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The map label for this gene is murF [H]

Identifier: 220931748

GI number: 220931748

Start: 977483

End: 978868

Strand: Direct

Name: murF [H]

Synonym: Hore_09050

Alternate gene names: 220931748

Gene position: 977483-978868 (Clockwise)

Preceding gene: 220931747

Following gene: 220931749

Centisome position: 37.91

GC content: 40.12

Gene sequence:

>1386_bases
ATGATACCAATGAGTCTGGCTGAGATTAGAAATGCTGTTTCTGGAAAAATAATTCAGGGAGACCCCAAGAGTAAAATAAC
TGATATATCTATAGACAGTAGGACTATTAATCCTGGTGATATGTTTGTTGCCATTATTGGGGAACGTTTTGATGGTCATA
GTTTTATTCCTGAAGCGGTTTCGAAAGGGGCCAGAGCTTTAATTGTGGATCACAGTATAAAGGCCTATCCAGGGGTAGGA
ATCATCCTTGTCGAGGATACTACCAGGGCCCTCCAGGATCTGGCCCGGTATCACCGGATGTTAATAAAGGGTTTAACGGT
AATCGGTATAACCGGTAGTGCCGGCAAAACTACTACCAAAGATATGATTGCTTCAGTATTGAAGGAAAAATATAATGTTA
CTAAAACCAGGGGTAATTTAAATAATTACTATGGTCTTCCCCTGACATTGCTTTCATTAGATGGGGATGAGGAGATTGCT
GTCCTCGAAATGGGAATGAGTAAACTGGGGGAGATAGAACTTCTGGCCAGTATTTCCAAACCAGATATAGGTGTTATCAC
TAACGTGGGTCCCACTCATCTTGAAAATTTAAAGACAGTCGGTAATGTGGCCCGGGGTAAGAGGGAGTTGATAGAAGGAC
TTCCTACCAATGGTCTGGCAATTCTTAACTATGATAACAAATATGTCAGGGACATGAAGGATTATGTAGAGGGGAAAAAA
GTAATATATTATGGGTTTTCTCCTGAGGCCGATGTTTATGCTGAACACATAAAAGTTGATGAAAAAAATAAAAATATAAG
TTTTATTGCCCATTATAAGACCGAAAAAGAAAGAATTACTTTACAAAAACCGGGAAAGCATAATGTCTATAATGCCCTGG
CGGCCATCGCAGTAGCCCGTTATTTAAGGGTGGACTGGGGTGGTATAAAAAATGGTCTGTATAATATTGATCTGTCATCA
TTAAGATGGGATGTCAGAGATTTAGGTAACGAGATTACCCTGATAAACGACACCTATAATGCCAATCCTCTTTCCATGAA
AGCTGCTATTGAGGCCACTTTTGATATAGCACAGGAAAGAGTGATAACCGTTCTTGGAGGTATGCTCGAACTCGGCGACA
TGGAAGAGAAGGCCCATGTTGACCTTGGTAGATATGTTGCAGAAAGAAATGTAGATGTTTTAATTACAGTTGGAGAACTG
GGGAGGTTGACGGCCAGGAGTGCCGAAAAAGAGGGTATGAAGAACGTTTATCAGGCTGATAATAATCAGGAAGCCTCCAG
AGTGTTACTGGATTTAATAAAAAAAGGTGATACAATTTTGGTTAAGGGTTCAAGAGGAAACAAAATGGAAGAAATAGTTA
AGATGGTCAGTTCACAGGAGGATTAA

Upstream 100 bases:

>100_bases
GGGCATGAAACCTATCAGATTTTTAAGGATAAGACTATTCCTTTTGATGATAGAGAAGTAGCCAGAGAAGCTTTGAAAAA
TAGAAAAAAGGGTGAAGACC

Downstream 100 bases:

>100_bases
AACTATGGAATATTTTGTAGCCCTGTGTGTGCCGTTTTTAATATTGATGATATTTGGACGGGGGTTAATCAAATTACTAA
AAATATTAAATTTTGGTCAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIPMSLAEIRNAVSGKIIQGDPKSKITDISIDSRTINPGDMFVAIIGERFDGHSFIPEAVSKGARALIVDHSIKAYPGVG
IILVEDTTRALQDLARYHRMLIKGLTVIGITGSAGKTTTKDMIASVLKEKYNVTKTRGNLNNYYGLPLTLLSLDGDEEIA
VLEMGMSKLGEIELLASISKPDIGVITNVGPTHLENLKTVGNVARGKRELIEGLPTNGLAILNYDNKYVRDMKDYVEGKK
VIYYGFSPEADVYAEHIKVDEKNKNISFIAHYKTEKERITLQKPGKHNVYNALAAIAVARYLRVDWGGIKNGLYNIDLSS
LRWDVRDLGNEITLINDTYNANPLSMKAAIEATFDIAQERVITVLGGMLELGDMEEKAHVDLGRYVAERNVDVLITVGEL
GRLTARSAEKEGMKNVYQADNNQEASRVLLDLIKKGDTILVKGSRGNKMEEIVKMVSSQED

