| Definition | Thermomicrobium roseum DSM 5159 plasmid unnamed, complete sequence. |
|---|---|
| Accession | NC_011961 |
| Length | 917,738 |
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The map label for this gene is cofG [H]
Identifier: 221636306
GI number: 221636306
Start: 882598
End: 883812
Strand: Reverse
Name: cofG [H]
Synonym: trd_A0900
Alternate gene names: 221636306
Gene position: 883812-882598 (Counterclockwise)
Preceding gene: 221636308
Following gene: 221636305
Centisome position: 96.3
GC content: 66.17
Gene sequence:
>1215_bases GTGGTGATCTGCTTCCGGACGGCGAACGCGGTCGATCCGGCCATGGAGCGAGCCGTCGCCGACGCGGTCGATCGCGGTTG CCTGGATCCGGCTGCTGCTCGCCGCCTCATCGCAGCCGAGGCACCGCTGTTGCTGCTCCTCCTCGAAGCGGCCTCGCTCG TGCGTGAGCGCCGGTCCGGTCGGACGATCACCTACTCGCGCAAGGTCTTCCTTCCGCTCACGAATTTATGTCGTGATCAA TGTGCCTATTGTACGTTCGTGCGCCGGCCGAGCGATCCGCGTGCCCACACGATGACGCCGGAAGAAGTGCTCGCCGTCGC TGAGGCTGGCCGAGCGGCCGGATGCAAGGAAGCGCTCTTCAGCCTGGGGGACAAGCCGGAACTCCGCTATGCCTCCTATC GACAGTGGCTGGCGGCGCGCGGCTATTCCCGGACGATCGAGTATCTCCGGGACATGTGCGCGCTGGTTCTCGAACAGACG GGCCTGCTTCCTCATGCGAATCCCGGCGTGATGAGCGAGGACGATCTCGCGTTGCTCCGCCCGGTGACGGCCAGTATGGG TCTGATGCTCGAGTCGACGAGCGAGCGCCTCCTCGAGCGAGGGGGCGCGCACCGCGGGTGCCCCGATAAGGTGCCGGCTG TACGGCTGGCTACGCTGGAAGCGGCCGGCCGCCTCCGGGTGCCCTTCACGACCGGCATCCTCATCGGGATCGGCGAGACG CTGGAAGAGCGCGTCGAGGCCCTCTACGCGATCCGGGAGCTGCAGGATCGCTATGGTCACATCCAGGAAGTGATCGTTCA GAACTTCCGCCGCAAGCCGGATATCCGGATGCGGGATTGGCCAGAGCCGACCCTGCTCGACATGCTGCGGACAATCGCGG TCGCTCGCTTGATCCTCGGAACCACGACGGCAGTCCAGGCGCCGCCCAACCTGATGCCCGATGGCTACGAGGCCTATCTC CTCGCTGGACTCGACGACTGGGGTGGTATCTCACCGGTAACCCGCGATTACATCAACCCGGAGCGCGCCTGGCCGCATCT GCGCGAACTGAAGGCCCGCACCGAGGCGAGCGGGCTTCAGTTGCGCGAGCGCCTGGCCGTCTATCCGGGATTCATCCGCC GTGGTGAGGAGTTCCTCGATCCTGCGATCCGGAACCGGGTCGCGGCGCTCGTCGACCCAGGTGGACTGGTTCCCCGGGAA AAGGAGTTGTGGTGA
Upstream 100 bases:
>100_bases CGTGTGAAGGGAGTGCGGGTGCGCCGGGATGACTGGCATCCCGAGGAAGGCTGCGCCATACTACGAGCGCCTGCGGGCGC GCAGAGAGGGGGCGAGACCA
Downstream 100 bases:
>100_bases TGCACGAGACTCCTGCCTTGCATGAACTCCTCGAACGCCAGCTCGGACTCGCCTCGCCCGAAGTGGCTCGCATTCTCGAT CGCGCTCTCTCCGGACATGA
Product: FO synthase subunit 1
Products: Biotin [C]
Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase [H]
Number of amino acids: Translated: 404; Mature: 404
Protein sequence:
>404_residues MVICFRTANAVDPAMERAVADAVDRGCLDPAAARRLIAAEAPLLLLLLEAASLVRERRSGRTITYSRKVFLPLTNLCRDQ CAYCTFVRRPSDPRAHTMTPEEVLAVAEAGRAAGCKEALFSLGDKPELRYASYRQWLAARGYSRTIEYLRDMCALVLEQT GLLPHANPGVMSEDDLALLRPVTASMGLMLESTSERLLERGGAHRGCPDKVPAVRLATLEAAGRLRVPFTTGILIGIGET LEERVEALYAIRELQDRYGHIQEVIVQNFRRKPDIRMRDWPEPTLLDMLRTIAVARLILGTTTAVQAPPNLMPDGYEAYL LAGLDDWGGISPVTRDYINPERAWPHLRELKARTEASGLQLRERLAVYPGFIRRGEEFLDPAIRNRVAALVDPGGLVPRE KELW
Sequences:
>Translated_404_residues MVICFRTANAVDPAMERAVADAVDRGCLDPAAARRLIAAEAPLLLLLLEAASLVRERRSGRTITYSRKVFLPLTNLCRDQ CAYCTFVRRPSDPRAHTMTPEEVLAVAEAGRAAGCKEALFSLGDKPELRYASYRQWLAARGYSRTIEYLRDMCALVLEQT GLLPHANPGVMSEDDLALLRPVTASMGLMLESTSERLLERGGAHRGCPDKVPAVRLATLEAAGRLRVPFTTGILIGIGET LEERVEALYAIRELQDRYGHIQEVIVQNFRRKPDIRMRDWPEPTLLDMLRTIAVARLILGTTTAVQAPPNLMPDGYEAYL LAGLDDWGGISPVTRDYINPERAWPHLRELKARTEASGLQLRERLAVYPGFIRRGEEFLDPAIRNRVAALVDPGGLVPRE KELW >Mature_404_residues MVICFRTANAVDPAMERAVADAVDRGCLDPAAARRLIAAEAPLLLLLLEAASLVRERRSGRTITYSRKVFLPLTNLCRDQ CAYCTFVRRPSDPRAHTMTPEEVLAVAEAGRAAGCKEALFSLGDKPELRYASYRQWLAARGYSRTIEYLRDMCALVLEQT GLLPHANPGVMSEDDLALLRPVTASMGLMLESTSERLLERGGAHRGCPDKVPAVRLATLEAAGRLRVPFTTGILIGIGET LEERVEALYAIRELQDRYGHIQEVIVQNFRRKPDIRMRDWPEPTLLDMLRTIAVARLILGTTTAVQAPPNLMPDGYEAYL LAGLDDWGGISPVTRDYINPERAWPHLRELKARTEASGLQLRERLAVYPGFIRRGEEFLDPAIRNRVAALVDPGGLVPRE KELW
Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]
COG id: COG1060
COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the radical SAM superfamily. CofH family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013785 - InterPro: IPR019940 - InterPro: IPR006638 - InterPro: IPR019939 - InterPro: IPR020050 - InterPro: IPR007197 [H]
Pfam domain/function: PF04055 Radical_SAM [H]
EC number: 2.8.1.6 [C]
Molecular weight: Translated: 45033; Mature: 45033
Theoretical pI: Translated: 7.72; Mature: 7.72
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.0 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 2.0 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 4.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVICFRTANAVDPAMERAVADAVDRGCLDPAAARRLIAAEAPLLLLLLEAASLVRERRSG CEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC RTITYSRKVFLPLTNLCRDQCAYCTFVRRPSDPRAHTMTPEEVLAVAEAGRAAGCKEALF CEEEECCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH SLGDKPELRYASYRQWLAARGYSRTIEYLRDMCALVLEQTGLLPHANPGVMSEDDLALLR HCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH PVTASMGLMLESTSERLLERGGAHRGCPDKVPAVRLATLEAAGRLRVPFTTGILIGIGET HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEECCCHHHHHEECCHH LEERVEALYAIRELQDRYGHIQEVIVQNFRRKPDIRMRDWPEPTLLDMLRTIAVARLILG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH TTTAVQAPPNLMPDGYEAYLLAGLDDWGGISPVTRDYINPERAWPHLRELKARTEASGLQ CCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHH LRERLAVYPGFIRRGEEFLDPAIRNRVAALVDPGGLVPREKELW HHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCCCCCCCCCCCCC >Mature Secondary Structure MVICFRTANAVDPAMERAVADAVDRGCLDPAAARRLIAAEAPLLLLLLEAASLVRERRSG CEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC RTITYSRKVFLPLTNLCRDQCAYCTFVRRPSDPRAHTMTPEEVLAVAEAGRAAGCKEALF CEEEECCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH SLGDKPELRYASYRQWLAARGYSRTIEYLRDMCALVLEQTGLLPHANPGVMSEDDLALLR HCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH PVTASMGLMLESTSERLLERGGAHRGCPDKVPAVRLATLEAAGRLRVPFTTGILIGIGET HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEECCCHHHHHEECCHH LEERVEALYAIRELQDRYGHIQEVIVQNFRRKPDIRMRDWPEPTLLDMLRTIAVARLILG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH TTTAVQAPPNLMPDGYEAYLLAGLDDWGGISPVTRDYINPERAWPHLRELKARTEASGLQ CCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHH LRERLAVYPGFIRRGEEFLDPAIRNRVAALVDPGGLVPREKELW HHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Fe2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.002 {dethiobiotin}} [C]
Substrates: Dethiobiotin; sulfur [C]
Specific reaction: Dethiobiotin + sulfur = biotin [C]
General reaction: Sulfur atom transfer [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12000953 [H]