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The map label for this gene is aspS

Identifier: 220932051

GI number: 220932051

Start: 1305972

End: 1307744

Strand: Reverse

Name: aspS

Synonym: Hore_12140

Alternate gene names: 220932051

Gene position: 1307744-1305972 (Counterclockwise)

Preceding gene: 220932052

Following gene: 220932049

Centisome position: 50.72

GC content: 40.61

Gene sequence:

>1773_bases
ATGGCTGAGAGTATAAATGGACTTAAGAGAACACACTATGCCGGAAAGATTTCTACCCAACAAATAGGTAAAAAGGTAAC
TGTAATGGGTTGGGTACAGAAGAGAAGAGATCATGGGGGAGTAATTTTTATTGACCTCAGGGATAGATCTGGTATTGTCC
AGGTGGTTTTTAATCCTGATAATAATGAGCGGGCTTTTAAAAAGGCTACTGAATTGAGGTCTGAGTACGTAATTGCTGTA
GAAGGAGAGGTTGAAAAGAGGCCGGAAGGTAACATTAATCCAGACATTCCCACCGGTGAAATAGAAATCAGGGGAAATAG
ATTAAGAATACTGGATGAATCTGAGACCCCTCCCTTTGAAATAGATGATGATATAAATGTAGGGGAGGATTTGCGGTTAA
AGTATAGATATCTTGACCTGAGGAGACCCCGCATGAAAAAGAATTTAGAACTCCGACACAGGGTCATGAAGACTACCCGG
GATTACCTTGATGACAATGGATTCTGGGAAGTTGAGACGCCGATTTTAACCCGTAGTACTCCGGAAGGAGCCAGGGATTA
TCTGGTGCCCAGTAGAATTCACCCCGGTCATTTTTATGCCCTGCCCCAGTCTCCCCAGCTTTTTAAACAGCTGCTAATGG
TAGGTGGGGTTGAAAGATATTTTCAGATAGCCCGTTGTTTCCGGGATGAAGATTTAAGGGCAAATAGACAACCGGAGTTT
ACCCAGATTGATATTGAAATGTCATTTATAGATAAAGAGGATGTTTTCTCACTGGTAAATGGTCTTATGAAAAGATTATT
TGCCCTTGTTGATATTGAGATTCCGGAAAAAATTCCTGTTATGCCTTACCAGGAAGCAATAGATAGGTTTGGTTCAGATA
AACCTGATTTAAGGTTTGGTATGGAACTAAAGGATGTTTCTGATGTAGTCAAAGATAGTGAATTCAGGGTTTTTAGCGGG
GTTGTAAAGGATGGAGGTCAGGTGAAGGGCATTAACTTTAAAGGTGGTGCCATCTCACCCAGGAGTAAGATTGATGGTTA
TACAGATTATGTAAAAATATTTGGAGCTAAAGGCCTTGCCTGGATTGCTTTAAAGGAAAATGGTCTTAAGTCACCGATTG
CCAAGTTTTTATCTGAAAAAGAGCTTGAAGGCATAAAAGAAAAAATGGGTGCCCAAACTGGAGACTTACTCCTGTTTGTA
GCCGATAAGCCCTCGATTGTCGCTGATGCTCTGGGGAACCTCAGACTTAAGATTGCCAGGGAAGAGGGTTTAATTCCTGA
TGGTGTTTATAAATTTGTCTGGGTAGTTGATTTTCCCTTGATGGAGTATGATGAGGATGAAGGTCGTTATGTAGCCAAAC
ACCACCCTTTCACTGCTCCTCTAGATGAAGATATTCCATTACTTGATAGTAATCCTGAAAAAATTAGATCCAAGGCCTAT
GATTTTGTATTAAATGGTGAAGAACTGGGTGGGGGAAGTATCCGTATTAATAATAAAGATCTCCAGATGAAAGTTTTTAA
TGCCTTAAATATAAGTGAAGAAAAAGCCAGTGACAAATTTGGGTTTTTATTGGAAGCCTTTAATTATGGGGCTCCACCCC
ATGGCGGTATAGCCTTTGGACTGGATCGTTTATTAATGGTATTAAGTGGTTCAGAATCCATGAGAGATGTTATTGCCTTC
CCCAAAACCCAGAAGGCATCATCACCATTAACAGAGGCTCCATCAACAGTTGCCGAGAAACAGCTTCGAGAACTCCATAT
AAAAATAGATTAG

Upstream 100 bases:

>100_bases
GCAACTGTAAAGAATATGAGGACCGGTGAACAGGTTGAAATTATGCTTGCCAATCTTATAGAGGAAATGCAAAAGCTAGT
ATGAAGGAGGAAGTTAATTG

Downstream 100 bases:

