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The map label for this gene is xylF [H]

Identifier: 222102704

GI number: 222102704

Start: 353313

End: 354359

Strand: Reverse

Name: xylF [H]

Synonym: Avi_7378

Alternate gene names: 222102704

Gene position: 354359-353313 (Counterclockwise)

Preceding gene: 222102707

Following gene: 222102703

Centisome position: 56.09

GC content: 55.3

Gene sequence:

>1047_bases
ATGAAATTTCATTCCATGATGATGGTGTCGGTTGCTGCCGCTGCCATGTTTGCAGGCTCTGCCTTGGCGCAGGAGAAGGC
AACGGTTGCGTTTCTGATGCCCGATCAGGCATCGACACGCTATGAAAACCACGATTTTCCGGGCTTCAAGGCAGAAATGG
CCAAGCTGTGCGCCGGATGCACGGTGATTTATCAAAACGCCAATGGCGATACCGCACTTCAGCAACAGCAATTCAACTCA
GTGATTGCGCAAGGCGCAAAGGTGATTGTGCTGGACCCGGTGGATTCGTCTGCTGCCGCAGCACTGGTCGAATTGGCGCA
GTCGCAGGATGTGAAGGTGATTGCCTATGACCGTCCTATTCCCAAGAAAGCAGCGGATTATTACGTCTCCTTCGACAATG
CTGGCATTGGCGAGGCTATTGCCAAGTCTTTGGTGGAGCACCTGAAGGCCAAGGGCGTGCCGCAGGGTTCTGGCATTCTT
CAAATCAACGGTTCGCCCACCGATGCCGCCGCTGGCCTGATCCGCGATGGTGTTCATAAAGGCCTGAAGGATTCTGGCTA
CAAAACACTGGCTGAATTCGACACGCCGGAATGGGCACCACCAAAGGCGCAGGAATGGGCGGCAGGTCAGATCACCCGTT
TTGGCGCAGACATCAAGGGCGTTGTTGCCGCCAATGACGGCACAGGCGGCGGTGCGATTGCGGCCTTCAAGGCGGCTGGT
GTTAATCCGGTGCCACCCGTTACCGGCAATGATGCGACCATTGCAGCACTTCAGCTGATCATTTCCGGCGACCAGTACAA
CACGATTTCCAAGCCATCGGAAATTGTTGCGGCGGCGGCTGCCAAAGTCACCGTTCAGTTTCTGAAGGGCGAAAAGCCGG
AAGCCAAGACCACGCTGTATGACACCCCCTCGCAGCTGTTCATTCCGGCTGTTGTGACGGCAGAAAACATCAAGGCAGAA
ATCTTTGACAAGAAGATTCAGACGCCTGCCGAAGTCTGCACGGGTGAATATGCCACTGGCTGCAAAAAGCTGGGCATCAC
CCAGTAA

Upstream 100 bases:

>100_bases
AGGATTGTTGTCGCATCATCGTGCGTTTGATGTGCCGTGATCACGGTCTTCATCGGCATGCGTCTCTCGATACTGGTTTT
GGGTTCTAGAGGAGGAAGCT

Downstream 100 bases:

>100_bases
TTGTTCTGATCTGTGTTCGGTCCGGCATCGCCGGATCGGACATGCCCCGAACCTGAAAAGAGCGTTTCGCCATGGTGCAA
AATGACCAAGCGGCCCCGAA

Product: ABC transporter substrate binding protein (xylose)

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKFHSMMMVSVAAAAMFAGSALAQEKATVAFLMPDQASTRYENHDFPGFKAEMAKLCAGCTVIYQNANGDTALQQQQFNS
VIAQGAKVIVLDPVDSSAAAALVELAQSQDVKVIAYDRPIPKKAADYYVSFDNAGIGEAIAKSLVEHLKAKGVPQGSGIL
QINGSPTDAAAGLIRDGVHKGLKDSGYKTLAEFDTPEWAPPKAQEWAAGQITRFGADIKGVVAANDGTGGGAIAAFKAAG
VNPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAAAAAKVTVQFLKGEKPEAKTTLYDTPSQLFIPAVVTAENIKAE
IFDKKIQTPAEVCTGEYATGCKKLGITQ

