Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 221636233

GI number: 221636233

Start: 811537

End: 813456

Strand: Reverse

Name: mdeA [H]

Synonym: trd_A0827

Alternate gene names: 221636233

Gene position: 813456-811537 (Counterclockwise)

Preceding gene: 221636234

Following gene: 221636232

Centisome position: 88.64

GC content: 65.78

Gene sequence:

>1920_bases
GTGCTGGTCGGGCACACCGGTAGAACGAGCGAGGGAGGAACCAGGGTGGCGAGCGAGCGAGATCCGTTTCGGAACTATGC
GATCGAGACACTGGCTGTGCATGCCGGGCAAGCGCCCGATCCAGCCACGGGAGCGCGGGCGGTTCCCATCTATCAGACGA
CATCGTTCGTCTTCCACGATGCCGACCACGCCTCGCAACTGTTCAATCTGGATGAGCCTGGACATATCTACACGCGGATC
AGCAACCCGACGGTCGAGGTCTTCGAAAAGCGGGTGGCGTACCTGGAAGGAGGGGTGGCGGCGGTCGCGACGGCGAGCGG
TCAAGCGGCGACCACGCTGGCGATCCTGAACATCGCGCGGGCCGGTGACGAGGTCGTGGCGAGCACGAGCCTGTACGGGG
GAACGGTCGCGCTGTTCGCCAACACGCTCTCTGACCTGGGGATCACGGTCCGGTTCGTCGAGCCGTCCGATCCGGAGAAT
TTCCGACGAGCGATCACCGAGCGAACGAAGTGCATCTACGCTGAGACGATCGGGAACCCGCGGCTCGACGTACTGGACAT
CGAGGCGGTGGCGCGCATCGCGCACGAGCATGGAATCCCGTTGATCATCGACAACACGTTCGCGACACCCTACCTGTGCC
GCCCGATCGAGTGGGGCGCGGACATCGTGGTGCATTCGGCGACCAAGTGGATCGGCGGGCACGGCATCGCGATCGGTGGC
GTGGTGGTCGATGCTGGCCGTTTCGACTGGGGGAACGGCAAGTTCCCGCGACTCGTCGAGCCGGATCCAGGCTACCACGG
GATCTCCTACTGGAAGGATTTCGGCAGCTTGGCCTATGTGACCAAGCTGCGGGCGCACATGATGCGCGACGTGGGCGCTT
GCATGAGCCCGTTCAATGCGTTCCTGCTCCTAGTGGGACTGGAGACGCTGCATGTGCGGATGGACCGGCATTGCGCCAAC
GCGCTGGAGATCGCGCGCTGGCTAGCCAGGCATCCGGCCGTGGCCTGGGTGGCCTACCCGGGCTTGCCAGAGCACCCGTC
GCATCACCTGGCGCGCAAGTACTTGCGGGAAGGGTGCTATGGCGCGGTCGTCGTCTTCGGCGTGAAGGGTGGGATGGAAG
CCGGCATCAAGGTCATCGACAACGTGGCGCTGTGGTCGCACCTGGCGAACGTGGGCGATGCCAAGTCGCTGATCATCCAT
CCGGCGAGCACCACGCACCGGCAGCTGACTCCCGAGCAACGAGTGGCAGCTGGGGTGGGGGATGACTTGATCCGGCTGGC
AGTCGGCATCGAGCATGTCGAGGATCTGAAGGCTGACCTGGATCGGGCCTTGCGGATCGCGACTGGCCTGGCAGCAGGTG
GTGACGGCCGAGCGCGCGAAGAGGTGTCGCAGGTCGTGCTCAACGACGAGCGGGTGATCCGCTCGGTGTGCACGCGGGCC
TTCGTGGAAGAGAACGGAACCCGTCGGCCGCTCGTCCTGGCGATCGTCGGGCTTTCCAATGACCCGGCTCGTCCCAGCTA
TCGCGTGGCGCAGAAACTCAAGCGGCTCGGCTACCGGATCATCCCGGTCAACCCGCTCATCGACGAAGCGCTCGGCGAGC
GGGCGTACTCCTCGCTCCGCGAACTCCCCGAACGGGCTGATGTCGTGGTGGTCTTCCGGGCGAGCGAGCATGCACCCGCC
GTCGCCCGCGAGGCAGTCGAGCACGGCGCACGGGTATTGTGGTTGCAAGAGGGTGTGGTCAGCCCGGAGGCGGCACGGAT
CGCGACGGAGGGCGGCCTGGCAGTGGTGATGAACCGCTGCATTTACAAGGAGGCACAGCGCTGGCGGGGCCACGCCAAGA
CGTTCCGACCGGACGATGTGGATCGCGAACTCGTCGGTGCCGGAACGGATGGGCGGCCCAGCGAGCCCGCAGACGACTGA

Upstream 100 bases:

>100_bases
TTGGCTCGCCGGCGGGCGGCGCTGTGGTCGGTCATTGGGCTGAGCCAGCGACGCGCTGGTGCGAGACGCAGGGGGTAAGA
TTCGAGCAGGCTGGCTCGGC

Downstream 100 bases:

>100_bases
GGGAACCGGAGCGCGAGCCGGCGAGGCCGGAAGGAGGGACAACGATGACCGCGACCGAGCAGAGAACCTACCAGTACATC
CTGACCGATGTGCGCGATGG

Product: O-acetylhomoserine (thiol)-lyase (O-acetylhomoserinesulfhydrylase) (OAH sulfhydrylase) (Homocysteine synthase)

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MLVGHTGRTSEGGTRVASERDPFRNYAIETLAVHAGQAPDPATGARAVPIYQTTSFVFHDADHASQLFNLDEPGHIYTRI
SNPTVEVFEKRVAYLEGGVAAVATASGQAATTLAILNIARAGDEVVASTSLYGGTVALFANTLSDLGITVRFVEPSDPEN
FRRAITERTKCIYAETIGNPRLDVLDIEAVARIAHEHGIPLIIDNTFATPYLCRPIEWGADIVVHSATKWIGGHGIAIGG
VVVDAGRFDWGNGKFPRLVEPDPGYHGISYWKDFGSLAYVTKLRAHMMRDVGACMSPFNAFLLLVGLETLHVRMDRHCAN
ALEIARWLARHPAVAWVAYPGLPEHPSHHLARKYLREGCYGAVVVFGVKGGMEAGIKVIDNVALWSHLANVGDAKSLIIH
PASTTHRQLTPEQRVAAGVGDDLIRLAVGIEHVEDLKADLDRALRIATGLAAGGDGRAREEVSQVVLNDERVIRSVCTRA
FVEENGTRRPLVLAIVGLSNDPARPSYRVAQKLKRLGYRIIPVNPLIDEALGERAYSSLRELPERADVVVVFRASEHAPA
VAREAVEHGARVLWLQEGVVSPEAARIATEGGLAVVMNRCIYKEAQRWRGHAKTFRPDDVDRELVGAGTDGRPSEPADD

Sequences:

>Translated_639_residues
MLVGHTGRTSEGGTRVASERDPFRNYAIETLAVHAGQAPDPATGARAVPIYQTTSFVFHDADHASQLFNLDEPGHIYTRI
SNPTVEVFEKRVAYLEGGVAAVATASGQAATTLAILNIARAGDEVVASTSLYGGTVALFANTLSDLGITVRFVEPSDPEN
FRRAITERTKCIYAETIGNPRLDVLDIEAVARIAHEHGIPLIIDNTFATPYLCRPIEWGADIVVHSATKWIGGHGIAIGG
VVVDAGRFDWGNGKFPRLVEPDPGYHGISYWKDFGSLAYVTKLRAHMMRDVGACMSPFNAFLLLVGLETLHVRMDRHCAN
ALEIARWLARHPAVAWVAYPGLPEHPSHHLARKYLREGCYGAVVVFGVKGGMEAGIKVIDNVALWSHLANVGDAKSLIIH
PASTTHRQLTPEQRVAAGVGDDLIRLAVGIEHVEDLKADLDRALRIATGLAAGGDGRAREEVSQVVLNDERVIRSVCTRA
FVEENGTRRPLVLAIVGLSNDPARPSYRVAQKLKRLGYRIIPVNPLIDEALGERAYSSLRELPERADVVVVFRASEHAPA
VAREAVEHGARVLWLQEGVVSPEAARIATEGGLAVVMNRCIYKEAQRWRGHAKTFRPDDVDRELVGAGTDGRPSEPADD
>Mature_639_residues
MLVGHTGRTSEGGTRVASERDPFRNYAIETLAVHAGQAPDPATGARAVPIYQTTSFVFHDADHASQLFNLDEPGHIYTRI
SNPTVEVFEKRVAYLEGGVAAVATASGQAATTLAILNIARAGDEVVASTSLYGGTVALFANTLSDLGITVRFVEPSDPEN
FRRAITERTKCIYAETIGNPRLDVLDIEAVARIAHEHGIPLIIDNTFATPYLCRPIEWGADIVVHSATKWIGGHGIAIGG
VVVDAGRFDWGNGKFPRLVEPDPGYHGISYWKDFGSLAYVTKLRAHMMRDVGACMSPFNAFLLLVGLETLHVRMDRHCAN
ALEIARWLARHPAVAWVAYPGLPEHPSHHLARKYLREGCYGAVVVFGVKGGMEAGIKVIDNVALWSHLANVGDAKSLIIH
PASTTHRQLTPEQRVAAGVGDDLIRLAVGIEHVEDLKADLDRALRIATGLAAGGDGRAREEVSQVVLNDERVIRSVCTRA
FVEENGTRRPLVLAIVGLSNDPARPSYRVAQKLKRLGYRIIPVNPLIDEALGERAYSSLRELPERADVVVVFRASEHAPA
VAREAVEHGARVLWLQEGVVSPEAARIATEGGLAVVMNRCIYKEAQRWRGHAKTFRPDDVDRELVGAGTDGRPSEPADD

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=428, Percent_Identity=36.4485981308411, Blast_Score=224, Evalue=3e-58,
Organism=Homo sapiens, GI299473759, Length=428, Percent_Identity=33.1775700934579, Blast_Score=183, Evalue=5e-46,
Organism=Homo sapiens, GI25453487, Length=427, Percent_Identity=31.615925058548, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=32.1428571428571, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789383, Length=420, Percent_Identity=26.9047619047619, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI87081810, Length=110, Percent_Identity=48.1818181818182, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71980708, Length=404, Percent_Identity=32.9207920792079, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=35.2245862884161, Blast_Score=216, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=34.9765258215962, Blast_Score=206, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=50.5773672055427, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319307, Length=437, Percent_Identity=34.0961098398169, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=454, Percent_Identity=25.9911894273128, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=367, Percent_Identity=26.7029972752044, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=428, Percent_Identity=26.1682242990654, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=31.5165876777251, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 69413; Mature: 69413

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVGHTGRTSEGGTRVASERDPFRNYAIETLAVHAGQAPDPATGARAVPIYQTTSFVFHD
CEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCEEEEEEEECEEEEEC
ADHASQLFNLDEPGHIYTRISNPTVEVFEKRVAYLEGGVAAVATASGQAATTLAILNIAR
CHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHH
AGDEVVASTSLYGGTVALFANTLSDLGITVRFVEPSDPENFRRAITERTKCIYAETIGNP
CCCHHEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCC
RLDVLDIEAVARIAHEHGIPLIIDNTFATPYLCRPIEWGADIVVHSATKWIGGHGIAIGG
CEEEEEHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCCCEEEECHHHHHCCCCEEEEE
VVVDAGRFDWGNGKFPRLVEPDPGYHGISYWKDFGSLAYVTKLRAHMMRDVGACMSPFNA
EEEECCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLLVGLETLHVRMDRHCANALEIARWLARHPAVAWVAYPGLPEHPSHHLARKYLREGCY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCC
GAVVVFGVKGGMEAGIKVIDNVALWSHLANVGDAKSLIIHPASTTHRQLTPEQRVAAGVG
EEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHCCC
DDLIRLAVGIEHVEDLKADLDRALRIATGLAAGGDGRAREEVSQVVLNDERVIRSVCTRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
FVEENGTRRPLVLAIVGLSNDPARPSYRVAQKLKRLGYRIIPVNPLIDEALGERAYSSLR
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
ELPERADVVVVFRASEHAPAVAREAVEHGARVLWLQEGVVSPEAARIATEGGLAVVMNRC
HCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHEECCCCHHHHHHHH
IYKEAQRWRGHAKTFRPDDVDRELVGAGTDGRPSEPADD
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLVGHTGRTSEGGTRVASERDPFRNYAIETLAVHAGQAPDPATGARAVPIYQTTSFVFHD
CEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCCCCEEEEEEEECEEEEEC
ADHASQLFNLDEPGHIYTRISNPTVEVFEKRVAYLEGGVAAVATASGQAATTLAILNIAR
CHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHH
AGDEVVASTSLYGGTVALFANTLSDLGITVRFVEPSDPENFRRAITERTKCIYAETIGNP
CCCHHEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCC
RLDVLDIEAVARIAHEHGIPLIIDNTFATPYLCRPIEWGADIVVHSATKWIGGHGIAIGG
CEEEEEHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCCCEEEECHHHHHCCCCEEEEE
VVVDAGRFDWGNGKFPRLVEPDPGYHGISYWKDFGSLAYVTKLRAHMMRDVGACMSPFNA
EEEECCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLLVGLETLHVRMDRHCANALEIARWLARHPAVAWVAYPGLPEHPSHHLARKYLREGCY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCC
GAVVVFGVKGGMEAGIKVIDNVALWSHLANVGDAKSLIIHPASTTHRQLTPEQRVAAGVG
EEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHCCC
DDLIRLAVGIEHVEDLKADLDRALRIATGLAAGGDGRAREEVSQVVLNDERVIRSVCTRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
FVEENGTRRPLVLAIVGLSNDPARPSYRVAQKLKRLGYRIIPVNPLIDEALGERAYSSLR
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
ELPERADVVVVFRASEHAPAVAREAVEHGARVLWLQEGVVSPEAARIATEGGLAVVMNRC
HCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHEECCCCHHHHHHHH
IYKEAQRWRGHAKTFRPDDVDRELVGAGTDGRPSEPADD
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]