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The map label for this gene is leuB [H]

Identifier: 220931650

GI number: 220931650

Start: 866688

End: 867683

Strand: Direct

Name: leuB [H]

Synonym: Hore_08060

Alternate gene names: 220931650

Gene position: 866688-867683 (Clockwise)

Preceding gene: 220931649

Following gene: 220931652

Centisome position: 33.62

GC content: 39.76

Gene sequence:

>996_bases
ATGATAACAGTAACTTTGATTCCTGGAGATGGAATTGGACCTGAAATAACGGACGTTGTGGTTGAAGTTTTTGAAGCACT
GGGGGTTGATGTAGACTGGGAAGTAGTTAATGCCGGCAAGTCGGTAATGGATAAATACGGCACCCCACTTCCTGATGAAG
TAATTGAATCCATTAAGAAAAATAAAGTAGCCTTAAAAGGGCCTATTACTACACCTGTCGGATCTGGTTTTCGTAGTGTT
AATGTAGCTATAAGAAAAAAGCTCAATTTATATGCTAATTTACGACCTGTTGAGAGTTTACCGGGGCTTAAAACCCGTTA
TGATAAGGTTAATCTTGTTGTAGTTAGAGAAAATACTGAAGGATTATATAGCGGGGTAGAACATAAAATTAGTGATTTTG
CTGCCGAAAGTATAAAAATTATTACCAGAAAGGCGAGTGAGAGGATTATAGAATTTGCCTTCAGTTATGCTAAAAAAAAT
GACCGTCAGCAGGTTACTGCGGTTCACAAGGCCAACATAATGAAAATTTCTGATGGGTTATTTCTGGATACGGCCCGTAA
GGTGGCTGAAAAATACCCTGGTATTGAGTACAATGAGCGAATTATAGATAATATGTGTATGCAACTGGTTCAAAATCCTG
AAGATTATGATGTTCTGGTAATGCCTAATTTTTACGGAGATATAGTATCAGATCTGGGGGCAGGTCTGGTTGGAGGGCTT
GGTGTAACCCCGGGGGCAAATATTGGAGATGAAATTGCAGTTTTTGAAGCAGTACATGGTAGTGCCCCTGATATTGCCGG
AAAGGGAGTAGCCAATCCTGTGGCTATTTTACGCTCAAGTATTATGTTATTAAATCATATCGGTGAAAGTGAAAAAGCCA
GTAAACTTGACCAGGCTATTAAGAAAACCCTGGTTAAAGGTAAGGGTCTAACTCCGGACCTCGGTGGTCAGGGAACCACC
CGGAGCTTTAAAGACCAGATAATTAATAATATCTAA

Upstream 100 bases:

>100_bases
GTCCCTCTGGTTACAAAATGTGCTGGAATTGGGGCTGTTATTACAAGGTCTTGAGGGTAAATTAAGAGTATGAAGCCTTA
AATGTTTAAGGGGGGTTAAA

Downstream 100 bases:

>100_bases
TGACACCACAGGCGAATCTTGGACCAGAGTTCCCGGCTGGTTGACTCCGGTAATCATCGGGATACTCATGAATAATAACT
GACCTTCCTATTATATCTTT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MITVTLIPGDGIGPEITDVVVEVFEALGVDVDWEVVNAGKSVMDKYGTPLPDEVIESIKKNKVALKGPITTPVGSGFRSV
NVAIRKKLNLYANLRPVESLPGLKTRYDKVNLVVVRENTEGLYSGVEHKISDFAAESIKIITRKASERIIEFAFSYAKKN
DRQQVTAVHKANIMKISDGLFLDTARKVAEKYPGIEYNERIIDNMCMQLVQNPEDYDVLVMPNFYGDIVSDLGAGLVGGL
GVTPGANIGDEIAVFEAVHGSAPDIAGKGVANPVAILRSSIMLLNHIGESEKASKLDQAIKKTLVKGKGLTPDLGGQGTT
RSFKDQIINNI

Sequences:

>Translated_331_residues
MITVTLIPGDGIGPEITDVVVEVFEALGVDVDWEVVNAGKSVMDKYGTPLPDEVIESIKKNKVALKGPITTPVGSGFRSV
NVAIRKKLNLYANLRPVESLPGLKTRYDKVNLVVVRENTEGLYSGVEHKISDFAAESIKIITRKASERIIEFAFSYAKKN
DRQQVTAVHKANIMKISDGLFLDTARKVAEKYPGIEYNERIIDNMCMQLVQNPEDYDVLVMPNFYGDIVSDLGAGLVGGL
GVTPGANIGDEIAVFEAVHGSAPDIAGKGVANPVAILRSSIMLLNHIGESEKASKLDQAIKKTLVKGKGLTPDLGGQGTT
RSFKDQIINNI
>Mature_331_residues
MITVTLIPGDGIGPEITDVVVEVFEALGVDVDWEVVNAGKSVMDKYGTPLPDEVIESIKKNKVALKGPITTPVGSGFRSV
NVAIRKKLNLYANLRPVESLPGLKTRYDKVNLVVVRENTEGLYSGVEHKISDFAAESIKIITRKASERIIEFAFSYAKKN
DRQQVTAVHKANIMKISDGLFLDTARKVAEKYPGIEYNERIIDNMCMQLVQNPEDYDVLVMPNFYGDIVSDLGAGLVGGL
GVTPGANIGDEIAVFEAVHGSAPDIAGKGVANPVAILRSSIMLLNHIGESEKASKLDQAIKKTLVKGKGLTPDLGGQGTT
RSFKDQIINNI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=52.4242424242424, Blast_Score=357, Evalue=8e-99,
Organism=Homo sapiens, GI28178816, Length=329, Percent_Identity=46.5045592705167, Blast_Score=281, Evalue=6e-76,
Organism=Homo sapiens, GI28178821, Length=332, Percent_Identity=46.3855421686747, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI4758582, Length=337, Percent_Identity=45.1038575667656, Blast_Score=277, Evalue=9e-75,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=45.4838709677419, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=51.010101010101, Blast_Score=187, Evalue=9e-48,
Organism=Escherichia coli, GI1787381, Length=345, Percent_Identity=35.6521739130435, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=31.4606741573034, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI87081683, Length=329, Percent_Identity=31.6109422492401, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71986051, Length=329, Percent_Identity=51.9756838905775, Blast_Score=350, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI17550882, Length=335, Percent_Identity=42.089552238806, Blast_Score=267, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17505779, Length=331, Percent_Identity=42.5981873111783, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI25144293, Length=335, Percent_Identity=42.089552238806, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324709, Length=333, Percent_Identity=47.7477477477478, Blast_Score=326, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6324291, Length=333, Percent_Identity=47.1471471471471, Blast_Score=301, Evalue=7e-83,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=40.5714285714286, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=30.2197802197802, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643270, Length=327, Percent_Identity=53.822629969419, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24643268, Length=327, Percent_Identity=53.822629969419, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24661184, Length=334, Percent_Identity=48.502994011976, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI161078635, Length=327, Percent_Identity=43.7308868501529, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI161078637, Length=327, Percent_Identity=43.7308868501529, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI161078633, Length=327, Percent_Identity=43.7308868501529, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24650122, Length=327, Percent_Identity=43.7308868501529, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI161078639, Length=325, Percent_Identity=43.3846153846154, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI281362242, Length=334, Percent_Identity=47.3053892215569, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24648872, Length=334, Percent_Identity=47.3053892215569, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI20130355, Length=329, Percent_Identity=32.8267477203647, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI45550436, Length=331, Percent_Identity=24.1691842900302, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: 8180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35792; Mature: 35792

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVTLIPGDGIGPEITDVVVEVFEALGVDVDWEVVNAGKSVMDKYGTPLPDEVIESIKK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCCHHHHHHHHH
NKVALKGPITTPVGSGFRSVNVAIRKKLNLYANLRPVESLPGLKTRYDKVNLVVVRENTE
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEEECCCC
GLYSGVEHKISDFAAESIKIITRKASERIIEFAFSYAKKNDRQQVTAVHKANIMKISDGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCC
FLDTARKVAEKYPGIEYNERIIDNMCMQLVQNPEDYDVLVMPNFYGDIVSDLGAGLVGGL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCC
GVTPGANIGDEIAVFEAVHGSAPDIAGKGVANPVAILRSSIMLLNHIGESEKASKLDQAI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KKTLVKGKGLTPDLGGQGTTRSFKDQIINNI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MITVTLIPGDGIGPEITDVVVEVFEALGVDVDWEVVNAGKSVMDKYGTPLPDEVIESIKK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCCHHHHHHHHH
NKVALKGPITTPVGSGFRSVNVAIRKKLNLYANLRPVESLPGLKTRYDKVNLVVVRENTE
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEEECCCC
GLYSGVEHKISDFAAESIKIITRKASERIIEFAFSYAKKNDRQQVTAVHKANIMKISDGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCC
FLDTARKVAEKYPGIEYNERIIDNMCMQLVQNPEDYDVLVMPNFYGDIVSDLGAGLVGGL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCC
GVTPGANIGDEIAVFEAVHGSAPDIAGKGVANPVAILRSSIMLLNHIGESEKASKLDQAI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KKTLVKGKGLTPDLGGQGTTRSFKDQIINNI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]