Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 220931747

GI number: 220931747

Start: 976006

End: 977505

Strand: Direct

Name: murE [H]

Synonym: Hore_09040

Alternate gene names: 220931747

Gene position: 976006-977505 (Clockwise)

Preceding gene: 220931746

Following gene: 220931748

Centisome position: 37.86

GC content: 37.2

Gene sequence:

>1500_bases
TTGAAAATATCGGAATTAATTAAAGATATAACACCAGTTGAAACCAGGGGAGACCTGGATAAAGAAATAGATAATATTGT
TTATGATTCCCGACAGGTTACTGATAATTCCCTTTTTATTTGTATAGAAGGTTTTAATACTGACGGGCATAAATATATAG
ATAAAGCGATTAAAAAAGGGGCTGTAGCTGTAGTTATAGAAAAAGACCTGGAAGTATATTCTGAAGATATTACCTATATA
CGGGTTAATGATAGCCGGGAAACTATGGGTTATTTAGCCTCTACTTTCTATAATTATCCTTTAAAAAACTTATCTTTAAT
AGGAGTAACCGGTACTAATGGTAAAACTACAACAACATACCTCATAAAAGCTATACTTGAAGAAGCCGGTTTTAATACTG
GTTTAGTAGGGACTATAAAAAATATAATTGGTGATGAAACTCTTCCAGCAACCAGAACTACCCCTGAATCACTGGATTTA
TACCGCCTTTTTGCCAGAATGGTTGACTCCGGGGTTACCCATGCTGTTATGGAGGTTTCTTCCCATGCCCTTGATCTTAA
AAGGGTTGAAGGTATGGAATTCAAAATAGGGGTATTCACAAATATTAGTCAGGACCATCTTGATTACCATAAGTCCCTTG
ATGAATACCTTAAAGCAAAAAGTCGCCTCTTCCGGCAACTGGAACCAGGAGGGTGGGCGGTTGTTAATATTGATGATTCT
CATTCAGATAAGATAATTAAGTCTACTACAGGTAATATTATAACTTATGGTATTGAAGGAGATTGTGATTTAAAGGCTAA
TGAAATTAATCTGTCGCCAACAGGTGTTTCATACCATGTTTCCGGTAAAGAGAGTTTTAAGATTGACCTTAATCTAACCG
GTCTTTTTAATGTTTATAACTCACTGGCTGCAATATCCTGTGGACATGTGCTGGGAATTAATGGAGAAACAATAAAAGCG
GGAATTGAAAAAGTCCAGGGAGTTGCCGGTAGGTTTGAACTGGTTAACGAAGGACAGGATTTTGCAGTTGTGGTGGATTA
CGCCCATACCCCTGATGGGATGGAAAATGTGCTAGAAACAGCCCGGGAATTTGTTAAAGGAAATATAATAGTTGTTTTTG
GTTGTGGTGGTGACAGAGATAAGGGAAAAAGACCACTGATGGGTAAAATTGCAACCCAACTGGGGGATTATTGTATAATT
ACCTCTGATAATCCCCGGTCTGAAGACCCCATGGAAATAATAAAGGATATTGAAAAAGGTATACAAGAAGAAGTGATTTC
AGATACACCCTATGTTAAAGAACCTGACCGCAGAAAAGCAATCTTCTCTGCTATAAACTCTGCCCGTAAGGATGATATGG
TAATTATTTTTGGAAAAGGGCATGAAACCTATCAGATTTTTAAGGATAAGACTATTCCTTTTGATGATAGAGAAGTAGCC
AGAGAAGCTTTGAAAAATAGAAAAAAGGGTGAAGACCATGATACCAATGAGTCTGGCTGA

Upstream 100 bases:

>100_bases
GCCTGGCAGCAGGGTTCCAGGTGGCACCACTATAATAGTTGAAGTCAGTGATGATTAAATTTAACCTGATGTGGTTAAAA
TAAATACGGGGGGAAGAACA

Downstream 100 bases:

>100_bases
GATTAGAAATGCTGTTTCTGGAAAAATAATTCAGGGAGACCCCAAGAGTAAAATAACTGATATATCTATAGACAGTAGGA
CTATTAATCCTGGTGATATG

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKISELIKDITPVETRGDLDKEIDNIVYDSRQVTDNSLFICIEGFNTDGHKYIDKAIKKGAVAVVIEKDLEVYSEDITYI
RVNDSRETMGYLASTFYNYPLKNLSLIGVTGTNGKTTTTYLIKAILEEAGFNTGLVGTIKNIIGDETLPATRTTPESLDL
YRLFARMVDSGVTHAVMEVSSHALDLKRVEGMEFKIGVFTNISQDHLDYHKSLDEYLKAKSRLFRQLEPGGWAVVNIDDS
HSDKIIKSTTGNIITYGIEGDCDLKANEINLSPTGVSYHVSGKESFKIDLNLTGLFNVYNSLAAISCGHVLGINGETIKA
GIEKVQGVAGRFELVNEGQDFAVVVDYAHTPDGMENVLETAREFVKGNIIVVFGCGGDRDKGKRPLMGKIATQLGDYCII
TSDNPRSEDPMEIIKDIEKGIQEEVISDTPYVKEPDRRKAIFSAINSARKDDMVIIFGKGHETYQIFKDKTIPFDDREVA
REALKNRKKGEDHDTNESG

Sequences:

>Translated_499_residues
MKISELIKDITPVETRGDLDKEIDNIVYDSRQVTDNSLFICIEGFNTDGHKYIDKAIKKGAVAVVIEKDLEVYSEDITYI
RVNDSRETMGYLASTFYNYPLKNLSLIGVTGTNGKTTTTYLIKAILEEAGFNTGLVGTIKNIIGDETLPATRTTPESLDL
YRLFARMVDSGVTHAVMEVSSHALDLKRVEGMEFKIGVFTNISQDHLDYHKSLDEYLKAKSRLFRQLEPGGWAVVNIDDS
HSDKIIKSTTGNIITYGIEGDCDLKANEINLSPTGVSYHVSGKESFKIDLNLTGLFNVYNSLAAISCGHVLGINGETIKA
GIEKVQGVAGRFELVNEGQDFAVVVDYAHTPDGMENVLETAREFVKGNIIVVFGCGGDRDKGKRPLMGKIATQLGDYCII
TSDNPRSEDPMEIIKDIEKGIQEEVISDTPYVKEPDRRKAIFSAINSARKDDMVIIFGKGHETYQIFKDKTIPFDDREVA
REALKNRKKGEDHDTNESG
>Mature_499_residues
MKISELIKDITPVETRGDLDKEIDNIVYDSRQVTDNSLFICIEGFNTDGHKYIDKAIKKGAVAVVIEKDLEVYSEDITYI
RVNDSRETMGYLASTFYNYPLKNLSLIGVTGTNGKTTTTYLIKAILEEAGFNTGLVGTIKNIIGDETLPATRTTPESLDL
YRLFARMVDSGVTHAVMEVSSHALDLKRVEGMEFKIGVFTNISQDHLDYHKSLDEYLKAKSRLFRQLEPGGWAVVNIDDS
HSDKIIKSTTGNIITYGIEGDCDLKANEINLSPTGVSYHVSGKESFKIDLNLTGLFNVYNSLAAISCGHVLGINGETIKA
GIEKVQGVAGRFELVNEGQDFAVVVDYAHTPDGMENVLETAREFVKGNIIVVFGCGGDRDKGKRPLMGKIATQLGDYCII
TSDNPRSEDPMEIIKDIEKGIQEEVISDTPYVKEPDRRKAIFSAINSARKDDMVIIFGKGHETYQIFKDKTIPFDDREVA
REALKNRKKGEDHDTNESG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=476, Percent_Identity=36.9747899159664, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1786274, Length=341, Percent_Identity=26.6862170087977, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786279, Length=282, Percent_Identity=25.531914893617, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 55368; Mature: 55368

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01072 SLH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISELIKDITPVETRGDLDKEIDNIVYDSRQVTDNSLFICIEGFNTDGHKYIDKAIKKG
CCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECCCEEEEEEECCCCCCHHHHHHHHHCC
AVAVVIEKDLEVYSEDITYIRVNDSRETMGYLASTFYNYPLKNLSLIGVTGTNGKTTTTY
CEEEEEECCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHH
LIKAILEEAGFNTGLVGTIKNIIGDETLPATRTTPESLDLYRLFARMVDSGVTHAVMEVS
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
SHALDLKRVEGMEFKIGVFTNISQDHLDYHKSLDEYLKAKSRLFRQLEPGGWAVVNIDDS
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
HSDKIIKSTTGNIITYGIEGDCDLKANEINLSPTGVSYHVSGKESFKIDLNLTGLFNVYN
CCCCEEEECCCCEEEEECCCCCCCEECEEECCCCCEEEEECCCCEEEEEEEEHHHHHHHH
SLAAISCGHVLGINGETIKAGIEKVQGVAGRFELVNEGQDFAVVVDYAHTPDGMENVLET
HHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCHHHHHHHH
AREFVKGNIIVVFGCGGDRDKGKRPLMGKIATQLGDYCIITSDNPRSEDPMEIIKDIEKG
HHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
IQEEVISDTPYVKEPDRRKAIFSAINSARKDDMVIIFGKGHETYQIFKDKTIPFDDREVA
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHH
REALKNRKKGEDHDTNESG
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKISELIKDITPVETRGDLDKEIDNIVYDSRQVTDNSLFICIEGFNTDGHKYIDKAIKKG
CCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEECCCEEEEEEECCCCCCHHHHHHHHHCC
AVAVVIEKDLEVYSEDITYIRVNDSRETMGYLASTFYNYPLKNLSLIGVTGTNGKTTTTY
CEEEEEECCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHH
LIKAILEEAGFNTGLVGTIKNIIGDETLPATRTTPESLDLYRLFARMVDSGVTHAVMEVS
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
SHALDLKRVEGMEFKIGVFTNISQDHLDYHKSLDEYLKAKSRLFRQLEPGGWAVVNIDDS
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
HSDKIIKSTTGNIITYGIEGDCDLKANEINLSPTGVSYHVSGKESFKIDLNLTGLFNVYN
CCCCEEEECCCCEEEEECCCCCCCEECEEECCCCCEEEEECCCCEEEEEEEEHHHHHHHH
SLAAISCGHVLGINGETIKAGIEKVQGVAGRFELVNEGQDFAVVVDYAHTPDGMENVLET
HHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEEEECCCCCHHHHHHHH
AREFVKGNIIVVFGCGGDRDKGKRPLMGKIATQLGDYCIITSDNPRSEDPMEIIKDIEKG
HHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHH
IQEEVISDTPYVKEPDRRKAIFSAINSARKDDMVIIFGKGHETYQIFKDKTIPFDDREVA
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHH
REALKNRKKGEDHDTNESG
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]