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The map label for this gene is miaA

Identifier: 220932002

GI number: 220932002

Start: 1255438

End: 1256424

Strand: Reverse

Name: miaA

Synonym: Hore_11650

Alternate gene names: 220932002

Gene position: 1256424-1255438 (Counterclockwise)

Preceding gene: 220932003

Following gene: 220932001

Centisome position: 48.73

GC content: 35.66

Gene sequence:

>987_bases
TTGATTAAACCCCTGACAAATCATGGAAATAAGAATATATATCCTGATAATTCTACCCTTATCGTTATCCTGGGACCTAC
AGCTGTAGGAAAAACCAGTTTATCTTTGCAGCTTGCCCGGGATATAAATGGAGAGATTATATCAGCCGATTCCATGCAGA
TATATAAAGACATGGATATAGGTACTGCCAAGGCCAGTCAAAAAGAGCGGAATATAATTCCCCATTATATGATTGATATT
ATTAAACCAGATCAGGAGTTTTCAGTAGCTGAATACCAGGCCATGGTTGATAACTTAATACCCGGGATTGTATATCGAAA
TAAAGTCCCCATATTGGTGGGGGGAACAGGTTTATATATAAGGGCTGTTATAGAGGGATTTTTATTCCCTGAAATGGACA
AAAATATAGAATTAAGGCAAAAACTTCAAAAAGAGGCGCAACAGTATGGAAATAAATATGTTTATAACAAACTTAAAAAA
ATAGATCCTGAACTTGCTAAAAAACTCCATCCAAATGATTTGAGGAGAGTAATACGGGGAATAGAAGTGTATCACCAGAC
CGGAAAAACCATGACTTATTTTAAAAAAGAGAAACAAAAAAAAGGTGATCGTTACCGAAATCTGAAAATAGGGCTTTACC
GGGAGCGTGAAGAACTTTATAAACGAATTAATAAAAGGGTCGATATTATGATAGAACAGGGTCTCATTGATGAAGTTAAG
TACCTTTTAACAAAATACCCTGATTTAAGTAAAACAGCCAGACAGGGTCTCGGTTATAAAGAAATTATCGGGTATTTAAA
AAGAGAGTATGACAGGGAAGAGGCCATCAGGCTTTTAAAAAGAAACACAAGACGGTATGCAAAACGGCAATTGACCTGGT
TCAGGCGGGATCAGGATATAAACTGGTTTAACCTTTCAACCGGTGATTATAAGAAAATTTATTCAGAAATAAAAAAACTG
AGCAGGGACTTTTTACTTGACTTTTAA

Upstream 100 bases:

>100_bases
GATAACCCTTATCGCTGTCCTCACGGAAGACCGATAATAATACATATAACAGAGGATGATATTAATAAGGGAATGGGGCG
TAAATGAGGAGGTAATTATT

Downstream 100 bases:

>100_bases
AATAAATTATGTTTTAATACATTTATGATTAAATATAATAAATCTGTTGCCGAATCCCATTTTTTTGGGAACGGGGGACC
CGTTTTTTATGGGGTTAATC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MIKPLTNHGNKNIYPDNSTLIVILGPTAVGKTSLSLQLARDINGEIISADSMQIYKDMDIGTAKASQKERNIIPHYMIDI
IKPDQEFSVAEYQAMVDNLIPGIVYRNKVPILVGGTGLYIRAVIEGFLFPEMDKNIELRQKLQKEAQQYGNKYVYNKLKK
IDPELAKKLHPNDLRRVIRGIEVYHQTGKTMTYFKKEKQKKGDRYRNLKIGLYREREELYKRINKRVDIMIEQGLIDEVK
YLLTKYPDLSKTARQGLGYKEIIGYLKREYDREEAIRLLKRNTRRYAKRQLTWFRRDQDINWFNLSTGDYKKIYSEIKKL
SRDFLLDF

Sequences:

>Translated_328_residues
MIKPLTNHGNKNIYPDNSTLIVILGPTAVGKTSLSLQLARDINGEIISADSMQIYKDMDIGTAKASQKERNIIPHYMIDI
IKPDQEFSVAEYQAMVDNLIPGIVYRNKVPILVGGTGLYIRAVIEGFLFPEMDKNIELRQKLQKEAQQYGNKYVYNKLKK
IDPELAKKLHPNDLRRVIRGIEVYHQTGKTMTYFKKEKQKKGDRYRNLKIGLYREREELYKRINKRVDIMIEQGLIDEVK
YLLTKYPDLSKTARQGLGYKEIIGYLKREYDREEAIRLLKRNTRRYAKRQLTWFRRDQDINWFNLSTGDYKKIYSEIKKL
SRDFLLDF
>Mature_328_residues
MIKPLTNHGNKNIYPDNSTLIVILGPTAVGKTSLSLQLARDINGEIISADSMQIYKDMDIGTAKASQKERNIIPHYMIDI
IKPDQEFSVAEYQAMVDNLIPGIVYRNKVPILVGGTGLYIRAVIEGFLFPEMDKNIELRQKLQKEAQQYGNKYVYNKLKK
IDPELAKKLHPNDLRRVIRGIEVYHQTGKTMTYFKKEKQKKGDRYRNLKIGLYREREELYKRINKRVDIMIEQGLIDEVK
YLLTKYPDLSKTARQGLGYKEIIGYLKREYDREEAIRLLKRNTRRYAKRQLTWFRRDQDINWFNLSTGDYKKIYSEIKKL
SRDFLLDF

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=32.9032258064516, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1790613, Length=290, Percent_Identity=34.8275862068965, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=30.8970099667774, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=36.3321799307958, Blast_Score=173, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24649830, Length=328, Percent_Identity=32.0121951219512, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_HALOH (B8CX96)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508910.1
- ProteinModelPortal:   B8CX96
- SMR:   B8CX96
- GeneID:   7313863
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_11650
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK2806383
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 38658; Mature: 38658

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPLTNHGNKNIYPDNSTLIVILGPTAVGKTSLSLQLARDINGEIISADSMQIYKDMDI
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEECCCCCCCEEECCCHHHHHCCCC
GTAKASQKERNIIPHYMIDIIKPDQEFSVAEYQAMVDNLIPGIVYRNKVPILVGGTGLYI
CCCCCCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEECCCCCEEECCCHHHH
RAVIEGFLFPEMDKNIELRQKLQKEAQQYGNKYVYNKLKKIDPELAKKLHPNDLRRVIRG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHH
IEVYHQTGKTMTYFKKEKQKKGDRYRNLKIGLYREREELYKRINKRVDIMIEQGLIDEVK
HHHHHHCCCCHHHHHHHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHEEHHHCCHHHHHH
YLLTKYPDLSKTARQGLGYKEIIGYLKREYDREEAIRLLKRNTRRYAKRQLTWFRRDQDI
HHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NWFNLSTGDYKKIYSEIKKLSRDFLLDF
CEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKPLTNHGNKNIYPDNSTLIVILGPTAVGKTSLSLQLARDINGEIISADSMQIYKDMDI
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEECCCCCCCEEECCCHHHHHCCCC
GTAKASQKERNIIPHYMIDIIKPDQEFSVAEYQAMVDNLIPGIVYRNKVPILVGGTGLYI
CCCCCCHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEECCCCCEEECCCHHHH
RAVIEGFLFPEMDKNIELRQKLQKEAQQYGNKYVYNKLKKIDPELAKKLHPNDLRRVIRG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHH
IEVYHQTGKTMTYFKKEKQKKGDRYRNLKIGLYREREELYKRINKRVDIMIEQGLIDEVK
HHHHHHCCCCHHHHHHHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHEEHHHCCHHHHHH
YLLTKYPDLSKTARQGLGYKEIIGYLKREYDREEAIRLLKRNTRRYAKRQLTWFRRDQDI
HHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NWFNLSTGDYKKIYSEIKKLSRDFLLDF
CEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA