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The map label for this gene is gatA [H]

Identifier: 221633729

GI number: 221633729

Start: 1761260

End: 1762729

Strand: Reverse

Name: gatA [H]

Synonym: trd_1756

Alternate gene names: 221633729

Gene position: 1762729-1761260 (Counterclockwise)

Preceding gene: 221633730

Following gene: 221633728

Centisome position: 88.0

GC content: 64.69

Gene sequence:

>1470_bases
GTGACCAGCGAACTCGTTCGCCTGACCGCTGTGGAGGCCCGCGCGCTGCTCGATCGACGCGAGATCAGCGCTCTGGAATT
GCTCGAGGCGCATCTCGCCCAGATCGAGCGACTGGAGCCTACTCTCCACGCCTTCATCACGGTCGCGCCGGAACTGGCGC
GTCGCCAAGCTCGGGCAGCTGACGACCGCCTCGCTCGCGGTGAGGGTGATCTCTTGACGGGTATACCGGTCGCACTGAAG
GACGTCCTCTGTACGGTCGATGCGCCGACGACAGCTGGGTCACGCATTCTGCAAGGATTCCGCTCGCCCTACGATGCGAC
CGTCGTCGCTCGCCTGCGGGCACAGGATGCGGTGTTCGTCGGCAAAACCAACACTGACGAGTTCGCCATGGGTTCCTCCA
CCGAGAACTCGGCCTACGGTCCGACCCGTAACCCGTGGGATCCGGACCGCGTTCCGGGCGGATCGAGTGGCGGTTCGGCA
GCAGCGGTCGCCGCTGGAGAAGCGATGGTTGCTCTCGGGTCGGATACGGGTGGATCGATCCGGCAGCCAGCGGGCTTCTG
CGGTATCGTCGGGCTCAAGCCGACGTATGGTCGAGTGTCCCGCTATGGACTCGTCGCTTTCGCGTCAAGCCTGGACCAGA
TCGGGCCCTTCGGCCGCACGGTCGCCGATGTGGCGATGCTGCTGCAGGTGATCGCCGGTCACGATCCAGCCGACTCCACA
TCGGTCCCCCTCCCGGTGCCCGATTATCGAGCGGCGCTGACCGGTGATATCCGTGGCCTGCGGATCGGTATCCCTGCCGA
GTACCGAGTGCCCGGAATGGATCAGGCAGTCGAGCGAGTGATCGAGGATGCCCTGGCGCTCCTGCAAGAACTGGGAGCCG
AGCTCGTTCCCGTGTCCTTGCCGCACACCGAATATGCCTTGGCCACGTACTACATCATCGCGCCCTCCGAGGCGAGCGCG
AACCTGGCACGCTATGACGGCGTGAAGTACGGGTTGAGCTTGCCCGGCGAAACGCTCTTGGAGCGCTACCTCCGCACGCG
CGGGGAAGGATTTGGGCCAGAAGTCAAGCGGCGCATCATGCTCGGCACCTACGCCTTGAGCGCTGGCTATTACGATGCCT
ATTACGTCAAGGCTCAAAAGGTGCGGACACTCATCAAACGCGACTTCGACGAAGCGTTCGCTCGTGTCGATGTGATCGCG
GCACCGACCTCGCCGACGGTGGCTTTCCACCTCGGCGAGCGCACGGCGGATCCGTTGCAGATGTACCTTTCCGATGTGTT
CACCATCCCGGCGAACATGGCTGGCCTCCCGGCAATCGCGGTTCCCTGTGGTTTCGCGCACGCCTTGCCGGTCAGTCTGC
AGTTCATGGGCCGGCCATTCGACGAGCCCACGCTCTTGCGGGTCGCCCACGCCTACGAGCAGGCCACCGACTGGCATACC
CGGTGGCCACCCCTGGTGTTCGGGGGCTGA

Upstream 100 bases:

>100_bases
GCCATCCCTGCCGGTGGAAGCTGTCCTGGCCAACGCTCCGGAAAGCGAAGATGGCTTCTTCCGGGTCCATGCGGTGCTCG
AGCAGAGTTGAGGGGGCACT

Downstream 100 bases:

>100_bases
GCGATGCTCTGGATCGGTTCCTGGCTCCGCACCGCGGTGCTGGTCGCTCTCGGCTTCGCGATCGCGCTCTATTTCGTCGT
CGCTTTCGGTCAACAAGCCT

Product: glutamyl-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTSELVRLTAVEARALLDRREISALELLEAHLAQIERLEPTLHAFITVAPELARRQARAADDRLARGEGDLLTGIPVALK
DVLCTVDAPTTAGSRILQGFRSPYDATVVARLRAQDAVFVGKTNTDEFAMGSSTENSAYGPTRNPWDPDRVPGGSSGGSA
AAVAAGEAMVALGSDTGGSIRQPAGFCGIVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVADVAMLLQVIAGHDPADST
SVPLPVPDYRAALTGDIRGLRIGIPAEYRVPGMDQAVERVIEDALALLQELGAELVPVSLPHTEYALATYYIIAPSEASA
NLARYDGVKYGLSLPGETLLERYLRTRGEGFGPEVKRRIMLGTYALSAGYYDAYYVKAQKVRTLIKRDFDEAFARVDVIA
APTSPTVAFHLGERTADPLQMYLSDVFTIPANMAGLPAIAVPCGFAHALPVSLQFMGRPFDEPTLLRVAHAYEQATDWHT
RWPPLVFGG

Sequences:

>Translated_489_residues
MTSELVRLTAVEARALLDRREISALELLEAHLAQIERLEPTLHAFITVAPELARRQARAADDRLARGEGDLLTGIPVALK
DVLCTVDAPTTAGSRILQGFRSPYDATVVARLRAQDAVFVGKTNTDEFAMGSSTENSAYGPTRNPWDPDRVPGGSSGGSA
AAVAAGEAMVALGSDTGGSIRQPAGFCGIVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVADVAMLLQVIAGHDPADST
SVPLPVPDYRAALTGDIRGLRIGIPAEYRVPGMDQAVERVIEDALALLQELGAELVPVSLPHTEYALATYYIIAPSEASA
NLARYDGVKYGLSLPGETLLERYLRTRGEGFGPEVKRRIMLGTYALSAGYYDAYYVKAQKVRTLIKRDFDEAFARVDVIA
APTSPTVAFHLGERTADPLQMYLSDVFTIPANMAGLPAIAVPCGFAHALPVSLQFMGRPFDEPTLLRVAHAYEQATDWHT
RWPPLVFGG
>Mature_488_residues
TSELVRLTAVEARALLDRREISALELLEAHLAQIERLEPTLHAFITVAPELARRQARAADDRLARGEGDLLTGIPVALKD
VLCTVDAPTTAGSRILQGFRSPYDATVVARLRAQDAVFVGKTNTDEFAMGSSTENSAYGPTRNPWDPDRVPGGSSGGSAA
AVAAGEAMVALGSDTGGSIRQPAGFCGIVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVADVAMLLQVIAGHDPADSTS
VPLPVPDYRAALTGDIRGLRIGIPAEYRVPGMDQAVERVIEDALALLQELGAELVPVSLPHTEYALATYYIIAPSEASAN
LARYDGVKYGLSLPGETLLERYLRTRGEGFGPEVKRRIMLGTYALSAGYYDAYYVKAQKVRTLIKRDFDEAFARVDVIAA
PTSPTVAFHLGERTADPLQMYLSDVFTIPANMAGLPAIAVPCGFAHALPVSLQFMGRPFDEPTLLRVAHAYEQATDWHTR
WPPLVFGG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=39.1566265060241, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI195972892, Length=505, Percent_Identity=25.7425742574257, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI166795287, Length=250, Percent_Identity=34, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=39.8633257403189, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=25.3493013972056, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71990152, Length=312, Percent_Identity=32.0512820512821, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=286, Percent_Identity=30.0699300699301, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=25.8169934640523, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=309, Percent_Identity=30.42071197411, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=309, Percent_Identity=30.42071197411, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=247, Percent_Identity=28.3400809716599, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=429, Percent_Identity=36.1305361305361, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=472, Percent_Identity=30.2966101694915, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=246, Percent_Identity=28.8617886178862, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648113, Length=452, Percent_Identity=43.8053097345133, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=26.2745098039216, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652985, Length=480, Percent_Identity=26.25, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922090, Length=480, Percent_Identity=26.25, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24652981, Length=480, Percent_Identity=26.25, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24652983, Length=480, Percent_Identity=26.25, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=28.3203125, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45550774, Length=252, Percent_Identity=33.7301587301587, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=252, Percent_Identity=33.7301587301587, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=252, Percent_Identity=33.7301587301587, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=485, Percent_Identity=25.1546391752577, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648441, Length=175, Percent_Identity=37.7142857142857, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648439, Length=175, Percent_Identity=37.7142857142857, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52454; Mature: 52323

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSELVRLTAVEARALLDRREISALELLEAHLAQIERLEPTLHAFITVAPELARRQARAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHH
DDRLARGEGDLLTGIPVALKDVLCTVDAPTTAGSRILQGFRSPYDATVVARLRAQDAVFV
HHHHHCCCCCEEECCHHHHHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCEEEE
GKTNTDEFAMGSSTENSAYGPTRNPWDPDRVPGGSSGGSAAAVAAGEAMVALGSDTGGSI
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCC
RQPAGFCGIVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVADVAMLLQVIAGHDPADST
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
SVPLPVPDYRAALTGDIRGLRIGIPAEYRVPGMDQAVERVIEDALALLQELGAELVPVSL
CCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PHTEYALATYYIIAPSEASANLARYDGVKYGLSLPGETLLERYLRTRGEGFGPEVKRRIM
CCCCEEEEEEEEEECCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
LGTYALSAGYYDAYYVKAQKVRTLIKRDFDEAFARVDVIAAPTSPTVAFHLGERTADPLQ
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCHHHH
MYLSDVFTIPANMAGLPAIAVPCGFAHALPVSLQFMGRPFDEPTLLRVAHAYEQATDWHT
HHHHHHHHCCCCCCCCCCHHHCCCCHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
RWPPLVFGG
CCCCCEECC
>Mature Secondary Structure 
TSELVRLTAVEARALLDRREISALELLEAHLAQIERLEPTLHAFITVAPELARRQARAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHH
DDRLARGEGDLLTGIPVALKDVLCTVDAPTTAGSRILQGFRSPYDATVVARLRAQDAVFV
HHHHHCCCCCEEECCHHHHHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCEEEE
GKTNTDEFAMGSSTENSAYGPTRNPWDPDRVPGGSSGGSAAAVAAGEAMVALGSDTGGSI
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCC
RQPAGFCGIVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVADVAMLLQVIAGHDPADST
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
SVPLPVPDYRAALTGDIRGLRIGIPAEYRVPGMDQAVERVIEDALALLQELGAELVPVSL
CCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PHTEYALATYYIIAPSEASANLARYDGVKYGLSLPGETLLERYLRTRGEGFGPEVKRRIM
CCCCEEEEEEEEEECCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
LGTYALSAGYYDAYYVKAQKVRTLIKRDFDEAFARVDVIAAPTSPTVAFHLGERTADPLQ
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCHHHH
MYLSDVFTIPANMAGLPAIAVPCGFAHALPVSLQFMGRPFDEPTLLRVAHAYEQATDWHT
HHHHHHHHCCCCCCCCCCHHHCCCCHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
RWPPLVFGG
CCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA