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The map label for this gene is hisS

Identifier: 220932052

GI number: 220932052

Start: 1307761

End: 1309020

Strand: Reverse

Name: hisS

Synonym: Hore_12150

Alternate gene names: 220932052

Gene position: 1309020-1307761 (Counterclockwise)

Preceding gene: 220932053

Following gene: 220932051

Centisome position: 50.77

GC content: 38.81

Gene sequence:

>1260_bases
ATGGATGTAAAGGCACCACGGGGGACAAATGATATTTTGCCCCCCGTTTCTTTAAAATGGCAGTATATTGAAGATACAGC
AAGACGTATTTTTCAAATGTATAACTATAAAGAAATTAGGACTCCTATATTTGAGTATACAGAGTTATTTCAGCGGGGAA
TTGGTGAAACAACAGATATTGTTGAAAAAGAGATGTATACCTTTGAAGATAAAGGTGGACGGAGTATTACTTTAAGGCCC
GAAGGAACAGCTTCAGTGGTCAGGGCTTTTTTAGAACATAAAATTTATGGACAGGTTCAGCCTACTAAGTACTTTTATAT
AGGTCCCATGTTCAGGTATGAGAGACCACAGGCCGGTAGATTTAGGCAGTTTCACCAACTGGGGGTTGAAGCCTTTGGTT
CCAATGATCCTGCCCTTGATGCTGAAGTTATTGCCCTGGGACTCGATATTTTAAAACGGTTAGGCTTAACAGATGTAGAA
GTCTTTATCAATAGTATTGGTTGTCCAGAGTGTCGGGCAAGATATTCAGATGAACTAAAGCAATATTTAGAGTCACATCA
GGACAGGCTCTGTAAAGATTGTAAAGCAAGACTCAATAAAAATCCCCTGCGTATCCTGGATTGTAAAAATGAAGAATGTT
CACTGGTGATTAAAAATGCCCCTAAAATACTGGATTATTTATGTGATAATTGCAGGGTTCACTTTGAGGATGTTCAGGAA
TATCTGGACTTACTGGGTATTAAATACAGGGTTGATCCAACCCTGGTCAGGGGACTGGATTACTATACCAACACTGCCTT
TGAAATTAAATTTAAAGAACTGGGTGCTCAGGATGCTATTTTTGGTGGCGGTCGTTATAATGGGTTAACAGAAGAAATAG
GTAATAAGTCTATTCCGGGAATTGGTTTTGCTGTGGGAATTGAAAGGCTTATTCTTGCTCTTGATAAAAAGGGGATAAAG
TTACCTGTTAATGACAGTATTGATGTATACCTGGTTACAATTGGTGAACGAGCCAAGCGGGCGGCTTTTAACTATACATA
TTTATTAAGAGAATCAGGTATTACAGCAGAGATAGATTATCTGGGCAGAAGTATTAAAAGCCAGATGAAGTCTGCTGACA
GGACAGGTGCCAGTTATACTATTATAATCGGTGATAGTGAACTGGATTCAGGTAAAGCAACTGTAAAGAATATGAGGACC
GGTGAACAGGTTGAAATTATGCTTGCCAATCTTATAGAGGAAATGCAAAAGCTAGTATGA

Upstream 100 bases:

>100_bases
GTTGAAAAAGCCCAGACTAATAATGGCAGAACTGAAAATAAATAATTAATTTCCCAGGCTTTTAACACATCAATGAAAAT
TGTAATAAAAGGGTGATAAT

Downstream 100 bases:

>100_bases
AGGAGGAAGTTAATTGATGGCTGAGAGTATAAATGGACTTAAGAGAACACACTATGCCGGAAAGATTTCTACCCAACAAA
TAGGTAAAAAGGTAACTGTA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MDVKAPRGTNDILPPVSLKWQYIEDTARRIFQMYNYKEIRTPIFEYTELFQRGIGETTDIVEKEMYTFEDKGGRSITLRP
EGTASVVRAFLEHKIYGQVQPTKYFYIGPMFRYERPQAGRFRQFHQLGVEAFGSNDPALDAEVIALGLDILKRLGLTDVE
VFINSIGCPECRARYSDELKQYLESHQDRLCKDCKARLNKNPLRILDCKNEECSLVIKNAPKILDYLCDNCRVHFEDVQE
YLDLLGIKYRVDPTLVRGLDYYTNTAFEIKFKELGAQDAIFGGGRYNGLTEEIGNKSIPGIGFAVGIERLILALDKKGIK
LPVNDSIDVYLVTIGERAKRAAFNYTYLLRESGITAEIDYLGRSIKSQMKSADRTGASYTIIIGDSELDSGKATVKNMRT
GEQVEIMLANLIEEMQKLV

Sequences:

>Translated_419_residues
MDVKAPRGTNDILPPVSLKWQYIEDTARRIFQMYNYKEIRTPIFEYTELFQRGIGETTDIVEKEMYTFEDKGGRSITLRP
EGTASVVRAFLEHKIYGQVQPTKYFYIGPMFRYERPQAGRFRQFHQLGVEAFGSNDPALDAEVIALGLDILKRLGLTDVE
VFINSIGCPECRARYSDELKQYLESHQDRLCKDCKARLNKNPLRILDCKNEECSLVIKNAPKILDYLCDNCRVHFEDVQE
YLDLLGIKYRVDPTLVRGLDYYTNTAFEIKFKELGAQDAIFGGGRYNGLTEEIGNKSIPGIGFAVGIERLILALDKKGIK
LPVNDSIDVYLVTIGERAKRAAFNYTYLLRESGITAEIDYLGRSIKSQMKSADRTGASYTIIIGDSELDSGKATVKNMRT
GEQVEIMLANLIEEMQKLV
>Mature_419_residues
MDVKAPRGTNDILPPVSLKWQYIEDTARRIFQMYNYKEIRTPIFEYTELFQRGIGETTDIVEKEMYTFEDKGGRSITLRP
EGTASVVRAFLEHKIYGQVQPTKYFYIGPMFRYERPQAGRFRQFHQLGVEAFGSNDPALDAEVIALGLDILKRLGLTDVE
VFINSIGCPECRARYSDELKQYLESHQDRLCKDCKARLNKNPLRILDCKNEECSLVIKNAPKILDYLCDNCRVHFEDVQE
YLDLLGIKYRVDPTLVRGLDYYTNTAFEIKFKELGAQDAIFGGGRYNGLTEEIGNKSIPGIGFAVGIERLILALDKKGIK
LPVNDSIDVYLVTIGERAKRAAFNYTYLLRESGITAEIDYLGRSIKSQMKSADRTGASYTIIIGDSELDSGKATVKNMRT
GEQVEIMLANLIEEMQKLV

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=464, Percent_Identity=26.2931034482759, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI6996014, Length=460, Percent_Identity=24.7826086956522, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=44.7867298578199, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=24.7826086956522, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71993686, Length=458, Percent_Identity=24.235807860262, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6325290, Length=467, Percent_Identity=25.0535331905782, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24643061, Length=474, Percent_Identity=23.8396624472574, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24643059, Length=493, Percent_Identity=22.920892494929, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_HALOH (B8CXE6)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508960.1
- ProteinModelPortal:   B8CXE6
- SMR:   B8CXE6
- GeneID:   7313916
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_12150
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   CLSK2806404
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47685; Mature: 47685

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVKAPRGTNDILPPVSLKWQYIEDTARRIFQMYNYKEIRTPIFEYTELFQRGIGETTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCHHHHHHHHHHHCCCCHHHH
VEKEMYTFEDKGGRSITLRPEGTASVVRAFLEHKIYGQVQPTKYFYIGPMFRYERPQAGR
HHHHHHHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCCHH
FRQFHQLGVEAFGSNDPALDAEVIALGLDILKRLGLTDVEVFINSIGCPECRARYSDELK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
QYLESHQDRLCKDCKARLNKNPLRILDCKNEECSLVIKNAPKILDYLCDNCRVHFEDVQE
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHCCEEEHHHHHH
YLDLLGIKYRVDPTLVRGLDYYTNTAFEIKFKELGAQDAIFGGGRYNGLTEEIGNKSIPG
HHHHHCCEEECCHHHHHCCHHHCCCEEEEEHHHCCCCCCEECCCCCCCHHHHHCCCCCCC
IGFAVGIERLILALDKKGIKLPVNDSIDVYLVTIGERAKRAAFNYTYLLRESGITAEIDY
CHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCHHHHHHHCEEEEEECCCCEEEHHH
LGRSIKSQMKSADRTGASYTIIIGDSELDSGKATVKNMRTGEQVEIMLANLIEEMQKLV
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDVKAPRGTNDILPPVSLKWQYIEDTARRIFQMYNYKEIRTPIFEYTELFQRGIGETTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCHHHHHHHHHHHCCCCHHHH
VEKEMYTFEDKGGRSITLRPEGTASVVRAFLEHKIYGQVQPTKYFYIGPMFRYERPQAGR
HHHHHHHEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCCCCCCHH
FRQFHQLGVEAFGSNDPALDAEVIALGLDILKRLGLTDVEVFINSIGCPECRARYSDELK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
QYLESHQDRLCKDCKARLNKNPLRILDCKNEECSLVIKNAPKILDYLCDNCRVHFEDVQE
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHCCEEEHHHHHH
YLDLLGIKYRVDPTLVRGLDYYTNTAFEIKFKELGAQDAIFGGGRYNGLTEEIGNKSIPG
HHHHHCCEEECCHHHHHCCHHHCCCEEEEEHHHCCCCCCEECCCCCCCHHHHHCCCCCCC
IGFAVGIERLILALDKKGIKLPVNDSIDVYLVTIGERAKRAAFNYTYLLRESGITAEIDY
CHHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEECCHHHHHHHCEEEEEECCCCEEEHHH
LGRSIKSQMKSADRTGASYTIIIGDSELDSGKATVKNMRTGEQVEIMLANLIEEMQKLV
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA