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The map label for this gene is glnA [H]

Identifier: 221633534

GI number: 221633534

Start: 1538062

End: 1539510

Strand: Reverse

Name: glnA [H]

Synonym: trd_1558

Alternate gene names: 221633534

Gene position: 1539510-1538062 (Counterclockwise)

Preceding gene: 221633536

Following gene: 221633533

Centisome position: 76.86

GC content: 59.35

Gene sequence:

>1449_bases
ATGTACGGGTCGTGCACATCGCCAGAACACGTTCTCGATCTGATCCGGTCACGTGGTATCCAGATCGTCGATTTGCGCTT
TACGGATCTTCCCGGCACGTGGCAGCATGCGTCGCTGCCAGCGAGCCAGCTCAGCTTGGCCGATTTCGAGCGCGGCATCG
GCTTCGATGGTTCGAGTATCCGCGGTTTCCAGGCCATCGAAGAAAGCGACATGTTGCTCGTTCCTGACCCGACGACGGCC
TTCATCGATCCGTTCGCGGCTGTCCCGACTCTGGTCATGATTTGTAACGTCGTCGATCCGGAGACGCGTCAACCGTATGC
GCGTGATCCGCGCTACGTTGCCCAGAAAGCCGAGCGATACCTGCGCGATCTGGGGATCGCCGACACGAGTTTCTGGGGAC
CGGAAATCGAGTTTTTCATTTTTGACCACGTCAGTTACGAGGTCTTGCCCTATCGCATGGGCTTCGCGATCGACTCGCGT
GAAGGGTACTGGAACAGTGGTCGCAACGACGGGACGCCGAATCTCGGCTATACCATCCGCCCCAAGGAGGGATACTTCCC
AGCTCCTCCTCACGATACCTTTCAGGACATCCGCAGCGAGGCGGCGTTGCTCCTCGAGCAGATCGGTGTTCCCGTCGAGA
TGCACCATCACGAAGTCGCGACCGCTGGCCAAGGCGAGATCGACATGCGGCGCGATACGTTGACTGCCATGGCCGACAAG
GTGATGATCTACAAGTATGTCATCAAGAACGTTGCCCGAAAGCATGGCTGCACAGCGACCTTCATGCCAAAGCCGCTCTT
CGGGGATAACGGCAACGGCATGCACACGCATCAGAGCTTGTGGCGCGATGGAACGAACCTGTTCTACGCGGCGTGCCATT
ATGCTGAACTCTCCGAGCTCGCCCTTTCCTACATCGCCGGCTTGCTGACCCATGTCGATGCGCTCCTCGCATTGACGAGC
CCGACGACCAACTCGTACAAGCGGCTCGTGCCGCACTACGAGGCTCCGGTCAACGTGGCCTTCTCCAAGCGCAATCGCAG
CGCTTGCATCCGGATCCCGATGTATACGACCGGACCGGAGAATGCTGCGGCCAAGCGGGTCGAGTTCCGCCCTCCCGACC
CGAGTTGCAATCCGTATCTGGCCTTCGCTGCCATGCTGATGGCCGGTCTGGATGGCATTCAGCGGCGACTCGATGCGAGC
CGATTTGGCCCGCTCGACGTCAATACCTATCAGTTGCCGCCCGAGCAGGCAGCACAGGTCAAGAGCGTGCCGGGCTCGCT
CGGGGCCGCCCTCGAGGCGCTCGAGCGTGATCATGACTGGCTGCTGGAAGGGAACGTCTTCACCCGTGATCTCTTGGAAA
CCTGGGTGAGCTATAAGCGCGAGAAGGAACTCCGAGAAGTAGAGGTTCGACCGCATCCCTATGAATTCTACCTGTACTTT
GATATCTAG

Upstream 100 bases:

>100_bases
TCAGCAGGACGCCGAAGGTGGCGTGCCACTGCTGTCTTATGCTGGGACGTAGTCGAGGCGCGCTGAAGGTGCCGTGAGCG
CGCGGAACAGGAGGAGAACC

Downstream 100 bases:

>100_bases
GGACGGGAACCGATGGTGGGTGGGGATGGGAGGTGCTCTGGTGGGGCCGTGAAAGGAGTACACTGATGCAGGGCCACGAG
GGCCACGACGCGGCTACCCG

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MYGSCTSPEHVLDLIRSRGIQIVDLRFTDLPGTWQHASLPASQLSLADFERGIGFDGSSIRGFQAIEESDMLLVPDPTTA
FIDPFAAVPTLVMICNVVDPETRQPYARDPRYVAQKAERYLRDLGIADTSFWGPEIEFFIFDHVSYEVLPYRMGFAIDSR
EGYWNSGRNDGTPNLGYTIRPKEGYFPAPPHDTFQDIRSEAALLLEQIGVPVEMHHHEVATAGQGEIDMRRDTLTAMADK
VMIYKYVIKNVARKHGCTATFMPKPLFGDNGNGMHTHQSLWRDGTNLFYAACHYAELSELALSYIAGLLTHVDALLALTS
PTTNSYKRLVPHYEAPVNVAFSKRNRSACIRIPMYTTGPENAAAKRVEFRPPDPSCNPYLAFAAMLMAGLDGIQRRLDAS
RFGPLDVNTYQLPPEQAAQVKSVPGSLGAALEALERDHDWLLEGNVFTRDLLETWVSYKREKELREVEVRPHPYEFYLYF
DI

Sequences:

>Translated_482_residues
MYGSCTSPEHVLDLIRSRGIQIVDLRFTDLPGTWQHASLPASQLSLADFERGIGFDGSSIRGFQAIEESDMLLVPDPTTA
FIDPFAAVPTLVMICNVVDPETRQPYARDPRYVAQKAERYLRDLGIADTSFWGPEIEFFIFDHVSYEVLPYRMGFAIDSR
EGYWNSGRNDGTPNLGYTIRPKEGYFPAPPHDTFQDIRSEAALLLEQIGVPVEMHHHEVATAGQGEIDMRRDTLTAMADK
VMIYKYVIKNVARKHGCTATFMPKPLFGDNGNGMHTHQSLWRDGTNLFYAACHYAELSELALSYIAGLLTHVDALLALTS
PTTNSYKRLVPHYEAPVNVAFSKRNRSACIRIPMYTTGPENAAAKRVEFRPPDPSCNPYLAFAAMLMAGLDGIQRRLDAS
RFGPLDVNTYQLPPEQAAQVKSVPGSLGAALEALERDHDWLLEGNVFTRDLLETWVSYKREKELREVEVRPHPYEFYLYF
DI
>Mature_482_residues
MYGSCTSPEHVLDLIRSRGIQIVDLRFTDLPGTWQHASLPASQLSLADFERGIGFDGSSIRGFQAIEESDMLLVPDPTTA
FIDPFAAVPTLVMICNVVDPETRQPYARDPRYVAQKAERYLRDLGIADTSFWGPEIEFFIFDHVSYEVLPYRMGFAIDSR
EGYWNSGRNDGTPNLGYTIRPKEGYFPAPPHDTFQDIRSEAALLLEQIGVPVEMHHHEVATAGQGEIDMRRDTLTAMADK
VMIYKYVIKNVARKHGCTATFMPKPLFGDNGNGMHTHQSLWRDGTNLFYAACHYAELSELALSYIAGLLTHVDALLALTS
PTTNSYKRLVPHYEAPVNVAFSKRNRSACIRIPMYTTGPENAAAKRVEFRPPDPSCNPYLAFAAMLMAGLDGIQRRLDAS
RFGPLDVNTYQLPPEQAAQVKSVPGSLGAALEALERDHDWLLEGNVFTRDLLETWVSYKREKELREVEVRPHPYEFYLYF
DI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=502, Percent_Identity=23.9043824701195, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1790301, Length=476, Percent_Identity=51.890756302521, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI87081870, Length=410, Percent_Identity=27.8048780487805, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 54311; Mature: 54311

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGSCTSPEHVLDLIRSRGIQIVDLRFTDLPGTWQHASLPASQLSLADFERGIGFDGSSI
CCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RGFQAIEESDMLLVPDPTTAFIDPFAAVPTLVMICNVVDPETRQPYARDPRYVAQKAERY
CCHHHHCCCCEEEECCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LRDLGIADTSFWGPEIEFFIFDHVSYEVLPYRMGFAIDSREGYWNSGRNDGTPNLGYTIR
HHHCCCCCCCCCCCCEEEEEEECCCEEEEEEEECEEEECCCCCCCCCCCCCCCCCCEEEC
PKEGYFPAPPHDTFQDIRSEAALLLEQIGVPVEMHHHEVATAGQGEIDMRRDTLTAMADK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHCCCCCCCCHHHHHHHHHHHHH
VMIYKYVIKNVARKHGCTATFMPKPLFGDNGNGMHTHQSLWRDGTNLFYAACHYAELSEL
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHH
ALSYIAGLLTHVDALLALTSPTTNSYKRLVPHYEAPVNVAFSKRNRSACIRIPMYTTGPE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEECCCC
NAAAKRVEFRPPDPSCNPYLAFAAMLMAGLDGIQRRLDASRFGPLDVNTYQLPPEQAAQV
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHH
KSVPGSLGAALEALERDHDWLLEGNVFTRDLLETWVSYKREKELREVEVRPHPYEFYLYF
HCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
DI
EC
>Mature Secondary Structure
MYGSCTSPEHVLDLIRSRGIQIVDLRFTDLPGTWQHASLPASQLSLADFERGIGFDGSSI
CCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RGFQAIEESDMLLVPDPTTAFIDPFAAVPTLVMICNVVDPETRQPYARDPRYVAQKAERY
CCHHHHCCCCEEEECCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LRDLGIADTSFWGPEIEFFIFDHVSYEVLPYRMGFAIDSREGYWNSGRNDGTPNLGYTIR
HHHCCCCCCCCCCCCEEEEEEECCCEEEEEEEECEEEECCCCCCCCCCCCCCCCCCEEEC
PKEGYFPAPPHDTFQDIRSEAALLLEQIGVPVEMHHHEVATAGQGEIDMRRDTLTAMADK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHCCCCCCCCHHHHHHHHHHHHH
VMIYKYVIKNVARKHGCTATFMPKPLFGDNGNGMHTHQSLWRDGTNLFYAACHYAELSEL
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHH
ALSYIAGLLTHVDALLALTSPTTNSYKRLVPHYEAPVNVAFSKRNRSACIRIPMYTTGPE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEEECCCC
NAAAKRVEFRPPDPSCNPYLAFAAMLMAGLDGIQRRLDASRFGPLDVNTYQLPPEQAAQV
CHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHH
KSVPGSLGAALEALERDHDWLLEGNVFTRDLLETWVSYKREKELREVEVRPHPYEFYLYF
HCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
DI
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099074 [H]