Click here to switch to the map view.

The map label for this gene is aksA [H]

Identifier: 220931649

GI number: 220931649

Start: 865337

End: 866494

Strand: Direct

Name: aksA [H]

Synonym: Hore_08050

Alternate gene names: 220931649

Gene position: 865337-866494 (Clockwise)

Preceding gene: 220931648

Following gene: 220931650

Centisome position: 33.56

GC content: 39.98

Gene sequence:

>1158_bases
ATGGGAAGGAAAATAAAGATTGTTGATACTACATTACGTGATGGTGAGCAGACAGCAGGTGTTGTTTTTGCCAATGAAGA
GAAAATAAGGATTGCCAAACTCCTGGCCGAGATCGGGGTACAGCAGATTGAGGCCGGGATTCCGGTTATGGGTGGTGATG
AGAAAAAGGCAATAAAAAGTATTGTAGAAGCAGATCTCGATGTCAGTGTCATGGCCTGGAACCGGGCAGTTATTTCAGAT
ATAAAAGAGTCAATTGATTGTGGGGTTGATGCTGTTGCTATTTCTATATCTACTTCTGATATTCATATTAAACATAAACT
AAAAAGAGACCGGGACTGGGTCCTGAAAAACATGGTGGAGGCAACCAGGTTTGCTAAAAAACATAACTTATATGTGTCTG
TCAATGCTGAAGATTCTTCCCGGACAGATATGGATTTTTTATTAAGGTTTGCTAAAGAAGCTAAAGAAGCCGGAGCTGAC
CGTCTCCGTTATTGTGATACTGTAGGAACAATGGAGCCTTTTCAGCTTTATGAACAGGTCAAATTAATACGAAACGAAAT
AGGAATTGATGTAGAGATGCATACCCATAATGATTTTGGCCTGGCTACTGCCAATGCTTTAGCTGGAGTTAAAGCCGGTG
CCAATTATGTAGGGGTTACCGTAGGCGGTCTTGGAGAGCGGGCCGGGAATGCAGCCCTTGAAGAAGTAGTTATGGCTTTA
AAATATCTATATAATATTGATCTCAATTTTAAGACAAGTAAATTCAGGGAACTTAATGAATATGTCGGAATGGCTTCTAA
CCGCCCTGTTCCTGCCTGGAAACCTATTGTAGGCAGTAACATGTTTGCTCATGAATCCGGTATTCATACCGATGGGGTTA
TTAAAAATGCCAGTACTTATGAAATATTTTCACCAGAAGAAGTGGGGTTAACAAGACAGATTGTTATCGGGAAACACTCG
GGTAGAAATGCCATCATAAATAAGTTTAAGGAATATGGAATAAGCTTAAATAATGATGAGGCCACTGAAATCCTTAAAGA
AGTCAGATCCCTTGCCGTCAAATTAAAACGGGCTTTATTTGATAAAGAGCTTGTTTATATCTATCAGGACCTTAAACAGG
AAAGAGGAGAAGAATGGGAAGAGGAAAAACTGTCATAG

Upstream 100 bases:

>100_bases
TCTTTTATGAATAAAAAAACATAGTTAAGAATATATATTATTGGGGATTTATAGTTTTTTAAGTAGATAGAATAGAGCAG
CTTAGAAAGGGGTTGTAATC

Downstream 100 bases:

>100_bases
ATAAATATTGAATACCCATTCCTGTCAGGATGTGAAGAATCGTGGGTTATTACTGATATAGGTTTTATAATAGGCCAGGC
ATTTAGAGACCAGGTCCCTC

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MGRKIKIVDTTLRDGEQTAGVVFANEEKIRIAKLLAEIGVQQIEAGIPVMGGDEKKAIKSIVEADLDVSVMAWNRAVISD
IKESIDCGVDAVAISISTSDIHIKHKLKRDRDWVLKNMVEATRFAKKHNLYVSVNAEDSSRTDMDFLLRFAKEAKEAGAD
RLRYCDTVGTMEPFQLYEQVKLIRNEIGIDVEMHTHNDFGLATANALAGVKAGANYVGVTVGGLGERAGNAALEEVVMAL
KYLYNIDLNFKTSKFRELNEYVGMASNRPVPAWKPIVGSNMFAHESGIHTDGVIKNASTYEIFSPEEVGLTRQIVIGKHS
GRNAIINKFKEYGISLNNDEATEILKEVRSLAVKLKRALFDKELVYIYQDLKQERGEEWEEEKLS

Sequences:

>Translated_385_residues
MGRKIKIVDTTLRDGEQTAGVVFANEEKIRIAKLLAEIGVQQIEAGIPVMGGDEKKAIKSIVEADLDVSVMAWNRAVISD
IKESIDCGVDAVAISISTSDIHIKHKLKRDRDWVLKNMVEATRFAKKHNLYVSVNAEDSSRTDMDFLLRFAKEAKEAGAD
RLRYCDTVGTMEPFQLYEQVKLIRNEIGIDVEMHTHNDFGLATANALAGVKAGANYVGVTVGGLGERAGNAALEEVVMAL
KYLYNIDLNFKTSKFRELNEYVGMASNRPVPAWKPIVGSNMFAHESGIHTDGVIKNASTYEIFSPEEVGLTRQIVIGKHS
GRNAIINKFKEYGISLNNDEATEILKEVRSLAVKLKRALFDKELVYIYQDLKQERGEEWEEEKLS
>Mature_384_residues
GRKIKIVDTTLRDGEQTAGVVFANEEKIRIAKLLAEIGVQQIEAGIPVMGGDEKKAIKSIVEADLDVSVMAWNRAVISDI
KESIDCGVDAVAISISTSDIHIKHKLKRDRDWVLKNMVEATRFAKKHNLYVSVNAEDSSRTDMDFLLRFAKEAKEAGADR
LRYCDTVGTMEPFQLYEQVKLIRNEIGIDVEMHTHNDFGLATANALAGVKAGANYVGVTVGGLGERAGNAALEEVVMALK
YLYNIDLNFKTSKFRELNEYVGMASNRPVPAWKPIVGSNMFAHESGIHTDGVIKNASTYEIFSPEEVGLTRQIVIGKHSG
RNAIINKFKEYGISLNNDEATEILKEVRSLAVKLKRALFDKELVYIYQDLKQERGEEWEEEKLS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=352, Percent_Identity=36.6477272727273, Blast_Score=206, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320071, Length=355, Percent_Identity=31.830985915493, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320019, Length=353, Percent_Identity=32.2946175637394, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324225, Length=427, Percent_Identity=22.9508196721311, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324682, Length=319, Percent_Identity=23.5109717868339, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42927; Mature: 42795

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKIKIVDTTLRDGEQTAGVVFANEEKIRIAKLLAEIGVQQIEAGIPVMGGDEKKAIKS
CCCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHH
IVEADLDVSVMAWNRAVISDIKESIDCGVDAVAISISTSDIHIKHKLKRDRDWVLKNMVE
HHHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEHHHHHCCHHHHHHHHHH
ATRFAKKHNLYVSVNAEDSSRTDMDFLLRFAKEAKEAGADRLRYCDTVGTMEPFQLYEQV
HHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
KLIRNEIGIDVEMHTHNDFGLATANALAGVKAGANYVGVTVGGLGERAGNAALEEVVMAL
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
KYLYNIDLNFKTSKFRELNEYVGMASNRPVPAWKPIVGSNMFAHESGIHTDGVIKNASTY
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEE
EIFSPEEVGLTRQIVIGKHSGRNAIINKFKEYGISLNNDEATEILKEVRSLAVKLKRALF
EEECCHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKELVYIYQDLKQERGEEWEEEKLS
HHHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
GRKIKIVDTTLRDGEQTAGVVFANEEKIRIAKLLAEIGVQQIEAGIPVMGGDEKKAIKS
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHH
IVEADLDVSVMAWNRAVISDIKESIDCGVDAVAISISTSDIHIKHKLKRDRDWVLKNMVE
HHHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEHHHHHCCHHHHHHHHHH
ATRFAKKHNLYVSVNAEDSSRTDMDFLLRFAKEAKEAGADRLRYCDTVGTMEPFQLYEQV
HHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
KLIRNEIGIDVEMHTHNDFGLATANALAGVKAGANYVGVTVGGLGERAGNAALEEVVMAL
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
KYLYNIDLNFKTSKFRELNEYVGMASNRPVPAWKPIVGSNMFAHESGIHTDGVIKNASTY
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEE
EIFSPEEVGLTRQIVIGKHSGRNAIINKFKEYGISLNNDEATEILKEVRSLAVKLKRALF
EEECCHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DKELVYIYQDLKQERGEEWEEEKLS
HHHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]