Sequences:

>Translated_461_residues
MIPMSLAEIRNAVSGKIIQGDPKSKITDISIDSRTINPGDMFVAIIGERFDGHSFIPEAVSKGARALIVDHSIKAYPGVG
IILVEDTTRALQDLARYHRMLIKGLTVIGITGSAGKTTTKDMIASVLKEKYNVTKTRGNLNNYYGLPLTLLSLDGDEEIA
VLEMGMSKLGEIELLASISKPDIGVITNVGPTHLENLKTVGNVARGKRELIEGLPTNGLAILNYDNKYVRDMKDYVEGKK
VIYYGFSPEADVYAEHIKVDEKNKNISFIAHYKTEKERITLQKPGKHNVYNALAAIAVARYLRVDWGGIKNGLYNIDLSS
LRWDVRDLGNEITLINDTYNANPLSMKAAIEATFDIAQERVITVLGGMLELGDMEEKAHVDLGRYVAERNVDVLITVGEL
GRLTARSAEKEGMKNVYQADNNQEASRVLLDLIKKGDTILVKGSRGNKMEEIVKMVSSQED
>Mature_461_residues
MIPMSLAEIRNAVSGKIIQGDPKSKITDISIDSRTINPGDMFVAIIGERFDGHSFIPEAVSKGARALIVDHSIKAYPGVG
IILVEDTTRALQDLARYHRMLIKGLTVIGITGSAGKTTTKDMIASVLKEKYNVTKTRGNLNNYYGLPLTLLSLDGDEEIA
VLEMGMSKLGEIELLASISKPDIGVITNVGPTHLENLKTVGNVARGKRELIEGLPTNGLAILNYDNKYVRDMKDYVEGKK
VIYYGFSPEADVYAEHIKVDEKNKNISFIAHYKTEKERITLQKPGKHNVYNALAAIAVARYLRVDWGGIKNGLYNIDLSS
LRWDVRDLGNEITLINDTYNANPLSMKAAIEATFDIAQERVITVLGGMLELGDMEEKAHVDLGRYVAERNVDVLITVGEL
GRLTARSAEKEGMKNVYQADNNQEASRVLLDLIKKGDTILVKGSRGNKMEEIVKMVSSQED

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=458, Percent_Identity=34.061135371179, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50839; Mature: 50839

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPMSLAEIRNAVSGKIIQGDPKSKITDISIDSRTINPGDMFVAIIGERFDGHSFIPEAV
CCCCCHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHH
SKGARALIVDHSIKAYPGVGIILVEDTTRALQDLARYHRMLIKGLTVIGITGSAGKTTTK
CCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
DMIASVLKEKYNVTKTRGNLNNYYGLPLTLLSLDGDEEIAVLEMGMSKLGEIELLASISK
HHHHHHHHHHHCCEECCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHCCCEEEEEECCC
PDIGVITNVGPTHLENLKTVGNVARGKRELIEGLPTNGLAILNYDNKYVRDMKDYVEGKK
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCE
VIYYGFSPEADVYAEHIKVDEKNKNISFIAHYKTEKERITLQKPGKHNVYNALAAIAVAR
EEEECCCCCHHHHHHHEEEECCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHH
YLRVDWGGIKNGLYNIDLSSLRWDVRDLGNEITLINDTYNANPLSMKAAIEATFDIAQER
HHHCCCCCCCCCEEEEEHHHHEEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
VITVLGGMLELGDMEEKAHVDLGRYVAERNVDVLITVGELGRLTARSAEKEGMKNVYQAD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
NNQEASRVLLDLIKKGDTILVKGSRGNKMEEIVKMVSSQED
CCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPMSLAEIRNAVSGKIIQGDPKSKITDISIDSRTINPGDMFVAIIGERFDGHSFIPEAV
CCCCCHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHH
SKGARALIVDHSIKAYPGVGIILVEDTTRALQDLARYHRMLIKGLTVIGITGSAGKTTTK
CCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
DMIASVLKEKYNVTKTRGNLNNYYGLPLTLLSLDGDEEIAVLEMGMSKLGEIELLASISK
HHHHHHHHHHHCCEECCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHCCCEEEEEECCC
PDIGVITNVGPTHLENLKTVGNVARGKRELIEGLPTNGLAILNYDNKYVRDMKDYVEGKK
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCE
VIYYGFSPEADVYAEHIKVDEKNKNISFIAHYKTEKERITLQKPGKHNVYNALAAIAVAR
EEEECCCCCHHHHHHHEEEECCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHH
YLRVDWGGIKNGLYNIDLSSLRWDVRDLGNEITLINDTYNANPLSMKAAIEATFDIAQER
HHHCCCCCCCCCEEEEEHHHHEEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
VITVLGGMLELGDMEEKAHVDLGRYVAERNVDVLITVGELGRLTARSAEKEGMKNVYQAD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
NNQEASRVLLDLIKKGDTILVKGSRGNKMEEIVKMVSSQED
CCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]