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The map label for this gene is mhpE [C]

Identifier: 221633372

GI number: 221633372

Start: 1381393

End: 1382463

Strand: Reverse

Name: mhpE [C]

Synonym: trd_1392

Alternate gene names: 221633372

Gene position: 1382463-1381393 (Counterclockwise)

Preceding gene: 221633373

Following gene: 221633371

Centisome position: 69.02

GC content: 64.8

Gene sequence:

>1071_bases
ATGAAGACGCTCACGCCACCGCGCGTGACCGACACGACGCTGCGGGACGGTTCCCATGCCATGCGGCACCAGTTCACGCG
GGAGCAAGTGCGGGCCATCGTGGCTGCGCTCGACGAGGCTGGCGTGCCGGTCATCGAAGTCACCCATGGGGACGGACTAG
CGGGTAGCTCTATCCAGTACGGCTTTTCGGCGACGAGCGAACTCGAGCTGATCGAAGAAGCGGCGAAGACGGCCAAGCGA
GCCAAGATCGCGGCTCTCTTGCTCCCCGGAATCGGCACGCGCAGGGAGCTCTCGGCGGCGATCGAGCGGGGCATCAAGGT
CGTCCGCATCGCGACGCAGTGCACCGAGGCCGATATTTCCGAGCAGCACTTCGGGATGGCCAAAGAGATGGGCCTGGAAA
CGGTCGGCTTCCTCATGATGGCCCACATGCGCCCACCGGAGTTCCTGGCCGAGCAGGCAGCATTGATGGAGTCGTACGGT
GCCGACTGCGTCTACATCGTCGACTCCGCCGGCGCGATGTTGCCGGACGATGTCCGGCGCCGGGTCGCTGCGCTCAAGGA
GCGACTCTCCATTCAGGTCGGGTTCCATGCGCACAACAACCTCGGGTTGGCGATCGGGAATACACTGGCGGCCATCGAGG
AGGGAGCCGATCAGGTGGATGGCTGTTTGCGTGGGCTGGGTGCGGGTGCCGGCAACGCACCCACCGAGCTCATCGCGGCC
GTGCTCGACAAGTTGGGGATCAATCCGGGGCTCGATGTCTTCAAGTTGATGGATGCTGCCGAGTACATCGTGGCCCCGAT
CATGCCGTACCAGCCGATCCCGGATCGGGACGCGATCACGATCGGCTATGCTGGGGTCTATTCGACGTTCCTTCTCCACG
CTCGCCATATCGCAGCTGAGTTCGGGCTCGATCCGCGCGAAATCCTGGTCGAACTCGGTCGGCGCCAGGCGGTGGCGGGG
CAGGAGGACTGGATCCTGGATGTGGCGCTCGATATCCTGCGCCGCAAGCGCGCAGCCGAAGGAGCGGGCGATGCACGCGA
ACCAGCCGCAGCCGAGCGATCAGCTGATTGA

Upstream 100 bases:

>100_bases
CGCCCTATTCCGGCAATCTCGACATCATGACGGCAGCGGCCTGGCGGGTGGGTGAGTTGTTCGCCCAGCATTTGCTGGGT
ATCCGGCAGGAGGTGGCGGC

Downstream 100 bases:

>100_bases
CGAACCAGCCCCGATGATCGATCCCCGGCACTTCCGGCGGACGATGGGTCGGTTCGCGACGGGGGTGACGGTCATTACCG
TGGCGCGCCCGGAAGGAGCA

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKTLTPPRVTDTTLRDGSHAMRHQFTREQVRAIVAALDEAGVPVIEVTHGDGLAGSSIQYGFSATSELELIEEAAKTAKR
AKIAALLLPGIGTRRELSAAIERGIKVVRIATQCTEADISEQHFGMAKEMGLETVGFLMMAHMRPPEFLAEQAALMESYG
ADCVYIVDSAGAMLPDDVRRRVAALKERLSIQVGFHAHNNLGLAIGNTLAAIEEGADQVDGCLRGLGAGAGNAPTELIAA
VLDKLGINPGLDVFKLMDAAEYIVAPIMPYQPIPDRDAITIGYAGVYSTFLLHARHIAAEFGLDPREILVELGRRQAVAG
QEDWILDVALDILRRKRAAEGAGDAREPAAAERSAD

