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The map label for this gene is dacA [H]

Identifier: 222093783

GI number: 222093783

Start: 17053

End: 18360

Strand: Direct

Name: dacA [H]

Synonym: BCQ_0015

Alternate gene names: 222093783

Gene position: 17053-18360 (Clockwise)

Preceding gene: 222093782

Following gene: 222093784

Centisome position: 0.33

GC content: 35.93

Gene sequence:

>1308_bases
ATGTTTTGCAAAAGATTCATTGCACTAGTAACAATGCTTACACTAGCTTGTAGTATGTTGTTACCATATAGCAATGCATC
AGCTGAAACAGGAGCCGCTTTAAATATTGAAGCAGGTGCGGCAATTTTAGTTGAAGCGAATTCTGGAAAAATTGTATATC
AAAAGAATGCAGATGAATTATTATCCATTGCTAGTATGACGAAGATGATGAGTGAGTATTTAGTTCACGAAGCAGTGGAT
AAAGGAAAGCTTAAGTGGGATCAGAAAATTAAGGTTTCTGAATATGCGTATAAAGTTTCACAGGATGCTTCATTGTCAAA
TGTGGCATTAGAAAATGGCGGCTCTTACACAGTAAAAGAGTTATACGAGGCAATGGCAATCTTCTCTGCAAATGGTGCAA
CGATTGCATTAGCAGAAGCAATTGCAGGTAAAGAAGTAGATTTCGTAAAAATGATGAATGATAAATCGAAAGAACTAGGG
TTGAAAAATTATAAATTTGTCAATTCTACAGGTTTAACGAATAAAGATTTAAAGGGAATGCATCCAGAAGGAACAACAGC
GGATGAAGAAAATAAAATGTCTGCAAAGGATGTTGCAACTTTAGCACAACATTTAATTAAAGATTATCCAAAAGTGTTAG
ATACAGCAAAAATCCCGAAAAAAGAATTCCGTCCAGAAAAAGAGAAGTTTGCAATGTCGAACTGGAACTGGATGTTAAAG
GGCTTAGTTAAAGAATATGATGGCGTAGATGGCCTAAAAACAGGCTCAACTCCAGAAGCGGGAGATTGCTTCACTGGTAC
GGTTGAAAGAAACGGTATGCGTTTTATTTCTGTAGTGATTAAAACAAGTTCTCATACAGCACGTTTTGATGAAACAAAGA
AGCTATATGATTATGGATTTGCTAACTTTGAAATGAAACAAATGTATAAAAAAGGTTCTTCAGTAAAAGGACAAGAAACA
GTACGAGTAGAAAATGCAAAAGACAAAGATGTAGCAGTTCAAACGAAACAAGCCATTTCACTTCCAGTACCAAAAGGAAG
TAAAGATGTTTATAAAACAGAATTAAAAGAATCAAGTAAGGGACAAGAGGCACCTATTAAAAAGGGAGCTGCGCTTGGTC
AAATGGTAATTACGCCAAAAGATACAAACGATCCTGGATTTTTAACTGGTAAGTCGTTGCAAGTGGATCTTGTAACAACA
GCTGAAGTAGAAGAAGCAAACTGGTTTACTCGTGCTATGCGCGGAATTGGTTCTTTCTTTAGTGGTATATGGAATAGTGC
TGTTGATACAGTAAAAGGTTGGTTTTAA

Upstream 100 bases:

>100_bases
AATAGACGGAGATTTTATATCTCTGTCTATTTTTTTTTGATTATGTTAGAATAACGGTTATGTGAGTACATAGATATTGG
GGGTAGCAAAAGTGAAAGGT

Downstream 100 bases:

>100_bases
AAGCTCCTCATTGTAGGGGCTTTTTTTTATTCCTATTTTTCATACCGACTTTATGAAAAAGTAGTAGACAAGCATCTGAT
AGTTAGTGGTAGAATGTAAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFCKRFIALVTMLTLACSMLLPYSNASAETGAALNIEAGAAILVEANSGKIVYQKNADELLSIASMTKMMSEYLVHEAVD
KGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSKELG
LKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNWMLK
GLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKGQET
VRVENAKDKDVAVQTKQAISLPVPKGSKDVYKTELKESSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDLVTT
AEVEEANWFTRAMRGIGSFFSGIWNSAVDTVKGWF

Sequences:

>Translated_435_residues
MFCKRFIALVTMLTLACSMLLPYSNASAETGAALNIEAGAAILVEANSGKIVYQKNADELLSIASMTKMMSEYLVHEAVD
KGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSKELG
LKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNWMLK
GLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKGQET
VRVENAKDKDVAVQTKQAISLPVPKGSKDVYKTELKESSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDLVTT
AEVEEANWFTRAMRGIGSFFSGIWNSAVDTVKGWF
>Mature_435_residues
MFCKRFIALVTMLTLACSMLLPYSNASAETGAALNIEAGAAILVEANSGKIVYQKNADELLSIASMTKMMSEYLVHEAVD
KGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSKELG
LKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNWMLK
GLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKGQET
VRVENAKDKDVAVQTKQAISLPVPKGSKDVYKTELKESSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDLVTT
AEVEEANWFTRAMRGIGSFFSGIWNSAVDTVKGWF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=420, Percent_Identity=30.2380952380952, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1786851, Length=438, Percent_Identity=28.0821917808219, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082022, Length=398, Percent_Identity=27.1356783919598, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082054, Length=298, Percent_Identity=25.8389261744966, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47810; Mature: 47810

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCKRFIALVTMLTLACSMLLPYSNASAETGAALNIEAGAAILVEANSGKIVYQKNADEL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCEEEEECCHHHH
LSIASMTKMMSEYLVHEAVDKGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCHHHEEECCCCCEEHHH
LYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSKELGLKNYKFVNSTGLTNKDLKGM
HHHHHHHHCCCCCEEEEHHHHCCCCCHHHHHHCCCHHHCCCCCEEEECCCCCCCCCCCCC
HPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNWMLK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCHHHHHH
GLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGF
HHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCC
ANFEMKQMYKKGSSVKGQETVRVENAKDKDVAVQTKQAISLPVPKGSKDVYKTELKESSK
CCHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEHHHHEECCCCCCCHHHHHHHHHHCCC
GQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDLVTTAEVEEANWFTRAMRGIGSFF
CCCCCHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
SGIWNSAVDTVKGWF
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MFCKRFIALVTMLTLACSMLLPYSNASAETGAALNIEAGAAILVEANSGKIVYQKNADEL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCEEEEECCHHHH
LSIASMTKMMSEYLVHEAVDKGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCHHHEEECCCCCEEHHH
LYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSKELGLKNYKFVNSTGLTNKDLKGM
HHHHHHHHCCCCCEEEEHHHHCCCCCHHHHHHCCCHHHCCCCCEEEECCCCCCCCCCCCC
HPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNWMLK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCHHHHHH
GLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGF
HHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCC
ANFEMKQMYKKGSSVKGQETVRVENAKDKDVAVQTKQAISLPVPKGSKDVYKTELKESSK
CCHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEHHHHEECCCCCCCHHHHHHHHHHCCC
GQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDLVTTAEVEEANWFTRAMRGIGSFF
CCCCCHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
SGIWNSAVDTVKGWF
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]