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The map label for this gene is hyfF [H]

Identifier: 220932042

GI number: 220932042

Start: 1299965

End: 1301485

Strand: Reverse

Name: hyfF [H]

Synonym: Hore_12050

Alternate gene names: 220932042

Gene position: 1301485-1299965 (Counterclockwise)

Preceding gene: 220932043

Following gene: 220932041

Centisome position: 50.48

GC content: 35.96

Gene sequence:

>1521_bases
ATGATTCCATTAATGGATTTAATTGATATTACTAATATAAATATGTACCTCGCATTGACCGCTTTTATTATTAATATGCT
AACAATTGTAGCCGGGTGTTATTACTTTAAAGAAGGGAAATATATATATATATTCCTGAACCTGGGTACATTAATAGCTT
CTCTGATCGTTATTTTAACTTCTGACTGGGTTATATTTTTAGTGGCCTGGGAACTTGTAACTTTATTTACTGCTATAATG
ATCTTTAGAAAAGATTTCAACCTGGGGGTTCAATATTTTTTAGTCCAGCTTGCAGGGACTTCCCTGTTACTACTGGCTGT
TGCCATAATTATAAATCATGGGTATGATAAGATAGGGTATTTAAATGAAGTCAGTTTACAGACTCTTTTAATTATTGGGG
TCGGAACTAAAAGTGCTCTGTTTGGCCTCCATTTCTGGTTGCCAGTAGTTCATAGTAAAGCTCCTTCTCCCATAAGTGCC
CTTCTCTCGGGTTGGGTTGTTAATCTTGGCTATATACTTCTGGCCAGAACTCTCCCCGGTGATAACCGCTTACTCTTTTT
TCTTGGGGTTTTAATGATAATTTATGGTGGGTTGAGGGCTCTTGCTTCCGGTGACTATAAACTAATACTTGCCTATAGTA
CTATAAGTCAACTGGGTTATATTGCCCTGGGGTTGGGAATTGGATCCAGTTATGGGAAGGCAGGGGCTTTAATTCATATA
TTAAGTCATGGTCTGGCCAAAACAGTGTTATTTATAGGTAGTGGCTACTGGGAACAGGAATACCGTGGTCGGGTTATATT
TAATTTTAAAAAACCTTTTTTAAAGCAACCAGGTAATTATATAAGTTCAGTAATGGCCTTATTGTCTTTAATGGGGATTA
TATTTTTCCCCGGTTATCTTAGCAAATATTATTTAAAATATAGCTTGCCAGGTGGTTTTGCAGGAGAGTTTTTAATTCAC
TTGACCGGAATAGTAACATTTTTATACGGATTCAGGCTCCTCTGGTTTGCTTTTTTTAAGGATTTAGGTAGTAATTTCAG
GTTAAATAAGGTCAAGATTGGTGACTTAACGGTATTAAGGTCCAGCTGGCCACAGATTACCGGAATTTTAATTATGTTAA
TTGTTATGTTAAAGCCTGATTTAGTTTATACCCGGCTTGTGCATGGCAAACCCACTCTTTTTACCTTTCATAATGTCATT
ATTCTTTTTGCCGATGCTACCACAGCAATTATTATCCTTGTGATTTTTAAAGGAATAAAAAAGTATTTAAAACCCTGGCC
TGATTTCAAAAAAATATTCAGGGCTCTGTTTGTGAGGGTTATTAAGTTATCCCGCAAACTGTACCGGACCCTGGATACAG
AAAAAGTATTTGAGGGGTATTTTTATAATTTTATAAGGAACCTGGCTGCTATTATTTACAGGGCAGTTTATGTTTCTTTT
CAATTTCAATTACTGTGGATTCCTGTTATATTCTTACTTTTACTGGTATATCTCAGCCTTGCAACGGCCAATGTTTTGTG
A

Upstream 100 bases:

>100_bases
TGGTTTTTTTTATTACAGATTTGATGGCCCATTGCTGGGTGCTGCTGAAGCATTACTTGATAATGGCAAATACATTAGCC
AGCTGCTGGGGAGATAAATC

Downstream 100 bases:

>100_bases
TAATATATATTTGAACTTGAGATATGTATGAACAGTAAGTTCTGCCATGTCCGTGATGGTTAATTTAGTTTTGGCCAACA
CCAAATAGAAAGGGAGCCTG

Product: NADH dehydrogenase I subunit M

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MIPLMDLIDITNINMYLALTAFIINMLTIVAGCYYFKEGKYIYIFLNLGTLIASLIVILTSDWVIFLVAWELVTLFTAIM
IFRKDFNLGVQYFLVQLAGTSLLLLAVAIIINHGYDKIGYLNEVSLQTLLIIGVGTKSALFGLHFWLPVVHSKAPSPISA
LLSGWVVNLGYILLARTLPGDNRLLFFLGVLMIIYGGLRALASGDYKLILAYSTISQLGYIALGLGIGSSYGKAGALIHI
LSHGLAKTVLFIGSGYWEQEYRGRVIFNFKKPFLKQPGNYISSVMALLSLMGIIFFPGYLSKYYLKYSLPGGFAGEFLIH
LTGIVTFLYGFRLLWFAFFKDLGSNFRLNKVKIGDLTVLRSSWPQITGILIMLIVMLKPDLVYTRLVHGKPTLFTFHNVI
ILFADATTAIIILVIFKGIKKYLKPWPDFKKIFRALFVRVIKLSRKLYRTLDTEKVFEGYFYNFIRNLAAIIYRAVYVSF
QFQLLWIPVIFLLLLVYLSLATANVL

Sequences:

>Translated_506_residues
MIPLMDLIDITNINMYLALTAFIINMLTIVAGCYYFKEGKYIYIFLNLGTLIASLIVILTSDWVIFLVAWELVTLFTAIM
IFRKDFNLGVQYFLVQLAGTSLLLLAVAIIINHGYDKIGYLNEVSLQTLLIIGVGTKSALFGLHFWLPVVHSKAPSPISA
LLSGWVVNLGYILLARTLPGDNRLLFFLGVLMIIYGGLRALASGDYKLILAYSTISQLGYIALGLGIGSSYGKAGALIHI
LSHGLAKTVLFIGSGYWEQEYRGRVIFNFKKPFLKQPGNYISSVMALLSLMGIIFFPGYLSKYYLKYSLPGGFAGEFLIH
LTGIVTFLYGFRLLWFAFFKDLGSNFRLNKVKIGDLTVLRSSWPQITGILIMLIVMLKPDLVYTRLVHGKPTLFTFHNVI
ILFADATTAIIILVIFKGIKKYLKPWPDFKKIFRALFVRVIKLSRKLYRTLDTEKVFEGYFYNFIRNLAAIIYRAVYVSF
QFQLLWIPVIFLLLLVYLSLATANVL
>Mature_506_residues
MIPLMDLIDITNINMYLALTAFIINMLTIVAGCYYFKEGKYIYIFLNLGTLIASLIVILTSDWVIFLVAWELVTLFTAIM
IFRKDFNLGVQYFLVQLAGTSLLLLAVAIIINHGYDKIGYLNEVSLQTLLIIGVGTKSALFGLHFWLPVVHSKAPSPISA
LLSGWVVNLGYILLARTLPGDNRLLFFLGVLMIIYGGLRALASGDYKLILAYSTISQLGYIALGLGIGSSYGKAGALIHI
LSHGLAKTVLFIGSGYWEQEYRGRVIFNFKKPFLKQPGNYISSVMALLSLMGIIFFPGYLSKYYLKYSLPGGFAGEFLIH
LTGIVTFLYGFRLLWFAFFKDLGSNFRLNKVKIGDLTVLRSSWPQITGILIMLIVMLKPDLVYTRLVHGKPTLFTFHNVI
ILFADATTAIIILVIFKGIKKYLKPWPDFKKIFRALFVRVIKLSRKLYRTLDTEKVFEGYFYNFIRNLAAIIYRAVYVSF
QFQLLWIPVIFLLLLVYLSLATANVL

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=318, Percent_Identity=28.9308176100629, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788831, Length=144, Percent_Identity=44.4444444444444, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788827, Length=317, Percent_Identity=28.7066246056782, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=286, Percent_Identity=29.020979020979, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788613, Length=258, Percent_Identity=28.6821705426357, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788829, Length=180, Percent_Identity=30, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 57167; Mature: 57167

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLMDLIDITNINMYLALTAFIINMLTIVAGCYYFKEGKYIYIFLNLGTLIASLIVILT
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEHHHHHHHHHHHHH
SDWVIFLVAWELVTLFTAIMIFRKDFNLGVQYFLVQLAGTSLLLLAVAIIINHGYDKIGY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LNEVSLQTLLIIGVGTKSALFGLHFWLPVVHSKAPSPISALLSGWVVNLGYILLARTLPG
CCHHCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCC
DNRLLFFLGVLMIIYGGLRALASGDYKLILAYSTISQLGYIALGLGIGSSYGKAGALIHI
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHEECCCCCCCCHHHHHHH
LSHGLAKTVLFIGSGYWEQEYRGRVIFNFKKPFLKQPGNYISSVMALLSLMGIIFFPGYL
HHHHHHHHHHHHCCCCCCHHHCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SKYYLKYSLPGGFAGEFLIHLTGIVTFLYGFRLLWFAFFKDLGSNFRLNKVKIGDLTVLR
HHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEH
SSWPQITGILIMLIVMLKPDLVYTRLVHGKPTLFTFHNVIILFADATTAIIILVIFKGIK
HCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECEEEEEECHHHHHHHHHHHHHHH
KYLKPWPDFKKIFRALFVRVIKLSRKLYRTLDTEKVFEGYFYNFIRNLAAIIYRAVYVSF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFQLLWIPVIFLLLLVYLSLATANVL
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPLMDLIDITNINMYLALTAFIINMLTIVAGCYYFKEGKYIYIFLNLGTLIASLIVILT
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEHHHHHHHHHHHHH
SDWVIFLVAWELVTLFTAIMIFRKDFNLGVQYFLVQLAGTSLLLLAVAIIINHGYDKIGY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LNEVSLQTLLIIGVGTKSALFGLHFWLPVVHSKAPSPISALLSGWVVNLGYILLARTLPG
CCHHCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECCCC
DNRLLFFLGVLMIIYGGLRALASGDYKLILAYSTISQLGYIALGLGIGSSYGKAGALIHI
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHEECCCCCCCCHHHHHHH
LSHGLAKTVLFIGSGYWEQEYRGRVIFNFKKPFLKQPGNYISSVMALLSLMGIIFFPGYL
HHHHHHHHHHHHCCCCCCHHHCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SKYYLKYSLPGGFAGEFLIHLTGIVTFLYGFRLLWFAFFKDLGSNFRLNKVKIGDLTVLR
HHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEH
SSWPQITGILIMLIVMLKPDLVYTRLVHGKPTLFTFHNVIILFADATTAIIILVIFKGIK
HCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECEEEEEECHHHHHHHHHHHHHHH
KYLKPWPDFKKIFRALFVRVIKLSRKLYRTLDTEKVFEGYFYNFIRNLAAIIYRAVYVSF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFQLLWIPVIFLLLLVYLSLATANVL
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]