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The map label for this gene is ygfA [C]

Identifier: 220932041

GI number: 220932041

Start: 1299014

End: 1299595

Strand: Reverse

Name: ygfA [C]

Synonym: Hore_12040

Alternate gene names: 220932041

Gene position: 1299595-1299014 (Counterclockwise)

Preceding gene: 220932042

Following gene: 220932040

Centisome position: 50.41

GC content: 37.46

Gene sequence:

>582_bases
GTGCAGACCAAGGCTGAATTGAGAAAGTTATTTATGGGATTTAGAAGTAGTATTAACCCTGAAAGAAGACATGAGTTGAG
TATTAGTATAAATAATAAATTTTTGAATTTACGTGAAGTGAGTGTGGCTCAAGAGATTATGTGTTATGTTTCTACTGGTA
GTGAGGTAGAAACCAGAGACTTAATAACTAAACTAATCGGTTATGGTAAACAGGTCCTTGTACCTTACTGCATTACTGAT
AAAAAGGAAATTAAGATTAGCCCTATTAAAGACCTTGAAAATGATCTTGTTGAAGGTGCCTACGGAATTCCGGAACCGCA
AAATCGTGTTGATACCCCCTTAAAAGGACTTGATATTGTTATTGTCCCCGGGATTGTTTTTTCCAGGGATGGTTATAGAA
TAGGTTATGGGGGAGGTTATTATGACAGGTTTCTGGTCAAACTACCAGACAATGTATTAACAGTTGGTTTTACTTACCAT
GCTTTACTTCTGGATAACCTTCCAATTGACCGTTATGACCAGCCTGTAGACATGGTAATAACGGAACGCGAAACATTGGT
AACCGGGGGTGGTAATTATTAA

Upstream 100 bases:

>100_bases
AGTTTTTTTTATGCCGGGTTTTAATTTATTTTTTCTTCATATTGGTGTAAATTTTTGATATTATATATAAGATAATATAT
TATATAAAGGGTGGTCAGGT

Downstream 100 bases:

>100_bases
TCTTTTTGAACAAAATTCTGAGAGTAATGACAAAAATAGACCACTGGCTTATCGAATGCGGCCGGGAAACCTCGATGAAT
TTTATGGACAGGAAGATGTG

Product: 5,10-methenyltetrahydrofolate synthetase

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MQTKAELRKLFMGFRSSINPERRHELSISINNKFLNLREVSVAQEIMCYVSTGSEVETRDLITKLIGYGKQVLVPYCITD
KKEIKISPIKDLENDLVEGAYGIPEPQNRVDTPLKGLDIVIVPGIVFSRDGYRIGYGGGYYDRFLVKLPDNVLTVGFTYH
ALLLDNLPIDRYDQPVDMVITERETLVTGGGNY

Sequences:

>Translated_193_residues
MQTKAELRKLFMGFRSSINPERRHELSISINNKFLNLREVSVAQEIMCYVSTGSEVETRDLITKLIGYGKQVLVPYCITD
KKEIKISPIKDLENDLVEGAYGIPEPQNRVDTPLKGLDIVIVPGIVFSRDGYRIGYGGGYYDRFLVKLPDNVLTVGFTYH
ALLLDNLPIDRYDQPVDMVITERETLVTGGGNY
>Mature_193_residues
MQTKAELRKLFMGFRSSINPERRHELSISINNKFLNLREVSVAQEIMCYVSTGSEVETRDLITKLIGYGKQVLVPYCITD
KKEIKISPIKDLENDLVEGAYGIPEPQNRVDTPLKGLDIVIVPGIVFSRDGYRIGYGGGYYDRFLVKLPDNVLTVGFTYH
ALLLDNLPIDRYDQPVDMVITERETLVTGGGNY

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Escherichia coli, GI1789278, Length=174, Percent_Identity=28.735632183908, Blast_Score=77, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17510857, Length=197, Percent_Identity=27.4111675126904, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321031, Length=203, Percent_Identity=24.6305418719212, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161077430, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 21821; Mature: 21821

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKAELRKLFMGFRSSINPERRHELSISINNKFLNLREVSVAQEIMCYVSTGSEVETRD
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEHHHHHHHHHHEEEECCCCCCHHHH
LITKLIGYGKQVLVPYCITDKKEIKISPIKDLENDLVEGAYGIPEPQNRVDTPLKGLDIV
HHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
IVPGIVFSRDGYRIGYGGGYYDRFLVKLPDNVLTVGFTYHALLLDNLPIDRYDQPVDMVI
EECCEEECCCCCEEECCCCHHHHEEEECCCCEEEEHHHEEEEECCCCCCCCCCCCCEEEE
TERETLVTGGGNY
ECCEEEEECCCCC
>Mature Secondary Structure
MQTKAELRKLFMGFRSSINPERRHELSISINNKFLNLREVSVAQEIMCYVSTGSEVETRD
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEHHHHHHHHHHEEEECCCCCCHHHH
LITKLIGYGKQVLVPYCITDKKEIKISPIKDLENDLVEGAYGIPEPQNRVDTPLKGLDIV
HHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
IVPGIVFSRDGYRIGYGGGYYDRFLVKLPDNVLTVGFTYHALLLDNLPIDRYDQPVDMVI
EECCEEECCCCCEEECCCCHHHHEEEECCCCEEEEHHHEEEEECCCCCCCCCCCCCEEEE
TERETLVTGGGNY
ECCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]