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The map label for this gene is ntaB [H]

Identifier: 221633371

GI number: 221633371

Start: 1380885

End: 1381379

Strand: Reverse

Name: ntaB [H]

Synonym: trd_1391

Alternate gene names: 221633371

Gene position: 1381379-1380885 (Counterclockwise)

Preceding gene: 221633372

Following gene: 221633370

Centisome position: 68.97

GC content: 66.46

Gene sequence:

>495_bases
ATGATCGATCCCCGGCACTTCCGGCGGACGATGGGTCGGTTCGCGACGGGGGTGACGGTCATTACCGTGGCGCGCCCGGA
AGGAGCACACGGGATGACGGCCAATGCCTTTCTGTCCGTCTCGCTCGATCCGCCGCTCGTCCTGGTTTCGGTCGATCGGC
GGGCACGGATGCACGCTTATTTGCTCGAAGCCACCCGCTATGGTGTGAGCGTGCTCGCCCGCGACCAGGAGCGGGCGGCG
CGGCATTTTGCCGGTAAGCCGCAAGCTGGGTACGAACCGGTCTTCGCCTGGCGGGAGGGAGTACCGCTCCTGGAGGGGGC
AGTCGCGCAGGTGGTTTGCGACGTGTGGGAGCGGGTGCCGGCGGGGGACCACACGCTGGTGTTGGGACGCGTGCGCTGGC
TGGACTATTGGGAGCGCGAGCCGCTCGTTTTCTTCGGTGGTGACTTCCGCTGCCTGGAGGTTCAGCTGCACGATACCAGC
ATGTGGTGGTGGTGA

Upstream 100 bases:

>100_bases
GCGCTCGATATCCTGCGCCGCAAGCGCGCAGCCGAAGGAGCGGGCGATGCACGCGAACCAGCCGCAGCCGAGCGATCAGC
TGATTGACGAACCAGCCCCG

Downstream 100 bases:

>100_bases
ACCGCGCGGCTGTGGAAGGGAGAGGGGACGTGACGATCGCCAAGTCGGTCGTGCACGCGGGACGATTGCCCACCCATTTT
CATCGGGCAGGAAGTGGAGA

Product: StyB protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MIDPRHFRRTMGRFATGVTVITVARPEGAHGMTANAFLSVSLDPPLVLVSVDRRARMHAYLLEATRYGVSVLARDQERAA
RHFAGKPQAGYEPVFAWREGVPLLEGAVAQVVCDVWERVPAGDHTLVLGRVRWLDYWEREPLVFFGGDFRCLEVQLHDTS
MWWW

Sequences:

>Translated_164_residues
MIDPRHFRRTMGRFATGVTVITVARPEGAHGMTANAFLSVSLDPPLVLVSVDRRARMHAYLLEATRYGVSVLARDQERAA
RHFAGKPQAGYEPVFAWREGVPLLEGAVAQVVCDVWERVPAGDHTLVLGRVRWLDYWEREPLVFFGGDFRCLEVQLHDTS
MWWW
>Mature_164_residues
MIDPRHFRRTMGRFATGVTVITVARPEGAHGMTANAFLSVSLDPPLVLVSVDRRARMHAYLLEATRYGVSVLARDQERAA
RHFAGKPQAGYEPVFAWREGVPLLEGAVAQVVCDVWERVPAGDHTLVLGRVRWLDYWEREPLVFFGGDFRCLEVQLHDTS
MWWW

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 18705; Mature: 18705

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPRHFRRTMGRFATGVTVITVARPEGAHGMTANAFLSVSLDPPLVLVSVDRRARMHAY
CCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHHH
LLEATRYGVSVLARDQERAARHFAGKPQAGYEPVFAWREGVPLLEGAVAQVVCDVWERVP
HHHHHHHCHHEEECCHHHHHHHCCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHHCC
AGDHTLVLGRVRWLDYWEREPLVFFGGDFRCLEVQLHDTSMWWW
CCCCEEEEEEEEEHHHCCCCCEEEECCCEEEEEEEEECCEECCC
>Mature Secondary Structure
MIDPRHFRRTMGRFATGVTVITVARPEGAHGMTANAFLSVSLDPPLVLVSVDRRARMHAY
CCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHHH
LLEATRYGVSVLARDQERAARHFAGKPQAGYEPVFAWREGVPLLEGAVAQVVCDVWERVP
HHHHHHHCHHEEECCHHHHHHHCCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHHCC
AGDHTLVLGRVRWLDYWEREPLVFFGGDFRCLEVQLHDTSMWWW
CCCCEEEEEEEEEHHHCCCCCEEEECCCEEEEEEEEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]