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The map label for this gene is nusA [H]

Identifier: 221632782

GI number: 221632782

Start: 762860

End: 764179

Strand: Reverse

Name: nusA [H]

Synonym: trd_0775

Alternate gene names: 221632782

Gene position: 764179-762860 (Counterclockwise)

Preceding gene: 221632791

Following gene: 221632781

Centisome position: 38.15

GC content: 62.42

Gene sequence:

>1320_bases
ATGAAGAGCGATCTCTACACCGCAATTGCACAGGTGGCTGCCGAGCGCGGCATTCCACGCGAGGCAGTCATGGAGTCCGT
CCAGCAAGCACTGCGGACAGTGTACAAGAAGGCGACGGGCTCCGACGAGGAAGTCGAAATCGAAATCGACGTCAACACCG
GAAAGATCCGTATCTTCGCGCCGAAGCGGGTCGTCGAGAAGGTAAACGATCCGCTCAGCGAGATTTCCATCGAGGAGGCG
CGCAAGATCGATCCCCGTGCGATCGTCGGGGACATCGTCCGGATCGAGCGCCAGCCGTCCAACTTCGGCCGGATCGCGGC
CCAGACCGCCAAGCAGGTGATCCTGCAGCGGATCCGGGATTTCGAGCGCGACACGATCTACAACGAATTCATCGATCGCG
TCGGCGAAGTCCTGACTGGTACGGTGCAGCGCGTCGATGCGCGTGCGGTAATCCTGAGCTTCGGCAAAGCGGAGGCTATT
CTCCCGGCACGGGAGCAGATTCCCGGTGAGCGGTATCGCCCAGGGCAGCGGCTCAAGGTGTACCTGGTCGATGTCACCAA
GGACGCGAAGGGACCGCAGCTTTTGGTGTCCCGCACGCACCCGAACTTGATCAAGCGACTGCTCGAGCTCGAGGTACCGG
AGGTTGCGAGCGGTGCCGTCGAAGTCATGGCCATCGCCCGTGAACCAGGCCAGCGATCCAAAGTCGCTGTCGCTGCTCGG
CAGGACAAGGTCGACCCGGTCGGCGCCTGCGTCGGTGTCCGCGGCGTGCGGATCCAGAGCATCGTCACCGAGCTTTCGGG
TGAGAAGATCGACGTGATCGAGTGGTCGTCGGACATCCGCACGTTCATCGCCAACGCCCTGAGCCCGGCCAAGCCGATCA
CGGTCATCCTCAACGAGGATGAGAAGGTCGCTCGGGTCGTCGTGCCGCCGGAGCAGATGTCGCAGGCGATCGGCAAGGAC
GGCCAAAACGCCCGGCTGGCGTACAAGCTGACCGGTTGGCGGATCGATATCAAGGATCCAGAGTCGCTGAAGGGGGAGGA
GGCACTGCTCGTTCGCTCGGCCACGAGCTTGCCCGAGCTTCCGCCCGATCTCATGGCACTCGGTCGGCAGCCACGCCTCG
TTCGCCCGGACGGCACGTTCAGCATCCGGGAGCGCGAGTTCGGTCCGCTGCCCAGCGATCTCGTGCTCAAGAGCGTCGAC
GTGGAAATCGTCAACGGCGTCGTCAATGTCTATTACGATCGCGAACTTCGTGCTCGGTACGATTTCGAAACCGGTCGTGA
GCTACCATTGACCGATGACGAAACGGCGAATGTCCGTTGA

Upstream 100 bases:

>100_bases
GAGCGCCCCGGACAAGTGGGCAATGCCCACTTTAATTTTTGCCCTGGCTGGCAGCCCACCAGCCGGATTCCGGAGCGCAT
GACGAAGGGGGGCGATCACG

Downstream 100 bases:

>100_bases
TCCCGTTTCTCGTCGAGGAGGTCGTGACGATGAGCGAGCGTCCCGACCAGCGCAAGCGCCGCGGACCGCGGCTCAAGCAT
TTGCCTGTCCGCACCTGCGT

Product: transcription termination factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKSDLYTAIAQVAAERGIPREAVMESVQQALRTVYKKATGSDEEVEIEIDVNTGKIRIFAPKRVVEKVNDPLSEISIEEA
RKIDPRAIVGDIVRIERQPSNFGRIAAQTAKQVILQRIRDFERDTIYNEFIDRVGEVLTGTVQRVDARAVILSFGKAEAI
LPAREQIPGERYRPGQRLKVYLVDVTKDAKGPQLLVSRTHPNLIKRLLELEVPEVASGAVEVMAIAREPGQRSKVAVAAR
QDKVDPVGACVGVRGVRIQSIVTELSGEKIDVIEWSSDIRTFIANALSPAKPITVILNEDEKVARVVVPPEQMSQAIGKD
GQNARLAYKLTGWRIDIKDPESLKGEEALLVRSATSLPELPPDLMALGRQPRLVRPDGTFSIREREFGPLPSDLVLKSVD
VEIVNGVVNVYYDRELRARYDFETGRELPLTDDETANVR

Sequences:

>Translated_439_residues
MKSDLYTAIAQVAAERGIPREAVMESVQQALRTVYKKATGSDEEVEIEIDVNTGKIRIFAPKRVVEKVNDPLSEISIEEA
RKIDPRAIVGDIVRIERQPSNFGRIAAQTAKQVILQRIRDFERDTIYNEFIDRVGEVLTGTVQRVDARAVILSFGKAEAI
LPAREQIPGERYRPGQRLKVYLVDVTKDAKGPQLLVSRTHPNLIKRLLELEVPEVASGAVEVMAIAREPGQRSKVAVAAR
QDKVDPVGACVGVRGVRIQSIVTELSGEKIDVIEWSSDIRTFIANALSPAKPITVILNEDEKVARVVVPPEQMSQAIGKD
GQNARLAYKLTGWRIDIKDPESLKGEEALLVRSATSLPELPPDLMALGRQPRLVRPDGTFSIREREFGPLPSDLVLKSVD
VEIVNGVVNVYYDRELRARYDFETGRELPLTDDETANVR
>Mature_439_residues
MKSDLYTAIAQVAAERGIPREAVMESVQQALRTVYKKATGSDEEVEIEIDVNTGKIRIFAPKRVVEKVNDPLSEISIEEA
RKIDPRAIVGDIVRIERQPSNFGRIAAQTAKQVILQRIRDFERDTIYNEFIDRVGEVLTGTVQRVDARAVILSFGKAEAI
LPAREQIPGERYRPGQRLKVYLVDVTKDAKGPQLLVSRTHPNLIKRLLELEVPEVASGAVEVMAIAREPGQRSKVAVAAR
QDKVDPVGACVGVRGVRIQSIVTELSGEKIDVIEWSSDIRTFIANALSPAKPITVILNEDEKVARVVVPPEQMSQAIGKD
GQNARLAYKLTGWRIDIKDPESLKGEEALLVRSATSLPELPPDLMALGRQPRLVRPDGTFSIREREFGPLPSDLVLKSVD
VEIVNGVVNVYYDRELRARYDFETGRELPLTDDETANVR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=348, Percent_Identity=36.7816091954023, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 48820; Mature: 48820

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDLYTAIAQVAAERGIPREAVMESVQQALRTVYKKATGSDEEVEIEIDVNTGKIRIFA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEE
PKRVVEKVNDPLSEISIEEARKIDPRAIVGDIVRIERQPSNFGRIAAQTAKQVILQRIRD
HHHHHHHHCCCHHHCCHHHHHCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHH
FERDTIYNEFIDRVGEVLTGTVQRVDARAVILSFGKAEAILPAREQIPGERYRPGQRLKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCCCCCCCCEEE
YLVDVTKDAKGPQLLVSRTHPNLIKRLLELEVPEVASGAVEVMAIAREPGQRSKVAVAAR
EEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHCCCEEEEEECCCCCCCCEEEEEEC
QDKVDPVGACVGVRGVRIQSIVTELSGEKIDVIEWSSDIRTFIANALSPAKPITVILNED
CCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEECCC
EKVARVVVPPEQMSQAIGKDGQNARLAYKLTGWRIDIKDPESLKGEEALLVRSATSLPEL
CCEEEEEECHHHHHHHHCCCCCCCEEEEEEECEEEECCCCCCCCCCCEEEEECCCCCCCC
PPDLMALGRQPRLVRPDGTFSIREREFGPLPSDLVLKSVDVEIVNGVVNVYYDRELRARY
CHHHHHCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHEEECCHHHEEE
DFETGRELPLTDDETANVR
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSDLYTAIAQVAAERGIPREAVMESVQQALRTVYKKATGSDEEVEIEIDVNTGKIRIFA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEE
PKRVVEKVNDPLSEISIEEARKIDPRAIVGDIVRIERQPSNFGRIAAQTAKQVILQRIRD
HHHHHHHHCCCHHHCCHHHHHCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHH
FERDTIYNEFIDRVGEVLTGTVQRVDARAVILSFGKAEAILPAREQIPGERYRPGQRLKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHCCCCCCCCCCCEEE
YLVDVTKDAKGPQLLVSRTHPNLIKRLLELEVPEVASGAVEVMAIAREPGQRSKVAVAAR
EEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHCCCEEEEEECCCCCCCCEEEEEEC
QDKVDPVGACVGVRGVRIQSIVTELSGEKIDVIEWSSDIRTFIANALSPAKPITVILNED
CCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEECCC
EKVARVVVPPEQMSQAIGKDGQNARLAYKLTGWRIDIKDPESLKGEEALLVRSATSLPEL
CCEEEEEECHHHHHHHHCCCCCCCEEEEEEECEEEECCCCCCCCCCCEEEEECCCCCCCC
PPDLMALGRQPRLVRPDGTFSIREREFGPLPSDLVLKSVDVEIVNGVVNVYYDRELRARY
CHHHHHCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHEEECCHHHEEE
DFETGRELPLTDDETANVR
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]