Sequences:

>Translated_356_residues
MKTLTPPRVTDTTLRDGSHAMRHQFTREQVRAIVAALDEAGVPVIEVTHGDGLAGSSIQYGFSATSELELIEEAAKTAKR
AKIAALLLPGIGTRRELSAAIERGIKVVRIATQCTEADISEQHFGMAKEMGLETVGFLMMAHMRPPEFLAEQAALMESYG
ADCVYIVDSAGAMLPDDVRRRVAALKERLSIQVGFHAHNNLGLAIGNTLAAIEEGADQVDGCLRGLGAGAGNAPTELIAA
VLDKLGINPGLDVFKLMDAAEYIVAPIMPYQPIPDRDAITIGYAGVYSTFLLHARHIAAEFGLDPREILVELGRRQAVAG
QEDWILDVALDILRRKRAAEGAGDAREPAAAERSAD
>Mature_356_residues
MKTLTPPRVTDTTLRDGSHAMRHQFTREQVRAIVAALDEAGVPVIEVTHGDGLAGSSIQYGFSATSELELIEEAAKTAKR
AKIAALLLPGIGTRRELSAAIERGIKVVRIATQCTEADISEQHFGMAKEMGLETVGFLMMAHMRPPEFLAEQAALMESYG
ADCVYIVDSAGAMLPDDVRRRVAALKERLSIQVGFHAHNNLGLAIGNTLAAIEEGADQVDGCLRGLGAGAGNAPTELIAA
VLDKLGINPGLDVFKLMDAAEYIVAPIMPYQPIPDRDAITIGYAGVYSTFLLHARHIAAEFGLDPREILVELGRRQAVAG
QEDWILDVALDILRRKRAAEGAGDAREPAAAERSAD

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=330, Percent_Identity=56.3636363636364, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1786261, Length=315, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_THERP (B9L2J0)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002522597.1
- ProteinModelPortal:   B9L2J0
- GeneID:   7354931
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_1392
- OMA:   ELGSDAQ
- ProtClustDB:   PRK08195
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 38125; Mature: 38125

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 169-169 BINDING 196-196 BINDING 287-287

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTPPRVTDTTLRDGSHAMRHQFTREQVRAIVAALDEAGVPVIEVTHGDGLAGSSIQY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEC
GFSATSELELIEEAAKTAKRAKIAALLLPGIGTRRELSAAIERGIKVVRIATQCTEADIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEHHHHHHHCCCC
EQHFGMAKEMGLETVGFLMMAHMRPPEFLAEQAALMESYGADCVYIVDSAGAMLPDDVRR
HHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
RVAALKERLSIQVGFHAHNNLGLAIGNTLAAIEEGADQVDGCLRGLGAGAGNAPTELIAA
HHHHHHHHHEEEEEEEECCCCCEEHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHH
VLDKLGINPGLDVFKLMDAAEYIVAPIMPYQPIPDRDAITIGYAGVYSTFLLHARHIAAE
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
FGLDPREILVELGRRQAVAGQEDWILDVALDILRRKRAAEGAGDAREPAAAERSAD
HCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTLTPPRVTDTTLRDGSHAMRHQFTREQVRAIVAALDEAGVPVIEVTHGDGLAGSSIQY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEC
GFSATSELELIEEAAKTAKRAKIAALLLPGIGTRRELSAAIERGIKVVRIATQCTEADIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEHHHHHHHCCCC
EQHFGMAKEMGLETVGFLMMAHMRPPEFLAEQAALMESYGADCVYIVDSAGAMLPDDVRR
HHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
RVAALKERLSIQVGFHAHNNLGLAIGNTLAAIEEGADQVDGCLRGLGAGAGNAPTELIAA
HHHHHHHHHEEEEEEEECCCCCEEHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHH
VLDKLGINPGLDVFKLMDAAEYIVAPIMPYQPIPDRDAITIGYAGVYSTFLLHARHIAAE
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
FGLDPREILVELGRRQAVAGQEDWILDVALDILRRKRAAEGAGDAREPAAAERSAD
HCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA