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The map label for this gene is rpiR [H]

Identifier: 222102695

GI number: 222102695

Start: 343453

End: 344328

Strand: Direct

Name: rpiR [H]

Synonym: Avi_7367

Alternate gene names: 222102695

Gene position: 343453-344328 (Clockwise)

Preceding gene: 222102694

Following gene: 222102696

Centisome position: 54.36

GC content: 57.65

Gene sequence:

>876_bases
ATGAACGCTGATGGCTTTGACATGAAGGCGGTAGGGCCGCGCATTCGCATGATGATGCCAATGCTGACCCCGCTGGAAGC
AAAGGTCGTGGATACGGTTTTTGGCATGCGTGATTTCAGCGAGGAGACCTCGCTGAAGCAAATCGCCGAAAATGCCGGGG
TTTCGGAAGCCATGGTGGTGAAGATCACCAAGAAGCTCGGTTTTTCCGGCTTTCGGGATTTCCGGTCTTCCGTCAGCCAG
TATAACCGCCAGCCGACCACCGAAATGCATCAGGAATTGTCGGTTGACGATACCTCGCTTGAAATCGTTCAGAAAGTCTT
TCGCACCTCGATCCACGCCCTTGAGGAAACACTTGCCATTCTGGACATGGCAGCCTTCGACCAGGCGGCGGACATGATCC
ATAAGGCTCGAAACCGGGATTTCTACGGCGTCGGCGGCTCGGCGCAGATCGCCCGGGATGTCGCCCACAAATTTTTGAGA
ATTGGCGTGCGCGCCAGCGTCTTCGATGATTCACACATGATGCTGATGTCGGCGGCCCTTCTCGCCGATACCGATGTCGC
CATTGGCTTTTCGCATTCGGGCAACACGATTGCCGTGATCGAGGCCCTGCAACTTGCCCGCAAGAACGGTGCCAGCACCA
TTGCCGTGACCAATTACGGTTCCTCCGCGCTCGCCCAGTCCGCCGACGTGGTCTTGTGTTCCACCGCACAAGGCTCGCCT
TTGATGGGGGAAAATGCCGCGGCGCGCATTGCGCAGCTCAATATTCTCGACGCCATTTTCGTCGCCGTCGCCCAGCGCGA
CTATCAGGCCGCCGAGCGCAATCTCGACCGCACCATGTCCGCCGTCACCTCTAAACGTAGAGATCGCCTTCCATGA

Upstream 100 bases:

>100_bases
GTTTGACGCTGATGAATGTCCAGGCCTTCTATCAACTGCTCGCCACCGGCCTTATAATCCTCGTCGCAATGATGATTGAT
CGCGCGACAAGGGGACGGGA

Downstream 100 bases:

>100_bases
CATTCTCGCCTATCGTCACCGTTTTCGGCAGCCTGCATTATGATATCGCGGTCTTCGGTCCAGACCGGCCACGTCAGGGA
GAAACTGTTGCAGGCACGTC

Product: DNA-binding transcriptional repressor RpiR

Products: NA

Alternate protein names: Als operon repressor [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNADGFDMKAVGPRIRMMMPMLTPLEAKVVDTVFGMRDFSEETSLKQIAENAGVSEAMVVKITKKLGFSGFRDFRSSVSQ
YNRQPTTEMHQELSVDDTSLEIVQKVFRTSIHALEETLAILDMAAFDQAADMIHKARNRDFYGVGGSAQIARDVAHKFLR
IGVRASVFDDSHMMLMSAALLADTDVAIGFSHSGNTIAVIEALQLARKNGASTIAVTNYGSSALAQSADVVLCSTAQGSP
LMGENAAARIAQLNILDAIFVAVAQRDYQAAERNLDRTMSAVTSKRRDRLP

Sequences:

>Translated_291_residues
MNADGFDMKAVGPRIRMMMPMLTPLEAKVVDTVFGMRDFSEETSLKQIAENAGVSEAMVVKITKKLGFSGFRDFRSSVSQ
YNRQPTTEMHQELSVDDTSLEIVQKVFRTSIHALEETLAILDMAAFDQAADMIHKARNRDFYGVGGSAQIARDVAHKFLR
IGVRASVFDDSHMMLMSAALLADTDVAIGFSHSGNTIAVIEALQLARKNGASTIAVTNYGSSALAQSADVVLCSTAQGSP
LMGENAAARIAQLNILDAIFVAVAQRDYQAAERNLDRTMSAVTSKRRDRLP
>Mature_291_residues
MNADGFDMKAVGPRIRMMMPMLTPLEAKVVDTVFGMRDFSEETSLKQIAENAGVSEAMVVKITKKLGFSGFRDFRSSVSQ
YNRQPTTEMHQELSVDDTSLEIVQKVFRTSIHALEETLAILDMAAFDQAADMIHKARNRDFYGVGGSAQIARDVAHKFLR
IGVRASVFDDSHMMLMSAALLADTDVAIGFSHSGNTIAVIEALQLARKNGASTIAVTNYGSSALAQSADVVLCSTAQGSP
LMGENAAARIAQLNILDAIFVAVAQRDYQAAERNLDRTMSAVTSKRRDRLP

Specific function: Regulatory protein involved in rpiB gene repression. Also involved in als operon repression [H]

COG id: COG1737

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI87082369, Length=273, Percent_Identity=41.7582417582418, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1788767, Length=254, Percent_Identity=31.8897637795276, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788159, Length=276, Percent_Identity=28.2608695652174, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87082130, Length=269, Percent_Identity=27.1375464684015, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01418 HTH_6; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 31672; Mature: 31672

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51071 HTH_RPIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNADGFDMKAVGPRIRMMMPMLTPLEAKVVDTVFGMRDFSEETSLKQIAENAGVSEAMVV
CCCCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KITKKLGFSGFRDFRSSVSQYNRQPTTEMHQELSVDDTSLEIVQKVFRTSIHALEETLAI
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LDMAAFDQAADMIHKARNRDFYGVGGSAQIARDVAHKFLRIGVRASVFDDSHMMLMSAAL
HHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHH
LADTDVAIGFSHSGNTIAVIEALQLARKNGASTIAVTNYGSSALAQSADVVLCSTAQGSP
HHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCEEEEECCCCCC
LMGENAAARIAQLNILDAIFVAVAQRDYQAAERNLDRTMSAVTSKRRDRLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNADGFDMKAVGPRIRMMMPMLTPLEAKVVDTVFGMRDFSEETSLKQIAENAGVSEAMVV
CCCCCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KITKKLGFSGFRDFRSSVSQYNRQPTTEMHQELSVDDTSLEIVQKVFRTSIHALEETLAI
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LDMAAFDQAADMIHKARNRDFYGVGGSAQIARDVAHKFLRIGVRASVFDDSHMMLMSAAL
HHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHH
LADTDVAIGFSHSGNTIAVIEALQLARKNGASTIAVTNYGSSALAQSADVVLCSTAQGSP
HHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCEEEEECCCCCC
LMGENAAARIAQLNILDAIFVAVAQRDYQAAERNLDRTMSAVTSKRRDRLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]