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The map label for this gene is amyA [C]

Identifier: 220932709

GI number: 220932709

Start: 2001024

End: 2002352

Strand: Reverse

Name: amyA [C]

Synonym: Hore_18730

Alternate gene names: 220932709

Gene position: 2002352-2001024 (Counterclockwise)

Preceding gene: 220932710

Following gene: 220932708

Centisome position: 77.67

GC content: 38.37

Gene sequence:

>1329_bases
ATGAAGGTTTTTAGAAGGCGTCTTTTCATTGTTTTGTTCAGTCTGCTTCTGTTGATTTCAGTTATTACTTCTGCAAGGGC
CGGGGTGTTGATGCAAGGGTTTTACTGGGATACTCCATATCAGGGAGAGTGGTATGACCATATAGCCTCCAAAGCTGAAG
AACTTTCAAATGCTGGATTTACTGCAATATGGTTTCCTTCGCCATGTAAAGGTGATAGTGGCGGTTATTCCATGGGTTAT
GACGTTTTTGATCATTATGACCTGGGGAATTATTACCAGCAGGGGACTACTGAAACCCGATTTGGGAGTAAAAATGAACT
GTTAAATGCAATCAATGCTTACCACAGTGAAGGAATGCAGGTGTATGTTGATACTGTCATGAACCACATGATGGGTGGTG
AACAGGAATGGAATCCGAATACAAATTCGTATACATATACCAGGTTTGATTACCCCCACGATACTTTTGAAAAGAATTAT
AAACATTTTCATCCAAATTATACCCACCCAGATAATGACCCCCCTTATCATAGTAAAGAATTTGGCGAAGATGTCTGTTA
TTATAATGACTATAACTATATGGGGAATGGGTTAAAAAATTGGGCAGCCTGGTTAAAGAATAATATTGGATTTGATGGGT
ATAGATTAGATTTTGTTAAAGGTATAGAACCTGATTATATTAAATCCTGGAAACAAACTTCTCCAATGAGTAGTAGTTTT
GTCGTGGGTGAATACTGGGATGGTAACAGGGATACCCTGGATTGGTGGGCAAATTATACTGGTTGTCATGTTTTTGACTT
TGCATTATTTTACACATTAAAAGATATGTGTAATAGCGACGGCTACTATGATATGAGAGGGCTACAGGATGCAGGGTTGG
TGGAAATAAACCCTTACAGGGCGGTAACATTTGTAGAAAACCATGATACAGATGAACATGACCCGGTAACAAAAAATAAA
TTAATGGCCTATGCTTATATTTTAACCCATGAGGGTTATCCTACAGTATTTTGGAAAGATTATTATGTATATGATTTAAA
GGATGAAATAAATAACCTGGTCTGGATACATGAGAACCTGGCCTCAGGAACTACCAGTAATCTTTACGCTGATGATAGTT
TGTATATTGCCCAACGAAATGGTAATCCCGGACTTGTGGTCGGGCTCAACGATAGTTCCAGTTGGAAGAGTAAATGGGTT
CAAACTAAATGGAGTAATGTTACTTTACATGACTATACCGGACAGGCCGGAGATGTATATGTGGATAGTAACGGCTGGGT
AGAAATTTCAATACCACCAAAAGGATATAGTGTCTACTCTCCATATTAA

Upstream 100 bases:

>100_bases
TAATCTAAATAACAATTATTTTAACATAAAACTAATGATATATCCATTGATATATTTTTTATACGTCGTAGACTAATATA
CTATTAAGGGGGCCGGGTTA

Downstream 100 bases:

>100_bases
TGAGACTTTAAAAGAACCCGGGGTTTCCCGGGTTCTTTATTTTTTAAATTTTGGTTTAAGAAGGATTTTTTTAAAATATA
TAGAATATTAATTATAAAAC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKVFRRRLFIVLFSLLLLISVITSARAGVLMQGFYWDTPYQGEWYDHIASKAEELSNAGFTAIWFPSPCKGDSGGYSMGY
DVFDHYDLGNYYQQGTTETRFGSKNELLNAINAYHSEGMQVYVDTVMNHMMGGEQEWNPNTNSYTYTRFDYPHDTFEKNY
KHFHPNYTHPDNDPPYHSKEFGEDVCYYNDYNYMGNGLKNWAAWLKNNIGFDGYRLDFVKGIEPDYIKSWKQTSPMSSSF
VVGEYWDGNRDTLDWWANYTGCHVFDFALFYTLKDMCNSDGYYDMRGLQDAGLVEINPYRAVTFVENHDTDEHDPVTKNK
LMAYAYILTHEGYPTVFWKDYYVYDLKDEINNLVWIHENLASGTTSNLYADDSLYIAQRNGNPGLVVGLNDSSSWKSKWV
QTKWSNVTLHDYTGQAGDVYVDSNGWVEISIPPKGYSVYSPY

Sequences:

>Translated_442_residues
MKVFRRRLFIVLFSLLLLISVITSARAGVLMQGFYWDTPYQGEWYDHIASKAEELSNAGFTAIWFPSPCKGDSGGYSMGY
DVFDHYDLGNYYQQGTTETRFGSKNELLNAINAYHSEGMQVYVDTVMNHMMGGEQEWNPNTNSYTYTRFDYPHDTFEKNY
KHFHPNYTHPDNDPPYHSKEFGEDVCYYNDYNYMGNGLKNWAAWLKNNIGFDGYRLDFVKGIEPDYIKSWKQTSPMSSSF
VVGEYWDGNRDTLDWWANYTGCHVFDFALFYTLKDMCNSDGYYDMRGLQDAGLVEINPYRAVTFVENHDTDEHDPVTKNK
LMAYAYILTHEGYPTVFWKDYYVYDLKDEINNLVWIHENLASGTTSNLYADDSLYIAQRNGNPGLVVGLNDSSSWKSKWV
QTKWSNVTLHDYTGQAGDVYVDSNGWVEISIPPKGYSVYSPY
>Mature_442_residues
MKVFRRRLFIVLFSLLLLISVITSARAGVLMQGFYWDTPYQGEWYDHIASKAEELSNAGFTAIWFPSPCKGDSGGYSMGY
DVFDHYDLGNYYQQGTTETRFGSKNELLNAINAYHSEGMQVYVDTVMNHMMGGEQEWNPNTNSYTYTRFDYPHDTFEKNY
KHFHPNYTHPDNDPPYHSKEFGEDVCYYNDYNYMGNGLKNWAAWLKNNIGFDGYRLDFVKGIEPDYIKSWKQTSPMSSSF
VVGEYWDGNRDTLDWWANYTGCHVFDFALFYTLKDMCNSDGYYDMRGLQDAGLVEINPYRAVTFVENHDTDEHDPVTKNK
LMAYAYILTHEGYPTVFWKDYYVYDLKDEINNLVWIHENLASGTTSNLYADDSLYIAQRNGNPGLVVGLNDSSSWKSKWV
QTKWSNVTLHDYTGQAGDVYVDSNGWVEISIPPKGYSVYSPY

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=477, Percent_Identity=27.2536687631027, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 51261; Mature: 51261

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFRRRLFIVLFSLLLLISVITSARAGVLMQGFYWDTPYQGEWYDHIASKAEELSNAGF
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECEECCCCCCCCHHHHHHHHHHHHHCCCE
TAIWFPSPCKGDSGGYSMGYDVFDHYDLGNYYQQGTTETRFGSKNELLNAINAYHSEGMQ
EEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCE
VYVDTVMNHMMGGEQEWNPNTNSYTYTRFDYPHDTFEKNYKHFHPNYTHPDNDPPYHSKE
EEHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCHH
FGEDVCYYNDYNYMGNGLKNWAAWLKNNIGFDGYRLDFVKGIEPDYIKSWKQTSPMSSSF
HCCCCEEECCCHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCE
VVGEYWDGNRDTLDWWANYTGCHVFDFALFYTLKDMCNSDGYYDMRGLQDAGLVEINPYR
EEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEECCCE
AVTFVENHDTDEHDPVTKNKLMAYAYILTHEGYPTVFWKDYYVYDLKDEINNLVWIHENL
EEEEEECCCCCCCCCCCHHHHEEEEEEEECCCCCEEEEECEEEEECHHCCCCEEEEEECC
ASGTTSNLYADDSLYIAQRNGNPGLVVGLNDSSSWKSKWVQTKWSNVTLHDYTGQAGDVY
CCCCCCCEEECCEEEEEEECCCCCEEEEECCCCHHHHHHHEECCCCEEEEECCCCCCCEE
VDSNGWVEISIPPKGYSVYSPY
ECCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKVFRRRLFIVLFSLLLLISVITSARAGVLMQGFYWDTPYQGEWYDHIASKAEELSNAGF
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECEECCCCCCCCHHHHHHHHHHHHHCCCE
TAIWFPSPCKGDSGGYSMGYDVFDHYDLGNYYQQGTTETRFGSKNELLNAINAYHSEGMQ
EEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCE
VYVDTVMNHMMGGEQEWNPNTNSYTYTRFDYPHDTFEKNYKHFHPNYTHPDNDPPYHSKE
EEHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCHH
FGEDVCYYNDYNYMGNGLKNWAAWLKNNIGFDGYRLDFVKGIEPDYIKSWKQTSPMSSSF
HCCCCEEECCCHHHCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCE
VVGEYWDGNRDTLDWWANYTGCHVFDFALFYTLKDMCNSDGYYDMRGLQDAGLVEINPYR
EEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEECCCE
AVTFVENHDTDEHDPVTKNKLMAYAYILTHEGYPTVFWKDYYVYDLKDEINNLVWIHENL
EEEEEECCCCCCCCCCCHHHHEEEEEEEECCCCCEEEEECEEEEECHHCCCCEEEEEECC
ASGTTSNLYADDSLYIAQRNGNPGLVVGLNDSSSWKSKWVQTKWSNVTLHDYTGQAGDVY
CCCCCCCEEECCEEEEEEECCCCCEEEEECCCCHHHHHHHEECCCCEEEEECCCCCCCEE
VDSNGWVEISIPPKGYSVYSPY
ECCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6185474; 6170539; 2830166; 6156671; 10924103 [H]