>100_bases
AAAACTAACACCTTCTGATTTCTTACTTAACTTTTTTTCTTTTGCCTGTTCTTTATATTATTTTAGGTTTCTGTTTATTA
AAAAGGGATGTTATATCATG

Product: Aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MAESINGLKRTHYAGKISTQQIGKKVTVMGWVQKRRDHGGVIFIDLRDRSGIVQVVFNPDNNERAFKKATELRSEYVIAV
EGEVEKRPEGNINPDIPTGEIEIRGNRLRILDESETPPFEIDDDINVGEDLRLKYRYLDLRRPRMKKNLELRHRVMKTTR
DYLDDNGFWEVETPILTRSTPEGARDYLVPSRIHPGHFYALPQSPQLFKQLLMVGGVERYFQIARCFRDEDLRANRQPEF
TQIDIEMSFIDKEDVFSLVNGLMKRLFALVDIEIPEKIPVMPYQEAIDRFGSDKPDLRFGMELKDVSDVVKDSEFRVFSG
VVKDGGQVKGINFKGGAISPRSKIDGYTDYVKIFGAKGLAWIALKENGLKSPIAKFLSEKELEGIKEKMGAQTGDLLLFV
ADKPSIVADALGNLRLKIAREEGLIPDGVYKFVWVVDFPLMEYDEDEGRYVAKHHPFTAPLDEDIPLLDSNPEKIRSKAY
DFVLNGEELGGGSIRINNKDLQMKVFNALNISEEKASDKFGFLLEAFNYGAPPHGGIAFGLDRLLMVLSGSESMRDVIAF
PKTQKASSPLTEAPSTVAEKQLRELHIKID

Sequences:

>Translated_590_residues
MAESINGLKRTHYAGKISTQQIGKKVTVMGWVQKRRDHGGVIFIDLRDRSGIVQVVFNPDNNERAFKKATELRSEYVIAV
EGEVEKRPEGNINPDIPTGEIEIRGNRLRILDESETPPFEIDDDINVGEDLRLKYRYLDLRRPRMKKNLELRHRVMKTTR
DYLDDNGFWEVETPILTRSTPEGARDYLVPSRIHPGHFYALPQSPQLFKQLLMVGGVERYFQIARCFRDEDLRANRQPEF
TQIDIEMSFIDKEDVFSLVNGLMKRLFALVDIEIPEKIPVMPYQEAIDRFGSDKPDLRFGMELKDVSDVVKDSEFRVFSG
VVKDGGQVKGINFKGGAISPRSKIDGYTDYVKIFGAKGLAWIALKENGLKSPIAKFLSEKELEGIKEKMGAQTGDLLLFV
ADKPSIVADALGNLRLKIAREEGLIPDGVYKFVWVVDFPLMEYDEDEGRYVAKHHPFTAPLDEDIPLLDSNPEKIRSKAY
DFVLNGEELGGGSIRINNKDLQMKVFNALNISEEKASDKFGFLLEAFNYGAPPHGGIAFGLDRLLMVLSGSESMRDVIAF
PKTQKASSPLTEAPSTVAEKQLRELHIKID
>Mature_589_residues
AESINGLKRTHYAGKISTQQIGKKVTVMGWVQKRRDHGGVIFIDLRDRSGIVQVVFNPDNNERAFKKATELRSEYVIAVE
GEVEKRPEGNINPDIPTGEIEIRGNRLRILDESETPPFEIDDDINVGEDLRLKYRYLDLRRPRMKKNLELRHRVMKTTRD
YLDDNGFWEVETPILTRSTPEGARDYLVPSRIHPGHFYALPQSPQLFKQLLMVGGVERYFQIARCFRDEDLRANRQPEFT
QIDIEMSFIDKEDVFSLVNGLMKRLFALVDIEIPEKIPVMPYQEAIDRFGSDKPDLRFGMELKDVSDVVKDSEFRVFSGV
VKDGGQVKGINFKGGAISPRSKIDGYTDYVKIFGAKGLAWIALKENGLKSPIAKFLSEKELEGIKEKMGAQTGDLLLFVA
DKPSIVADALGNLRLKIAREEGLIPDGVYKFVWVVDFPLMEYDEDEGRYVAKHHPFTAPLDEDIPLLDSNPEKIRSKAYD
FVLNGEELGGGSIRINNKDLQMKVFNALNISEEKASDKFGFLLEAFNYGAPPHGGIAFGLDRLLMVLSGSESMRDVIAFP
KTQKASSPLTEAPSTVAEKQLRELHIKID

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=592, Percent_Identity=42.5675675675676, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI5031815, Length=272, Percent_Identity=28.3088235294118, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI45439306, Length=303, Percent_Identity=25.7425742574257, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI194272210, Length=272, Percent_Identity=28.3088235294118, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI4758762, Length=252, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI226491502, Length=269, Percent_Identity=24.5353159851301, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1788173, Length=586, Percent_Identity=51.3651877133106, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1789256, Length=282, Percent_Identity=28.7234042553192, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790571, Length=258, Percent_Identity=29.0697674418605, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082379, Length=182, Percent_Identity=30.2197802197802, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=36.2876254180602, Blast_Score=327, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=261, Percent_Identity=27.2030651340996, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=261, Percent_Identity=27.2030651340996, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=261, Percent_Identity=27.2030651340996, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71984122, Length=326, Percent_Identity=27.3006134969325, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=307, Percent_Identity=23.7785016286645, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=629, Percent_Identity=34.181240063593, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6321807, Length=254, Percent_Identity=26.3779527559055, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=294, Percent_Identity=28.2312925170068, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=256, Percent_Identity=24.609375, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=36.3486842105263, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136276, Length=294, Percent_Identity=27.2108843537415, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=27.4900398406374, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=27.4900398406374, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_HALOH (B8CXE5)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508959.1
- ProteinModelPortal:   B8CXE5
- SMR:   B8CXE5
- GeneID:   7313915
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_12140
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66816; Mature: 66685

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESINGLKRTHYAGKISTQQIGKKVTVMGWVQKRRDHGGVIFIDLRDRSGIVQVVFNPD
CCCCCCHHHHHHCCCCCCHHHHCCEEEEEHHHHHHCCCCCEEEEEEECCCCEEEEEECCC
NNERAFKKATELRSEYVIAVEGEVEKRPEGNINPDIPTGEIEIRGNRLRILDESETPPFE
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCC
IDDDINVGEDLRLKYRYLDLRRPRMKKNLELRHRVMKTTRDYLDDNGFWEVETPILTRST
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCC
PEGARDYLVPSRIHPGHFYALPQSPQLFKQLLMVGGVERYFQIARCFRDEDLRANRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCE
TQIDIEMSFIDKEDVFSLVNGLMKRLFALVDIEIPEKIPVMPYQEAIDRFGSDKPDLRFG
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCCCCCEEC
MELKDVSDVVKDSEFRVFSGVVKDGGQVKGINFKGGAISPRSKIDGYTDYVKIFGAKGLA
CCHHHHHHHHCCCCHHEEHHHHCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHCCCCCEE
WIALKENGLKSPIAKFLSEKELEGIKEKMGAQTGDLLLFVADKPSIVADALGNLRLKIAR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCEEEEEEC
EEGLIPDGVYKFVWVVDFPLMEYDEDEGRYVAKHHPFTAPLDEDIPLLDSNPEKIRSKAY
CCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
DFVLNGEELGGGSIRINNKDLQMKVFNALNISEEKASDKFGFLLEAFNYGAPPHGGIAFG
EEEECCCCCCCCEEEECCCCEEEEEEHHCCCCHHHCCCHHHHHHHHHCCCCCCCCCHHHH
LDRLLMVLSGSESMRDVIAFPKTQKASSPLTEAPSTVAEKQLRELHIKID
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECC
>Mature Secondary Structure 
AESINGLKRTHYAGKISTQQIGKKVTVMGWVQKRRDHGGVIFIDLRDRSGIVQVVFNPD
CCCCCHHHHHHCCCCCCHHHHCCEEEEEHHHHHHCCCCCEEEEEEECCCCEEEEEECCC
NNERAFKKATELRSEYVIAVEGEVEKRPEGNINPDIPTGEIEIRGNRLRILDESETPPFE
CCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCC
IDDDINVGEDLRLKYRYLDLRRPRMKKNLELRHRVMKTTRDYLDDNGFWEVETPILTRST
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCC
PEGARDYLVPSRIHPGHFYALPQSPQLFKQLLMVGGVERYFQIARCFRDEDLRANRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCE
TQIDIEMSFIDKEDVFSLVNGLMKRLFALVDIEIPEKIPVMPYQEAIDRFGSDKPDLRFG
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCCCCCEEC
MELKDVSDVVKDSEFRVFSGVVKDGGQVKGINFKGGAISPRSKIDGYTDYVKIFGAKGLA
CCHHHHHHHHCCCCHHEEHHHHCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHCCCCCEE
WIALKENGLKSPIAKFLSEKELEGIKEKMGAQTGDLLLFVADKPSIVADALGNLRLKIAR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCEEEEEEC
EEGLIPDGVYKFVWVVDFPLMEYDEDEGRYVAKHHPFTAPLDEDIPLLDSNPEKIRSKAY
CCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
DFVLNGEELGGGSIRINNKDLQMKVFNALNISEEKASDKFGFLLEAFNYGAPPHGGIAFG
EEEECCCCCCCCEEEECCCCEEEEEEHHCCCCHHHCCCHHHHHHHHHCCCCCCCCCHHHH
LDRLLMVLSGSESMRDVIAFPKTQKASSPLTEAPSTVAEKQLRELHIKID
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA