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The map label for this gene is hemD [H]

Identifier: 221633109

GI number: 221633109

Start: 1109560

End: 1111242

Strand: Reverse

Name: hemD [H]

Synonym: trd_1125

Alternate gene names: 221633109

Gene position: 1111242-1109560 (Counterclockwise)

Preceding gene: 221633111

Following gene: 221633108

Centisome position: 55.48

GC content: 64.17

Gene sequence:

>1683_bases
ATGGCAGCGCGGATTCGGATCGGATCGCGGGGGAGCACGCTGGCCCTCGCCCAGACCGAGGAGGTCGTCGAGCGGCTCCG
TCGCCTCGTTCCGGAGTGTTCAGTGACGGTCGTCCCCATCGTGACGACGGGTGATCGCGATCGAGCAACCTCGCTCACTG
TTCTCGGCGGAAGCGGCGTGTTCGCGAAGGAACTGCACGAAGCGCTCCTTGCGGGCACGATCGATCTCGCCGTTCACAGT
GCCAAAGACCTCCCCACTCAGCTTCCTCCTGGCCTGGTTCTCGTCGCGGTTTTGGCTCGAGAGGATGCTCGCGATGTGCT
GGTTACCTCGCCACCACGGAAGCTCGGGGAGTTGCCGGCGGGGGCGCGAGTAGGCACGAGTTCGCGGCGACGGCAAGCAC
TGCTCCAACGAGCACGGCCTGATCTCATCGTCGTCGATATTCGGGGCAATGTGGACACTCGCTTACGCAAACTGCGTGAA
GGGATTGTCGATGCCCTGGTGCTCGGAGCTGCCGGTCTGCGAAGACTCTCGCAGGCTGATCTCGTGGCCGAGTATCTGGA
TCCGGACGAGTTCGTTCCAGCACCGGGGCAGGGAGCTTTGGCAGTCGTCGCGCGTGAAAGCGATGGGCGACTCGAGCTTC
TCAGCCGACTCGACGAGCCGTTGGTCCACGAAGCGGTTGCGGTCGAACGAGCGTTCCTCGCAGCCTTCGGATCCGGCTGT
TCCGTGCCGCTCGGTGCCTACGCGACAATCGCAGGGAATCGATTGCGTTTTCGCATCGCTGTCCTTCTCCCCGATGGTCG
TTTCATCAGTCAGTGCCTGGATTGGCCTCGCGAGGAAGCGATCGAGCGTGCGCGGGAACTCGGGCTCACCCTGGCCACTC
GGGAAAAGCCCCATGTTTCCCAGCGAACAACCGATCCTCTCCGGGGCCGCCGCGTTCTTTTGGTACGCCCGGCTGGGCAA
GAAACGTTTCTCGTCGAGCGACTTCGTGCTCTCGGGGCTGAGGCAGTCATCCATCCGCTCATCCGCATCCTGCCGCCTGA
GGATTGGGGCCCTTTGGACGCTGCGCTGGCAGATCTGGCGCGTTTCGATTGGGTCGTCTTCACCAGCGCGAACGGGGTAC
GAGCGACGCTCGAGCGGTTGCGGCTTCTCGGCGGTGGATCGGAGCTGCTTCGCGAGAAACGACTCGCCGCCATCGGTCCG
GCGACTGCTCGTGCGTTGCGCGAATACGAGCTGGAGCCAGACATCGTCCCTGACAAATTCATCGCCGAGGCAGTTGCTGC
ACAGCTGATCGCGCGCGGCGTGGCAGGGGCGCGCGTTCTGGTCGCGCGTGCGGCAGAAGCACGCGAGATCCTGCCACGGC
GGCTCGAAGAAGCCGGGGCCACGGTCGTCGTTGTTCCGGCCTATCGGACGGAGCTGCTTCCACTCGACGAGGCGATACAG
GCGGCTCTCGAGGCAGGGACCATCGACTGGATCTTCTTGACTGCGAGTTCAACCGCGCGTGCGCTGGCTGCCGCACTCGG
TGACATCAAACGATTGTCCGATCGCATTCGTATCGCCGCGATCGGTCCGGTCACGGCCGGGACCGCTCGAGAGCTCGGAT
TACGCGTCGATGTCGTTGCCGATACCTACACGGCTGAGGGGTTGATCGAAGCGGTGGTGCGAGCGGAAAGGAGCAGGGGG
TGA

Upstream 100 bases:

>100_bases
TCGTGAGTAGAATCGCCGCGATGACATTGAGTGAGCAGATTCGATCAGATTTTATAGGAAATGGCTGACTCGGTTCGGGT
TGTAGGCGGGGGCGGATCGG

Downstream 100 bases:

>100_bases
GCATCGAGCGAGGGCGGTTTCGCCGGTTGCGTCGGCTGCGACAGACGGAGGGACTCCGTCGTCTGGTCCGCGAGACGCGT
TTGAACGTGGAGGACTTCAT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MAARIRIGSRGSTLALAQTEEVVERLRRLVPECSVTVVPIVTTGDRDRATSLTVLGGSGVFAKELHEALLAGTIDLAVHS
AKDLPTQLPPGLVLVAVLAREDARDVLVTSPPRKLGELPAGARVGTSSRRRQALLQRARPDLIVVDIRGNVDTRLRKLRE
GIVDALVLGAAGLRRLSQADLVAEYLDPDEFVPAPGQGALAVVARESDGRLELLSRLDEPLVHEAVAVERAFLAAFGSGC
SVPLGAYATIAGNRLRFRIAVLLPDGRFISQCLDWPREEAIERARELGLTLATREKPHVSQRTTDPLRGRRVLLVRPAGQ
ETFLVERLRALGAEAVIHPLIRILPPEDWGPLDAALADLARFDWVVFTSANGVRATLERLRLLGGGSELLREKRLAAIGP
ATARALREYELEPDIVPDKFIAEAVAAQLIARGVAGARVLVARAAEAREILPRRLEEAGATVVVVPAYRTELLPLDEAIQ
AALEAGTIDWIFLTASSTARALAAALGDIKRLSDRIRIAAIGPVTAGTARELGLRVDVVADTYTAEGLIEAVVRAERSRG

Sequences:

>Translated_560_residues
MAARIRIGSRGSTLALAQTEEVVERLRRLVPECSVTVVPIVTTGDRDRATSLTVLGGSGVFAKELHEALLAGTIDLAVHS
AKDLPTQLPPGLVLVAVLAREDARDVLVTSPPRKLGELPAGARVGTSSRRRQALLQRARPDLIVVDIRGNVDTRLRKLRE
GIVDALVLGAAGLRRLSQADLVAEYLDPDEFVPAPGQGALAVVARESDGRLELLSRLDEPLVHEAVAVERAFLAAFGSGC
SVPLGAYATIAGNRLRFRIAVLLPDGRFISQCLDWPREEAIERARELGLTLATREKPHVSQRTTDPLRGRRVLLVRPAGQ
ETFLVERLRALGAEAVIHPLIRILPPEDWGPLDAALADLARFDWVVFTSANGVRATLERLRLLGGGSELLREKRLAAIGP
ATARALREYELEPDIVPDKFIAEAVAAQLIARGVAGARVLVARAAEAREILPRRLEEAGATVVVVPAYRTELLPLDEAIQ
AALEAGTIDWIFLTASSTARALAAALGDIKRLSDRIRIAAIGPVTAGTARELGLRVDVVADTYTAEGLIEAVVRAERSRG
>Mature_559_residues
AARIRIGSRGSTLALAQTEEVVERLRRLVPECSVTVVPIVTTGDRDRATSLTVLGGSGVFAKELHEALLAGTIDLAVHSA
KDLPTQLPPGLVLVAVLAREDARDVLVTSPPRKLGELPAGARVGTSSRRRQALLQRARPDLIVVDIRGNVDTRLRKLREG
IVDALVLGAAGLRRLSQADLVAEYLDPDEFVPAPGQGALAVVARESDGRLELLSRLDEPLVHEAVAVERAFLAAFGSGCS
VPLGAYATIAGNRLRFRIAVLLPDGRFISQCLDWPREEAIERARELGLTLATREKPHVSQRTTDPLRGRRVLLVRPAGQE
TFLVERLRALGAEAVIHPLIRILPPEDWGPLDAALADLARFDWVVFTSANGVRATLERLRLLGGGSELLREKRLAAIGPA
TARALREYELEPDIVPDKFIAEAVAAQLIARGVAGARVLVARAAEAREILPRRLEEAGATVVVVPAYRTELLPLDEAIQA
ALEAGTIDWIFLTASSTARALAAALGDIKRLSDRIRIAAIGPVTAGTARELGLRVDVVADTYTAEGLIEAVVRAERSRG

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps [H]

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family [H]

Homologues:

Organism=Homo sapiens, GI66933009, Length=267, Percent_Identity=41.1985018726592, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI20149500, Length=267, Percent_Identity=41.1985018726592, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI48994974, Length=289, Percent_Identity=43.2525951557093, Blast_Score=195, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319996, Length=253, Percent_Identity=35.5731225296443, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI20130425, Length=258, Percent_Identity=41.4728682170543, Blast_Score=178, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418 [H]

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC [H]

EC number: =2.5.1.61 [H]

Molecular weight: Translated: 60177; Mature: 60046

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARIRIGSRGSTLALAQTEEVVERLRRLVPECSVTVVPIVTTGDRDRATSLTVLGGSGV
CCCEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEECCCCC
FAKELHEALLAGTIDLAVHSAKDLPTQLPPGLVLVAVLAREDARDVLVTSPPRKLGELPA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCHHHHCCCC
GARVGTSSRRRQALLQRARPDLIVVDIRGNVDTRLRKLREGIVDALVLGAAGLRRLSQAD
CCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAEYLDPDEFVPAPGQGALAVVARESDGRLELLSRLDEPLVHEAVAVERAFLAAFGSGC
HHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
SVPLGAYATIAGNRLRFRIAVLLPDGRFISQCLDWPREEAIERARELGLTLATREKPHVS
CCCCCCCEEECCCEEEEEEEEEECCCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCC
QRTTDPLRGRRVLLVRPAGQETFLVERLRALGAEAVIHPLIRILPPEDWGPLDAALADLA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RFDWVVFTSANGVRATLERLRLLGGGSELLREKRLAAIGPATARALREYELEPDIVPDKF
HHCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHH
IAEAVAAQLIARGVAGARVLVARAAEAREILPRRLEEAGATVVVVPAYRTELLPLDEAIQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHH
AALEAGTIDWIFLTASSTARALAAALGDIKRLSDRIRIAAIGPVTAGTARELGLRVDVVA
HHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCEEEEEEE
DTYTAEGLIEAVVRAERSRG
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AARIRIGSRGSTLALAQTEEVVERLRRLVPECSVTVVPIVTTGDRDRATSLTVLGGSGV
CCEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEECCCCC
FAKELHEALLAGTIDLAVHSAKDLPTQLPPGLVLVAVLAREDARDVLVTSPPRKLGELPA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCHHHHCCCC
GARVGTSSRRRQALLQRARPDLIVVDIRGNVDTRLRKLREGIVDALVLGAAGLRRLSQAD
CCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAEYLDPDEFVPAPGQGALAVVARESDGRLELLSRLDEPLVHEAVAVERAFLAAFGSGC
HHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
SVPLGAYATIAGNRLRFRIAVLLPDGRFISQCLDWPREEAIERARELGLTLATREKPHVS
CCCCCCCEEECCCEEEEEEEEEECCCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCC
QRTTDPLRGRRVLLVRPAGQETFLVERLRALGAEAVIHPLIRILPPEDWGPLDAALADLA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RFDWVVFTSANGVRATLERLRLLGGGSELLREKRLAAIGPATARALREYELEPDIVPDKF
HHCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHH
IAEAVAAQLIARGVAGARVLVARAAEAREILPRRLEEAGATVVVVPAYRTELLPLDEAIQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHH
AALEAGTIDWIFLTASSTARALAAALGDIKRLSDRIRIAAIGPVTAGTARELGLRVDVVA
HHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCEEEEEEE
DTYTAEGLIEAVVRAERSRG
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]