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The map label for this gene is narQ [C]

Identifier: 221633530

GI number: 221633530

Start: 1533718

End: 1535139

Strand: Reverse

Name: narQ [C]

Synonym: trd_1552

Alternate gene names: 221633530

Gene position: 1535139-1533718 (Counterclockwise)

Preceding gene: 221633533

Following gene: 221633529

Centisome position: 76.64

GC content: 63.78

Gene sequence:

>1422_bases
ATGACCGAGCGTTCGGAGCACGAGCTCGATCGGGCGATGGCCAGGCTGAAGCGGCTGGGCATCGTCCTGCCGCTCGCCTT
CTTGGCCGCACTCGTGGTAATCGGCCACGCGCTCGTTCCTGCGCTCGGTGCGAACTGGGCATGGATCGCCAGTGGAACGC
TGGCAGTCTTGGGGGTGGTCGTCTTTGCCGATTGGATCTTCCGCCCGATCGGTGAACTCTACCGCCGCCTCCTCATGCAA
CATGCGGAACTGGCTGCCCTGCACGAAGCTGGCGTGGCGATCACCGCTGATCTCGATCTCGCTCTGGTGTTGCAGCGAGT
GATCGACGAAGCTCGTCGGCTGGCCGGCGCGCGTTATGGCCTTTTGATCGTGCTGGATGATCAGGGCCAGCGTACGCTCT
TCACCTCGGGTTATCCGCGAACCGCCCGCTGCGAACAGAGCACGATGGGAGAGCACGGCATTTTGGAGGCAGTACTGCGG
GACGATCGCCCCCTCAACATCGAGGATCTCGATCGGTATCCCGGGACGCGGCACTATCCCGAGGGGCATCCCACCATGCG
CAGCTTGCTCGGGGTGCCCATCCGCTCGCAAGAGGGGGTGCTCGGTGGTCTCTATCTGGCCGATCGCGAGGATGGTCAGC
CATTCGATGAGCATGATCGCTCGCGGCTGGAGCGGTTCGCGACACAAGCTGCGCTCGCGATCGCAAATGCCCGGCTACAT
CGTCGCCTGATGAGTCTGGCGGTACACGAGGAGCGCGAGCGGATCGCCCGAGAAATGCACGACAGCCTCGCCCAGGTCTT
GGGGTATGTCATCACGAAGGTGTCGGCGTTGCGTGAACTCCTGCGCCAACCCGATCGCCTCGCAGAGGCAGAGCGTCATT
TGGAGCAACTCGAAAGAGCAGCGCGAGACGCCTACGCAGACGTTCGCGAAGCGATTCTCGGACTGCGGACCGGCCTCGCT
CCGAACCGGAGCCTCGTCGATGCGTTGCGGAGTTACGTGGAGCGGTGGCAGGACCAGAGCGGCGTCAACGCTCGACTCAT
CGTCGAACCCTCGGACGACAGCTTGCGCGGTCTCCAGCCGATGGCAGAACTCCAGCTGCTCCGCATCGTCCAGGAAGCAC
TGACGAATGTCCGCAAGCATGCGCGAGCGACCGAAGTCACGGTCCAGCTCGCTCGACTCGATGGAACGGTGTGCGTTCGT
GTGGCGGACAATGGGATCGGTTTCGATCCAGGAGCGCTGGAGCGCGGACAGTTCCCACGTTTTGGGCTCGCGACGATGCG
CGAGCGTGCCGAAGCGGTGGGAGGAAGGTTGCGGGTCACCTCGCTTCCGGGCCAGGGAACGACGGTCGAGGCGGTGCTTC
CGGTCGGCATCGTCCTGGCTGAAACGACTGGAGGACGAGATGCGTCTCTTGATCGTCGATGA

Upstream 100 bases:

>100_bases
GACGGCACGTCAGCGCTCGGCAATGTGGTCGATCGCGAACTGCGCGCCAGGACCTGCTATCCTCTGTATCGAGTAGGGTG
GCGACGGAGCGAGGGCGGCG

Downstream 100 bases:

>100_bases
CCATGCGCTCTTTCGCGATGGCCTGCGCAGCCTCCTGGAAGCACGGGGTGTCGAGGTCGTCGGCGAGGCGGCGAATGGAC
GCGAGGCGGTGGAACTCGCG

Product: putative two component system histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTERSEHELDRAMARLKRLGIVLPLAFLAALVVIGHALVPALGANWAWIASGTLAVLGVVVFADWIFRPIGELYRRLLMQ
HAELAALHEAGVAITADLDLALVLQRVIDEARRLAGARYGLLIVLDDQGQRTLFTSGYPRTARCEQSTMGEHGILEAVLR
DDRPLNIEDLDRYPGTRHYPEGHPTMRSLLGVPIRSQEGVLGGLYLADREDGQPFDEHDRSRLERFATQAALAIANARLH
RRLMSLAVHEERERIAREMHDSLAQVLGYVITKVSALRELLRQPDRLAEAERHLEQLERAARDAYADVREAILGLRTGLA
PNRSLVDALRSYVERWQDQSGVNARLIVEPSDDSLRGLQPMAELQLLRIVQEALTNVRKHARATEVTVQLARLDGTVCVR
VADNGIGFDPGALERGQFPRFGLATMRERAEAVGGRLRVTSLPGQGTTVEAVLPVGIVLAETTGGRDASLDRR

Sequences:

>Translated_473_residues
MTERSEHELDRAMARLKRLGIVLPLAFLAALVVIGHALVPALGANWAWIASGTLAVLGVVVFADWIFRPIGELYRRLLMQ
HAELAALHEAGVAITADLDLALVLQRVIDEARRLAGARYGLLIVLDDQGQRTLFTSGYPRTARCEQSTMGEHGILEAVLR
DDRPLNIEDLDRYPGTRHYPEGHPTMRSLLGVPIRSQEGVLGGLYLADREDGQPFDEHDRSRLERFATQAALAIANARLH
RRLMSLAVHEERERIAREMHDSLAQVLGYVITKVSALRELLRQPDRLAEAERHLEQLERAARDAYADVREAILGLRTGLA
PNRSLVDALRSYVERWQDQSGVNARLIVEPSDDSLRGLQPMAELQLLRIVQEALTNVRKHARATEVTVQLARLDGTVCVR
VADNGIGFDPGALERGQFPRFGLATMRERAEAVGGRLRVTSLPGQGTTVEAVLPVGIVLAETTGGRDASLDRR
>Mature_472_residues
TERSEHELDRAMARLKRLGIVLPLAFLAALVVIGHALVPALGANWAWIASGTLAVLGVVVFADWIFRPIGELYRRLLMQH
AELAALHEAGVAITADLDLALVLQRVIDEARRLAGARYGLLIVLDDQGQRTLFTSGYPRTARCEQSTMGEHGILEAVLRD
DRPLNIEDLDRYPGTRHYPEGHPTMRSLLGVPIRSQEGVLGGLYLADREDGQPFDEHDRSRLERFATQAALAIANARLHR
RLMSLAVHEERERIAREMHDSLAQVLGYVITKVSALRELLRQPDRLAEAERHLEQLERAARDAYADVREAILGLRTGLAP
NRSLVDALRSYVERWQDQSGVNARLIVEPSDDSLRGLQPMAELQLLRIVQEALTNVRKHARATEVTVQLARLDGTVCVRV
ADNGIGFDPGALERGQFPRFGLATMRERAEAVGGRLRVTSLPGQGTTVEAVLPVGIVLAETTGGRDASLDRR

Specific function: Acts As A Sensor For Nitrate/Nitrite And Transduces Signal Of Nitrate/Nitrite Availability To The Narl/Narp Proteins. Narq Probably Activates Narl And Narp By Phosphorylation. Narq Probably Negatively Regulates The Narl Protein By Dephosphorylation. [C]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=273, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787474, Length=250, Percent_Identity=32, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082311, Length=204, Percent_Identity=30.8823529411765, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 52374; Mature: 52243

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERSEHELDRAMARLKRLGIVLPLAFLAALVVIGHALVPALGANWAWIASGTLAVLGVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
VFADWIFRPIGELYRRLLMQHAELAALHEAGVAITADLDLALVLQRVIDEARRLAGARYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
LLIVLDDQGQRTLFTSGYPRTARCEQSTMGEHGILEAVLRDDRPLNIEDLDRYPGTRHYP
EEEEECCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCC
EGHPTMRSLLGVPIRSQEGVLGGLYLADREDGQPFDEHDRSRLERFATQAALAIANARLH
CCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRLMSLAVHEERERIAREMHDSLAQVLGYVITKVSALRELLRQPDRLAEAERHLEQLERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ARDAYADVREAILGLRTGLAPNRSLVDALRSYVERWQDQSGVNARLIVEPSDDSLRGLQP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCCH
MAELQLLRIVQEALTNVRKHARATEVTVQLARLDGTVCVRVADNGIGFDPGALERGQFPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCC
FGLATMRERAEAVGGRLRVTSLPGQGTTVEAVLPVGIVLAETTGGRDASLDRR
CHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TERSEHELDRAMARLKRLGIVLPLAFLAALVVIGHALVPALGANWAWIASGTLAVLGVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
VFADWIFRPIGELYRRLLMQHAELAALHEAGVAITADLDLALVLQRVIDEARRLAGARYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
LLIVLDDQGQRTLFTSGYPRTARCEQSTMGEHGILEAVLRDDRPLNIEDLDRYPGTRHYP
EEEEECCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCHHHHHCCCCCCCCC
EGHPTMRSLLGVPIRSQEGVLGGLYLADREDGQPFDEHDRSRLERFATQAALAIANARLH
CCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RRLMSLAVHEERERIAREMHDSLAQVLGYVITKVSALRELLRQPDRLAEAERHLEQLERA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ARDAYADVREAILGLRTGLAPNRSLVDALRSYVERWQDQSGVNARLIVEPSDDSLRGLQP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCCH
MAELQLLRIVQEALTNVRKHARATEVTVQLARLDGTVCVRVADNGIGFDPGALERGQFPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCC
FGLATMRERAEAVGGRLRVTSLPGQGTTVEAVLPVGIVLAETTGGRDASLDRR
CHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661927 [H]