Sequences:

>Translated_348_residues
MKFHSMMMVSVAAAAMFAGSALAQEKATVAFLMPDQASTRYENHDFPGFKAEMAKLCAGCTVIYQNANGDTALQQQQFNS
VIAQGAKVIVLDPVDSSAAAALVELAQSQDVKVIAYDRPIPKKAADYYVSFDNAGIGEAIAKSLVEHLKAKGVPQGSGIL
QINGSPTDAAAGLIRDGVHKGLKDSGYKTLAEFDTPEWAPPKAQEWAAGQITRFGADIKGVVAANDGTGGGAIAAFKAAG
VNPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAAAAAKVTVQFLKGEKPEAKTTLYDTPSQLFIPAVVTAENIKAE
IFDKKIQTPAEVCTGEYATGCKKLGITQ
>Mature_348_residues
MKFHSMMMVSVAAAAMFAGSALAQEKATVAFLMPDQASTRYENHDFPGFKAEMAKLCAGCTVIYQNANGDTALQQQQFNS
VIAQGAKVIVLDPVDSSAAAALVELAQSQDVKVIAYDRPIPKKAADYYVSFDNAGIGEAIAKSLVEHLKAKGVPQGSGIL
QINGSPTDAAAGLIRDGVHKGLKDSGYKTLAEFDTPEWAPPKAQEWAAGQITRFGADIKGVVAANDGTGGGAIAAFKAAG
VNPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAAAAAKVTVQFLKGEKPEAKTTLYDTPSQLFIPAVVTAENIKAE
IFDKKIQTPAEVCTGEYATGCKKLGITQ

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=282, Percent_Identity=36.1702127659575, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1788473, Length=228, Percent_Identity=30.7017543859649, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36338; Mature: 36338

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFHSMMMVSVAAAAMFAGSALAQEKATVAFLMPDQASTRYENHDFPGFKAEMAKLCAGC
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCC
TVIYQNANGDTALQQQQFNSVIAQGAKVIVLDPVDSSAAAALVELAQSQDVKVIAYDRPI
EEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCC
PKKAADYYVSFDNAGIGEAIAKSLVEHLKAKGVPQGSGILQINGSPTDAAAGLIRDGVHK
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
GLKDSGYKTLAEFDTPEWAPPKAQEWAAGQITRFGADIKGVVAANDGTGGGAIAAFKAAG
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCEEEEEECCC
VNPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAAAAAKVTVQFLKGEKPEAKTTLY
CCCCCCCCCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
DTPSQLFIPAVVTAENIKAEIFDKKIQTPAEVCTGEYATGCKKLGITQ
CCCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKFHSMMMVSVAAAAMFAGSALAQEKATVAFLMPDQASTRYENHDFPGFKAEMAKLCAGC
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCC
TVIYQNANGDTALQQQQFNSVIAQGAKVIVLDPVDSSAAAALVELAQSQDVKVIAYDRPI
EEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCC
PKKAADYYVSFDNAGIGEAIAKSLVEHLKAKGVPQGSGILQINGSPTDAAAGLIRDGVHK
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
GLKDSGYKTLAEFDTPEWAPPKAQEWAAGQITRFGADIKGVVAANDGTGGGAIAAFKAAG
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCCCCEEEEEECCC
VNPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAAAAAKVTVQFLKGEKPEAKTTLY
CCCCCCCCCCCHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
DTPSQLFIPAVVTAENIKAEIFDKKIQTPAEVCTGEYATGCKKLGITQ
CCCCCEEEEEEEECCHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]