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The map label for this gene is cofH [H]

Identifier: 221636305

GI number: 221636305

Start: 881273

End: 882598

Strand: Reverse

Name: cofH [H]

Synonym: trd_A0899

Alternate gene names: 221636305

Gene position: 882598-881273 (Counterclockwise)

Preceding gene: 221636306

Following gene: 221636303

Centisome position: 96.17

GC content: 62.75

Gene sequence:

>1326_bases
ATGCACGAGACTCCTGCCTTGCATGAACTCCTCGAACGCCAGCTCGGACTCGCCTCGCCCGAAGTGGCTCGCATTCTCGA
TCGCGCTCTCTCCGGACATGAGCTGACGATCGAGGAGGGAGAGCGGCTCTTCGCGACGCATGGCCGGGATCTGTTTCTCG
TCACGCTGGTGGCCGACGAGCTCCGACGGCGTGCGGTCGGCGACGTCGTCACCTATGTCGTCAACCGGAACATCAATTTC
ACCAACGTCTGTATCAAGCGCTGCGGTTTCTGCGCCTTTTCCCGTGGCCATCGGGAAGAACAAGGCTACTTCCTGCCGCT
CGAGGAGATCGTTCGGCGAGCGCGTGAGGCCTGGGAACTCGGTGCCACCGAGGTCTGCATCCAGGCCGGGCTACCGCCCA
AGATGGATCCTGATCTCTACCCGACCATCTGTCGAGCGATCAAGGCCGAGCTGCCCGAGATCCACATTCACGGTTTCTCC
CCGGAGGAAGTCGTCTATGGCGCCTTGCGTGCTCGAACCTCTATCGAGGAGTATCTCCTCCGGCTCAAGGCAGCCGGCGT
CGGTTCCCTGCCTGGTACGTCGGCCGAGATCCTCGACGATACCGTGCGGCAACGCATTTCGCCGGGACGCATCACGACCG
CGCAGTGGGTGGAGGTGATCACGACGGCACACCGCCTCGGCATCCCGACGACGGCGACGATCATGTACGGACACGTGGAA
ACCGATCGCCAGCGGGCCGCTCACTTGGCTCTCATCCGGAGCATCCAAAAGGAGACCGGCGGGTTCACCGAGTTCGTGCC
GCTAAGCCTGGTCCACAGCGAAGCACCGATGTATCGCAAGCGCCTGCTTCCCGATCTTCGACCCGGCGCGACCGGTGAGG
ACGTCGTCAAGATGCATGCCATCGCTCGGATCATGCTTCACCCGTACATCCGGAACATCCAGGTGTCGTGGGTCAAAGAG
GGGCCGAAGCTGGCTCAGTGGTGTCTGAACGCTGGCGCGAACGATCTCGGTGGGACGCTGATCAATGAGAGTATTTCCAC
CGCAGCCGGCGCCACCTATGGCCAACTGGTACCACCGCGGGAGCTCCGTCGCTGGATCCGCGAGGCAGGTCGGGTTCCTG
CCGAACGGACGACGCTGTATGAAATCCGCACACCGTTGACCGATGAGGATCCCCCGCACCCCTTGGATGCCGCAGCGGAG
CATCCGGAGCGGTTCGGCTCCTATTTCCAACTGATCCAGGACGAGCGCTTCGCTTTCCGCAAGCACTACCGGATCGTGCT
CGATGCCATTCCTGCCCGTGCGGAAGCGGGAGCCGAAAAGCGGTGA

Upstream 100 bases:

>100_bases
TCCGCCGTGGTGAGGAGTTCCTCGATCCTGCGATCCGGAACCGGGTCGCGGCGCTCGTCGACCCAGGTGGACTGGTTCCC
CGGGAAAAGGAGTTGTGGTG

Downstream 100 bases:

>100_bases
TCGGTTAGGAGCGGTGACGGAGCGGGTCGTACACCAATCCTTGTCGCCACTCCTCCGGGTTGGCCGCAACGTGAGCTGCC
CAGTACTCGGCGACCTCTTG

Product: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MHETPALHELLERQLGLASPEVARILDRALSGHELTIEEGERLFATHGRDLFLVTLVADELRRRAVGDVVTYVVNRNINF
TNVCIKRCGFCAFSRGHREEQGYFLPLEEIVRRAREAWELGATEVCIQAGLPPKMDPDLYPTICRAIKAELPEIHIHGFS
PEEVVYGALRARTSIEEYLLRLKAAGVGSLPGTSAEILDDTVRQRISPGRITTAQWVEVITTAHRLGIPTTATIMYGHVE
TDRQRAAHLALIRSIQKETGGFTEFVPLSLVHSEAPMYRKRLLPDLRPGATGEDVVKMHAIARIMLHPYIRNIQVSWVKE
GPKLAQWCLNAGANDLGGTLINESISTAAGATYGQLVPPRELRRWIREAGRVPAERTTLYEIRTPLTDEDPPHPLDAAAE
HPERFGSYFQLIQDERFAFRKHYRIVLDAIPARAEAGAEKR

Sequences:

>Translated_441_residues
MHETPALHELLERQLGLASPEVARILDRALSGHELTIEEGERLFATHGRDLFLVTLVADELRRRAVGDVVTYVVNRNINF
TNVCIKRCGFCAFSRGHREEQGYFLPLEEIVRRAREAWELGATEVCIQAGLPPKMDPDLYPTICRAIKAELPEIHIHGFS
PEEVVYGALRARTSIEEYLLRLKAAGVGSLPGTSAEILDDTVRQRISPGRITTAQWVEVITTAHRLGIPTTATIMYGHVE
TDRQRAAHLALIRSIQKETGGFTEFVPLSLVHSEAPMYRKRLLPDLRPGATGEDVVKMHAIARIMLHPYIRNIQVSWVKE
GPKLAQWCLNAGANDLGGTLINESISTAAGATYGQLVPPRELRRWIREAGRVPAERTTLYEIRTPLTDEDPPHPLDAAAE
HPERFGSYFQLIQDERFAFRKHYRIVLDAIPARAEAGAEKR
>Mature_441_residues
MHETPALHELLERQLGLASPEVARILDRALSGHELTIEEGERLFATHGRDLFLVTLVADELRRRAVGDVVTYVVNRNINF
TNVCIKRCGFCAFSRGHREEQGYFLPLEEIVRRAREAWELGATEVCIQAGLPPKMDPDLYPTICRAIKAELPEIHIHGFS
PEEVVYGALRARTSIEEYLLRLKAAGVGSLPGTSAEILDDTVRQRISPGRITTAQWVEVITTAHRLGIPTTATIMYGHVE
TDRQRAAHLALIRSIQKETGGFTEFVPLSLVHSEAPMYRKRLLPDLRPGATGEDVVKMHAIARIMLHPYIRNIQVSWVKE
GPKLAQWCLNAGANDLGGTLINESISTAAGATYGQLVPPRELRRWIREAGRVPAERTTLYEIRTPLTDEDPPHPLDAAAE
HPERFGSYFQLIQDERFAFRKHYRIVLDAIPARAEAGAEKR

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 49466; Mature: 49466

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHETPALHELLERQLGLASPEVARILDRALSGHELTIEEGERLFATHGRDLFLVTLVADE
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHCCCEEEEECCCCEEEHHHHHHH
LRRRAVGDVVTYVVNRNINFTNVCIKRCGFCAFSRGHREEQGYFLPLEEIVRRAREAWEL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCCCCCCCCEECHHHHHHHHHHHHHC
GATEVCIQAGLPPKMDPDLYPTICRAIKAELPEIHIHGFSPEEVVYGALRARTSIEEYLL
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
RLKAAGVGSLPGTSAEILDDTVRQRISPGRITTAQWVEVITTAHRLGIPTTATIMYGHVE
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
TDRQRAAHLALIRSIQKETGGFTEFVPLSLVHSEAPMYRKRLLPDLRPGATGEDVVKMHA
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHH
IARIMLHPYIRNIQVSWVKEGPKLAQWCLNAGANDLGGTLINESISTAAGATYGQLVPPR
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHCCHHHHHHHHHHCCCCCCCCCCHH
ELRRWIREAGRVPAERTTLYEIRTPLTDEDPPHPLDAAAEHPERFGSYFQLIQDERFAFR
HHHHHHHHHCCCCCCHHEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
KHYRIVLDAIPARAEAGAEKR
HHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure
MHETPALHELLERQLGLASPEVARILDRALSGHELTIEEGERLFATHGRDLFLVTLVADE
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHCCCEEEEECCCCEEEHHHHHHH
LRRRAVGDVVTYVVNRNINFTNVCIKRCGFCAFSRGHREEQGYFLPLEEIVRRAREAWEL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCCCCCCCCEECHHHHHHHHHHHHHC
GATEVCIQAGLPPKMDPDLYPTICRAIKAELPEIHIHGFSPEEVVYGALRARTSIEEYLL
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
RLKAAGVGSLPGTSAEILDDTVRQRISPGRITTAQWVEVITTAHRLGIPTTATIMYGHVE
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
TDRQRAAHLALIRSIQKETGGFTEFVPLSLVHSEAPMYRKRLLPDLRPGATGEDVVKMHA
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHH
IARIMLHPYIRNIQVSWVKEGPKLAQWCLNAGANDLGGTLINESISTAAGATYGQLVPPR
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHCCHHHHHHHHHHCCCCCCCCCCHH
ELRRWIREAGRVPAERTTLYEIRTPLTDEDPPHPLDAAAEHPERFGSYFQLIQDERFAFR
HHHHHHHHHCCCCCCHHEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
KHYRIVLDAIPARAEAGAEKR
HHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]