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The map label for this gene is purA

Identifier: 221632679

GI number: 221632679

Start: 656893

End: 658188

Strand: Reverse

Name: purA

Synonym: trd_0662

Alternate gene names: 221632679

Gene position: 658188-656893 (Counterclockwise)

Preceding gene: 221632680

Following gene: 221632678

Centisome position: 32.86

GC content: 62.81

Gene sequence:

>1296_bases
ATGCCAGTCACGATCGTTCTCGGCGGCCAGTGGGGCGACGAGGGGAAAGGGAAAATCACCGATGCCCTCGCCGCCACGGC
CGATATCGTCGTCCGGCCGAACGGGTCCACCAACGCTGGTCACACCGTCGTCACCGATGAAGGTGTCTTCAAGCTCCACG
TGATCCCCTCGGGGATCCTTTACCCGCACTGCACCTGCATCATCGGTGCCGGGGTTGCCGTCTCGCCACCTGATTTCCTT
CGCGAGATCGAAAGCTTGCGCGAGCGACACCCGCGTCTCGGGCAGCTCTACGTCAGCGACCGAGCCCATGTCATCATGCC
GTATCACCCCCTCCTCGATCTCTACGAAGAACGGCGACGGGGAGCTGCCGGCATCGGCACGACCCTGCGCGGAAATGGCC
CAGCCTTCACCGACAAGATCGCTCGCCGGGGCATCCGCATCGCCGACCTTTTGCCTGGATCCGAGCGCTCGCTGCGTCAT
AAACTCGAGACGCTTTTGCCCGAGAAGAACACACTGTTCATCCATCTTTACGGCGAGTCACCGCTCGACCTCGATCAGCT
CTTCGAGCAAGCGCGTACCTGGGGCGAGGCACTCGCTCCGTACGTGATCGCGGCGGAAGTCTTCGTACAAGACGCACTCG
ATGCTGGAAAGTCAGTGATCATCGAAGCCGCACAAGGCACCATGCTGGACCCAGACTATGGAACGTACCCCTATGTCACG
TCCAGTTCCCCCACCGCAGCCGGCGCCTGCCAGGGAGCTGGGGTCGCACCGACGCAAGTCGATCGAATCGTCGGTGTCTT
CAAAGCCTACACGACGCGGGTCGGCGCTGGTCCCTTCCCAACCGAACTCACTGACGAGACTGGCCAGCTCATTCGGGAAC
GCGGCCGCGAGTACGGCACGACGACCGGGCGACCGCGGCGAGTCGGTTGGTTCGATGCGGTAGCCGCGCGGTATTCTGCA
CGTCTCAACGGCATGACCGAAGCGGCACTCACGAAACTCGACATGCTCGACCCCTTGCCGGAAGTGCGCATCTGCGTCGG
CTATCGTATCGACAACCAGGAAGTCTCGGCGCCGCCTGCCCAAGTGGAGCGCTACGCAGCGGTGGAGCCGGTCTATGAGG
TTTTGCCCGGCTGGCAGTGCGATACCAGTCAGGTAACCTCCTTCGATGATCTGCCGCCGGAAGCACAGCGCTACGTGGCT
CGGATCGAGGAACTCATCGGTGTACCCATCACGATGATCGGCGTCGGACCAGCCCGTCGTCAGATTCTCTGGCGACCAGC
CGGTGCCCTGACGTGA

Upstream 100 bases:

>100_bases
AACTCCGTCAGGTTCGCACGCGCGCCTGATCGCCCTGGCGATGCTATAGTTCACCGGGCAGCATCGCCCGGAGCGACCGA
GCTGACGAGGAGTCTACGCG

Downstream 100 bases:

>100_bases
TCGAGACCGAGGAGACCAGCATGTTGGAAGCCTGGCAAATTCAGGAGATCATTCCTCACCGCTATCCCTTTCTCCTGGTC
GATCGCATCATCGAGATCGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPVTIVLGGQWGDEGKGKITDALAATADIVVRPNGSTNAGHTVVTDEGVFKLHVIPSGILYPHCTCIIGAGVAVSPPDFL
REIESLRERHPRLGQLYVSDRAHVIMPYHPLLDLYEERRRGAAGIGTTLRGNGPAFTDKIARRGIRIADLLPGSERSLRH
KLETLLPEKNTLFIHLYGESPLDLDQLFEQARTWGEALAPYVIAAEVFVQDALDAGKSVIIEAAQGTMLDPDYGTYPYVT
SSSPTAAGACQGAGVAPTQVDRIVGVFKAYTTRVGAGPFPTELTDETGQLIRERGREYGTTTGRPRRVGWFDAVAARYSA
RLNGMTEAALTKLDMLDPLPEVRICVGYRIDNQEVSAPPAQVERYAAVEPVYEVLPGWQCDTSQVTSFDDLPPEAQRYVA
RIEELIGVPITMIGVGPARRQILWRPAGALT

Sequences:

>Translated_431_residues
MPVTIVLGGQWGDEGKGKITDALAATADIVVRPNGSTNAGHTVVTDEGVFKLHVIPSGILYPHCTCIIGAGVAVSPPDFL
REIESLRERHPRLGQLYVSDRAHVIMPYHPLLDLYEERRRGAAGIGTTLRGNGPAFTDKIARRGIRIADLLPGSERSLRH
KLETLLPEKNTLFIHLYGESPLDLDQLFEQARTWGEALAPYVIAAEVFVQDALDAGKSVIIEAAQGTMLDPDYGTYPYVT
SSSPTAAGACQGAGVAPTQVDRIVGVFKAYTTRVGAGPFPTELTDETGQLIRERGREYGTTTGRPRRVGWFDAVAARYSA
RLNGMTEAALTKLDMLDPLPEVRICVGYRIDNQEVSAPPAQVERYAAVEPVYEVLPGWQCDTSQVTSFDDLPPEAQRYVA
RIEELIGVPITMIGVGPARRQILWRPAGALT
>Mature_430_residues
PVTIVLGGQWGDEGKGKITDALAATADIVVRPNGSTNAGHTVVTDEGVFKLHVIPSGILYPHCTCIIGAGVAVSPPDFLR
EIESLRERHPRLGQLYVSDRAHVIMPYHPLLDLYEERRRGAAGIGTTLRGNGPAFTDKIARRGIRIADLLPGSERSLRHK
LETLLPEKNTLFIHLYGESPLDLDQLFEQARTWGEALAPYVIAAEVFVQDALDAGKSVIIEAAQGTMLDPDYGTYPYVTS
SSPTAAGACQGAGVAPTQVDRIVGVFKAYTTRVGAGPFPTELTDETGQLIRERGREYGTTTGRPRRVGWFDAVAARYSAR
LNGMTEAALTKLDMLDPLPEVRICVGYRIDNQEVSAPPAQVERYAAVEPVYEVLPGWQCDTSQVTSFDDLPPEAQRYVAR
IEELIGVPITMIGVGPARRQILWRPAGALT

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=41.2587412587413, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=40.9836065573771, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=38.9873417721519, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1790620, Length=421, Percent_Identity=45.6057007125891, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=42.093023255814, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=42.093023255814, Blast_Score=336, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=39.7228637413395, Blast_Score=353, Evalue=3e-98,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=40.2352941176471, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_THERP (B9KYV8)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002521900.1
- GeneID:   7354815
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_0662
- OMA:   DYVVRYQ
- ProtClustDB:   CLSK2807623
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46852; Mature: 46721

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTIVLGGQWGDEGKGKITDALAATADIVVRPNGSTNAGHTVVTDEGVFKLHVIPSGIL
CCEEEEECCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCC
YPHCTCIIGAGVAVSPPDFLREIESLRERHPRLGQLYVSDRAHVIMPYHPLLDLYEERRR
HHCHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCHHHHHHHHHHCC
GAAGIGTTLRGNGPAFTDKIARRGIRIADLLPGSERSLRHKLETLLPEKNTLFIHLYGES
CCCCCCCEECCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCC
PLDLDQLFEQARTWGEALAPYVIAAEVFVQDALDAGKSVIIEAAQGTMLDPDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCEEE
SSSPTAAGACQGAGVAPTQVDRIVGVFKAYTTRVGAGPFPTELTDETGQLIRERGREYGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHCCC
TTGRPRRVGWFDAVAARYSARLNGMTEAALTKLDMLDPLPEVRICVGYRIDNQEVSAPPA
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHH
QVERYAAVEPVYEVLPGWQCDTSQVTSFDDLPPEAQRYVARIEELIGVPITMIGVGPARR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHH
QILWRPAGALT
HEEECCCCCCC
>Mature Secondary Structure 
PVTIVLGGQWGDEGKGKITDALAATADIVVRPNGSTNAGHTVVTDEGVFKLHVIPSGIL
CEEEEECCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCC
YPHCTCIIGAGVAVSPPDFLREIESLRERHPRLGQLYVSDRAHVIMPYHPLLDLYEERRR
HHCHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCHHHHHHHHHHCC
GAAGIGTTLRGNGPAFTDKIARRGIRIADLLPGSERSLRHKLETLLPEKNTLFIHLYGES
CCCCCCCEECCCCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCC
PLDLDQLFEQARTWGEALAPYVIAAEVFVQDALDAGKSVIIEAAQGTMLDPDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCEEE
SSSPTAAGACQGAGVAPTQVDRIVGVFKAYTTRVGAGPFPTELTDETGQLIRERGREYGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHCCC
TTGRPRRVGWFDAVAARYSARLNGMTEAALTKLDMLDPLPEVRICVGYRIDNQEVSAPPA
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHH
QVERYAAVEPVYEVLPGWQCDTSQVTSFDDLPPEAQRYVARIEELIGVPITMIGVGPARR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHH
QILWRPAGALT
HEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA