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The map label for this gene is paaF [C]

Identifier: 221636232

GI number: 221636232

Start: 810698

End: 811492

Strand: Reverse

Name: paaF [C]

Synonym: trd_A0826

Alternate gene names: 221636232

Gene position: 811492-810698 (Counterclockwise)

Preceding gene: 221636233

Following gene: 221636231

Centisome position: 88.42

GC content: 64.28

Gene sequence:

>795_bases
ATGACCGCGACCGAGCAGAGAACCTACCAGTACATCCTGACCGATGTGCGCGATGGGATCGCTGTGGTGACGATGAACCG
TCCGGAGAAGCGGAACGCGCTCTCGGTCGCGCACATGGAGGAGCTGACGGATGCCTTCCGGCGGATCGGTGAGGATCGGG
ACATCGCGGTCGCAATCTTGCGCGGTGCCGGGCCGATCTTCTGCGCTGGTCACGACTTGAACGAGCTGACCGGATGCGAT
ACGCCGACCGCGCGCCGTATCTTCGCGGTCTGTACGACGCTGATGCAGACGATCCGATCGATCCCCCAGCCGGTGATCGC
TCAAGTCCATGGGCTGGCGACTGCCGCGGGCTTCCAGTTGGCTGCGACCTGCGACCTCATCGTGGCGGCGGCCGGGACAC
GCTTCCAGACTCCAGGCGTGAAGATCGGGTTGTTCTGCTCGACTCCGATGGTGGCGGTGAGCCGGGTACTGCCGCCGAAG
AAGACGTTCGAGCTTTTGGTGACCGGTGAACCGATCACGGCGGAGGAGGCGGCGCAGCTGGGGATGGTGAACCGAGTCGT
CCCGGCTGAGGAACTGGAGGCTGCCACCTGGGAACTGGCGGAGAAGATCCGAGCAGCGAGCCGCTACGTGGTCGGGATCG
GTAAGCAAGCGTTCTACCGACAACTGGAGCTTCCTGAGCACCTCGCCTATGCCTACGCGATGGAAGTGATGGCGCTCAAC
GCGCTGGCAGCGGACGCGCAGGAGGGGATCTGCGCCTTCCTGGAAAAGCGCCAGCCACAGTGGCGCAATGCCTGA

Upstream 100 bases:

>100_bases
GCGAACTCGTCGGTGCCGGAACGGATGGGCGGCCCAGCGAGCCCGCAGACGACTGAGGGAACCGGAGCGCGAGCCGGCGA
GGCCGGAAGGAGGGACAACG

Downstream 100 bases:

>100_bases
CACCGACAGACGAGGTGACTCTGGCCGAGCCGTGGCGGTAACGATCGGGGCGAGCCGCGTGGTCGTCGTCGAGGGGCACG
ATCGGAGGTTCGCTCGCTGA

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTATEQRTYQYILTDVRDGIAVVTMNRPEKRNALSVAHMEELTDAFRRIGEDRDIAVAILRGAGPIFCAGHDLNELTGCD
TPTARRIFAVCTTLMQTIRSIPQPVIAQVHGLATAAGFQLAATCDLIVAAAGTRFQTPGVKIGLFCSTPMVAVSRVLPPK
KTFELLVTGEPITAEEAAQLGMVNRVVPAEELEAATWELAEKIRAASRYVVGIGKQAFYRQLELPEHLAYAYAMEVMALN
ALAADAQEGICAFLEKRQPQWRNA

Sequences:

>Translated_264_residues
MTATEQRTYQYILTDVRDGIAVVTMNRPEKRNALSVAHMEELTDAFRRIGEDRDIAVAILRGAGPIFCAGHDLNELTGCD
TPTARRIFAVCTTLMQTIRSIPQPVIAQVHGLATAAGFQLAATCDLIVAAAGTRFQTPGVKIGLFCSTPMVAVSRVLPPK
KTFELLVTGEPITAEEAAQLGMVNRVVPAEELEAATWELAEKIRAASRYVVGIGKQAFYRQLELPEHLAYAYAMEVMALN
ALAADAQEGICAFLEKRQPQWRNA
>Mature_263_residues
TATEQRTYQYILTDVRDGIAVVTMNRPEKRNALSVAHMEELTDAFRRIGEDRDIAVAILRGAGPIFCAGHDLNELTGCDT
PTARRIFAVCTTLMQTIRSIPQPVIAQVHGLATAAGFQLAATCDLIVAAAGTRFQTPGVKIGLFCSTPMVAVSRVLPPKK
TFELLVTGEPITAEEAAQLGMVNRVVPAEELEAATWELAEKIRAASRYVVGIGKQAFYRQLELPEHLAYAYAMEVMALNA
LAADAQEGICAFLEKRQPQWRNA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=46.2151394422311, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=26.7924528301887, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI260274832, Length=258, Percent_Identity=23.6434108527132, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI45643119, Length=258, Percent_Identity=23.6434108527132, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI260275230, Length=258, Percent_Identity=23.6434108527132, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI70995211, Length=183, Percent_Identity=31.1475409836066, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=29.6, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=187, Percent_Identity=25.668449197861, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI51538581, Length=187, Percent_Identity=25.668449197861, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI25453481, Length=187, Percent_Identity=25.668449197861, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI51538573, Length=187, Percent_Identity=25.668449197861, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4757968, Length=187, Percent_Identity=25.668449197861, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI49355787, Length=187, Percent_Identity=25.668449197861, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=31.0483870967742, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=33.596837944664, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=28.8389513108614, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=29.8449612403101, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=30.9236947791165, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=30.9178743961353, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=24.8618784530387, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=40.7563025210084, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=29.4354838709677, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=262, Percent_Identity=27.0992366412214, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=27.6923076923077, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=196, Percent_Identity=32.1428571428571, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=191, Percent_Identity=29.8429319371728, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=240, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=201, Percent_Identity=23.8805970149254, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=38.8888888888889, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=29.6992481203008, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=29.6992481203008, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=31.2741312741313, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24654903, Length=195, Percent_Identity=28.2051282051282, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357171, Length=265, Percent_Identity=27.9245283018868, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24650670, Length=178, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=26.8181818181818, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28789; Mature: 28657

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATEQRTYQYILTDVRDGIAVVTMNRPEKRNALSVAHMEELTDAFRRIGEDRDIAVAIL
CCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
RGAGPIFCAGHDLNELTGCDTPTARRIFAVCTTLMQTIRSIPQPVIAQVHGLATAAGFQL
ECCCCEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHH
AATCDLIVAAAGTRFQTPGVKIGLFCSTPMVAVSRVLPPKKTFELLVTGEPITAEEAAQL
HHHHHHHHHCCCCEECCCCEEEEEEECCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
GMVNRVVPAEELEAATWELAEKIRAASRYVVGIGKQAFYRQLELPEHLAYAYAMEVMALN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCHHHHHHHHHHHHHHHH
ALAADAQEGICAFLEKRQPQWRNA
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TATEQRTYQYILTDVRDGIAVVTMNRPEKRNALSVAHMEELTDAFRRIGEDRDIAVAIL
CCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
RGAGPIFCAGHDLNELTGCDTPTARRIFAVCTTLMQTIRSIPQPVIAQVHGLATAAGFQL
ECCCCEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHH
AATCDLIVAAAGTRFQTPGVKIGLFCSTPMVAVSRVLPPKKTFELLVTGEPITAEEAAQL
HHHHHHHHHCCCCEECCCCEEEEEEECCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
GMVNRVVPAEELEAATWELAEKIRAASRYVVGIGKQAFYRQLELPEHLAYAYAMEVMALN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCHHHHHHHHHHHHHHHH
ALAADAQEGICAFLEKRQPQWRNA
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA