGTAGQVVTATAFVGAIT
ALLSNVLIGRLNRKTVLVGLSGLAIGSNILAAVATDFWLLMLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTL
GVSLATIAAPSIGALISDWLGWRSAMSMTAGLAAIAMLLQVICLPTLPASTSNSLSDVFRLTRRRGVQLGMLAILLLMTG
HFAGSVYVRPFLEQVTLLETGPIALTLLGFGVASVIGNVAGGRMADASIHMALAVTAALMASAALALVLWGSYVGVAFSL
VTLWGFAFGMAPVVLPTNLSRSAPDALEAAGSLMVVSFQVAITIGAVVGGYVVDHYGAAGPLTLTAALAALTVVLALTQP
RS

Sequences:

>Translated_402_residues
MTDRTSQFNSAAIDLDTAEPVAWSPATWFAVLSMAATSFALVSAEFLPAGLLTPMARDLGISEGTAGQVVTATAFVGAIT
ALLSNVLIGRLNRKTVLVGLSGLAIGSNILAAVATDFWLLMLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTL
GVSLATIAAPSIGALISDWLGWRSAMSMTAGLAAIAMLLQVICLPTLPASTSNSLSDVFRLTRRRGVQLGMLAILLLMTG
HFAGSVYVRPFLEQVTLLETGPIALTLLGFGVASVIGNVAGGRMADASIHMALAVTAALMASAALALVLWGSYVGVAFSL
VTLWGFAFGMAPVVLPTNLSRSAPDALEAAGSLMVVSFQVAITIGAVVGGYVVDHYGAAGPLTLTAALAALTVVLALTQP
RS
>Mature_401_residues
TDRTSQFNSAAIDLDTAEPVAWSPATWFAVLSMAATSFALVSAEFLPAGLLTPMARDLGISEGTAGQVVTATAFVGAITA
LLSNVLIGRLNRKTVLVGLSGLAIGSNILAAVATDFWLLMLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTLG
VSLATIAAPSIGALISDWLGWRSAMSMTAGLAAIAMLLQVICLPTLPASTSNSLSDVFRLTRRRGVQLGMLAILLLMTGH
FAGSVYVRPFLEQVTLLETGPIALTLLGFGVASVIGNVAGGRMADASIHMALAVTAALMASAALALVLWGSYVGVAFSLV
TLWGFAFGMAPVVLPTNLSRSAPDALEAAGSLMVVSFQVAITIGAVVGGYVVDHYGAAGPLTLTAALAALTVVLALTQPR
S

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=369, Percent_Identity=38.4823848238482, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1787808, Length=353, Percent_Identity=25.4957507082153, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787947, Length=300, Percent_Identity=27.3333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786595, Length=259, Percent_Identity=24.7104247104247, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40869; Mature: 40737

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRTSQFNSAAIDLDTAEPVAWSPATWFAVLSMAATSFALVSAEFLPAGLLTPMARDLG
CCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ISEGTAGQVVTATAFVGAITALLSNVLIGRLNRKTVLVGLSGLAIGSNILAAVATDFWLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
MLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTLGVSLATIAAPSIGALISDWL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHCCHHHHHHHHHH
GWRSAMSMTAGLAAIAMLLQVICLPTLPASTSNSLSDVFRLTRRRGVQLGMLAILLLMTG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC
HFAGSVYVRPFLEQVTLLETGPIALTLLGFGVASVIGNVAGGRMADASIHMALAVTAALM
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASAALALVLWGSYVGVAFSLVTLWGFAFGMAPVVLPTNLSRSAPDALEAAGSLMVVSFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCEEEEEHHH
AITIGAVVGGYVVDHYGAAGPLTLTAALAALTVVLALTQPRS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDRTSQFNSAAIDLDTAEPVAWSPATWFAVLSMAATSFALVSAEFLPAGLLTPMARDLG
CCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ISEGTAGQVVTATAFVGAITALLSNVLIGRLNRKTVLVGLSGLAIGSNILAAVATDFWLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
MLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTLGVSLATIAAPSIGALISDWL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHCCHHHHHHHHHH
GWRSAMSMTAGLAAIAMLLQVICLPTLPASTSNSLSDVFRLTRRRGVQLGMLAILLLMTG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC
HFAGSVYVRPFLEQVTLLETGPIALTLLGFGVASVIGNVAGGRMADASIHMALAVTAALM
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASAALALVLWGSYVGVAFSLVTLWGFAFGMAPVVLPTNLSRSAPDALEAAGSLMVVSFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCEEEEEHHH
AITIGAVVGGYVVDHYGAAGPLTLTAALAALTVVLALTQPRS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA